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The map label for this gene is mpl [H]

Identifier: 187930094

GI number: 187930094

Start: 3154158

End: 3155543

Strand: Reverse

Name: mpl [H]

Synonym: Rpic_3026

Alternate gene names: 187930094

Gene position: 3155543-3154158 (Counterclockwise)

Preceding gene: 187930106

Following gene: 187930093

Centisome position: 80.04

GC content: 61.83

Gene sequence:

>1386_bases
ATGCATATCCACATCCTTGGAATCTGCGGCACCTTCATGGGCGGCATTGCCCTGATCGCCCGTCAGGCCGGCCACCGCGT
CACCGGCTGCGACGCCAACGTCTACCCTCCGATGAGCACCCAGCTCGAAGCGCAAGGCATCGAGCTGATCGAAGGCTTTG
ACCCCGAACAGCTCTCCATCGGCGCCGATCTTTACGTGATCGGCAACGTGATCTCCCGTGGCAACGCTCTCATGGAAGCC
ATCCTGGATCGCAATTTGCCCTACATCTCCGGTCCTCAATGGCTCGGCGACAACGTACTACGCCAGAAGTGGGTGCTTGC
CGTGGCAGGCACACACGGCAAGACGACCACGACATCGATGCTCGCGTGGATCCTGCAGGATGCCGGCTACAACCCGGGCT
TCCTGGTGGGCGGCGTGCCACGCAACTTCGGGCTGTCGGCGCGCCTGACGGAATCCGACTTCTTCGTGATTGAAGCGGAC
GAGTACGACACGGCCTTCTTCGACAAGCGCAGCAAGTTCGTTCATTACCGCCCCCGTACAGCCATATTGAACAACCTCGA
ATTCGATCACGCCGACATCTTTCCCGATCTTGCCGCGATCGAAACCCAATTTCATCATCTGGTGCGCACCGTGCCCGGTC
AGGGCCGCCTCCTGGTCAATGGTCGGGAAGATAGCCTGAAGCGTGTACTTGAGCGCGGATGTTGGTCGGAAGTCGAGCAG
TTCGGGGCGGAGGGCAACCTCGGCTGGGAAGTCGAAAACCTCGCCGATGACGATAGCTTCGATGTCGTTTTCCAGGGCCA
AACGCAGGGCAGGGTCCGGTGGGCACTTCAGGGCGACCACAACCGCATGAACGCGCTGGCCGCCATTGCCGCCGCCCGCC
ACGTGGGCGTGCCGCCTGCCCAAGCCATTGAAAGCCTGGCCCGTTTCGAAAATGTGAAACGGCGCATGGAAGTGCGTGGC
ACCGTGAATGACGTGACTGTGTACGATGATTTTGCCCATCACCCGACGGCGATTCGCACCACCATCGAGGGCCTGCGCCG
TCGTGTCGGCAAGGGGCGCATCCTGGCAGTGCTGGAGCCACGGTCAAACACCATGAAGTTGGGCGTGATGAAACTGGCCC
TTCCCGACAGCCTGTCCGGCGCTGAACAGATCTTCGCTTATGGCGCAGCCAATGGCCGCGACGCCCTCGGCTGGGATTTG
GCTGGGGCGCTGGCCCCGCTGGGGGAGCGCGCGAAGACGTTTTCTGATCTGAGCGCATTAATCGAAGCCGTCACAGCTGC
CGCCCGCCCGGGCGACCATGTGCTTGTCATGAGCAACGGCGGCTTCGGAGGCGTACATCAGAAGCTGCTGGACGCCTTGG
CCGAACGCGCCGAGACAGCGAATTGA

Upstream 100 bases:

>100_bases
CGAGGGTGGGTCGTTTGGACATGGCTCTATTTTACTGAAGCGCGCGGGCCGCCCTGAAGCCGCAGGTACAATGCGGCGCA
ACGAAGTTGAGATGGCTCCC

Downstream 100 bases:

>100_bases
ATCTCATATGCTGGCATCCATCGTCTATCTGCACGGATTTCGTTCGTCGCCGCGCTCGTTCAAAGCGCAGCTGCTAGGCA
GGCGGATGGCGTCCGTAGGG

Product: UDP-N-acetylmuramate

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MHIHILGICGTFMGGIALIARQAGHRVTGCDANVYPPMSTQLEAQGIELIEGFDPEQLSIGADLYVIGNVISRGNALMEA
ILDRNLPYISGPQWLGDNVLRQKWVLAVAGTHGKTTTTSMLAWILQDAGYNPGFLVGGVPRNFGLSARLTESDFFVIEAD
EYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLAAIETQFHHLVRTVPGQGRLLVNGREDSLKRVLERGCWSEVEQ
FGAEGNLGWEVENLADDDSFDVVFQGQTQGRVRWALQGDHNRMNALAAIAAARHVGVPPAQAIESLARFENVKRRMEVRG
TVNDVTVYDDFAHHPTAIRTTIEGLRRRVGKGRILAVLEPRSNTMKLGVMKLALPDSLSGAEQIFAYGAANGRDALGWDL
AGALAPLGERAKTFSDLSALIEAVTAAARPGDHVLVMSNGGFGGVHQKLLDALAERAETAN

Sequences:

>Translated_461_residues
MHIHILGICGTFMGGIALIARQAGHRVTGCDANVYPPMSTQLEAQGIELIEGFDPEQLSIGADLYVIGNVISRGNALMEA
ILDRNLPYISGPQWLGDNVLRQKWVLAVAGTHGKTTTTSMLAWILQDAGYNPGFLVGGVPRNFGLSARLTESDFFVIEAD
EYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLAAIETQFHHLVRTVPGQGRLLVNGREDSLKRVLERGCWSEVEQ
FGAEGNLGWEVENLADDDSFDVVFQGQTQGRVRWALQGDHNRMNALAAIAAARHVGVPPAQAIESLARFENVKRRMEVRG
TVNDVTVYDDFAHHPTAIRTTIEGLRRRVGKGRILAVLEPRSNTMKLGVMKLALPDSLSGAEQIFAYGAANGRDALGWDL
AGALAPLGERAKTFSDLSALIEAVTAAARPGDHVLVMSNGGFGGVHQKLLDALAERAETAN
>Mature_461_residues
MHIHILGICGTFMGGIALIARQAGHRVTGCDANVYPPMSTQLEAQGIELIEGFDPEQLSIGADLYVIGNVISRGNALMEA
ILDRNLPYISGPQWLGDNVLRQKWVLAVAGTHGKTTTTSMLAWILQDAGYNPGFLVGGVPRNFGLSARLTESDFFVIEAD
EYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLAAIETQFHHLVRTVPGQGRLLVNGREDSLKRVLERGCWSEVEQ
FGAEGNLGWEVENLADDDSFDVVFQGQTQGRVRWALQGDHNRMNALAAIAAARHVGVPPAQAIESLARFENVKRRMEVRG
TVNDVTVYDDFAHHPTAIRTTIEGLRRRVGKGRILAVLEPRSNTMKLGVMKLALPDSLSGAEQIFAYGAANGRDALGWDL
AGALAPLGERAKTFSDLSALIEAVTAAARPGDHVLVMSNGGFGGVHQKLLDALAERAETAN

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=463, Percent_Identity=60.0431965442765, Blast_Score=562, Evalue=1e-161,
Organism=Escherichia coli, GI1786279, Length=358, Percent_Identity=30.1675977653631, Blast_Score=108, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 50243; Mature: 50243

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHILGICGTFMGGIALIARQAGHRVTGCDANVYPPMSTQLEAQGIELIEGFDPEQLSI
CEEEEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEC
GADLYVIGNVISRGNALMEAILDRNLPYISGPQWLGDNVLRQKWVLAVAGTHGKTTTTSM
CCCEEEEHHHHHCCHHHHHHHHHCCCCCCCCCCHHCHHHHHHEEEEEEECCCCCCHHHHH
LAWILQDAGYNPGFLVGGVPRNFGLSARLTESDFFVIEADEYDTAFFDKRSKFVHYRPRT
HHHHHHHCCCCCCEEECCCCCCCCCEEEEECCCEEEEECCCCCCHHHHCCCCEEEECCHH
AILNNLEFDHADIFPDLAAIETQFHHLVRTVPGQGRLLVNGREDSLKRVLERGCWSEVEQ
HHHCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCHHHHHH
FGAEGNLGWEVENLADDDSFDVVFQGQTQGRVRWALQGDHNRMNALAAIAAARHVGVPPA
CCCCCCCCEEECCCCCCCCEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCHH
QAIESLARFENVKRRMEVRGTVNDVTVYDDFAHHPTAIRTTIEGLRRRVGKGRILAVLEP
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECC
RSNTMKLGVMKLALPDSLSGAEQIFAYGAANGRDALGWDLAGALAPLGERAKTFSDLSAL
CCCCEEEEEEEEECCCCCCCHHHHHEECCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHH
IEAVTAAARPGDHVLVMSNGGFGGVHQKLLDALAERAETAN
HHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHIHILGICGTFMGGIALIARQAGHRVTGCDANVYPPMSTQLEAQGIELIEGFDPEQLSI
CEEEEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEC
GADLYVIGNVISRGNALMEAILDRNLPYISGPQWLGDNVLRQKWVLAVAGTHGKTTTTSM
CCCEEEEHHHHHCCHHHHHHHHHCCCCCCCCCCHHCHHHHHHEEEEEEECCCCCCHHHHH
LAWILQDAGYNPGFLVGGVPRNFGLSARLTESDFFVIEADEYDTAFFDKRSKFVHYRPRT
HHHHHHHCCCCCCEEECCCCCCCCCEEEEECCCEEEEECCCCCCHHHHCCCCEEEECCHH
AILNNLEFDHADIFPDLAAIETQFHHLVRTVPGQGRLLVNGREDSLKRVLERGCWSEVEQ
HHHCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCHHHHHH
FGAEGNLGWEVENLADDDSFDVVFQGQTQGRVRWALQGDHNRMNALAAIAAARHVGVPPA
CCCCCCCCEEECCCCCCCCEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCHH
QAIESLARFENVKRRMEVRGTVNDVTVYDDFAHHPTAIRTTIEGLRRRVGKGRILAVLEP
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECC
RSNTMKLGVMKLALPDSLSGAEQIFAYGAANGRDALGWDLAGALAPLGERAKTFSDLSAL
CCCCEEEEEEEEECCCCCCCHHHHHEECCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHH
IEAVTAAARPGDHVLVMSNGGFGGVHQKLLDALAERAETAN
HHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]