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The map label for this gene is ispH [H]

Identifier: 187929780

GI number: 187929780

Start: 2824396

End: 2825379

Strand: Reverse

Name: ispH [H]

Synonym: Rpic_2710

Alternate gene names: 187929780

Gene position: 2825379-2824396 (Counterclockwise)

Preceding gene: 187929781

Following gene: 187929779

Centisome position: 71.66

GC content: 63.52

Gene sequence:

>984_bases
ATGAGCAACGCTGTGAACGCCCCGCTCGATCCCATTGATCCTGTCACCCAGGCCGACGCCGAGGTCTTGCTGGCGCAGCC
GCGCGGCTTCTGCGCCGGCGTGGATCGTGCCATCGAGATCGTCGAGCGCGCGCTGGAGTTGTTTGGCGCGCCGATTTACG
TTCGTCACGAGATCGTGCACAACGCTTATGTGGTTGGCGATCTGCGCAACAAGGGCGCGGTGTTCGTGCAGGAACTCGAC
GATGTGCCAGTGGGTGGCACAGTCATCTTCAGTGCGCACGGCGTGTCACGGGCGGTGCGTGAGGCGGCGGAAGCGCGCGG
CCTGCGCGTGTTCGATGCGACCTGCCCGCTGGTGACCAAGGTGCACGTGGAAGTCTCGAAGATGCGTGCGCAGGGTTTCG
AGATCATCATGATCGGGCACAAGGGCCATCCCGAGGTGGAAGGCACCATGGGGCAGGCCAACGACGGCATGCTGCTGGTT
GAGTCCGTCGATGACGTTGCGCATCTCGAGGTGAAAGACTCGACGCGTCTGGCCTACGTGACGCAGACCACGCTGTCGGT
GGATGAGACCCAGGAGATCGTGGCGGCCATCAAAGCGCGCTTTCCGGCCGTGCACGAGCCCAAGAAGCAGGACATCTGCT
ACGCGACGCAGAATCGCCAGGACGCCGTGAAGTTCATGGCGCCGCAGGTGGAAGTGGTGATCGTGGTCGGCAGCCCGAAC
AGCTCGAACTCGAACCGCCTGCGTGAACTGGCCGAAAAGCTGGGCGTACCTGCCTATATGGTGGATACACCTGAACAGGT
GCGCCCCGAATGGGTGGCTGGCAAGCGCCGCGTGGGCCTGACCGCGGGGGCGTCGGCACCCGAAGAGCTGGCGCAATCGA
TTGTCGATCGGCTCAAGGCGCTGGGCGCACGCACCGTGCGCCCGCTCGACGGTATTCAGGAAAACATGTCTTTCCCCCTT
CCGAGGGGGCTTCAAATCAATTGA

Upstream 100 bases:

>100_bases
CTCAAGGAAGTGGGCGAGACCTCGGCATTGTTTGACTTCAACCACCCGCTGGCCGGTCAGACGATCGTGTTTGAAGTCCA
GCTGATCGGCATCCTCTGAT

Downstream 100 bases:

>100_bases
ATCCCTTTATGTAAATGCCCACTTAAAAGCGCACAAAAACGGTGCGCCCTAATGCGGTGCATAAAGCGCTTTTGAGGAAT
CGACGGCCACTTCTCCGGGA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSNAVNAPLDPIDPVTQADAEVLLAQPRGFCAGVDRAIEIVERALELFGAPIYVRHEIVHNAYVVGDLRNKGAVFVQELD
DVPVGGTVIFSAHGVSRAVREAAEARGLRVFDATCPLVTKVHVEVSKMRAQGFEIIMIGHKGHPEVEGTMGQANDGMLLV
ESVDDVAHLEVKDSTRLAYVTQTTLSVDETQEIVAAIKARFPAVHEPKKQDICYATQNRQDAVKFMAPQVEVVIVVGSPN
SSNSNRLRELAEKLGVPAYMVDTPEQVRPEWVAGKRRVGLTAGASAPEELAQSIVDRLKALGARTVRPLDGIQENMSFPL
PRGLQIN

Sequences:

>Translated_327_residues
MSNAVNAPLDPIDPVTQADAEVLLAQPRGFCAGVDRAIEIVERALELFGAPIYVRHEIVHNAYVVGDLRNKGAVFVQELD
DVPVGGTVIFSAHGVSRAVREAAEARGLRVFDATCPLVTKVHVEVSKMRAQGFEIIMIGHKGHPEVEGTMGQANDGMLLV
ESVDDVAHLEVKDSTRLAYVTQTTLSVDETQEIVAAIKARFPAVHEPKKQDICYATQNRQDAVKFMAPQVEVVIVVGSPN
SSNSNRLRELAEKLGVPAYMVDTPEQVRPEWVAGKRRVGLTAGASAPEELAQSIVDRLKALGARTVRPLDGIQENMSFPL
PRGLQIN
>Mature_326_residues
SNAVNAPLDPIDPVTQADAEVLLAQPRGFCAGVDRAIEIVERALELFGAPIYVRHEIVHNAYVVGDLRNKGAVFVQELDD
VPVGGTVIFSAHGVSRAVREAAEARGLRVFDATCPLVTKVHVEVSKMRAQGFEIIMIGHKGHPEVEGTMGQANDGMLLVE
SVDDVAHLEVKDSTRLAYVTQTTLSVDETQEIVAAIKARFPAVHEPKKQDICYATQNRQDAVKFMAPQVEVVIVVGSPNS
SNSNRLRELAEKLGVPAYMVDTPEQVRPEWVAGKRRVGLTAGASAPEELAQSIVDRLKALGARTVRPLDGIQENMSFPLP
RGLQIN

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=310, Percent_Identity=57.4193548387097, Blast_Score=373, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 35463; Mature: 35332

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNAVNAPLDPIDPVTQADAEVLLAQPRGFCAGVDRAIEIVERALELFGAPIYVRHEIVH
CCCCCCCCCCCCCCCHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHH
NAYVVGDLRNKGAVFVQELDDVPVGGTVIFSAHGVSRAVREAAEARGLRVFDATCPLVTK
CEEEEEECCCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCEEEE
VHVEVSKMRAQGFEIIMIGHKGHPEVEGTMGQANDGMLLVESVDDVAHLEVKDSTRLAYV
EHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCEEEEE
TQTTLSVDETQEIVAAIKARFPAVHEPKKQDICYATQNRQDAVKFMAPQVEVVIVVGSPN
EEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHCCCCEEEEEEEECCC
SSNSNRLRELAEKLGVPAYMVDTPEQVRPEWVAGKRRVGLTAGASAPEELAQSIVDRLKA
CCCHHHHHHHHHHHCCCEEEECCHHHHCCHHHCCCCEECEECCCCCHHHHHHHHHHHHHH
LGARTVRPLDGIQENMSFPLPRGLQIN
CCCCCCCCHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SNAVNAPLDPIDPVTQADAEVLLAQPRGFCAGVDRAIEIVERALELFGAPIYVRHEIVH
CCCCCCCCCCCCCCHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHH
NAYVVGDLRNKGAVFVQELDDVPVGGTVIFSAHGVSRAVREAAEARGLRVFDATCPLVTK
CEEEEEECCCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCEEEE
VHVEVSKMRAQGFEIIMIGHKGHPEVEGTMGQANDGMLLVESVDDVAHLEVKDSTRLAYV
EHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCEEEEE
TQTTLSVDETQEIVAAIKARFPAVHEPKKQDICYATQNRQDAVKFMAPQVEVVIVVGSPN
EEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHCCCCEEEEEEEECCC
SSNSNRLRELAEKLGVPAYMVDTPEQVRPEWVAGKRRVGLTAGASAPEELAQSIVDRLKA
CCCHHHHHHHHHHHCCCEEEECCHHHHCCHHHCCCCEECEECCCCCHHHHHHHHHHHHHH
LGARTVRPLDGIQENMSFPLPRGLQIN
CCCCCCCCHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]