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The map label for this gene is livK [C]

Identifier: 187930589

GI number: 187930589

Start: 3695999

End: 3697147

Strand: Reverse

Name: livK [C]

Synonym: Rpic_3525

Alternate gene names: 187930589

Gene position: 3697147-3695999 (Counterclockwise)

Preceding gene: 187930590

Following gene: 187930588

Centisome position: 93.78

GC content: 62.23

Gene sequence:

>1149_bases
ATGAAGGTTTCGCGCGCATCCCGCATTGCACTCGCCGCGACGGCACTGTTGGCAGGTACGGCGTTCGCCCAGGTGAAGGT
CGGCATCACGGTGTCTGCCACGGGGCCGGCCGCTTCCCTAGGCATTCCGGAAAAGAACACCGTCGCGCTGCTCCCCAAGG
AAATCGCCGGCAAGAAAATCGAGTACATCGTGCTCGACGATGCGTCTGACACGACCACGGCCGTCAAGAACACGCGCAAG
TTGATCACCGAAGACAAGGTCGACCTGGTGATCGGTTCGACCGTCACGCCGAACTCGCTGGCGATGGTGGATGTGGTGGC
CGAGAACGAAACGCCAATGATCACCTTGGCCGCATCAGCGCGGATCATCGAGCCGATGGACGCCAAGCGGGCGTGGGTCT
TCAAGACGCCGCAAAACGATTCCCACATGGCGACCGCCATTGCCGAGCACATGGCCAACCACGGCGTGAAGACGGTCGCG
CTGATCGGTTTTGCCGATGCCTATGGCGATTCGTGGGCGCAGGAGTTCGACAAGGTGGCTGCATTGCGCAAGCTGAAGGT
CGTCGCCAATGAGCGCTTTGCTCGGACCGATACGTCGGTGACGGGGCAGGTGCTGAAGATCATGTCAGCCAATCCGGATG
CGGTGCTGATCGCAGGCTCGGGTACGCCGGCCGCGTTGCCGGAACGGGCGCTCAAGGAGCGCGGCTACAAGGGCAAGCTG
TATCAGACGCACGGCGTGGCGAACGCCGACTTCCTGCGTGTGTGTGGCAAGGACTGCGAAGGCACGTACCTGCCCGCGGG
CCCGATCCTGGTTGCCGAGCAACTGCCGGACAGCAATCTGGTAAAGAAGCCGGCGCTGGCGTACAAGACTGCATATGAGA
AGGCCTACGGCGGGCAGGTTTCCACCTTCGGTGGCCACATGTGGGATGCCGGTTTGTTGTTCACGCAGGCGATGCCCGAA
GCGCTGAAGAAGGGGCAACCCGGCACGCCGGCGTTCCGCAAGGGGCTGCGCGATGCGATGGAGACGACGACCAACCTGGC
CATCTCGCACGGCGTGATGAATATGAATGCGAAGGATCACCTCGGGCTGGATCAGCGGGCGCGGGTGATGGTGGAGATCA
AGGACGGGAAGTGGAAGCTGCAGAATTAA

Upstream 100 bases:

>100_bases
GACTCCTTCTAGACTCGTTCATCCCCGACCCAGCGGTCGGCCAAATCGCAGATCCAGGGCGGGCGCAGACCCGCTTCGCA
TATCTCAGGAGACGGACGGC

Downstream 100 bases:

>100_bases
TGACGTAGCCCACGTCATTGCCCAAAGCAAAACGCACGGTTCGCGCCGTGCGTTTTGCTTTTGGGGCTCAAGTGCTGGCG
AGGCTTTTCGGTACTGGCGA

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKVSRASRIALAATALLAGTAFAQVKVGITVSATGPAASLGIPEKNTVALLPKEIAGKKIEYIVLDDASDTTTAVKNTRK
LITEDKVDLVIGSTVTPNSLAMVDVVAENETPMITLAASARIIEPMDAKRAWVFKTPQNDSHMATAIAEHMANHGVKTVA
LIGFADAYGDSWAQEFDKVAALRKLKVVANERFARTDTSVTGQVLKIMSANPDAVLIAGSGTPAALPERALKERGYKGKL
YQTHGVANADFLRVCGKDCEGTYLPAGPILVAEQLPDSNLVKKPALAYKTAYEKAYGGQVSTFGGHMWDAGLLFTQAMPE
ALKKGQPGTPAFRKGLRDAMETTTNLAISHGVMNMNAKDHLGLDQRARVMVEIKDGKWKLQN

Sequences:

>Translated_382_residues
MKVSRASRIALAATALLAGTAFAQVKVGITVSATGPAASLGIPEKNTVALLPKEIAGKKIEYIVLDDASDTTTAVKNTRK
LITEDKVDLVIGSTVTPNSLAMVDVVAENETPMITLAASARIIEPMDAKRAWVFKTPQNDSHMATAIAEHMANHGVKTVA
LIGFADAYGDSWAQEFDKVAALRKLKVVANERFARTDTSVTGQVLKIMSANPDAVLIAGSGTPAALPERALKERGYKGKL
YQTHGVANADFLRVCGKDCEGTYLPAGPILVAEQLPDSNLVKKPALAYKTAYEKAYGGQVSTFGGHMWDAGLLFTQAMPE
ALKKGQPGTPAFRKGLRDAMETTTNLAISHGVMNMNAKDHLGLDQRARVMVEIKDGKWKLQN
>Mature_382_residues
MKVSRASRIALAATALLAGTAFAQVKVGITVSATGPAASLGIPEKNTVALLPKEIAGKKIEYIVLDDASDTTTAVKNTRK
LITEDKVDLVIGSTVTPNSLAMVDVVAENETPMITLAASARIIEPMDAKRAWVFKTPQNDSHMATAIAEHMANHGVKTVA
LIGFADAYGDSWAQEFDKVAALRKLKVVANERFARTDTSVTGQVLKIMSANPDAVLIAGSGTPAALPERALKERGYKGKL
YQTHGVANADFLRVCGKDCEGTYLPAGPILVAEQLPDSNLVKKPALAYKTAYEKAYGGQVSTFGGHMWDAGLLFTQAMPE
ALKKGQPGTPAFRKGLRDAMETTTNLAISHGVMNMNAKDHLGLDQRARVMVEIKDGKWKLQN

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40786; Mature: 40786

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSRASRIALAATALLAGTAFAQVKVGITVSATGPAASLGIPEKNTVALLPKEIAGKKI
CCCCHHHHHHHHHHHHHHHCEEEEEEEEEEEECCCCCCCCCCCCCCEEEECCHHHCCCEE
EYIVLDDASDTTTAVKNTRKLITEDKVDLVIGSTVTPNSLAMVDVVAENETPMITLAASA
EEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEECCCCCEEEEECCC
RIIEPMDAKRAWVFKTPQNDSHMATAIAEHMANHGVKTVALIGFADAYGDSWAQEFDKVA
EEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEEEHHHHCCHHHHHHHHHH
ALRKLKVVANERFARTDTSVTGQVLKIMSANPDAVLIAGSGTPAALPERALKERGYKGKL
HHHHHHHHHCCCHHCCCCHHHHHHHHEECCCCCEEEEECCCCCCCCHHHHHHHCCCCCCE
YQTHGVANADFLRVCGKDCEGTYLPAGPILVAEQLPDSNLVKKPALAYKTAYEKAYGGQV
EEECCCCCHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCE
STFGGHMWDAGLLFTQAMPEALKKGQPGTPAFRKGLRDAMETTTNLAISHGVMNMNAKDH
ECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEECCEEECCCHHC
LGLDQRARVMVEIKDGKWKLQN
CCCCCCCEEEEEEECCEEEECC
>Mature Secondary Structure
MKVSRASRIALAATALLAGTAFAQVKVGITVSATGPAASLGIPEKNTVALLPKEIAGKKI
CCCCHHHHHHHHHHHHHHHCEEEEEEEEEEEECCCCCCCCCCCCCCEEEECCHHHCCCEE
EYIVLDDASDTTTAVKNTRKLITEDKVDLVIGSTVTPNSLAMVDVVAENETPMITLAASA
EEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEECCCCCEEEEECCC
RIIEPMDAKRAWVFKTPQNDSHMATAIAEHMANHGVKTVALIGFADAYGDSWAQEFDKVA
EEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEEEHHHHCCHHHHHHHHHH
ALRKLKVVANERFARTDTSVTGQVLKIMSANPDAVLIAGSGTPAALPERALKERGYKGKL
HHHHHHHHHCCCHHCCCCHHHHHHHHEECCCCCEEEEECCCCCCCCHHHHHHHCCCCCCE
YQTHGVANADFLRVCGKDCEGTYLPAGPILVAEQLPDSNLVKKPALAYKTAYEKAYGGQV
EEECCCCCHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCE
STFGGHMWDAGLLFTQAMPEALKKGQPGTPAFRKGLRDAMETTTNLAISHGVMNMNAKDH
ECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEECCEEECCCHHC
LGLDQRARVMVEIKDGKWKLQN
CCCCCCCEEEEEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]