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The map label for this gene is dnaB [H]

Identifier: 187929404

GI number: 187929404

Start: 2434169

End: 2435563

Strand: Reverse

Name: dnaB [H]

Synonym: Rpic_2325

Alternate gene names: 187929404

Gene position: 2435563-2434169 (Counterclockwise)

Preceding gene: 187929405

Following gene: 187929403

Centisome position: 61.78

GC content: 62.29

Gene sequence:

>1395_bases
ATGACTGGCCCGGACGATCTTCCGCAAGCGCGCCCACTGTACGCGATCGAGGCCGAACAGGCGGTTCTTGGCGGCCTCCT
GCTCGACAACAACGCGATCGATCGCGTTGGCGGTCTTGAGCCGGCGCACATCTATCGCCATGACCATCGCGAGATTCTCC
GCGCGATCGTGCGTCTCATCATTGCTGGCAAGCCTGCCGATGTCGTGACCGTGTTCGAATCGCTGCAGGCTGCAGGAAAG
GCCGTGGATGTTGGTGGCCTGTCCTATCTGAACTCGCTGGCACAGAACACGCCGAGCGCTGCCAACATCGCGCGCTACGC
CGAAATCGTGAAAGATCGCGCGCTACTACGCGAGACCCTGGCCACGGCCCGCAAGGTGCAGGAACTGGTAGAGACGCCGA
GCGCGATGAAGGGCACCGAGATCCTGGATCGTGCGCAGACGATGCTGGCCAATCTCGCCCAGGTTGGCGTGCGCCGCGAG
CCGAAGATGCTGCGCGAACTGCTTGCGCCGTTCGTTGAAGAAGTCGACAACCGCGCAACTGGCGCGATCGAATCAGCCAT
CCCAACGGGCCTGGATGCTCTTGATCAGGCGCTCAACGGCGGAATGCGTCGCGGGAACCTGATCATCGTCGCCGGCAGGC
CGTCGATGGGCAAGACAGCCCTGACCACCGATATTGGCCTGAACGTTGCGACCGACTACAACGTCCTGCTGCTGTCGATG
GAAATGTCCGACCAGGAGATCGTGGCACGTGCCCTGGCCAGCCGCGGTGGCATTCCGCTCTCGAAGCTACTGGGCCGCAT
GAACCACGGTGATGACCACGCATGGACGAACCTGACTGCTGGCGTTGTCGCGATCGACCAACTCAACTTCGCCGTCGATG
ACTCGCCGGCGCTGACGCTTCTGGACGTGCGCATGAAAGCCAAGGGCCATCAGCGCAAGTACGGGCTCGACCTGCTGATC
GTCGACTACGTGGGCCTCATGACTGGTGGCGAGGAAAAGATGCGTACGCAGCAGATTGGCGCTTACTCCCGAGGCCTCAA
GGCGCTGGCCAAGGAACTCAATGTGCCAGTGATGGCGCTGGCGCAACTCAATCGCAAGAACGAAGACCGACCGGATAAGA
AACCAATCCTCGCAGACCTGCGGGACTCGGGCGACATCGAGCAGGACGCCGACGTGGTGATGTTCGTGCATCGCCCCGAG
ATGTACGACCACAACAACGCGGAGCTCAAGGGCTATGCCGAAGTGCTGATCCGCAAGCAGCGCAACGGCGCGCTCGGCGA
TGTAGCGCTCGAATACAAGGGCGCGCTGACGAAGTTCTGCGAGTGGACCGGCCCGCTGCCTGTCATCGGTACCGGATCGC
CGGTGCGCAAGCGCGGCATTGCCGAATACCTGTGA

Upstream 100 bases:

>100_bases
GACGTAGCAAACCGTTCCGTGCAAGCCTGGATTGGATCGTCAAATCGGAGAACTTCGCGAAGATCCGTGAAGGTCGGTAT
CACCCTGACCAGGAGGCGGC

Downstream 100 bases:

>100_bases
GGACGACATGCCAAAAATCACGCTAAGCCTCCCGTACCCGATCAGCGCGAACCGCTACTGGGCTTCTCGCATCGTCAAGC
CTAAGAGCGGTCCGAGCTTC

Product: DnaB domain-containing protein helicase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MTGPDDLPQARPLYAIEAEQAVLGGLLLDNNAIDRVGGLEPAHIYRHDHREILRAIVRLIIAGKPADVVTVFESLQAAGK
AVDVGGLSYLNSLAQNTPSAANIARYAEIVKDRALLRETLATARKVQELVETPSAMKGTEILDRAQTMLANLAQVGVRRE
PKMLRELLAPFVEEVDNRATGAIESAIPTGLDALDQALNGGMRRGNLIIVAGRPSMGKTALTTDIGLNVATDYNVLLLSM
EMSDQEIVARALASRGGIPLSKLLGRMNHGDDHAWTNLTAGVVAIDQLNFAVDDSPALTLLDVRMKAKGHQRKYGLDLLI
VDYVGLMTGGEEKMRTQQIGAYSRGLKALAKELNVPVMALAQLNRKNEDRPDKKPILADLRDSGDIEQDADVVMFVHRPE
MYDHNNAELKGYAEVLIRKQRNGALGDVALEYKGALTKFCEWTGPLPVIGTGSPVRKRGIAEYL

Sequences:

>Translated_464_residues
MTGPDDLPQARPLYAIEAEQAVLGGLLLDNNAIDRVGGLEPAHIYRHDHREILRAIVRLIIAGKPADVVTVFESLQAAGK
AVDVGGLSYLNSLAQNTPSAANIARYAEIVKDRALLRETLATARKVQELVETPSAMKGTEILDRAQTMLANLAQVGVRRE
PKMLRELLAPFVEEVDNRATGAIESAIPTGLDALDQALNGGMRRGNLIIVAGRPSMGKTALTTDIGLNVATDYNVLLLSM
EMSDQEIVARALASRGGIPLSKLLGRMNHGDDHAWTNLTAGVVAIDQLNFAVDDSPALTLLDVRMKAKGHQRKYGLDLLI
VDYVGLMTGGEEKMRTQQIGAYSRGLKALAKELNVPVMALAQLNRKNEDRPDKKPILADLRDSGDIEQDADVVMFVHRPE
MYDHNNAELKGYAEVLIRKQRNGALGDVALEYKGALTKFCEWTGPLPVIGTGSPVRKRGIAEYL
>Mature_463_residues
TGPDDLPQARPLYAIEAEQAVLGGLLLDNNAIDRVGGLEPAHIYRHDHREILRAIVRLIIAGKPADVVTVFESLQAAGKA
VDVGGLSYLNSLAQNTPSAANIARYAEIVKDRALLRETLATARKVQELVETPSAMKGTEILDRAQTMLANLAQVGVRREP
KMLRELLAPFVEEVDNRATGAIESAIPTGLDALDQALNGGMRRGNLIIVAGRPSMGKTALTTDIGLNVATDYNVLLLSME
MSDQEIVARALASRGGIPLSKLLGRMNHGDDHAWTNLTAGVVAIDQLNFAVDDSPALTLLDVRMKAKGHQRKYGLDLLIV
DYVGLMTGGEEKMRTQQIGAYSRGLKALAKELNVPVMALAQLNRKNEDRPDKKPILADLRDSGDIEQDADVVMFVHRPEM
YDHNNAELKGYAEVLIRKQRNGALGDVALEYKGALTKFCEWTGPLPVIGTGSPVRKRGIAEYL

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=444, Percent_Identity=40.3153153153153, Blast_Score=304, Evalue=6e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50398; Mature: 50267

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGPDDLPQARPLYAIEAEQAVLGGLLLDNNAIDRVGGLEPAHIYRHDHREILRAIVRLI
CCCCCCCCCCCCEEEEEHHHHHHHHEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IAGKPADVVTVFESLQAAGKAVDVGGLSYLNSLAQNTPSAANIARYAEIVKDRALLRETL
HCCCCHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ATARKVQELVETPSAMKGTEILDRAQTMLANLAQVGVRREPKMLRELLAPFVEEVDNRAT
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHH
GAIESAIPTGLDALDQALNGGMRRGNLIIVAGRPSMGKTALTTDIGLNVATDYNVLLLSM
HHHHHHCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCEEECCCEEEEEEE
EMSDQEIVARALASRGGIPLSKLLGRMNHGDDHAWTNLTAGVVAIDQLNFAVDDSPALTL
CCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHEEEECCCEEECCCCCEEE
LDVRMKAKGHQRKYGLDLLIVDYVGLMTGGEEKMRTQQIGAYSRGLKALAKELNVPVMAL
EEEHHHHCCCCHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AQLNRKNEDRPDKKPILADLRDSGDIEQDADVVMFVHRPEMYDHNNAELKGYAEVLIRKQ
HHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
RNGALGDVALEYKGALTKFCEWTGPLPVIGTGSPVRKRGIAEYL
CCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHCCHHHCC
>Mature Secondary Structure 
TGPDDLPQARPLYAIEAEQAVLGGLLLDNNAIDRVGGLEPAHIYRHDHREILRAIVRLI
CCCCCCCCCCCEEEEEHHHHHHHHEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IAGKPADVVTVFESLQAAGKAVDVGGLSYLNSLAQNTPSAANIARYAEIVKDRALLRETL
HCCCCHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ATARKVQELVETPSAMKGTEILDRAQTMLANLAQVGVRREPKMLRELLAPFVEEVDNRAT
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHH
GAIESAIPTGLDALDQALNGGMRRGNLIIVAGRPSMGKTALTTDIGLNVATDYNVLLLSM
HHHHHHCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCEEECCCEEEEEEE
EMSDQEIVARALASRGGIPLSKLLGRMNHGDDHAWTNLTAGVVAIDQLNFAVDDSPALTL
CCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHEEEECCCEEECCCCCEEE
LDVRMKAKGHQRKYGLDLLIVDYVGLMTGGEEKMRTQQIGAYSRGLKALAKELNVPVMAL
EEEHHHHCCCCHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AQLNRKNEDRPDKKPILADLRDSGDIEQDADVVMFVHRPEMYDHNNAELKGYAEVLIRKQ
HHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
RNGALGDVALEYKGALTKFCEWTGPLPVIGTGSPVRKRGIAEYL
CCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]