Click here to switch to the map view.

The map label for this gene is gidB [H]

Identifier: 187930587

GI number: 187930587

Start: 3692620

End: 3693288

Strand: Reverse

Name: gidB [H]

Synonym: Rpic_3522

Alternate gene names: 187930587

Gene position: 3693288-3692620 (Counterclockwise)

Preceding gene: 187930588

Following gene: 187930586

Centisome position: 93.68

GC content: 66.37

Gene sequence:

>669_bases
ATGGCGAATGCACTGACAGACGCCCGGGCAGAGTTGGAAGCCGGCGCCCGTGCGCTTGGCTTGTCGCTGGACGACGCGCA
GGTCGATCGCCTGCTGGCGTATCAAGGGCTGCTGGCCAAATGGAACCGCGTCTATAACCTGACCGCCATTCGTGATGCCG
GCGACATGCTGACGCATCACCTGCTGGATTCGCTCTCGGCCGTGTCGCGCATCGCCGGTTTGGCCCGCCGTGCCCAACCC
GACGCACCGCGCGTGCTGGACGTCGGTTCGGGTGGTGGGCTGCCGGGCATTCCGCTGGCCATCGCTTTCCCAGATGTAAG
CGTCACGATGGTCGACATCGTGCAGAAGAAGACCGCCTTCCTGACGCAGTGCCGCGCCGAGCTTGGCTTGGCCAATGCCC
AATCGCATTGGGGCCATGTAGAAAAACTGGCCGATGCCACCGGCTACGGCGTCATCACGTCACGCGCGTTTGCCGAGTTG
GTCGATTTCGTGAATCTGGCCGGGCACCTGCTGGCGCCCGGCGGCCGCATGGTCGCCATGAAGGGCGTGCGCCCGGATGC
CGAAATCGAACGCCTGCCCGCCGGCTGGACCGTCGAAGCCATCGAGCGCCTGACCGTGCCGGGGCTGCCGGCTGAGCGTC
ACCTGGTCATCCTCGCGCCGTCGGCGTGA

Upstream 100 bases:

>100_bases
TCACGCCGGCCGCCGTGTCGCTGCTGCTCGTGCACCTCAAGAAGGGCGTGCTGCGTGGCCAAGTTGGCCCGCGCGCCAGT
TCAGAAGGCGAGGCCGCTTG

Downstream 100 bases:

>100_bases
GCGAACGTTGTAGCGAGCCATTCACCGCAGAGGAGTCATGTCGAATATTTTCGTGATCGCCAACCAGAAGGGCGGGGTCG
GCAAGACCACCACCACGGTG

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MANALTDARAELEAGARALGLSLDDAQVDRLLAYQGLLAKWNRVYNLTAIRDAGDMLTHHLLDSLSAVSRIAGLARRAQP
DAPRVLDVGSGGGLPGIPLAIAFPDVSVTMVDIVQKKTAFLTQCRAELGLANAQSHWGHVEKLADATGYGVITSRAFAEL
VDFVNLAGHLLAPGGRMVAMKGVRPDAEIERLPAGWTVEAIERLTVPGLPAERHLVILAPSA

Sequences:

>Translated_222_residues
MANALTDARAELEAGARALGLSLDDAQVDRLLAYQGLLAKWNRVYNLTAIRDAGDMLTHHLLDSLSAVSRIAGLARRAQP
DAPRVLDVGSGGGLPGIPLAIAFPDVSVTMVDIVQKKTAFLTQCRAELGLANAQSHWGHVEKLADATGYGVITSRAFAEL
VDFVNLAGHLLAPGGRMVAMKGVRPDAEIERLPAGWTVEAIERLTVPGLPAERHLVILAPSA
>Mature_221_residues
ANALTDARAELEAGARALGLSLDDAQVDRLLAYQGLLAKWNRVYNLTAIRDAGDMLTHHLLDSLSAVSRIAGLARRAQPD
APRVLDVGSGGGLPGIPLAIAFPDVSVTMVDIVQKKTAFLTQCRAELGLANAQSHWGHVEKLADATGYGVITSRAFAELV
DFVNLAGHLLAPGGRMVAMKGVRPDAEIERLPAGWTVEAIERLTVPGLPAERHLVILAPSA

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=199, Percent_Identity=41.2060301507538, Blast_Score=160, Evalue=9e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: =2.1.1.170 [H]

Molecular weight: Translated: 23492; Mature: 23361

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANALTDARAELEAGARALGLSLDDAQVDRLLAYQGLLAKWNRVYNLTAIRDAGDMLTHH
CCCCHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHH
LLDSLSAVSRIAGLARRAQPDAPRVLDVGSGGGLPGIPLAIAFPDVSVTMVDIVQKKTAF
HHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
LTQCRAELGLANAQSHWGHVEKLADATGYGVITSRAFAELVDFVNLAGHLLAPGGRMVAM
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
KGVRPDAEIERLPAGWTVEAIERLTVPGLPAERHLVILAPSA
ECCCCCHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEECCC
>Mature Secondary Structure 
ANALTDARAELEAGARALGLSLDDAQVDRLLAYQGLLAKWNRVYNLTAIRDAGDMLTHH
CCCHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHH
LLDSLSAVSRIAGLARRAQPDAPRVLDVGSGGGLPGIPLAIAFPDVSVTMVDIVQKKTAF
HHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
LTQCRAELGLANAQSHWGHVEKLADATGYGVITSRAFAELVDFVNLAGHLLAPGGRMVAM
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
KGVRPDAEIERLPAGWTVEAIERLTVPGLPAERHLVILAPSA
ECCCCCHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]