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The map label for this gene is braD [H]

Identifier: 187929778

GI number: 187929778

Start: 2821591

End: 2822526

Strand: Reverse

Name: braD [H]

Synonym: Rpic_2708

Alternate gene names: 187929778

Gene position: 2822526-2821591 (Counterclockwise)

Preceding gene: 187929779

Following gene: 187929777

Centisome position: 71.59

GC content: 60.79

Gene sequence:

>936_bases
ATGGATATCTTTATCCAGCAGATCGTGAACGGTCTGGTGCTCGGCAGCATTTACGCGCTGATCGCACTTGGCTACACCAT
GGTCTACGGTATTCTCGGCATCATCAACTTCGCCCACGGCGATGTTCTGATGGTCGGTGCAATGTCTGCCCTGACCGCCA
TCAACTTCCTCCAGAAGTACTTCCCCAATCTGCCTGACTGGCTCACGCTGGTGTTCGCGTTGCTGTTTGCCATGCCGGTC
TGCGCCATCGTGGCCTACACCATCGAACGTGTCGCTTACCGGCCGCTGCGCAACGCGCCGCGCCTGGCGCCGCTGATCAC
CGCCATCGGCGTGTCGATCGTGCTGCAGACACTCGCGATGATGATCTGGTCGCGCAACCCGCTCACCTTCCCGCAACTGC
TGCCTTCGAGCCCGATTGATATCGGCTCCACGGGCGCAACCATTACGGGCAAGGAAATCGTCATCATCCTGGTGTCGATC
CTGGTGATGGTCGGGCTGATCCTGCTGGTCAACCGCACCAAGCTCGGCCGCGCGATGCGCGCGACCGCCGAGAACCAGCG
TGTGGCAGGCCTGATGGGCGTCAACCCGAACTTCGTGATCTCTGCCACCTTCATGATTGGTGCTGCCATGGCTGCGGTTG
CAGGCGTGATGATGGCGACCAACTACGGCAATGCTCACTTCTACATGGGCTTCATCCCCGGCCTGAAGGCGTTTACCGCC
GCGGTGCTGGGCGGCATCGGCAACCTGGCAGGCGCCATGGTTGGCGGCATGCTGCTGGGCCTGATCGAAGCTCTGGGTGC
CGGTTATATCGGTGATCTGACAGGCGGTGTGTTCGGTTCGAACTACCAGGATGTGTTCGCATTCATCGTGCTGATTGGCG
TGCTGCTGTTCCGCCCGTCCGGCATCATGGGCGAACGCGTTGCGGACCGCGCATAA

Upstream 100 bases:

>100_bases
TTACCAGCAACCTCACCTTACCCATCCGATTGATCGCATACCTTGCGGGCACGTCATCCCGTCGCCTACAACAAAGCAAG
GCATAGCAGGAGCTGTTCTC

Downstream 100 bases:

>100_bases
GAAGGGGAGCGCCATGACAGAAAATTTCAAAGCGCCGATGAAGACGCGCGCCGCGTTGTACGGCTTCCTGATCCTTGCGA
TCTTCGCGCCGTTCATCGTG

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MDIFIQQIVNGLVLGSIYALIALGYTMVYGILGIINFAHGDVLMVGAMSALTAINFLQKYFPNLPDWLTLVFALLFAMPV
CAIVAYTIERVAYRPLRNAPRLAPLITAIGVSIVLQTLAMMIWSRNPLTFPQLLPSSPIDIGSTGATITGKEIVIILVSI
LVMVGLILLVNRTKLGRAMRATAENQRVAGLMGVNPNFVISATFMIGAAMAAVAGVMMATNYGNAHFYMGFIPGLKAFTA
AVLGGIGNLAGAMVGGMLLGLIEALGAGYIGDLTGGVFGSNYQDVFAFIVLIGVLLFRPSGIMGERVADRA

Sequences:

>Translated_311_residues
MDIFIQQIVNGLVLGSIYALIALGYTMVYGILGIINFAHGDVLMVGAMSALTAINFLQKYFPNLPDWLTLVFALLFAMPV
CAIVAYTIERVAYRPLRNAPRLAPLITAIGVSIVLQTLAMMIWSRNPLTFPQLLPSSPIDIGSTGATITGKEIVIILVSI
LVMVGLILLVNRTKLGRAMRATAENQRVAGLMGVNPNFVISATFMIGAAMAAVAGVMMATNYGNAHFYMGFIPGLKAFTA
AVLGGIGNLAGAMVGGMLLGLIEALGAGYIGDLTGGVFGSNYQDVFAFIVLIGVLLFRPSGIMGERVADRA
>Mature_311_residues
MDIFIQQIVNGLVLGSIYALIALGYTMVYGILGIINFAHGDVLMVGAMSALTAINFLQKYFPNLPDWLTLVFALLFAMPV
CAIVAYTIERVAYRPLRNAPRLAPLITAIGVSIVLQTLAMMIWSRNPLTFPQLLPSSPIDIGSTGATITGKEIVIILVSI
LVMVGLILLVNRTKLGRAMRATAENQRVAGLMGVNPNFVISATFMIGAAMAAVAGVMMATNYGNAHFYMGFIPGLKAFTA
AVLGGIGNLAGAMVGGMLLGLIEALGAGYIGDLTGGVFGSNYQDVFAFIVLIGVLLFRPSGIMGERVADRA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=310, Percent_Identity=44.1935483870968, Blast_Score=221, Evalue=7e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 32852; Mature: 32852

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIFIQQIVNGLVLGSIYALIALGYTMVYGILGIINFAHGDVLMVGAMSALTAINFLQKY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
FPNLPDWLTLVFALLFAMPVCAIVAYTIERVAYRPLRNAPRLAPLITAIGVSIVLQTLAM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
MIWSRNPLTFPQLLPSSPIDIGSTGATITGKEIVIILVSILVMVGLILLVNRTKLGRAMR
HHHCCCCCCCHHHCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATAENQRVAGLMGVNPNFVISATFMIGAAMAAVAGVMMATNYGNAHFYMGFIPGLKAFTA
HHHCCCHHHEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHH
AVLGGIGNLAGAMVGGMLLGLIEALGAGYIGDLTGGVFGSNYQDVFAFIVLIGVLLFRPS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC
GIMGERVADRA
CCHHHHHHCCC
>Mature Secondary Structure
MDIFIQQIVNGLVLGSIYALIALGYTMVYGILGIINFAHGDVLMVGAMSALTAINFLQKY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
FPNLPDWLTLVFALLFAMPVCAIVAYTIERVAYRPLRNAPRLAPLITAIGVSIVLQTLAM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
MIWSRNPLTFPQLLPSSPIDIGSTGATITGKEIVIILVSILVMVGLILLVNRTKLGRAMR
HHHCCCCCCCHHHCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATAENQRVAGLMGVNPNFVISATFMIGAAMAAVAGVMMATNYGNAHFYMGFIPGLKAFTA
HHHCCCHHHEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHH
AVLGGIGNLAGAMVGGMLLGLIEALGAGYIGDLTGGVFGSNYQDVFAFIVLIGVLLFRPS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC
GIMGERVADRA
CCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]