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The map label for this gene is cysD [H]

Identifier: 187929757

GI number: 187929757

Start: 2797980

End: 2798930

Strand: Reverse

Name: cysD [H]

Synonym: Rpic_2686

Alternate gene names: 187929757

Gene position: 2798930-2797980 (Counterclockwise)

Preceding gene: 187929758

Following gene: 187929756

Centisome position: 70.99

GC content: 65.09

Gene sequence:

>951_bases
ATGGGAGTCATGAGCGAAATCCCCGGCACGGCATTGACGCCTGTGCAGAACGAGCACCTCGACTGGCTGGAAGCCGAGTC
GATCTACATCATCCGCGAGGTGGTGGCCGAGTGCCGCAACCCGGCGCTGCTGTTCTCGGGCGGCAAGGATTCGATCGTCA
TGCTGCATCTGGCGCTCAAGGCCTTCCGCCTGGGCGAGCGCAAGATCGAGCTGCCGTTCCCGCTGGTGCACATCGACACG
GGTCACAACTACCCCGAGGTGATCACCTTCCGCGACGAGCAGGTCGCCAAGCTGGGTGCTCGCCTGGTGGTGGGCCATGT
GGAGGATTCCATCAAGCGCGGCACCGTGCGCCTGCGTAAAGAGACCGATTCGCGCAACGCCGCACAGGCCGTCACGCTGC
TGGAGACGATCGAATCGCACGGCTTCGACGCCCTGATGGGCGGTGCCCGCCGCGACGAAGAAAAGGCCCGTGCGAAGGAG
CGCATCTTCTCGTTCCGCGACGAGTTCGGCCAGTGGGATCCGAAGGCCCAGCGCCCGGAACTCTGGAGCTTGTACAACGC
ACGCATGGCGCAGGGCGAGCAGATGCGCGTGTTCCCGATCTCCAACTGGACGGAACTCGACGTGTGGCAGTACATCGCCC
GCGAGAACCTCGCCCTGCCGCCGATCTACTACGCGCACGAGCGTGAAGTGGTGCGCAAGAACGGCCTGCTGGTGCCCGTC
ACGTCGATCACGCCCAAGGCCGACGGCGACGTGAGCGAAGTCCTGTCGGTGCGCTTCCGCACCGTGGGCGACATCAGCTG
CACATGCCCGGTGGCGAGCACCGCCGCCACGCCGGCCGAGATCATCGCCGAGACGGCCGTGACCGAAATCACCGAACGCG
GCGCCACGCGCATGGACGACCAGACGAGCGAAGCCTCGATGGAAAAGCGCAAGAAGGAAGGGTATTTCTGA

Upstream 100 bases:

>100_bases
GAGGATGTGCGCGCCGGCCGCTGGTGGTGGGAATCGCGCGACAGCAAGGAATGCGGTCTGCATGCGACGAATCTGTCGCA
AAAGAGCTAAGGAAAGGAAC

Downstream 100 bases:

>100_bases
TCATGACGACGAATTCGACGCACCCAGCGACGCATCCTGCATCGCACCAAGGCCTGCTGCGCTTCATCACCGCCGGTTCC
GTTGACGACGGCAAGAGCAC

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MGVMSEIPGTALTPVQNEHLDWLEAESIYIIREVVAECRNPALLFSGGKDSIVMLHLALKAFRLGERKIELPFPLVHIDT
GHNYPEVITFRDEQVAKLGARLVVGHVEDSIKRGTVRLRKETDSRNAAQAVTLLETIESHGFDALMGGARRDEEKARAKE
RIFSFRDEFGQWDPKAQRPELWSLYNARMAQGEQMRVFPISNWTELDVWQYIARENLALPPIYYAHEREVVRKNGLLVPV
TSITPKADGDVSEVLSVRFRTVGDISCTCPVASTAATPAEIIAETAVTEITERGATRMDDQTSEASMEKRKKEGYF

Sequences:

>Translated_316_residues
MGVMSEIPGTALTPVQNEHLDWLEAESIYIIREVVAECRNPALLFSGGKDSIVMLHLALKAFRLGERKIELPFPLVHIDT
GHNYPEVITFRDEQVAKLGARLVVGHVEDSIKRGTVRLRKETDSRNAAQAVTLLETIESHGFDALMGGARRDEEKARAKE
RIFSFRDEFGQWDPKAQRPELWSLYNARMAQGEQMRVFPISNWTELDVWQYIARENLALPPIYYAHEREVVRKNGLLVPV
TSITPKADGDVSEVLSVRFRTVGDISCTCPVASTAATPAEIIAETAVTEITERGATRMDDQTSEASMEKRKKEGYF
>Mature_315_residues
GVMSEIPGTALTPVQNEHLDWLEAESIYIIREVVAECRNPALLFSGGKDSIVMLHLALKAFRLGERKIELPFPLVHIDTG
HNYPEVITFRDEQVAKLGARLVVGHVEDSIKRGTVRLRKETDSRNAAQAVTLLETIESHGFDALMGGARRDEEKARAKER
IFSFRDEFGQWDPKAQRPELWSLYNARMAQGEQMRVFPISNWTELDVWQYIARENLALPPIYYAHEREVVRKNGLLVPVT
SITPKADGDVSEVLSVRFRTVGDISCTCPVASTAATPAEIIAETAVTEITERGATRMDDQTSEASMEKRKKEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=303, Percent_Identity=47.1947194719472, Blast_Score=261, Evalue=6e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 35557; Mature: 35426

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVMSEIPGTALTPVQNEHLDWLEAESIYIIREVVAECRNPALLFSGGKDSIVMLHLALK
CCCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHH
AFRLGERKIELPFPLVHIDTGHNYPEVITFRDEQVAKLGARLVVGHVEDSIKRGTVRLRK
HHHCCCEEEECCCCEEEEECCCCCCCEEEECHHHHHHHHHHEEEEHHHHHHHHCCEEEEE
ETDSRNAAQAVTLLETIESHGFDALMGGARRDEEKARAKERIFSFRDEFGQWDPKAQRPE
CCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCH
LWSLYNARMAQGEQMRVFPISNWTELDVWQYIARENLALPPIYYAHEREVVRKNGLLVPV
HHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCCEEEE
TSITPKADGDVSEVLSVRFRTVGDISCTCPVASTAATPAEIIAETAVTEITERGATRMDD
CCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
QTSEASMEKRKKEGYF
CHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GVMSEIPGTALTPVQNEHLDWLEAESIYIIREVVAECRNPALLFSGGKDSIVMLHLALK
CCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHH
AFRLGERKIELPFPLVHIDTGHNYPEVITFRDEQVAKLGARLVVGHVEDSIKRGTVRLRK
HHHCCCEEEECCCCEEEEECCCCCCCEEEECHHHHHHHHHHEEEEHHHHHHHHCCEEEEE
ETDSRNAAQAVTLLETIESHGFDALMGGARRDEEKARAKERIFSFRDEFGQWDPKAQRPE
CCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCH
LWSLYNARMAQGEQMRVFPISNWTELDVWQYIARENLALPPIYYAHEREVVRKNGLLVPV
HHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCCEEEE
TSITPKADGDVSEVLSVRFRTVGDISCTCPVASTAATPAEIIAETAVTEITERGATRMDD
CCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
QTSEASMEKRKKEGYF
CHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]