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The map label for this gene is hsdM [C]

Identifier: 187930245

GI number: 187930245

Start: 3332548

End: 3334044

Strand: Reverse

Name: hsdM [C]

Synonym: Rpic_3178

Alternate gene names: 187930245

Gene position: 3334044-3332548 (Counterclockwise)

Preceding gene: 187930246

Following gene: 187930244

Centisome position: 84.57

GC content: 58.98

Gene sequence:

>1497_bases
TTGAGCAACCCACAAATTACTCTCAGTCAGCTTGAAGGCCACCTATGGGAGTCCGCCAACATTCTGCGCGGCCCGGTTGA
TGCTGCTGACTTCAAGACTTACATCTTCCCTCTGCTGTTTTTCAAGCGCATCTGCGACGTCTGGGACGAGGAATACCAGG
AGATCGTTGACGAGACGGGCGATGAGGAACTGGCCTGGTTCCCGGAGTCACACCGCTTTCAGATCCCAGAGGATTGCCAC
TGGAACGATGTTCGCGCCAAAGCGAGCAATGTCGGTACCGCGCTGCAGCGCGCGATGCGGGAAATTGAGAGAGCCAACCC
GGACACCCTATATGGCGTGTTCGGCGATGCCCAGTGGTCAAACAAGGAGAGGCTGTCGGATGCCCTCCTCAAAGACCTGA
TCGAACATTTTTCCAAGCTGCCGTTTGGCAACAAGAACGTCAACTCGGATCTGCTCGGCGACGCCTACGAATACCTGATC
AAAAAATTCGCCGACGCCACCAACAAAAAGGCCGGCGAGTTCTACACCCCACGCAGTGTGGTGCGGCTGATGATCGACAT
GCTCGATCCCAAGGAGGCCGAGACCATCTACGACCCGGCCTGTGGCACCGGCGGCATGTTGCTGGCAGCCGTGCAACACG
TCAAAGAGATGCACGGCGACGTCAAGCGGCTGTGGGGTAAGCTATACGGGCAGGAGAAAAACCTCACCACTTCATCCATC
GCGCGGATGAACCTGTTCCTTCACGGCATCGAGGACTTCCAGGTGGTGCGCGGAGACACGCTGCGCAACCCGGCCTTCTT
CGAGGGCGACCGACTGGCTACCTTCGACTGCGTGATCGCCAACCCGCCGTTCTCTTTGGAAAAGTGGGGCGAGGACCTGT
GGTTGAACGATCCCTTTGGTCGCAACTTTGCCGGTCTGCCGCCCTCGTCCAGCGGTGACTTCGCCTGGGTGCAGCACATG
GTCAAGTCGATGGCCGACGCGACCGGCCGCATGGCAGTGGTGCTACCACAAGGCGCCCTGTTCCGCAAAGGCGTCGAAGG
CAGCATCCGCCAGAAACTACTGGAAATGGATCTAGTCGATGCCGTGATCGGTCTGGCGCCGAATCTGTTCTACGGCACGG
GACTCGCCGCTTGCATCCTGTTGCTGCGCAAGCGCAAGTCGGCTAAGCACAAGAAGAAGGTGCTGATCGCCGATGCGTCG
CGCCTGTTCCGCCGGGGGCGCGCGCAGAACTATCTGGAGGCCGAGCACGCCGCTGAGATCCTCGGGTGGTATCGCGGCTT
TGCCAATGTGCAGGATGCGGTTCGAGTGGTCAGCCTCGATGAGATTAAGGCCGAGGACTGGACACTGAACATTTCGCGCT
ACGTGTTACCACCGCTGCGGGAAGACATTCCTCCTTTGCCAGTCGCCATTGCATCCTTCAAGGATGCACTCACCCACTGC
CGCGAGGCAGAGGAACGGCTCGCGCAGGTTATGACCAAAGGAGGATGGCTTAAATGA

Upstream 100 bases:

>100_bases
ACTACATCGTCAAACAGTCCAGCCTTGTAGAGTAGTCGTTGAATACCAATCAAGAACACACGCCGCAGTGCTACGGCATG
GATGAAATAGGAGCATGTGT

Downstream 100 bases:

>100_bases
GCATGTCGGTGCCGGAACTGAAGTGGGCATTCCCAATAGATCCCGCGTATTCATGGTTTGAGAGCTTTGCTGATCAATTC
GCCAGTCCAGATGTCGTATT

Product: N-6 DNA methylase

Products: NA

Alternate protein names: M.MjaXP [H]

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MSNPQITLSQLEGHLWESANILRGPVDAADFKTYIFPLLFFKRICDVWDEEYQEIVDETGDEELAWFPESHRFQIPEDCH
WNDVRAKASNVGTALQRAMREIERANPDTLYGVFGDAQWSNKERLSDALLKDLIEHFSKLPFGNKNVNSDLLGDAYEYLI
KKFADATNKKAGEFYTPRSVVRLMIDMLDPKEAETIYDPACGTGGMLLAAVQHVKEMHGDVKRLWGKLYGQEKNLTTSSI
ARMNLFLHGIEDFQVVRGDTLRNPAFFEGDRLATFDCVIANPPFSLEKWGEDLWLNDPFGRNFAGLPPSSSGDFAWVQHM
VKSMADATGRMAVVLPQGALFRKGVEGSIRQKLLEMDLVDAVIGLAPNLFYGTGLAACILLLRKRKSAKHKKKVLIADAS
RLFRRGRAQNYLEAEHAAEILGWYRGFANVQDAVRVVSLDEIKAEDWTLNISRYVLPPLREDIPPLPVAIASFKDALTHC
REAEERLAQVMTKGGWLK

Sequences:

>Translated_498_residues
MSNPQITLSQLEGHLWESANILRGPVDAADFKTYIFPLLFFKRICDVWDEEYQEIVDETGDEELAWFPESHRFQIPEDCH
WNDVRAKASNVGTALQRAMREIERANPDTLYGVFGDAQWSNKERLSDALLKDLIEHFSKLPFGNKNVNSDLLGDAYEYLI
KKFADATNKKAGEFYTPRSVVRLMIDMLDPKEAETIYDPACGTGGMLLAAVQHVKEMHGDVKRLWGKLYGQEKNLTTSSI
ARMNLFLHGIEDFQVVRGDTLRNPAFFEGDRLATFDCVIANPPFSLEKWGEDLWLNDPFGRNFAGLPPSSSGDFAWVQHM
VKSMADATGRMAVVLPQGALFRKGVEGSIRQKLLEMDLVDAVIGLAPNLFYGTGLAACILLLRKRKSAKHKKKVLIADAS
RLFRRGRAQNYLEAEHAAEILGWYRGFANVQDAVRVVSLDEIKAEDWTLNISRYVLPPLREDIPPLPVAIASFKDALTHC
REAEERLAQVMTKGGWLK
>Mature_497_residues
SNPQITLSQLEGHLWESANILRGPVDAADFKTYIFPLLFFKRICDVWDEEYQEIVDETGDEELAWFPESHRFQIPEDCHW
NDVRAKASNVGTALQRAMREIERANPDTLYGVFGDAQWSNKERLSDALLKDLIEHFSKLPFGNKNVNSDLLGDAYEYLIK
KFADATNKKAGEFYTPRSVVRLMIDMLDPKEAETIYDPACGTGGMLLAAVQHVKEMHGDVKRLWGKLYGQEKNLTTSSIA
RMNLFLHGIEDFQVVRGDTLRNPAFFEGDRLATFDCVIANPPFSLEKWGEDLWLNDPFGRNFAGLPPSSSGDFAWVQHMV
KSMADATGRMAVVLPQGALFRKGVEGSIRQKLLEMDLVDAVIGLAPNLFYGTGLAACILLLRKRKSAKHKKKVLIADASR
LFRRGRAQNYLEAEHAAEILGWYRGFANVQDAVRVVSLDEIKAEDWTLNISRYVLPPLREDIPPLPVAIASFKDALTHCR
EAEERLAQVMTKGGWLK

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=391, Percent_Identity=26.8542199488491, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 56239; Mature: 56108

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNPQITLSQLEGHLWESANILRGPVDAADFKTYIFPLLFFKRICDVWDEEYQEIVDETG
CCCCCEEHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DEELAWFPESHRFQIPEDCHWNDVRAKASNVGTALQRAMREIERANPDTLYGVFGDAQWS
CCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
NKERLSDALLKDLIEHFSKLPFGNKNVNSDLLGDAYEYLIKKFADATNKKAGEFYTPRSV
CHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
VRLMIDMLDPKEAETIYDPACGTGGMLLAAVQHVKEMHGDVKRLWGKLYGQEKNLTTSSI
HHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
ARMNLFLHGIEDFQVVRGDTLRNPAFFEGDRLATFDCVIANPPFSLEKWGEDLWLNDPFG
HHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHCCCCCCCCCCCC
RNFAGLPPSSSGDFAWVQHMVKSMADATGRMAVVLPQGALFRKGVEGSIRQKLLEMDLVD
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCHHHHHHHHHHHHHH
AVIGLAPNLFYGTGLAACILLLRKRKSAKHKKKVLIADASRLFRRGRAQNYLEAEHAAEI
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LGWYRGFANVQDAVRVVSLDEIKAEDWTLNISRYVLPPLREDIPPLPVAIASFKDALTHC
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCHHHHCCCCCHHHHHHHHHHHHHH
REAEERLAQVMTKGGWLK
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SNPQITLSQLEGHLWESANILRGPVDAADFKTYIFPLLFFKRICDVWDEEYQEIVDETG
CCCCEEHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DEELAWFPESHRFQIPEDCHWNDVRAKASNVGTALQRAMREIERANPDTLYGVFGDAQWS
CCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
NKERLSDALLKDLIEHFSKLPFGNKNVNSDLLGDAYEYLIKKFADATNKKAGEFYTPRSV
CHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
VRLMIDMLDPKEAETIYDPACGTGGMLLAAVQHVKEMHGDVKRLWGKLYGQEKNLTTSSI
HHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
ARMNLFLHGIEDFQVVRGDTLRNPAFFEGDRLATFDCVIANPPFSLEKWGEDLWLNDPFG
HHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHCCCCCCCCCCCC
RNFAGLPPSSSGDFAWVQHMVKSMADATGRMAVVLPQGALFRKGVEGSIRQKLLEMDLVD
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCHHHHHHHHHHHHHH
AVIGLAPNLFYGTGLAACILLLRKRKSAKHKKKVLIADASRLFRRGRAQNYLEAEHAAEI
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LGWYRGFANVQDAVRVVSLDEIKAEDWTLNISRYVLPPLREDIPPLPVAIASFKDALTHC
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCHHHHCCCCCHHHHHHHHHHHHHH
REAEERLAQVMTKGGWLK
HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]