Click here to switch to the map view.

The map label for this gene is guaD [H]

Identifier: 187929329

GI number: 187929329

Start: 2362004

End: 2363338

Strand: Direct

Name: guaD [H]

Synonym: Rpic_2250

Alternate gene names: 187929329

Gene position: 2362004-2363338 (Clockwise)

Preceding gene: 187929328

Following gene: 187929330

Centisome position: 59.91

GC content: 65.92

Gene sequence:

>1335_bases
ATGACCACGATGCCTCCGACTTCCAACACCGTTCACGCCTACCGCGGCCGCGTGCTGCATTTCCTGCACGATCCGCAGTA
TCGCGAGCGCGATGCCTATGAGTACTGGGAAGACGGCCTGCTCGTGGTCTTGGGCGGCAAGGTGATCAAGGCCGGCGACT
ATGCCGCGCTACGTGAAGACCTGCCGGCCGGCGCGCAATTGCACGACTACAGCGGCAAGCTGATCGTCCCGGGCTTCATC
GACACTCATATCCACTTCCCCCAGACGGACATCATCGCGTCGCCGTCGCCGGGTCTGCTGCATTGGCTGGAGACGTACAC
CTTCCCGGAAGAGCGCCGCTTCGAAAGCGAGCAGTACGCCGCCGGCGTCGCCTCGTTCTTCCTCGACGAACTGCTGCGCA
ACGGCACGACCAGCGCGATGGTGTGGAGCACCGTGCATCGCGGGTCTGCCGAGACGCTGTTCACGCAAGCCCAGCAACGC
GGCATGCGCATGATCACGGGCAAGGTGATGATGGACCGCAACTGCCCGGAATACCTGCGCGACACGGCGGAATCCGGCGC
GCGCGACACGGCTGACCTGATCTCGCGCTGGCACGGCAAGGACCGCCTGGCTTACGCCATCACGCCGCGATTCGCGCCCA
CGTCGAGCGAAGCCCAGCTGCAGGCCTGCGCCGAACTCGCCAAGCAATACAAGGACGTCTTCATCCAGACGCACGTCGCC
GAGAACCCCGACGAAGTGAAATGGGTGGCTGACCTGTTCCCAGACGCGCGCAGCTATCTGGACGTCTACGACCGCTACGG
CCTGCTGCGCAAAGGCGCCTTTTACGGCCACGCCATCTGGCTGGACGACGGTGACCGCCAGCGCATAGCCGAATCCGGCG
CAGCCGTCGCGCACTGCCCCACCTCCAACCTGTTCCTGGGCAGCGGCCTGTACAACTTCCACAAGAACGACGCACACCGC
CTGGCACTCACGCTGGCGACCGACGTGGGCGGCGGCAGCTCGTTCTCGATGCTCCGCACGATGGGCACGGCGCATAAGGT
CGCGCGCATGGGCGGTTATCACCTGACTGCGCTGCGGATGTTCTACCTGGCCACGCGCGGCGCCGCTGAGGCTCTGGGCT
GGGAAGACCGCATCGGCAGCTTCGTGCCGGGCGCGGAAGCCGACTTCATCGTGCTGGACCCGGCAGCCACGCCGCTGCTG
GCCCGCCGCAATGCGCGCTCGGAAACGCTGGAGGAACTGCTGTTCTCATTGGCGCTGCTGGGCGAGGATCGCGCCGTGGC
TGCCACATACATCCAGGGCGAGCCCGCCAAATTCGCGGTTGGCGCCAACGCCTGA

Upstream 100 bases:

>100_bases
AGCTTCGAGGCGTCATTCCTGCCCGAACAGGACAAAGCCCTGTGGCTCGCCAAGGTCGACGACCACTGGAAAGCCGCACA
CTAAAAATCCACGAGACGAT

Downstream 100 bases:

>100_bases
TCCATTTATAATCCTCGCTTCTATTGGGGAGTAGCCGCCTGGGGTCGATACCCCGGGGGCCACGTCAACAGACTTGACCG
CCGTCGCAACGACAGGTGGT

Product: guanine deaminase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MTTMPPTSNTVHAYRGRVLHFLHDPQYRERDAYEYWEDGLLVVLGGKVIKAGDYAALREDLPAGAQLHDYSGKLIVPGFI
DTHIHFPQTDIIASPSPGLLHWLETYTFPEERRFESEQYAAGVASFFLDELLRNGTTSAMVWSTVHRGSAETLFTQAQQR
GMRMITGKVMMDRNCPEYLRDTAESGARDTADLISRWHGKDRLAYAITPRFAPTSSEAQLQACAELAKQYKDVFIQTHVA
ENPDEVKWVADLFPDARSYLDVYDRYGLLRKGAFYGHAIWLDDGDRQRIAESGAAVAHCPTSNLFLGSGLYNFHKNDAHR
LALTLATDVGGGSSFSMLRTMGTAHKVARMGGYHLTALRMFYLATRGAAEALGWEDRIGSFVPGAEADFIVLDPAATPLL
ARRNARSETLEELLFSLALLGEDRAVAATYIQGEPAKFAVGANA

Sequences:

>Translated_444_residues
MTTMPPTSNTVHAYRGRVLHFLHDPQYRERDAYEYWEDGLLVVLGGKVIKAGDYAALREDLPAGAQLHDYSGKLIVPGFI
DTHIHFPQTDIIASPSPGLLHWLETYTFPEERRFESEQYAAGVASFFLDELLRNGTTSAMVWSTVHRGSAETLFTQAQQR
GMRMITGKVMMDRNCPEYLRDTAESGARDTADLISRWHGKDRLAYAITPRFAPTSSEAQLQACAELAKQYKDVFIQTHVA
ENPDEVKWVADLFPDARSYLDVYDRYGLLRKGAFYGHAIWLDDGDRQRIAESGAAVAHCPTSNLFLGSGLYNFHKNDAHR
LALTLATDVGGGSSFSMLRTMGTAHKVARMGGYHLTALRMFYLATRGAAEALGWEDRIGSFVPGAEADFIVLDPAATPLL
ARRNARSETLEELLFSLALLGEDRAVAATYIQGEPAKFAVGANA
>Mature_443_residues
TTMPPTSNTVHAYRGRVLHFLHDPQYRERDAYEYWEDGLLVVLGGKVIKAGDYAALREDLPAGAQLHDYSGKLIVPGFID
THIHFPQTDIIASPSPGLLHWLETYTFPEERRFESEQYAAGVASFFLDELLRNGTTSAMVWSTVHRGSAETLFTQAQQRG
MRMITGKVMMDRNCPEYLRDTAESGARDTADLISRWHGKDRLAYAITPRFAPTSSEAQLQACAELAKQYKDVFIQTHVAE
NPDEVKWVADLFPDARSYLDVYDRYGLLRKGAFYGHAIWLDDGDRQRIAESGAAVAHCPTSNLFLGSGLYNFHKNDAHRL
ALTLATDVGGGSSFSMLRTMGTAHKVARMGGYHLTALRMFYLATRGAAEALGWEDRIGSFVPGAEADFIVLDPAATPLLA
RRNARSETLEELLFSLALLGEDRAVAATYIQGEPAKFAVGANA

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=376, Percent_Identity=35.1063829787234, Blast_Score=215, Evalue=7e-56,
Organism=Escherichia coli, GI1789249, Length=402, Percent_Identity=47.7611940298507, Blast_Score=414, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6319963, Length=332, Percent_Identity=34.3373493975904, Blast_Score=188, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24643849, Length=450, Percent_Identity=33.5555555555556, Blast_Score=209, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 49205; Mature: 49074

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTMPPTSNTVHAYRGRVLHFLHDPQYRERDAYEYWEDGLLVVLGGKVIKAGDYAALRED
CCCCCCCCCCHHHHHCCEEEEECCCCCCCCCHHHHHHCCEEEEECCEEEECCCHHHHHHC
LPAGAQLHDYSGKLIVPGFIDTHIHFPQTDIIASPSPGLLHWLETYTFPEERRFESEQYA
CCCCCEEECCCCCEEECCEEECCEECCCCCCCCCCCCHHHHHHHHCCCCHHHHCCHHHHH
AGVASFFLDELLRNGTTSAMVWSTVHRGSAETLFTQAQQRGMRMITGKVMMDRNCPEYLR
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCCCEEEECEEEECCCCHHHHH
DTAESGARDTADLISRWHGKDRLAYAITPRFAPTSSEAQLQACAELAKQYKDVFIQTHVA
HHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECC
ENPDEVKWVADLFPDARSYLDVYDRYGLLRKGAFYGHAIWLDDGDRQRIAESGAAVAHCP
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHCCCEEEECC
TSNLFLGSGLYNFHKNDAHRLALTLATDVGGGSSFSMLRTMGTAHKVARMGGYHLTALRM
CCCEEECCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
FYLATRGAAEALGWEDRIGSFVPGAEADFIVLDPAATPLLARRNARSETLEELLFSLALL
HHHHHCCHHHHCCCHHHCCCCCCCCCCCEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHH
GEDRAVAATYIQGEPAKFAVGANA
CCCCEEEEEEECCCCCEEEECCCC
>Mature Secondary Structure 
TTMPPTSNTVHAYRGRVLHFLHDPQYRERDAYEYWEDGLLVVLGGKVIKAGDYAALRED
CCCCCCCCCHHHHHCCEEEEECCCCCCCCCHHHHHHCCEEEEECCEEEECCCHHHHHHC
LPAGAQLHDYSGKLIVPGFIDTHIHFPQTDIIASPSPGLLHWLETYTFPEERRFESEQYA
CCCCCEEECCCCCEEECCEEECCEECCCCCCCCCCCCHHHHHHHHCCCCHHHHCCHHHHH
AGVASFFLDELLRNGTTSAMVWSTVHRGSAETLFTQAQQRGMRMITGKVMMDRNCPEYLR
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCCCEEEECEEEECCCCHHHHH
DTAESGARDTADLISRWHGKDRLAYAITPRFAPTSSEAQLQACAELAKQYKDVFIQTHVA
HHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECC
ENPDEVKWVADLFPDARSYLDVYDRYGLLRKGAFYGHAIWLDDGDRQRIAESGAAVAHCP
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHCCCEEEECC
TSNLFLGSGLYNFHKNDAHRLALTLATDVGGGSSFSMLRTMGTAHKVARMGGYHLTALRM
CCCEEECCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
FYLATRGAAEALGWEDRIGSFVPGAEADFIVLDPAATPLLARRNARSETLEELLFSLALL
HHHHHCCHHHHCCCHHHCCCCCCCCCCCEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHH
GEDRAVAATYIQGEPAKFAVGANA
CCCCEEEEEEECCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 10913105 [H]