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The map label for this gene is braE [H]

Identifier: 187929531

GI number: 187929531

Start: 2562699

End: 2563733

Strand: Reverse

Name: braE [H]

Synonym: Rpic_2452

Alternate gene names: 187929531

Gene position: 2563733-2562699 (Counterclockwise)

Preceding gene: 187929532

Following gene: 187929530

Centisome position: 65.03

GC content: 63.57

Gene sequence:

>1035_bases
ATGAGCGGGCTGCAGCAATTGAGGCGGTCTGCACGGCCTGTTGAGGGCTCTGCGGCGCATGGGTTGCCGCAAGCGCGTTG
GTCGCCGTTTGAGATCGCGTTCTGGTGCGTGCCGATCGCGGTGTTCTTTCTTCTTCCCGATTACCTCGTCTTCGGCAGCC
AGGTGTTGATCGTCGGGCTGTTTGCGTTGTCGCTCGACCTGGTGCTCGGCTATGCGGGCATCGTGTCGCTCGGACATGCA
GGCTTCTTCGGGCTTGGCGCCTATACCGCCGGACTGCTCGCGGCACATGGCTGGGGTGAACCGCTCACCGGTCTGGCCGC
GGGCGCTGTCGTCGCTGCGCTGGCCGGCTTCTGTGTGAGCTTTCTCGTCGTGCGCGGGCAGGATCTGACACGACTGATGG
TCACGCTCGGCATCGGTCTGATGCTGTATGAGGCGGCCAACAAGATGGCGTTTCTCACGGGCGGTGTGGACGGCCTTTCC
GGTGTGGCGATGCGCAACCTGCTCGGCACATTTGAGTTCGATCTGGAGGGGCGCACGGCTTACTTCTATAGCTTGGTCGT
GCTGTTTCTGGTGTTTGTGGGGTTGCGTCGCCTGGTGCGCTCGCCGTTTGGGTTGTCGCTGCGCGGCATTCAACAGGGGC
CGAAGCGCATGCCGGCTATCGGCGCGAATGTTCGCATGCGGCTGGTGGCGGCGTTCACCGTCAGCGCAGCGATTGCCGGT
GTCGCAGGCGGGCTGCTCGCGCAGACCACGCAATTTGTCGGTCTGGATTCGCTGGGCTTCTCGCGCTCGGCAGAGTTGCT
GATCATGCTCGTGCTGGGCGGTGCAGGCCGGTTGTACGGCGCGTTGATTGGCGCAGCGGTGTTCATGATTGCGCAGGACG
TGTTGGCCGGCATCAATCCCGTCTACTGGCAGTTCTGGATCGGCCTGCTGCTGATGGTGATCGTGTTGTTTGCGCGGGGT
GGCATTCTCGGTGGGCTGGAAACTGCGGTGTCTGCGGTCAGGGCGCGGCGCGCTCGCAAGGGAGGTGCTTCATGA

Upstream 100 bases:

>100_bases
CGGGCAAGTACTACGTGCCGGACGTGGGCGCGTTCGTCATCTATGGGTTGATGGTCGTGCTGCTCGTGCTCTTCCCGGGC
GGTTTGCTGGGGAGGCGCGC

Downstream 100 bases:

>100_bases
CTGCGCCCACGATCCATCCTCCGCCCACATTGAGCACGCGTGGCCTGTCCAGGCGCTGGGGCGGCTTTCACGCCAATTCC
GATATCTCGCTGTCTTTTGC

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSGLQQLRRSARPVEGSAAHGLPQARWSPFEIAFWCVPIAVFFLLPDYLVFGSQVLIVGLFALSLDLVLGYAGIVSLGHA
GFFGLGAYTAGLLAAHGWGEPLTGLAAGAVVAALAGFCVSFLVVRGQDLTRLMVTLGIGLMLYEAANKMAFLTGGVDGLS
GVAMRNLLGTFEFDLEGRTAYFYSLVVLFLVFVGLRRLVRSPFGLSLRGIQQGPKRMPAIGANVRMRLVAAFTVSAAIAG
VAGGLLAQTTQFVGLDSLGFSRSAELLIMLVLGGAGRLYGALIGAAVFMIAQDVLAGINPVYWQFWIGLLLMVIVLFARG
GILGGLETAVSAVRARRARKGGAS

Sequences:

>Translated_344_residues
MSGLQQLRRSARPVEGSAAHGLPQARWSPFEIAFWCVPIAVFFLLPDYLVFGSQVLIVGLFALSLDLVLGYAGIVSLGHA
GFFGLGAYTAGLLAAHGWGEPLTGLAAGAVVAALAGFCVSFLVVRGQDLTRLMVTLGIGLMLYEAANKMAFLTGGVDGLS
GVAMRNLLGTFEFDLEGRTAYFYSLVVLFLVFVGLRRLVRSPFGLSLRGIQQGPKRMPAIGANVRMRLVAAFTVSAAIAG
VAGGLLAQTTQFVGLDSLGFSRSAELLIMLVLGGAGRLYGALIGAAVFMIAQDVLAGINPVYWQFWIGLLLMVIVLFARG
GILGGLETAVSAVRARRARKGGAS
>Mature_343_residues
SGLQQLRRSARPVEGSAAHGLPQARWSPFEIAFWCVPIAVFFLLPDYLVFGSQVLIVGLFALSLDLVLGYAGIVSLGHAG
FFGLGAYTAGLLAAHGWGEPLTGLAAGAVVAALAGFCVSFLVVRGQDLTRLMVTLGIGLMLYEAANKMAFLTGGVDGLSG
VAMRNLLGTFEFDLEGRTAYFYSLVVLFLVFVGLRRLVRSPFGLSLRGIQQGPKRMPAIGANVRMRLVAAFTVSAAIAGV
AGGLLAQTTQFVGLDSLGFSRSAELLIMLVLGGAGRLYGALIGAAVFMIAQDVLAGINPVYWQFWIGLLLMVIVLFARGG
ILGGLETAVSAVRARRARKGGAS

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=292, Percent_Identity=29.7945205479452, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 36102; Mature: 35971

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLQQLRRSARPVEGSAAHGLPQARWSPFEIAFWCVPIAVFFLLPDYLVFGSQVLIVGL
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
FALSLDLVLGYAGIVSLGHAGFFGLGAYTAGLLAAHGWGEPLTGLAAGAVVAALAGFCVS
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
FLVVRGQDLTRLMVTLGIGLMLYEAANKMAFLTGGVDGLSGVAMRNLLGTFEFDLEGRTA
HHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHEECCCCCHH
YFYSLVVLFLVFVGLRRLVRSPFGLSLRGIQQGPKRMPAIGANVRMRLVAAFTVSAAIAG
HHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHH
VAGGLLAQTTQFVGLDSLGFSRSAELLIMLVLGGAGRLYGALIGAAVFMIAQDVLAGINP
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCH
VYWQFWIGLLLMVIVLFARGGILGGLETAVSAVRARRARKGGAS
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SGLQQLRRSARPVEGSAAHGLPQARWSPFEIAFWCVPIAVFFLLPDYLVFGSQVLIVGL
CHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
FALSLDLVLGYAGIVSLGHAGFFGLGAYTAGLLAAHGWGEPLTGLAAGAVVAALAGFCVS
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
FLVVRGQDLTRLMVTLGIGLMLYEAANKMAFLTGGVDGLSGVAMRNLLGTFEFDLEGRTA
HHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHEECCCCCHH
YFYSLVVLFLVFVGLRRLVRSPFGLSLRGIQQGPKRMPAIGANVRMRLVAAFTVSAAIAG
HHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHH
VAGGLLAQTTQFVGLDSLGFSRSAELLIMLVLGGAGRLYGALIGAAVFMIAQDVLAGINP
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCH
VYWQFWIGLLLMVIVLFARGGILGGLETAVSAVRARRARKGGAS
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]