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The map label for this gene is glnH [H]

Identifier: 187929867

GI number: 187929867

Start: 2908008

End: 2908772

Strand: Reverse

Name: glnH [H]

Synonym: Rpic_2798

Alternate gene names: 187929867

Gene position: 2908772-2908008 (Counterclockwise)

Preceding gene: 187929868

Following gene: 187929866

Centisome position: 73.78

GC content: 63.66

Gene sequence:

>765_bases
ATGGGGAATCGTCGTCAGTTCATGCATTCGATGCTGGCTTGTGCCGTGGCGTTGTCCGCTGCGGCGTCCTTCGCAACCGC
TGCGCACGCTGCTGGGGAGCCGTTGCGGGTGGCTACCGACGCCACGTTCGCGCCGATGGAGTTTGTCGAAAACGGCAAGC
GCACCGGCTTTGATGTGGACCTGATCGATGCGCTGGCCCGCACCCTGGGCCGCCCGGTCGAGTGGACCGACATCGACTTC
AAGGGCCTCGTGCCGGGCCTGATCTCGCGCCGCTTCGACGTGGCCATTTCGGGCATCTACATCACGGAAGAGCGCAAGAA
GGTGGTGGACTTCACGGTGCCGTACTACCACGGCGGCCTGGTCGCGCTGGTCAAGCAAGGCAACACGGCCATCAAGACGC
CGGCGGATCTGAACGGCAAGAAGGTCAGCGTGCAGGTGGGCACCAAATCGGTCAACTACCTGCGCGAGAACTACCCGAAG
GTCGAACGCGTGGAAGTCGAGAAGAACAACGAGATGTTCAACCTCGTCGAGATCGGCCGGGCCGATGCCGCTGTCACAGG
CCGCCCCGCCGCCGCGTACTACGTGAAGGCGCGTCCGGGCCTGCGCCTGGTTGAGCCGGCACTCACCACCGAGGAATACG
GCATCGCCGTGCGTCGCGATCAGCCCGAATTGACCAAGGCACTGAACGCCGCGCTGGAGAAGATCAAGGCGGACGGCACG
TACGACCAGATCGTCAAGAAGTGGTTTGGCACCACCGCGAAGTAA

Upstream 100 bases:

>100_bases
CGGCTGCGTCCATTTCTGCGCGATTGCAGGACCTCTGAATTTTCGATTTTTCCGCTTCGTTTTTTTCTCACCCCACATGC
ATCACACAAGGAGACCACTC

Downstream 100 bases:

>100_bases
CGACCGGACACGCTGATGGAACTGGATTTTTCTCCCGTCTGGGCCGGATGGCACGACATCCTGCGCGGTGCGATCGTCAC
CGTTGAGGTGACGGCCGCCG

Product: family 3 extracellular solute-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: GlnBP [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MGNRRQFMHSMLACAVALSAAASFATAAHAAGEPLRVATDATFAPMEFVENGKRTGFDVDLIDALARTLGRPVEWTDIDF
KGLVPGLISRRFDVAISGIYITEERKKVVDFTVPYYHGGLVALVKQGNTAIKTPADLNGKKVSVQVGTKSVNYLRENYPK
VERVEVEKNNEMFNLVEIGRADAAVTGRPAAAYYVKARPGLRLVEPALTTEEYGIAVRRDQPELTKALNAALEKIKADGT
YDQIVKKWFGTTAK

Sequences:

>Translated_254_residues
MGNRRQFMHSMLACAVALSAAASFATAAHAAGEPLRVATDATFAPMEFVENGKRTGFDVDLIDALARTLGRPVEWTDIDF
KGLVPGLISRRFDVAISGIYITEERKKVVDFTVPYYHGGLVALVKQGNTAIKTPADLNGKKVSVQVGTKSVNYLRENYPK
VERVEVEKNNEMFNLVEIGRADAAVTGRPAAAYYVKARPGLRLVEPALTTEEYGIAVRRDQPELTKALNAALEKIKADGT
YDQIVKKWFGTTAK
>Mature_253_residues
GNRRQFMHSMLACAVALSAAASFATAAHAAGEPLRVATDATFAPMEFVENGKRTGFDVDLIDALARTLGRPVEWTDIDFK
GLVPGLISRRFDVAISGIYITEERKKVVDFTVPYYHGGLVALVKQGNTAIKTPADLNGKKVSVQVGTKSVNYLRENYPKV
ERVEVEKNNEMFNLVEIGRADAAVTGRPAAAYYVKARPGLRLVEPALTTEEYGIAVRRDQPELTKALNAALEKIKADGTY
DQIVKKWFGTTAK

Specific function: Involved in a glutamine-transport system glnHPQ [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=249, Percent_Identity=38.1526104417671, Blast_Score=175, Evalue=2e-45,
Organism=Escherichia coli, GI1787085, Length=234, Percent_Identity=36.3247863247863, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1788228, Length=259, Percent_Identity=35.5212355212355, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI1787088, Length=235, Percent_Identity=36.1702127659575, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI1788649, Length=249, Percent_Identity=29.718875502008, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1788648, Length=226, Percent_Identity=29.2035398230088, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27808; Mature: 27677

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNRRQFMHSMLACAVALSAAASFATAAHAAGEPLRVATDATFAPMEFVENGKRTGFDVD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCCHH
LIDALARTLGRPVEWTDIDFKGLVPGLISRRFDVAISGIYITEERKKVVDFTVPYYHGGL
HHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHEEEEEEEEEECCCCEEEEEECCCCCCCE
VALVKQGNTAIKTPADLNGKKVSVQVGTKSVNYLRENYPKVERVEVEKNNEMFNLVEIGR
EEEEECCCCEECCCCCCCCCEEEEEECCHHHHHHHHCCCCCEEEEEECCCCEEEHEECCC
ADAAVTGRPAAAYYVKARPGLRLVEPALTTEEYGIAVRRDQPELTKALNAALEKIKADGT
CCCEECCCCCEEEEEECCCCCEEECCCCCHHHHCEEEECCCHHHHHHHHHHHHHHCCCCC
YDQIVKKWFGTTAK
HHHHHHHHCCCCCC
>Mature Secondary Structure 
GNRRQFMHSMLACAVALSAAASFATAAHAAGEPLRVATDATFAPMEFVENGKRTGFDVD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCCHH
LIDALARTLGRPVEWTDIDFKGLVPGLISRRFDVAISGIYITEERKKVVDFTVPYYHGGL
HHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHEEEEEEEEEECCCCEEEEEECCCCCCCE
VALVKQGNTAIKTPADLNGKKVSVQVGTKSVNYLRENYPKVERVEVEKNNEMFNLVEIGR
EEEEECCCCEECCCCCCCCCEEEEEECCHHHHHHHHCCCCCEEEEEECCCCEEEHEECCC
ADAAVTGRPAAAYYVKARPGLRLVEPALTTEEYGIAVRRDQPELTKALNAALEKIKADGT
CCCEECCCCCEEEEEECCCCCEEECCCCCHHHHCEEEECCCHHHHHHHHHHHHHHCCCCC
YDQIVKKWFGTTAK
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]