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The map label for this gene is dgoD

Identifier: 187930058

GI number: 187930058

Start: 3117548

End: 3118696

Strand: Reverse

Name: dgoD

Synonym: Rpic_2990

Alternate gene names: 187930058

Gene position: 3118696-3117548 (Counterclockwise)

Preceding gene: 187930059

Following gene: 187930057

Centisome position: 79.1

GC content: 65.36

Gene sequence:

>1149_bases
ATGAAAATCACCCGCCTGACCACGTACCGCCTGCCGCCGCGCTGGATGTTCCTGAAAGTCGAGACCGACGAGGGCGTCAC
CGGCTGGGGCGAACCCGTTATCGAAGGCCGTGCGCGCACAGTGGAAGCGGCGGTGCACGAGCTGTCCGACTACCTCATCG
GCCAAGACCCGAGTCGCATCAACGACCTATGGCAGACCATGTACCGCGCGGGTTTCTACCGCGGCGGCCCGATCCTGATG
AGTGCCATTGCCGGCATCGACCAGGCGCTGTGGGACATCAAGGGCAAGGTGCTGGGCGTGCCGGTGTACGAACTGCTCGG
CGGCCTCGTGCGCGACAAGATGCGCACCTACAGCTGGGTCGGCGGCGATCGCCCGGCCGACGTGATTGCCGGCATGAAGG
CGCTGCAGGCCGGCGGCTTCGACCACTTCAAGCTCAACGGCTGCGAAGAGATGGGCATCATCGACACGTCCCGCGCCGTG
GATGCCGCGGTGGCGAGGGTTGCAGAGATTCGCTCGGCGTTCGGCAACACCGTCGAATTCGGGCTCGATTTTCACGGCCG
CGTTTCTGCGCCGATGGCCAAGGTGCTCATCAAGGAGCTGGAGCCGTACCGCCCGCTGTTTATCGAAGAACCGGTGCTTG
CCGAGCAGGCTGAAACCTACGCCCGCCTGGCCGCCCACACCCATCTGCCTATCGCTGCCGGCGAGCGCATGTTCTCGCGC
TTCGACTTCAAGCGCGTGCTGGAAGCGGGCGGGGTGTCGATCCTGCAGCCGGACCTGTCTCACGCCGGCGGCATTACTGA
ATGCGTGAAGATCGCCGCAATGGCCGAGGCCTACGATGTGGCGCTGGCACCGCATTGCCCGCTCGGCCCCATCGCGCTGG
CCGCCTGCCTGCACGTGGATTTCGTCAGCTGGAACGCCACGTTGCAGGAGCAGAGCATGGGCATCCACTACAACAAGGGC
GCCGAACTGCTCGATTACGTGCGAAACAAGGCCGATTTTGCTCTGGAAGGCGGGTATATCCGCCCGCCGCGCCTGCCCGG
CCTGGGAGTGGACATCGACGAGGCGCTGGTCATCGAACGCAGCAAGGAAGCCCCCGACTGGCGCAACCCGGTGTGGCGTC
ACGCCGACGGTTCCGTGGCGGAGTGGTAA

Upstream 100 bases:

>100_bases
GCCAGCCCGTCGAGCGCACCGCCGAACGCGCGCGCGCCTTCGTCACCGCCTACCGCAGCGCCCAATCCGACCGCACTTGA
ACGCACCATCGATTCGATCC

Downstream 100 bases:

>100_bases
GCCGGGCGGGGGAGGGGACGTCGTAGCACGTTTTACTGACCGCGATCACCCCAGCCGGGTCATCCCCGTCTGGTAAAATC
GCGGTTTTCCCGCCTATCTC

Product: galactonate dehydratase

Products: NA

Alternate protein names: GalD

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKITRLTTYRLPPRWMFLKVETDEGVTGWGEPVIEGRARTVEAAVHELSDYLIGQDPSRINDLWQTMYRAGFYRGGPILM
SAIAGIDQALWDIKGKVLGVPVYELLGGLVRDKMRTYSWVGGDRPADVIAGMKALQAGGFDHFKLNGCEEMGIIDTSRAV
DAAVARVAEIRSAFGNTVEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAETYARLAAHTHLPIAAGERMFSR
FDFKRVLEAGGVSILQPDLSHAGGITECVKIAAMAEAYDVALAPHCPLGPIALAACLHVDFVSWNATLQEQSMGIHYNKG
AELLDYVRNKADFALEGGYIRPPRLPGLGVDIDEALVIERSKEAPDWRNPVWRHADGSVAEW

Sequences:

>Translated_382_residues
MKITRLTTYRLPPRWMFLKVETDEGVTGWGEPVIEGRARTVEAAVHELSDYLIGQDPSRINDLWQTMYRAGFYRGGPILM
SAIAGIDQALWDIKGKVLGVPVYELLGGLVRDKMRTYSWVGGDRPADVIAGMKALQAGGFDHFKLNGCEEMGIIDTSRAV
DAAVARVAEIRSAFGNTVEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAETYARLAAHTHLPIAAGERMFSR
FDFKRVLEAGGVSILQPDLSHAGGITECVKIAAMAEAYDVALAPHCPLGPIALAACLHVDFVSWNATLQEQSMGIHYNKG
AELLDYVRNKADFALEGGYIRPPRLPGLGVDIDEALVIERSKEAPDWRNPVWRHADGSVAEW
>Mature_382_residues
MKITRLTTYRLPPRWMFLKVETDEGVTGWGEPVIEGRARTVEAAVHELSDYLIGQDPSRINDLWQTMYRAGFYRGGPILM
SAIAGIDQALWDIKGKVLGVPVYELLGGLVRDKMRTYSWVGGDRPADVIAGMKALQAGGFDHFKLNGCEEMGIIDTSRAV
DAAVARVAEIRSAFGNTVEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAETYARLAAHTHLPIAAGERMFSR
FDFKRVLEAGGVSILQPDLSHAGGITECVKIAAMAEAYDVALAPHCPLGPIALAACLHVDFVSWNATLQEQSMGIHYNKG
AELLDYVRNKADFALEGGYIRPPRLPGLGVDIDEALVIERSKEAPDWRNPVWRHADGSVAEW

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily

Homologues:

Organism=Homo sapiens, GI42544119, Length=415, Percent_Identity=23.1325301204819, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI186972148, Length=339, Percent_Identity=23.0088495575221, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI48994953, Length=382, Percent_Identity=83.7696335078534, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI1787864, Length=414, Percent_Identity=30.4347826086957, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI226510960, Length=275, Percent_Identity=30.9090909090909, Blast_Score=99, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DGOD_RALPJ (B2UCA8)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001900545.1
- ProteinModelPortal:   B2UCA8
- GeneID:   6288930
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_2990
- HOGENOM:   HBG705576
- OMA:   KADFALE
- ProtClustDB:   PRK14017
- HAMAP:   MF_01289
- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354
- PANTHER:   PTHR13794
- SMART:   SM00922

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N

EC number: =4.2.1.6

Molecular weight: Translated: 42099; Mature: 42099

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00908 MR_MLE_1; PS00909 MR_MLE_2

Important sites: ACT_SITE 185-185 ACT_SITE 285-285

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITRLTTYRLPPRWMFLKVETDEGVTGWGEPVIEGRARTVEAAVHELSDYLIGQDPSRI
CCCEEEEEEECCCCEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCHHHH
NDLWQTMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLGVPVYELLGGLVRDKMRTYSWV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHCC
GGDRPADVIAGMKALQAGGFDHFKLNGCEEMGIIDTSRAVDAAVARVAEIRSAFGNTVEF
CCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
GLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAETYARLAAHTHLPIAAGERMFSR
CCCCCCCCCHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
FDFKRVLEAGGVSILQPDLSHAGGITECVKIAAMAEAYDVALAPHCPLGPIALAACLHVD
HHHHHHHHHCCCEEECCCHHHCCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHH
FVSWNATLQEQSMGIHYNKGAELLDYVRNKADFALEGGYIRPPRLPGLGVDIDEALVIER
HCCCCCCHHHHCCCEEECCCHHHHHHHHCCCCEEEECCEECCCCCCCCCCCCCHHHEEEC
SKEAPDWRNPVWRHADGSVAEW
CCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKITRLTTYRLPPRWMFLKVETDEGVTGWGEPVIEGRARTVEAAVHELSDYLIGQDPSRI
CCCEEEEEEECCCCEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCHHHH
NDLWQTMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLGVPVYELLGGLVRDKMRTYSWV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHCC
GGDRPADVIAGMKALQAGGFDHFKLNGCEEMGIIDTSRAVDAAVARVAEIRSAFGNTVEF
CCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
GLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAETYARLAAHTHLPIAAGERMFSR
CCCCCCCCCHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
FDFKRVLEAGGVSILQPDLSHAGGITECVKIAAMAEAYDVALAPHCPLGPIALAACLHVD
HHHHHHHHHCCCEEECCCHHHCCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHH
FVSWNATLQEQSMGIHYNKGAELLDYVRNKADFALEGGYIRPPRLPGLGVDIDEALVIER
HCCCCCCHHHHCCCEEECCCHHHHHHHHCCCCEEEECCEECCCCCCCCCCCCCHHHEEEC
SKEAPDWRNPVWRHADGSVAEW
CCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA