Click here to switch to the map view.

The map label for this gene is gltD [H]

Identifier: 187930309

GI number: 187930309

Start: 3386671

End: 3388134

Strand: Reverse

Name: gltD [H]

Synonym: Rpic_3242

Alternate gene names: 187930309

Gene position: 3388134-3386671 (Counterclockwise)

Preceding gene: 187930310

Following gene: 187930308

Centisome position: 85.94

GC content: 64.34

Gene sequence:

>1464_bases
ATGGGCAAGGCGACCGGTTTTCTCGAATTTCCCCGCCAGAACGAGGCATACGAAGCGCCCGAAGCGCGTGTGAAGCATTA
CAAGGAATTCGTGTTCGCACTGGCGGACAACGAAGCCAAGATCCAGGGCGCACGCTGCATGGACTGCGGCATTCCGTTCT
GCAACAACGGCTGCCCGGTCAACAACATCATCCCGGACTTCAACGACCTGGTGTATCGCCAGGACTGGAAGACCGCGATT
GAAGTGCTGCACTCGACCAACAACTTCCCCGAGTTCACGGGCCGCATCTGCCCGGCACCGTGCGAGGCGGCTTGCACGCT
GGGCATTAATGAACTGCCGGTCGGTATCAAGTCGATCGAGCACGCGATCATCGACAAGGCCTGGAAAGAAGGCTGGGTCG
CGCCGCAAGTGCCCAAGCACAAGACCGGCAAGACCGTGGCCGTGGTCGGTTCGGGCCCGGCTGGTATGGCCGCTGCGCAG
CAGCTGGCACGCGCTGGCCACGACGTGACGCTGTTCGAAAAGAACGACCGCATCGGCGGCCTGCTGCGCTACGGCATTCC
CGACTTCAAGCTGGAAAAGGCGCAGATCGACCGCCGCATGCAGCAGATGGAAGCCGAGGGCGTGACGTTCCGCACCGGCG
TGATGGTGGGCGACAAGACGGTTCCGGCCGGCATCACCAACGACGCACGCGAGACCATCGCCGCGGAAGACATCCTCAAG
CAATTCGACGCCGTCGTGCTGACGGGCGGATCGGAAGTGCCGCGCGATCTGCCAGTGCCGGGCCGTGACCTGGACGGCGT
GCATTACGCGCTCGAATTTCTGATCCCGCAGAACAAGGAAGTGGCCGGCGACGTGCCCAACCCGATCCGCGCCGACGGCA
AGCACGTCATCGTGATCGGCGGCGGCGACACGGGTTCGGACTGCGTGGGCACGTCCAACCGCCATGGCGCCGCATCGGTC
ACGCAGTTCGAACTGCTGCCGCGTCCGCCCGAAGAAGAGAACAAGCCGCTGGTGTGGCCGTACTGGCCGATCAAGTTGCG
CACGTCGTCGTCGCATGATGAAGGCTGCGAGCGCGACTGGTCGGTCGCAACCAAGGCGCTCATCGGCGAGAACGGCCGCG
TGACGGGCCTCACCGCCGTGCGCCTTGAGTGGAAGGACGGCAAGATGGTCGAGGTCGCGGGCAGCGAATTCACCCTCAAG
GCCGATCTGGTTCTGTTGGCGATGGGCTTTACCAACCCGGTCGGTGGTGTGCTGGATGCCTTTGGCGTGACAAAGGACGC
GCGCAACAACGCGCGTGCCGCCACCGAAGGCGACAACGCTTACGCCACCAACGTGCCGAAGGTGTTCGCTGCCGGCGATG
TGCGCCGCGGCCAGTCGCTCGTCGTGTGGGCGATCCGGGAAGGCCGCCAGGCTGCCCGCTCGGTTGATGCGTTTCTCATG
GGTCATTCGGAACTGCCGCGCTGA

Upstream 100 bases:

>100_bases
GTTCGCCACCACCGAATACAACGCAACCTCACAAGACAGGCCCGACTGCCTGGCTGGTACGACTGTGCCGGCAGAGCAGC
ATCGGGTCAGGAAGGACACC

Downstream 100 bases:

>100_bases
CCACGCGGTTGACCGTTTGACTCAGGCCCGCCTGCCTCGGCGGGCCTTTTTGTTTGTGCCGCGTTTGCTGTGGCGCGTTT
GCTGTGGCGCGAGCGTCATG

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MGKATGFLEFPRQNEAYEAPEARVKHYKEFVFALADNEAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWKTAI
EVLHSTNNFPEFTGRICPAPCEAACTLGINELPVGIKSIEHAIIDKAWKEGWVAPQVPKHKTGKTVAVVGSGPAGMAAAQ
QLARAGHDVTLFEKNDRIGGLLRYGIPDFKLEKAQIDRRMQQMEAEGVTFRTGVMVGDKTVPAGITNDARETIAAEDILK
QFDAVVLTGGSEVPRDLPVPGRDLDGVHYALEFLIPQNKEVAGDVPNPIRADGKHVIVIGGGDTGSDCVGTSNRHGAASV
TQFELLPRPPEEENKPLVWPYWPIKLRTSSSHDEGCERDWSVATKALIGENGRVTGLTAVRLEWKDGKMVEVAGSEFTLK
ADLVLLAMGFTNPVGGVLDAFGVTKDARNNARAATEGDNAYATNVPKVFAAGDVRRGQSLVVWAIREGRQAARSVDAFLM
GHSELPR

Sequences:

>Translated_487_residues
MGKATGFLEFPRQNEAYEAPEARVKHYKEFVFALADNEAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWKTAI
EVLHSTNNFPEFTGRICPAPCEAACTLGINELPVGIKSIEHAIIDKAWKEGWVAPQVPKHKTGKTVAVVGSGPAGMAAAQ
QLARAGHDVTLFEKNDRIGGLLRYGIPDFKLEKAQIDRRMQQMEAEGVTFRTGVMVGDKTVPAGITNDARETIAAEDILK
QFDAVVLTGGSEVPRDLPVPGRDLDGVHYALEFLIPQNKEVAGDVPNPIRADGKHVIVIGGGDTGSDCVGTSNRHGAASV
TQFELLPRPPEEENKPLVWPYWPIKLRTSSSHDEGCERDWSVATKALIGENGRVTGLTAVRLEWKDGKMVEVAGSEFTLK
ADLVLLAMGFTNPVGGVLDAFGVTKDARNNARAATEGDNAYATNVPKVFAAGDVRRGQSLVVWAIREGRQAARSVDAFLM
GHSELPR
>Mature_486_residues
GKATGFLEFPRQNEAYEAPEARVKHYKEFVFALADNEAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWKTAIE
VLHSTNNFPEFTGRICPAPCEAACTLGINELPVGIKSIEHAIIDKAWKEGWVAPQVPKHKTGKTVAVVGSGPAGMAAAQQ
LARAGHDVTLFEKNDRIGGLLRYGIPDFKLEKAQIDRRMQQMEAEGVTFRTGVMVGDKTVPAGITNDARETIAAEDILKQ
FDAVVLTGGSEVPRDLPVPGRDLDGVHYALEFLIPQNKEVAGDVPNPIRADGKHVIVIGGGDTGSDCVGTSNRHGAASVT
QFELLPRPPEEENKPLVWPYWPIKLRTSSSHDEGCERDWSVATKALIGENGRVTGLTAVRLEWKDGKMVEVAGSEFTLKA
DLVLLAMGFTNPVGGVLDAFGVTKDARNNARAATEGDNAYATNVPKVFAAGDVRRGQSLVVWAIREGRQAARSVDAFLMG
HSELPR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=474, Percent_Identity=25.3164556962025, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI1789606, Length=487, Percent_Identity=35.9342915811088, Blast_Score=258, Evalue=8e-70,
Organism=Escherichia coli, GI1788811, Length=476, Percent_Identity=36.344537815126, Blast_Score=252, Evalue=5e-68,
Organism=Escherichia coli, GI87082180, Length=471, Percent_Identity=33.9702760084926, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1788468, Length=476, Percent_Identity=28.5714285714286, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI1789243, Length=433, Percent_Identity=26.5588914549654, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17570289, Length=506, Percent_Identity=44.6640316205534, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI71984108, Length=473, Percent_Identity=25.5813953488372, Blast_Score=93, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6320030, Length=501, Percent_Identity=48.1037924151697, Blast_Score=458, Evalue=1e-130,
Organism=Drosophila melanogaster, GI28574881, Length=502, Percent_Identity=49.402390438247, Blast_Score=483, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24665539, Length=502, Percent_Identity=49.402390438247, Blast_Score=483, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24665547, Length=502, Percent_Identity=49.402390438247, Blast_Score=481, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24665543, Length=502, Percent_Identity=49.402390438247, Blast_Score=481, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24640763, Length=484, Percent_Identity=23.5537190082645, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI18858217, Length=484, Percent_Identity=23.5537190082645, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52792; Mature: 52661

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKATGFLEFPRQNEAYEAPEARVKHYKEFVFALADNEAKIQGARCMDCGIPFCNNGCPV
CCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCEEEECCCCCCCCCCCC
NNIIPDFNDLVYRQDWKTAIEVLHSTNNFPEFTGRICPAPCEAACTLGINELPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCHHEECCCCCCCCCHHHHH
HAIIDKAWKEGWVAPQVPKHKTGKTVAVVGSGPAGMAAAQQLARAGHDVTLFEKNDRIGG
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKLEKAQIDRRMQQMEAEGVTFRTGVMVGDKTVPAGITNDARETIAAEDILK
EEECCCCCCEEHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
QFDAVVLTGGSEVPRDLPVPGRDLDGVHYALEFLIPQNKEVAGDVPNPIRADGKHVIVIG
HCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEE
GGDTGSDCVGTSNRHGAASVTQFELLPRPPEEENKPLVWPYWPIKLRTSSSHDEGCERDW
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEECCCCEEEECCCCCCCCCCCHH
SVATKALIGENGRVTGLTAVRLEWKDGKMVEVAGSEFTLKADLVLLAMGFTNPVGGVLDA
HHHHHHHHCCCCCEEEEEEEEEEECCCCEEEECCCCEEEEEEEEEEEECCCCCHHHHHHH
FGVTKDARNNARAATEGDNAYATNVPKVFAAGDVRRGQSLVVWAIREGRQAARSVDAFLM
HCCCCCCCCCCCCCCCCCCCEECCCCCEEECCCCCCCCEEEEEEEHHHHHHHHHHHHHHC
GHSELPR
CCCCCCC
>Mature Secondary Structure 
GKATGFLEFPRQNEAYEAPEARVKHYKEFVFALADNEAKIQGARCMDCGIPFCNNGCPV
CCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCEEEECCCCCCCCCCCC
NNIIPDFNDLVYRQDWKTAIEVLHSTNNFPEFTGRICPAPCEAACTLGINELPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCHHEECCCCCCCCCHHHHH
HAIIDKAWKEGWVAPQVPKHKTGKTVAVVGSGPAGMAAAQQLARAGHDVTLFEKNDRIGG
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKLEKAQIDRRMQQMEAEGVTFRTGVMVGDKTVPAGITNDARETIAAEDILK
EEECCCCCCEEHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
QFDAVVLTGGSEVPRDLPVPGRDLDGVHYALEFLIPQNKEVAGDVPNPIRADGKHVIVIG
HCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEE
GGDTGSDCVGTSNRHGAASVTQFELLPRPPEEENKPLVWPYWPIKLRTSSSHDEGCERDW
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEECCCCEEEECCCCCCCCCCCHH
SVATKALIGENGRVTGLTAVRLEWKDGKMVEVAGSEFTLKADLVLLAMGFTNPVGGVLDA
HHHHHHHHCCCCCEEEEEEEEEEECCCCEEEECCCCEEEEEEEEEEEECCCCCHHHHHHH
FGVTKDARNNARAATEGDNAYATNVPKVFAAGDVRRGQSLVVWAIREGRQAARSVDAFLM
HCCCCCCCCCCCCCCCCCCCEECCCCCEEECCCCCCCCEEEEEEEHHHHHHHHHHHHHHC
GHSELPR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]