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The map label for this gene is araF [H]

Identifier: 187930065

GI number: 187930065

Start: 3124530

End: 3125534

Strand: Reverse

Name: araF [H]

Synonym: Rpic_2997

Alternate gene names: 187930065

Gene position: 3125534-3124530 (Counterclockwise)

Preceding gene: 187930066

Following gene: 187930064

Centisome position: 79.28

GC content: 63.38

Gene sequence:

>1005_bases
ATGCAACAACAACGACGCGGGACATTCAAGGCCATTGCGGCTGCCACGCTGTTTGCCATGGCCGGCGGCCTTTCAGTGTT
TGCCCATGCAGCCGATGACGTGAAGATCGGTTTCCTGGTCAAGCAGCCCGAAGAGCCGTGGTTCCAGGATGAGTGGAAGT
TCGCAGACCAGGCCGCCAAGGAGAAGGGCTTCAAGCTGGTAAAGATCGGCGTGCCCAGCGGCGGCGAAGTGCTGACGGCC
ATCGACAACCTGGGCGCGCAGCACGCGCAGGGTTTCGTGATCTGCGTGCCCGACGTGAAGCTCGGACCTGCAGTCGTGGC
CAAGGCCAAACAGAACAACCTCAAGCTGATGACGGTGGATGACCGTTTGGTGGATGGCGCCGGCAAGCCCATCGAAGCCG
TGCCGCACATGGGCATCTCGGCCACGAAGATCGGCGAGCAGGTGGGCGATGCCATCGCGCAGGAGATGAAGAAGCGCGGC
TGGAACCTGTCGGAGGTCGGCGCCATTCGCATCGCCTACGACCAGCTGCCGACCGCCAAGGAGCGCACCGACGGTGCAGT
CGCCGCGCTCAGCAAGGCGGGCTTCCCGGCCGCCAACGTGCTGACCAGCCCGCAGGCCAAGACGGATACCGAAGCCGCCT
TCAACGCCGCCAACATCACCCTGACCAAGAACCCGAAGTTCAAGCACTGGATTGCCTTCGGCCTGAATGACGAAGCCGTG
CTGGGTGCCGTGCGCGCAGCGGAAGGCCACGGCGTGAAGCCGGCGGACATCATCGGGGTAGGTATCGGCGGCAGCCAGTC
TGCGCTCAACGAGTTTGCCAAGCCCGAGAAGACCGGCTTCTTCGGCACCGTGCTGATTAGCCCGAAGCGCCACGGCTATG
AAACCTCGCTCAACATGTACGAGTGGGTCAAGAGCAACAAGGCGCCGGACCCGCTCATCCTGACCAGCGGCCGCCTGATG
ACGCGCGACAACGAAAAGGCCGTCCGCCAAGAGATGGGTTTGTGA

Upstream 100 bases:

>100_bases
GTCAAAACCTTGAAGGTGGCGTGAGCCCGGCGCACACGCCATCCCTCTTTCATCCAACGCAGAACAGATGCCGCAATGAC
GGTATCGCAAGGAGACAAAC

Downstream 100 bases:

>100_bases
TCTTGCGGATACCGTTGTTGAAGGAGACTTGCCATGTCGGCGTTTCTTGAGTTTCGTGGCATCAGCAAGGTGTTTCCGGG
TGTGCGGGCGCTGTCCGATG

Product: Monosaccharide-transporting ATPase

Products: ADP; phosphate; arabinose [Cytoplasm] [C]

Alternate protein names: ABP [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MQQQRRGTFKAIAAATLFAMAGGLSVFAHAADDVKIGFLVKQPEEPWFQDEWKFADQAAKEKGFKLVKIGVPSGGEVLTA
IDNLGAQHAQGFVICVPDVKLGPAVVAKAKQNNLKLMTVDDRLVDGAGKPIEAVPHMGISATKIGEQVGDAIAQEMKKRG
WNLSEVGAIRIAYDQLPTAKERTDGAVAALSKAGFPAANVLTSPQAKTDTEAAFNAANITLTKNPKFKHWIAFGLNDEAV
LGAVRAAEGHGVKPADIIGVGIGGSQSALNEFAKPEKTGFFGTVLISPKRHGYETSLNMYEWVKSNKAPDPLILTSGRLM
TRDNEKAVRQEMGL

Sequences:

>Translated_334_residues
MQQQRRGTFKAIAAATLFAMAGGLSVFAHAADDVKIGFLVKQPEEPWFQDEWKFADQAAKEKGFKLVKIGVPSGGEVLTA
IDNLGAQHAQGFVICVPDVKLGPAVVAKAKQNNLKLMTVDDRLVDGAGKPIEAVPHMGISATKIGEQVGDAIAQEMKKRG
WNLSEVGAIRIAYDQLPTAKERTDGAVAALSKAGFPAANVLTSPQAKTDTEAAFNAANITLTKNPKFKHWIAFGLNDEAV
LGAVRAAEGHGVKPADIIGVGIGGSQSALNEFAKPEKTGFFGTVLISPKRHGYETSLNMYEWVKSNKAPDPLILTSGRLM
TRDNEKAVRQEMGL
>Mature_334_residues
MQQQRRGTFKAIAAATLFAMAGGLSVFAHAADDVKIGFLVKQPEEPWFQDEWKFADQAAKEKGFKLVKIGVPSGGEVLTA
IDNLGAQHAQGFVICVPDVKLGPAVVAKAKQNNLKLMTVDDRLVDGAGKPIEAVPHMGISATKIGEQVGDAIAQEMKKRG
WNLSEVGAIRIAYDQLPTAKERTDGAVAALSKAGFPAANVLTSPQAKTDTEAAFNAANITLTKNPKFKHWIAFGLNDEAV
LGAVRAAEGHGVKPADIIGVGIGGSQSALNEFAKPEKTGFFGTVLISPKRHGYETSLNMYEWVKSNKAPDPLILTSGRLM
TRDNEKAVRQEMGL

Specific function: Involved in the high-affinity L-arabinose membrane transport system. Binds with high affinity to arabinose, but can also bind D-galactose (approximately 2-fold reduction) and D- fucose (approximately 40-fold reduction) [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788211, Length=326, Percent_Identity=47.5460122699387, Blast_Score=305, Evalue=3e-84,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35644; Mature: 35644

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQQRRGTFKAIAAATLFAMAGGLSVFAHAADDVKIGFLVKQPEEPWFQDEWKFADQAAK
CCCCCCCHHHHHHHHHHHHHHCCHHEEEECCCCEEEEEEEECCCCCCCCHHHHHHHHHHH
EKGFKLVKIGVPSGGEVLTAIDNLGAQHAQGFVICVPDVKLGPAVVAKAKQNNLKLMTVD
HCCCEEEEEECCCCCCEEHHHHHCCHHHHCEEEEEECCCCCCHHHEEECCCCCEEEEEEC
DRLVDGAGKPIEAVPHMGISATKIGEQVGDAIAQEMKKRGWNLSEVGAIRIAYDQLPTAK
CHHHCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCEEEEEECCCCCCH
ERTDGAVAALSKAGFPAANVLTSPQAKTDTEAAFNAANITLTKNPKFKHWIAFGLNDEAV
HHCCCHHHHHHHCCCCHHHHCCCCCCCCCHHHHCCEEEEEEECCCCEEEEEEECCCCHHH
LGAVRAAEGHGVKPADIIGVGIGGSQSALNEFAKPEKTGFFGTVLISPKRHGYETSLNMY
HHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHH
EWVKSNKAPDPLILTSGRLMTRDNEKAVRQEMGL
HHHHCCCCCCCEEEECCEEEECCCHHHHHHHHCC
>Mature Secondary Structure
MQQQRRGTFKAIAAATLFAMAGGLSVFAHAADDVKIGFLVKQPEEPWFQDEWKFADQAAK
CCCCCCCHHHHHHHHHHHHHHCCHHEEEECCCCEEEEEEEECCCCCCCCHHHHHHHHHHH
EKGFKLVKIGVPSGGEVLTAIDNLGAQHAQGFVICVPDVKLGPAVVAKAKQNNLKLMTVD
HCCCEEEEEECCCCCCEEHHHHHCCHHHHCEEEEEECCCCCCHHHEEECCCCCEEEEEEC
DRLVDGAGKPIEAVPHMGISATKIGEQVGDAIAQEMKKRGWNLSEVGAIRIAYDQLPTAK
CHHHCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCEEEEEECCCCCCH
ERTDGAVAALSKAGFPAANVLTSPQAKTDTEAAFNAANITLTKNPKFKHWIAFGLNDEAV
HHCCCHHHHHHHCCCCHHHHCCCCCCCCCHHHHCCEEEEEEECCCCEEEEEEECCCCHHH
LGAVRAAEGHGVKPADIIGVGIGGSQSALNEFAKPEKTGFFGTVLISPKRHGYETSLNMY
HHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHH
EWVKSNKAPDPLILTSGRLMTRDNEKAVRQEMGL
HHHHCCCCCCCEEEECCEEEECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arabinose [Periplasm]; H2O [C]

Specific reaction: ATP + arabinose [Periplasm] + H2O = ADP + phosphate + arabinose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2445996; 9097040; 9278503; 6885805; 326784; 9298646; 7031057; 326785; 2204627 [H]