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The map label for this gene is alkK [H]

Identifier: 187929701

GI number: 187929701

Start: 2743483

End: 2745159

Strand: Reverse

Name: alkK [H]

Synonym: Rpic_2630

Alternate gene names: 187929701

Gene position: 2745159-2743483 (Counterclockwise)

Preceding gene: 187929702

Following gene: 187929700

Centisome position: 69.63

GC content: 63.92

Gene sequence:

>1677_bases
ATGACCGACACCATCCAGCCCACCAAGCCTGTCCGCGGCTGCCCGTCCACCACGGGCAACGAGCGCCAACTGAACACCAC
AACGCTGATCCGGCACGCTGCGCGCACCCACGGGGACCAGGAGATCGTCTACCGCACACCCGATGGCGGCTGGGATCGCT
ACACCTATGCCGATTGCTACGCGCGCGTCTGCCGCAGCGCCAATGCACTGCGCGCTCTCGGCGTCGAGCCGGGCGACCGG
GTCGGCATCCTGGACTGGAACAGCCGACGCCACTTCGAGCTGTACTGGTCCATTCCCGGCCTGGGTGCTGTCATGTTGCA
GATGAATCTGCGCCTGGGCCCAGAGGACCTGGGCTACGTGGTCGACCACAGCAAGGTGTCCTACGTTTGCGTAGACGAGT
CATTGCTACCCCTTGCCGAATCCGTCGCAGCGAATTCGCCCCAGATCAAGGGCTGGATTGTCATGACCGACAAGCCGCTG
GACCAGATCAAGACCACGCTGAAACCGCTGCTGCACTACGAAGACCTGCTGGCCGCCGCCGACACGAAGATCGACTGGCC
CGAGATCGACGAAACCTCGGCCTACAGCGCCTGCTACACCACCGGCACCACGGGCAAGCCCAAGGGCGTGTACTACTCGC
ACCGCGGCATCTACCTGCACTCCACGGCCATGGCCACCAATCTGGGCATGACGCTGGACGACTGCGTCATGCTCATCACG
CCCATGTTCCACGGGCAATGCTGGGGCCTGCCGCAGGCCGCCACGCTGCTCGCCGACAAGATCGTGCTGCCGGGCCGCTA
CGTTGCCGAAGACACCAAGCCGCTGGTCGATGCCATGATCGCCGAGGGCGTGACCATCGCCAATGGCGCACCGGCCATCT
TCCAGCCCATGCTGCAGTACATCGAGACGATGCCGGTCAAGCCGGACTTCAGCCGCATGCGCATGCTGTCTGGCGCCACG
GAGCCGCCGCTGTCGATGATGATCGGTTTCTACGACCTCACGGGTGCTGAGGTGGTGCACGCCTACGGCGCCACCGAAGC
CACGACGCTGGTGACGATGAACCGCCTCAAGTCCACGCTCAAGAAGCGCTTGACCGAGGAAGAGAAGTGGAACCTCAAGC
GCAAGCAGGGTCTGGTGCTGACCGGGGTGGATATTCGCATCCTCGATGCCGACGACAAGGACTTGCCGCACGACGGAAAG
TCAGCGGGCGAGATTTGCCTGCGCGGCCCCTGGATAACGGCCAGATACCACGACATGCCTGATTCCGCAGACCGTTTCCT
GGAAGGCGGATGGTGGCGCTCGGGCGATGTCGGCACGGTGGACGAGAACGGCTACCTCAAAGTCACCGACCGCATCAAGG
ACGTGATCAAGAGCGGTGGTGAATGGATTTCTTCCATCGACATGGAAAACCTGCTCATGGGCCACCCGGCCGTGCGCGAC
GCCGCGGTGGTCGGCATTCCCCATGCGAAGTGGCAGGAACGGCCGCTGGCCCTGGTGGTGCTGAGGCCCGGCCAGCAGGC
GACTCAGGAGCAATTGCAGGAACACCTGACCAGCGCCTTCGCCAAGTGGCAGTTGCCAGATCAGGTCCTGTTCGTCGAAG
CCATCCCCAAGACCAGCGTCGGCAAGCTCGACAAGAAGCGCATCCGCGCCGAGCATGCGGGCCGCTACGCCGAGTGA

Upstream 100 bases:

>100_bases
CAGCACGGGGCATTTACCAGATCACCCCATCCGGCGCCATCGAAGCGGCCCCGGCCCCACGGTTCCAGGCACTCGACACA
ACTACCTAGGAGACAAGCAC

Downstream 100 bases:

>100_bases
GCCAACCTTTTTGCACCAGGAGAAGAACATGAATGACCATGAACCCGTCATCGTCGGTGCGTTGCGCACCCCCGTGGGCA
AGCGCGGCGGGCGCCTGCAG

Product: AMP-dependent synthetase and ligase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 558; Mature: 557

Protein sequence:

>558_residues
MTDTIQPTKPVRGCPSTTGNERQLNTTTLIRHAARTHGDQEIVYRTPDGGWDRYTYADCYARVCRSANALRALGVEPGDR
VGILDWNSRRHFELYWSIPGLGAVMLQMNLRLGPEDLGYVVDHSKVSYVCVDESLLPLAESVAANSPQIKGWIVMTDKPL
DQIKTTLKPLLHYEDLLAAADTKIDWPEIDETSAYSACYTTGTTGKPKGVYYSHRGIYLHSTAMATNLGMTLDDCVMLIT
PMFHGQCWGLPQAATLLADKIVLPGRYVAEDTKPLVDAMIAEGVTIANGAPAIFQPMLQYIETMPVKPDFSRMRMLSGAT
EPPLSMMIGFYDLTGAEVVHAYGATEATTLVTMNRLKSTLKKRLTEEEKWNLKRKQGLVLTGVDIRILDADDKDLPHDGK
SAGEICLRGPWITARYHDMPDSADRFLEGGWWRSGDVGTVDENGYLKVTDRIKDVIKSGGEWISSIDMENLLMGHPAVRD
AAVVGIPHAKWQERPLALVVLRPGQQATQEQLQEHLTSAFAKWQLPDQVLFVEAIPKTSVGKLDKKRIRAEHAGRYAE

Sequences:

>Translated_558_residues
MTDTIQPTKPVRGCPSTTGNERQLNTTTLIRHAARTHGDQEIVYRTPDGGWDRYTYADCYARVCRSANALRALGVEPGDR
VGILDWNSRRHFELYWSIPGLGAVMLQMNLRLGPEDLGYVVDHSKVSYVCVDESLLPLAESVAANSPQIKGWIVMTDKPL
DQIKTTLKPLLHYEDLLAAADTKIDWPEIDETSAYSACYTTGTTGKPKGVYYSHRGIYLHSTAMATNLGMTLDDCVMLIT
PMFHGQCWGLPQAATLLADKIVLPGRYVAEDTKPLVDAMIAEGVTIANGAPAIFQPMLQYIETMPVKPDFSRMRMLSGAT
EPPLSMMIGFYDLTGAEVVHAYGATEATTLVTMNRLKSTLKKRLTEEEKWNLKRKQGLVLTGVDIRILDADDKDLPHDGK
SAGEICLRGPWITARYHDMPDSADRFLEGGWWRSGDVGTVDENGYLKVTDRIKDVIKSGGEWISSIDMENLLMGHPAVRD
AAVVGIPHAKWQERPLALVVLRPGQQATQEQLQEHLTSAFAKWQLPDQVLFVEAIPKTSVGKLDKKRIRAEHAGRYAE
>Mature_557_residues
TDTIQPTKPVRGCPSTTGNERQLNTTTLIRHAARTHGDQEIVYRTPDGGWDRYTYADCYARVCRSANALRALGVEPGDRV
GILDWNSRRHFELYWSIPGLGAVMLQMNLRLGPEDLGYVVDHSKVSYVCVDESLLPLAESVAANSPQIKGWIVMTDKPLD
QIKTTLKPLLHYEDLLAAADTKIDWPEIDETSAYSACYTTGTTGKPKGVYYSHRGIYLHSTAMATNLGMTLDDCVMLITP
MFHGQCWGLPQAATLLADKIVLPGRYVAEDTKPLVDAMIAEGVTIANGAPAIFQPMLQYIETMPVKPDFSRMRMLSGATE
PPLSMMIGFYDLTGAEVVHAYGATEATTLVTMNRLKSTLKKRLTEEEKWNLKRKQGLVLTGVDIRILDADDKDLPHDGKS
AGEICLRGPWITARYHDMPDSADRFLEGGWWRSGDVGTVDENGYLKVTDRIKDVIKSGGEWISSIDMENLLMGHPAVRDA
AVVGIPHAKWQERPLALVVLRPGQQATQEQLQEHLTSAFAKWQLPDQVLFVEAIPKTSVGKLDKKRIRAEHAGRYAE

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=541, Percent_Identity=25.8780036968577, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI187761343, Length=541, Percent_Identity=25.8780036968577, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI156151445, Length=536, Percent_Identity=23.134328358209, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI38505220, Length=519, Percent_Identity=24.0847784200385, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI58082049, Length=504, Percent_Identity=25.9920634920635, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI157311624, Length=504, Percent_Identity=25.7936507936508, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI157311622, Length=504, Percent_Identity=25.7936507936508, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI42544132, Length=468, Percent_Identity=23.9316239316239, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI28416953, Length=574, Percent_Identity=21.7770034843206, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI115511026, Length=480, Percent_Identity=24.1666666666667, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI122937307, Length=510, Percent_Identity=23.921568627451, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI13375727, Length=566, Percent_Identity=22.6148409893993, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI221142682, Length=527, Percent_Identity=24.4781783681214, Blast_Score=151, Evalue=9e-38,
Organism=Escherichia coli, GI145693145, Length=360, Percent_Identity=30.5555555555556, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1788107, Length=511, Percent_Identity=23.8747553816047, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1786810, Length=361, Percent_Identity=27.1468144044321, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1790505, Length=607, Percent_Identity=22.5700164744646, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789201, Length=365, Percent_Identity=24.1095890410959, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=570, Percent_Identity=26.8421052631579, Blast_Score=159, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17538037, Length=559, Percent_Identity=24.865831842576, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17557194, Length=553, Percent_Identity=24.7739602169982, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17559526, Length=453, Percent_Identity=24.9448123620309, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI32563687, Length=524, Percent_Identity=23.8549618320611, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17560308, Length=538, Percent_Identity=25.6505576208178, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71994694, Length=555, Percent_Identity=23.0630630630631, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71994703, Length=555, Percent_Identity=23.0630630630631, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71994690, Length=558, Percent_Identity=23.1182795698925, Blast_Score=112, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17531443, Length=525, Percent_Identity=22.4761904761905, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17560140, Length=391, Percent_Identity=26.5984654731458, Blast_Score=105, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=361, Percent_Identity=22.9916897506925, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71996755, Length=519, Percent_Identity=22.3506743737958, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71983001, Length=407, Percent_Identity=23.0958230958231, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71982997, Length=407, Percent_Identity=23.0958230958231, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=361, Percent_Identity=30.1939058171745, Blast_Score=137, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6319264, Length=617, Percent_Identity=22.0421393841167, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21355181, Length=567, Percent_Identity=25.3968253968254, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24581924, Length=546, Percent_Identity=23.8095238095238, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI18859661, Length=408, Percent_Identity=27.9411764705882, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI19922652, Length=534, Percent_Identity=24.3445692883895, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648260, Length=182, Percent_Identity=27.4725274725275, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281365686, Length=381, Percent_Identity=24.6719160104987, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24653035, Length=501, Percent_Identity=23.5528942115768, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648253, Length=515, Percent_Identity=22.7184466019417, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21356947, Length=176, Percent_Identity=28.9772727272727, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24648257, Length=218, Percent_Identity=26.605504587156, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI21356441, Length=506, Percent_Identity=21.5415019762846, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI20130357, Length=214, Percent_Identity=26.1682242990654, Blast_Score=66, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 61979; Mature: 61847

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTIQPTKPVRGCPSTTGNERQLNTTTLIRHAARTHGDQEIVYRTPDGGWDRYTYADCY
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHH
ARVCRSANALRALGVEPGDRVGILDWNSRRHFELYWSIPGLGAVMLQMNLRLGPEDLGYV
HHHHCCCCHHHCCCCCCCCEEEEEECCCCCEEEEEEECCCCCEEEEEEECCCCHHHHCEE
VDHSKVSYVCVDESLLPLAESVAANSPQIKGWIVMTDKPLDQIKTTLKPLLHYEDLLAAA
EECCCEEEEEECCCHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHC
DTKIDWPEIDETSAYSACYTTGTTGKPKGVYYSHRGIYLHSTAMATNLGMTLDDCVMLIT
CCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEECCCEEEEEHHHHHHCCCCHHHHHHHHH
PMFHGQCWGLPQAATLLADKIVLPGRYVAEDTKPLVDAMIAEGVTIANGAPAIFQPMLQY
HHHCCCCCCCCHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHCCEEEECCCCHHHHHHHHH
IETMPVKPDFSRMRMLSGATEPPLSMMIGFYDLTGAEVVHAYGATEATTLVTMNRLKSTL
HHHCCCCCCHHHHHHHCCCCCCCHHHHHEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHH
KKRLTEEEKWNLKRKQGLVLTGVDIRILDADDKDLPHDGKSAGEICLRGPWITARYHDMP
HHHCCCHHHCCCHHHCCEEEECCEEEEECCCCCCCCCCCCCCCCEEEECCEEEEEECCCC
DSADRFLEGGWWRSGDVGTVDENGYLKVTDRIKDVIKSGGEWISSIDMENLLMGHPAVRD
CHHHHHHCCCCCCCCCCCEECCCCEEEEHHHHHHHHHCCCHHHHHCCHHHHHHCCCCCCC
AAVVGIPHAKWQERPLALVVLRPGQQATQEQLQEHLTSAFAKWQLPDQVLFVEAIPKTSV
EEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
GKLDKKRIRAEHAGRYAE
CCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TDTIQPTKPVRGCPSTTGNERQLNTTTLIRHAARTHGDQEIVYRTPDGGWDRYTYADCY
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHH
ARVCRSANALRALGVEPGDRVGILDWNSRRHFELYWSIPGLGAVMLQMNLRLGPEDLGYV
HHHHCCCCHHHCCCCCCCCEEEEEECCCCCEEEEEEECCCCCEEEEEEECCCCHHHHCEE
VDHSKVSYVCVDESLLPLAESVAANSPQIKGWIVMTDKPLDQIKTTLKPLLHYEDLLAAA
EECCCEEEEEECCCHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHC
DTKIDWPEIDETSAYSACYTTGTTGKPKGVYYSHRGIYLHSTAMATNLGMTLDDCVMLIT
CCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEECCCEEEEEHHHHHHCCCCHHHHHHHHH
PMFHGQCWGLPQAATLLADKIVLPGRYVAEDTKPLVDAMIAEGVTIANGAPAIFQPMLQY
HHHCCCCCCCCHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHCCEEEECCCCHHHHHHHHH
IETMPVKPDFSRMRMLSGATEPPLSMMIGFYDLTGAEVVHAYGATEATTLVTMNRLKSTL
HHHCCCCCCHHHHHHHCCCCCCCHHHHHEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHH
KKRLTEEEKWNLKRKQGLVLTGVDIRILDADDKDLPHDGKSAGEICLRGPWITARYHDMP
HHHCCCHHHCCCHHHCCEEEECCEEEEECCCCCCCCCCCCCCCCEEEECCEEEEEECCCC
DSADRFLEGGWWRSGDVGTVDENGYLKVTDRIKDVIKSGGEWISSIDMENLLMGHPAVRD
CHHHHHHCCCCCCCCCCCEECCCCEEEEHHHHHHHHHCCCHHHHHCCHHHHHHCCCCCCC
AAVVGIPHAKWQERPLALVVLRPGQQATQEQLQEHLTSAFAKWQLPDQVLFVEAIPKTSV
EEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
GKLDKKRIRAEHAGRYAE
CCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]