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The map label for this gene is braE [H]

Identifier: 187929777

GI number: 187929777

Start: 2820450

End: 2821577

Strand: Reverse

Name: braE [H]

Synonym: Rpic_2707

Alternate gene names: 187929777

Gene position: 2821577-2820450 (Counterclockwise)

Preceding gene: 187929778

Following gene: 187929776

Centisome position: 71.57

GC content: 60.9

Gene sequence:

>1128_bases
ATGACAGAAAATTTCAAAGCGCCGATGAAGACGCGCGCCGCGTTGTACGGCTTCCTGATCCTTGCGATCTTCGCGCCGTT
CATCGTGGGCGCCGCAGGTGGCAACTACTGGGTGCGCGTGCTGGACTTCGCACTGCTGTACATCATGCTGGCCCTGGGCT
TGAACATCGTGGTGGGCTTTGCCGGCCTGCTCGACCTGGGCTACATCGCGTTCTATGCGGTGGGTGCCTACATGATGGCG
TTGCTGGGTTCGCCGCACCTGTCCAATCAGTTCGAGTGGATCCACAACCTGTTCCCCAACGGGCTGCACCTGATCGTATG
GTGGGTGATTCCGCTCGGTGCCGGGCTGGCGGCGCTGTTCGGCATTCTGCTGGGCACGCCGGTGCTGAAGCTGCGCGGTG
ACTACCTCGCCATCGTGACGCTGGGCTTTGGTGAAATCATCCGGATCTTCATGAACAACCTCGACCGTCCGGTGAACATC
ACCAACGGCCCGAAGGGCATCAACCTGATCGAGCCGGTCAAGATCTTCGGGTTCGACTTCTCGAAGCGCCATGATTTCTT
CGGCATCCGCTTCGACTCGGTCTACATGTACTACTACCTGTTCGTGATCCTGGCTGCGATCATCATCATCGTGTGCCTGC
GGTTGCAGAACTCGCGTATCGGTCGTGCATGGGTGGCGCTGCGTGAAGATGAAATCGCCGCCAAGGCGATGGGCATCAAC
ACGCGCAACATCAAGCTGCTCGCCTTTGCGATGGGTGCGTCGTTCGGTGGCGTGTCGGGTGCGATGTTCGCGTCGTTCCA
GGGCTTCGTGTCGCCGGAATCGTTCGTGCTGTGGGAGTCGATCTACATCCTGGCGATCGTGGTGCTGGGCGGCATGGGCC
ATATCCCGGGCGTGATCCTGGGCGGCATTCTGCTGGTCGGTTTCCAGGAACTGCTGCGTGCGCTGGCCGAGCCGGTGCAG
AACAAGCTGTTCGGCCATGTGATCGTGGAAGCGGAAGTGCTTCGTCAGCTGCTGTTCGGCCTGGCGATGGTGGTGGTGAT
GCTGTATCGCCCGTCGGGCCTGTGGCCCTCGCCGCGCAAGGAAGACCGTCCGCAAAAGCAGCGGGCTGGTGGACTGTCCC
GTATCTGA

Upstream 100 bases:

>100_bases
GATGTGTTCGCATTCATCGTGCTGATTGGCGTGCTGCTGTTCCGCCCGTCCGGCATCATGGGCGAACGCGTTGCGGACCG
CGCATAAGAAGGGGAGCGCC

Downstream 100 bases:

>100_bases
TGGAGGCACGCACATGAGCAATAACAACGTCCTCCTCTCGATCCGAGGAGTCCAGAAGCGTTTCGGCGGCCTGCAAGCGC
TGTCCGACGTGAGCCTGGAA

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MTENFKAPMKTRAALYGFLILAIFAPFIVGAAGGNYWVRVLDFALLYIMLALGLNIVVGFAGLLDLGYIAFYAVGAYMMA
LLGSPHLSNQFEWIHNLFPNGLHLIVWWVIPLGAGLAALFGILLGTPVLKLRGDYLAIVTLGFGEIIRIFMNNLDRPVNI
TNGPKGINLIEPVKIFGFDFSKRHDFFGIRFDSVYMYYYLFVILAAIIIIVCLRLQNSRIGRAWVALREDEIAAKAMGIN
TRNIKLLAFAMGASFGGVSGAMFASFQGFVSPESFVLWESIYILAIVVLGGMGHIPGVILGGILLVGFQELLRALAEPVQ
NKLFGHVIVEAEVLRQLLFGLAMVVVMLYRPSGLWPSPRKEDRPQKQRAGGLSRI

Sequences:

>Translated_375_residues
MTENFKAPMKTRAALYGFLILAIFAPFIVGAAGGNYWVRVLDFALLYIMLALGLNIVVGFAGLLDLGYIAFYAVGAYMMA
LLGSPHLSNQFEWIHNLFPNGLHLIVWWVIPLGAGLAALFGILLGTPVLKLRGDYLAIVTLGFGEIIRIFMNNLDRPVNI
TNGPKGINLIEPVKIFGFDFSKRHDFFGIRFDSVYMYYYLFVILAAIIIIVCLRLQNSRIGRAWVALREDEIAAKAMGIN
TRNIKLLAFAMGASFGGVSGAMFASFQGFVSPESFVLWESIYILAIVVLGGMGHIPGVILGGILLVGFQELLRALAEPVQ
NKLFGHVIVEAEVLRQLLFGLAMVVVMLYRPSGLWPSPRKEDRPQKQRAGGLSRI
>Mature_374_residues
TENFKAPMKTRAALYGFLILAIFAPFIVGAAGGNYWVRVLDFALLYIMLALGLNIVVGFAGLLDLGYIAFYAVGAYMMAL
LGSPHLSNQFEWIHNLFPNGLHLIVWWVIPLGAGLAALFGILLGTPVLKLRGDYLAIVTLGFGEIIRIFMNNLDRPVNIT
NGPKGINLIEPVKIFGFDFSKRHDFFGIRFDSVYMYYYLFVILAAIIIIVCLRLQNSRIGRAWVALREDEIAAKAMGINT
RNIKLLAFAMGASFGGVSGAMFASFQGFVSPESFVLWESIYILAIVVLGGMGHIPGVILGGILLVGFQELLRALAEPVQN
KLFGHVIVEAEVLRQLLFGLAMVVVMLYRPSGLWPSPRKEDRPQKQRAGGLSRI

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=346, Percent_Identity=42.7745664739884, Blast_Score=223, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 41347; Mature: 41216

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENFKAPMKTRAALYGFLILAIFAPFIVGAAGGNYWVRVLDFALLYIMLALGLNIVVGF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLLDLGYIAFYAVGAYMMALLGSPHLSNQFEWIHNLFPNGLHLIVWWVIPLGAGLAALF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
GILLGTPVLKLRGDYLAIVTLGFGEIIRIFMNNLDRPVNITNGPKGINLIEPVKIFGFDF
HHHHCCCHHEECCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCC
SKRHDFFGIRFDSVYMYYYLFVILAAIIIIVCLRLQNSRIGRAWVALREDEIAAKAMGIN
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEECHHHHHHHHHCCC
TRNIKLLAFAMGASFGGVSGAMFASFQGFVSPESFVLWESIYILAIVVLGGMGHIPGVIL
CCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
GGILLVGFQELLRALAEPVQNKLFGHVIVEAEVLRQLLFGLAMVVVMLYRPSGLWPSPRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EDRPQKQRAGGLSRI
CCCCHHHHCCCCCCC
>Mature Secondary Structure 
TENFKAPMKTRAALYGFLILAIFAPFIVGAAGGNYWVRVLDFALLYIMLALGLNIVVGF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLLDLGYIAFYAVGAYMMALLGSPHLSNQFEWIHNLFPNGLHLIVWWVIPLGAGLAALF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
GILLGTPVLKLRGDYLAIVTLGFGEIIRIFMNNLDRPVNITNGPKGINLIEPVKIFGFDF
HHHHCCCHHEECCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCC
SKRHDFFGIRFDSVYMYYYLFVILAAIIIIVCLRLQNSRIGRAWVALREDEIAAKAMGIN
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEECHHHHHHHHHCCC
TRNIKLLAFAMGASFGGVSGAMFASFQGFVSPESFVLWESIYILAIVVLGGMGHIPGVIL
CCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
GGILLVGFQELLRALAEPVQNKLFGHVIVEAEVLRQLLFGLAMVVVMLYRPSGLWPSPRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EDRPQKQRAGGLSRI
CCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]