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The map label for this gene is phaZ1 [H]

Identifier: 187929347

GI number: 187929347

Start: 2388618

End: 2389652

Strand: Direct

Name: phaZ1 [H]

Synonym: Rpic_2268

Alternate gene names: 187929347

Gene position: 2388618-2389652 (Clockwise)

Preceding gene: 187929345

Following gene: 187929351

Centisome position: 60.59

GC content: 67.05

Gene sequence:

>1035_bases
ATGAAATCCATCACCCGCCTGGTCGACGCCTTGACCACACCGGGCTTTGGGCGCCCGTCATGGCCGCCTAAACCGGTTAA
GCCGCTCAAGCCAACCACACGCGGCGAGAGCCTGCGCCGCGTACGCCCCACGCCCACCCAGGGCACCTTCGTCCGCGAAC
AGTTCATCTACGAACCCGAGTCGCCCGCCGTGGTGGCGCGCCGGCCGGAATACTGGCTCTACACACCGCCCACACGCGGC
AGCAAGCCCCCGGCACTGATGGTGATGCTGCATGGCTGCAAACAATCGGCGGAAGTGCTGGCGCATGGCACGCGCATGAA
CGCACTGGCAGACCGCGAGGGCTTTATCGTGCTGTATCCGGAGCAGCCGCGCCGCGCGCATCCGCAATGCTGCTGGCACT
GGTATGACCCGGAGCACGCTGGCGCGCGCGAGGCCGACGGCATTGCCCAACTGATCGAACAAACCCTGGCACAAACTGGC
GCAGACCCCTCGCGCGTGTATCTGGCAGGGTTGTCGGCCGGCGCGGGATTGGCCGGCCTGATGGCATTGCGTCATCCGAA
GTTGTTTGCCGCGTTGGCGCTACATTCGGGCCCGGCCTTGGGCGTGGCACGCTCGCCGGCGTCTGCATTGAACCTGATGC
GCCGCGGGGCACGCGTCGACGCCGTCGATGCGCTCTCCAGCGTGGTAAACGTGGCCGCCTATCCCGGCATGCCAACGATG
CTGTTGCACGGTCTGCGCGATGAGGCCGTCAACCCCACCAACCAAGCGCAGTTAGCCGCGCAGTTCCGCGCGCTGAATCA
TCTGGGCGATGGGCGCAACGTCTCACGCGAAACCACGCGCGGCGAAGGCTATGTCCTGCGCAACGACATACGCGACGGCG
AATGCATCGTCAGCCTGTGCCAACTCACCGCCACGGGCCACGCCTGGAGCGGCGGCGACCCGCGCTACGCCTTCCACGCG
CCGGGCCCGGACGCGACCTGGCTGTGGTGGCAATTCGCACGCCGGCACATTCGCGATACAGCGCTCGCGGCGTAA

Upstream 100 bases:

>100_bases
ACTGTCCTACCAATACCGAAACCCCTTGCGACCTCTCGGAGAGCACGCGCACCATCGTCCTCATCCGAGCAACTTTGCCT
CCGCCATGCACCGTAATTGG

Downstream 100 bases:

>100_bases
GCGGACGTTAGAGATCGATCGACTGCTGCACGGCGTTGCGCCCCAGCAATTGATCGATCAAGCCATAGCCGCGCGTGAGC
CCGGCGTCTTCGGCGGCTTC

Product: esterase, PHB depolymerase family

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MKSITRLVDALTTPGFGRPSWPPKPVKPLKPTTRGESLRRVRPTPTQGTFVREQFIYEPESPAVVARRPEYWLYTPPTRG
SKPPALMVMLHGCKQSAEVLAHGTRMNALADREGFIVLYPEQPRRAHPQCCWHWYDPEHAGAREADGIAQLIEQTLAQTG
ADPSRVYLAGLSAGAGLAGLMALRHPKLFAALALHSGPALGVARSPASALNLMRRGARVDAVDALSSVVNVAAYPGMPTM
LLHGLRDEAVNPTNQAQLAAQFRALNHLGDGRNVSRETTRGEGYVLRNDIRDGECIVSLCQLTATGHAWSGGDPRYAFHA
PGPDATWLWWQFARRHIRDTALAA

Sequences:

>Translated_344_residues
MKSITRLVDALTTPGFGRPSWPPKPVKPLKPTTRGESLRRVRPTPTQGTFVREQFIYEPESPAVVARRPEYWLYTPPTRG
SKPPALMVMLHGCKQSAEVLAHGTRMNALADREGFIVLYPEQPRRAHPQCCWHWYDPEHAGAREADGIAQLIEQTLAQTG
ADPSRVYLAGLSAGAGLAGLMALRHPKLFAALALHSGPALGVARSPASALNLMRRGARVDAVDALSSVVNVAAYPGMPTM
LLHGLRDEAVNPTNQAQLAAQFRALNHLGDGRNVSRETTRGEGYVLRNDIRDGECIVSLCQLTATGHAWSGGDPRYAFHA
PGPDATWLWWQFARRHIRDTALAA
>Mature_344_residues
MKSITRLVDALTTPGFGRPSWPPKPVKPLKPTTRGESLRRVRPTPTQGTFVREQFIYEPESPAVVARRPEYWLYTPPTRG
SKPPALMVMLHGCKQSAEVLAHGTRMNALADREGFIVLYPEQPRRAHPQCCWHWYDPEHAGAREADGIAQLIEQTLAQTG
ADPSRVYLAGLSAGAGLAGLMALRHPKLFAALALHSGPALGVARSPASALNLMRRGARVDAVDALSSVVNVAAYPGMPTM
LLHGLRDEAVNPTNQAQLAAQFRALNHLGDGRNVSRETTRGEGYVLRNDIRDGECIVSLCQLTATGHAWSGGDPRYAFHA
PGPDATWLWWQFARRHIRDTALAA

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 37589; Mature: 37589

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSITRLVDALTTPGFGRPSWPPKPVKPLKPTTRGESLRRVRPTPTQGTFVREQFIYEPE
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHEECCC
SPAVVARRPEYWLYTPPTRGSKPPALMVMLHGCKQSAEVLAHGTRMNALADREGFIVLYP
CCEEEEECCCEEEECCCCCCCCCCEEEEEEHHCCHHHHHHHCCCHHHHHHCCCCEEEECC
EQPRRAHPQCCWHWYDPEHAGAREADGIAQLIEQTLAQTGADPSRVYLAGLSAGAGLAGL
CCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
MALRHPKLFAALALHSGPALGVARSPASALNLMRRGARVDAVDALSSVVNVAAYPGMPTM
HHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHH
LLHGLRDEAVNPTNQAQLAAQFRALNHLGDGRNVSRETTRGEGYVLRNDIRDGECIVSLC
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHH
QLTATGHAWSGGDPRYAFHAPGPDATWLWWQFARRHIRDTALAA
HHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKSITRLVDALTTPGFGRPSWPPKPVKPLKPTTRGESLRRVRPTPTQGTFVREQFIYEPE
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHEECCC
SPAVVARRPEYWLYTPPTRGSKPPALMVMLHGCKQSAEVLAHGTRMNALADREGFIVLYP
CCEEEEECCCEEEECCCCCCCCCCEEEEEEHHCCHHHHHHHCCCHHHHHHCCCCEEEECC
EQPRRAHPQCCWHWYDPEHAGAREADGIAQLIEQTLAQTGADPSRVYLAGLSAGAGLAGL
CCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
MALRHPKLFAALALHSGPALGVARSPASALNLMRRGARVDAVDALSSVVNVAAYPGMPTM
HHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHH
LLHGLRDEAVNPTNQAQLAAQFRALNHLGDGRNVSRETTRGEGYVLRNDIRDGECIVSLC
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHH
QLTATGHAWSGGDPRYAFHAPGPDATWLWWQFARRHIRDTALAA
HHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]