| Definition | Ralstonia pickettii 12J chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010682 |
| Length | 3,942,557 |
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The map label for this gene is amiC [H]
Identifier: 187929884
GI number: 187929884
Start: 2923363
End: 2924883
Strand: Reverse
Name: amiC [H]
Synonym: Rpic_2815
Alternate gene names: 187929884
Gene position: 2924883-2923363 (Counterclockwise)
Preceding gene: 187929885
Following gene: 187929883
Centisome position: 74.19
GC content: 67.39
Gene sequence:
>1521_bases ATGCTGATCAAGCATCTGCCGACCGACCATCCGGATGATCCCAACCTGCCGAGCCAGGCCCGCCGCCAATGGCTCACGCG CATGGCGAAGGCCGGCGCAGGCACGGTCATCCTGAGCCTGGTGGGCCCGCAGATTGCGCGCGGCGCCAACATCGTCGCCG TGCGCGTGTGGCCCGCCGAGGACTACACACGCGTGACGATCGAATCCGACGTCGCGCTCAAGGCCACGCATCAGCTCATC AAGGATCCGGATCGCCTGATGGTCGATATCGACGGGCTCGAGCTGAACCCGACGTTGCGCGAGCTGGTGGCCAAGATCGC GCCGAACGATCCGTACATCCGCCAGGTGCGCGTGGGTCAGAACCGGCCGAGCGTCGTACGCCTGGTGTTCGACCTGAAGG AAAGCGTCAACCCGCAGGTGTTCACGCTGCTGCCGATTGCCGGCTATCGCAACCGGCTGGTGTTCGACCTGTACCCGACC AACCCGCCCGACCCGCTGATGCAGCTCGTGCAGGCCACCGAGGGCAAGCAGGAGCGCTTTGCCGCGTCGGGCGGCACGCC GATGCCGACACCGGATGACGATGACCCCATCGCTGCGCTGGCACGCCGCAGCAGCGGCGGCAGCCCAAACAAGCCGCCGA TCGAAGAGCCGCACCTCCCGGCGCTTGCCAACCGCACGCCGCCGCGCAAGGTGCCGCCGACGACCTCTTCGAATCCTCCG TCGACGCTCGCCAACGCGCCGAACACGCCGCCGCTGGAGATCATTCCCGAGCCACCCGTGCCGCGTGCACCCTCGAACGG CCCGCGCATGCGCCGCCTGCTCACGGTCGCCATCGACCCCGGCCATGGCGGCGAAGACCCGGGCGCCACGGGCGGTGCCG GCACGCACGAGAAAGACGTGGTGCTGTCGATCGCCCGCATGCTCAAGGCCAAGATCGACGCCCAGCCCAACATGCGCGCC ATGATGACGCGCGATTCCGACTACTTCGTCCCGCTGGGCGTGCGCGTGCAGAAGGCGCGCCGCGTGCAGGCGGATCTGTT TGTCTCCATCCATGCCGATGCGTTTCTTTCGCCGGAAGCGCGCGGGGCATCAGTGTTCGTGTTGTCCGATCGTGGCGCTT CGAGCGCGCAGGCCAAGTGGCTCGCCAACAAGGAAAACGCTGCCGACATGATCGGCGGCGCGAACCTCGGCTCGAAGGAC GCGCAGGTCACGCGCGTGCTGCTGGACCTGTCGACCACCGCGCAGATCACCGACAGCATGAGGGTCGGCAAGGCCGTGCT GGACCAGATCGGCGGCATCAACCGGCTGCACAAGGGGCAGGTGGAGCAGGCGGGCTTTGCGGTGCTCAAGGCGCCCGACA TTCCGTCGATCCTCGTCGAGACGGCCTTCATCTCCAACCCGGAAGAAGAAGCCAAGCTCAACGACCTGGGCCACCAGAAC CAGCTTGCCGACGCCATCCTGCGCGGCATTCGCGCCTACTTTGCCAAGAACCCGCCGTTGTCGCGCAACCCGGGCGTCTG A
Upstream 100 bases:
>100_bases CCTACGATGACGGCGTTGAGCATGCACCGCGCGCAGCACGTCTGAGCGCACGCACGCAGACCGGCCTTCAACTTCTACAG TCGTTGCAACACCTGTTCCC
Downstream 100 bases:
>100_bases CGCATGACGACGCTTGTGCTACGCGAAGGCGGTGCGTCTGACGCCAGCACCATCGCCGACATCCACACGCGAAGCTGGCA GACCGCGTATCGCGGGCTCG
Product: N-acetylmuramoyl-L-alanine amidase
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 506; Mature: 506
Protein sequence:
>506_residues MLIKHLPTDHPDDPNLPSQARRQWLTRMAKAGAGTVILSLVGPQIARGANIVAVRVWPAEDYTRVTIESDVALKATHQLI KDPDRLMVDIDGLELNPTLRELVAKIAPNDPYIRQVRVGQNRPSVVRLVFDLKESVNPQVFTLLPIAGYRNRLVFDLYPT NPPDPLMQLVQATEGKQERFAASGGTPMPTPDDDDPIAALARRSSGGSPNKPPIEEPHLPALANRTPPRKVPPTTSSNPP STLANAPNTPPLEIIPEPPVPRAPSNGPRMRRLLTVAIDPGHGGEDPGATGGAGTHEKDVVLSIARMLKAKIDAQPNMRA MMTRDSDYFVPLGVRVQKARRVQADLFVSIHADAFLSPEARGASVFVLSDRGASSAQAKWLANKENAADMIGGANLGSKD AQVTRVLLDLSTTAQITDSMRVGKAVLDQIGGINRLHKGQVEQAGFAVLKAPDIPSILVETAFISNPEEEAKLNDLGHQN QLADAILRGIRAYFAKNPPLSRNPGV
Sequences:
>Translated_506_residues MLIKHLPTDHPDDPNLPSQARRQWLTRMAKAGAGTVILSLVGPQIARGANIVAVRVWPAEDYTRVTIESDVALKATHQLI KDPDRLMVDIDGLELNPTLRELVAKIAPNDPYIRQVRVGQNRPSVVRLVFDLKESVNPQVFTLLPIAGYRNRLVFDLYPT NPPDPLMQLVQATEGKQERFAASGGTPMPTPDDDDPIAALARRSSGGSPNKPPIEEPHLPALANRTPPRKVPPTTSSNPP STLANAPNTPPLEIIPEPPVPRAPSNGPRMRRLLTVAIDPGHGGEDPGATGGAGTHEKDVVLSIARMLKAKIDAQPNMRA MMTRDSDYFVPLGVRVQKARRVQADLFVSIHADAFLSPEARGASVFVLSDRGASSAQAKWLANKENAADMIGGANLGSKD AQVTRVLLDLSTTAQITDSMRVGKAVLDQIGGINRLHKGQVEQAGFAVLKAPDIPSILVETAFISNPEEEAKLNDLGHQN QLADAILRGIRAYFAKNPPLSRNPGV >Mature_506_residues MLIKHLPTDHPDDPNLPSQARRQWLTRMAKAGAGTVILSLVGPQIARGANIVAVRVWPAEDYTRVTIESDVALKATHQLI KDPDRLMVDIDGLELNPTLRELVAKIAPNDPYIRQVRVGQNRPSVVRLVFDLKESVNPQVFTLLPIAGYRNRLVFDLYPT NPPDPLMQLVQATEGKQERFAASGGTPMPTPDDDDPIAALARRSSGGSPNKPPIEEPHLPALANRTPPRKVPPTTSSNPP STLANAPNTPPLEIIPEPPVPRAPSNGPRMRRLLTVAIDPGHGGEDPGATGGAGTHEKDVVLSIARMLKAKIDAQPNMRA MMTRDSDYFVPLGVRVQKARRVQADLFVSIHADAFLSPEARGASVFVLSDRGASSAQAKWLANKENAADMIGGANLGSKD AQVTRVLLDLSTTAQITDSMRVGKAVLDQIGGINRLHKGQVEQAGFAVLKAPDIPSILVETAFISNPEEEAKLNDLGHQN QLADAILRGIRAYFAKNPPLSRNPGV
Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]
COG id: COG0860
COG function: function code M; N-acetylmuramoyl-L-alanine amidase
Gene ontology:
Cell location: Periplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]
Homologues:
Organism=Escherichia coli, GI87082163, Length=472, Percent_Identity=44.2796610169491, Blast_Score=368, Evalue=1e-103, Organism=Escherichia coli, GI1788776, Length=234, Percent_Identity=46.1538461538462, Blast_Score=202, Evalue=4e-53, Organism=Escherichia coli, GI1790611, Length=249, Percent_Identity=42.570281124498, Blast_Score=184, Evalue=8e-48,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR021731 - InterPro: IPR002508 - InterPro: IPR006311 [H]
Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]
EC number: =3.5.1.28 [H]
Molecular weight: Translated: 54583; Mature: 54583
Theoretical pI: Translated: 9.66; Mature: 9.66
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.0 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 2.4 %Cys+Met (Translated Protein) 0.0 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 2.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MLIKHLPTDHPDDPNLPSQARRQWLTRMAKAGAGTVILSLVGPQIARGANIVAVRVWPAE CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHCCCCEEEEEEECCC DYTRVTIESDVALKATHQLIKDPDRLMVDIDGLELNPTLRELVAKIAPNDPYIRQVRVGQ CCEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECC NRPSVVRLVFDLKESVNPQVFTLLPIAGYRNRLVFDLYPTNPPDPLMQLVQATEGKQERF CCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHCCCHHHHH AASGGTPMPTPDDDDPIAALARRSSGGSPNKPPIEEPHLPALANRTPPRKVPPTTSSNPP HCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCC STLANAPNTPPLEIIPEPPVPRAPSNGPRMRRLLTVAIDPGHGGEDPGATGGAGTHEKDV HHHCCCCCCCCCEEECCCCCCCCCCCCHHEEEEEEEEECCCCCCCCCCCCCCCCCCCHHH VLSIARMLKAKIDAQPNMRAMMTRDSDYFVPLGVRVQKARRVQADLFVSIHADAFLSPEA HHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCHHHHHHCCEEEEEEEECCEEECCCC RGASVFVLSDRGASSAQAKWLANKENAADMIGGANLGSKDAQVTRVLLDLSTTAQITDSM CCCEEEEEECCCCCCHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHH RVGKAVLDQIGGINRLHKGQVEQAGFAVLKAPDIPSILVETAFISNPEEEAKLNDLGHQN HHHHHHHHHHCCCCHHHCCCHHHCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHCCCHH QLADAILRGIRAYFAKNPPLSRNPGV HHHHHHHHHHHHHHHCCCCCCCCCCC >Mature Secondary Structure MLIKHLPTDHPDDPNLPSQARRQWLTRMAKAGAGTVILSLVGPQIARGANIVAVRVWPAE CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHCCCCEEEEEEECCC DYTRVTIESDVALKATHQLIKDPDRLMVDIDGLELNPTLRELVAKIAPNDPYIRQVRVGQ CCEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECC NRPSVVRLVFDLKESVNPQVFTLLPIAGYRNRLVFDLYPTNPPDPLMQLVQATEGKQERF CCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHCCCHHHHH AASGGTPMPTPDDDDPIAALARRSSGGSPNKPPIEEPHLPALANRTPPRKVPPTTSSNPP HCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCC STLANAPNTPPLEIIPEPPVPRAPSNGPRMRRLLTVAIDPGHGGEDPGATGGAGTHEKDV HHHCCCCCCCCCEEECCCCCCCCCCCCHHEEEEEEEEECCCCCCCCCCCCCCCCCCCHHH VLSIARMLKAKIDAQPNMRAMMTRDSDYFVPLGVRVQKARRVQADLFVSIHADAFLSPEA HHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCHHHHHHCCEEEEEEEECCEEECCCC RGASVFVLSDRGASSAQAKWLANKENAADMIGGANLGSKDAQVTRVLLDLSTTAQITDSM CCCEEEEEECCCCCCHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHH RVGKAVLDQIGGINRLHKGQVEQAGFAVLKAPDIPSILVETAFISNPEEEAKLNDLGHQN HHHHHHHHHHCCCCHHHCCCHHHCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHCCCHH QLADAILRGIRAYFAKNPPLSRNPGV HHHHHHHHHHHHHHHCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 12471157 [H]