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The map label for this gene is leuC [H]

Identifier: 187929224

GI number: 187929224

Start: 2256857

End: 2258266

Strand: Reverse

Name: leuC [H]

Synonym: Rpic_2145

Alternate gene names: 187929224

Gene position: 2258266-2256857 (Counterclockwise)

Preceding gene: 187929225

Following gene: 187929223

Centisome position: 57.28

GC content: 64.26

Gene sequence:

>1410_bases
ATGGCCAAGACGCTCTACGACAAACTTTGGGACGATCACGTCGTTCATACCGAAGAAGACGGCACGACCGTCCTCTACAT
CGATCGTCAACTGCTGCATGAAGTGACGAGCCCGCAGGCATTCGAGGGCCTGAAGTTAGCCGAGCGTCCGGTGTGGCGCA
TCAGCGCAAACCTGGCCGTGTCGGATCACAACGTGCCGACCACCGATCGTTCGCACGGCATTGCCGATCCGGTTTCGAAG
CTGCAAGTCGATACGCTTGATGCCAACTGCGACAGCTACGGCATCACGCAATTCAAGATGAACGACAAGCGCCAGGGCAT
CGTGCACGTGATCGGGCCGGAGCAGGGCGCGACGCTGCCGGGCATGACGGTGGTCTGCGGTGATTCGCACACCAGCACGC
ACGGTGCGTTCGGCGCGCTCGCGCACGGCATCGGCACCTCGGAAGTCGAGCACGTGCTGGCCACGCAAACGCTGCTCACC
AAGAAGAGCAAGAACATGCTGGTGAAGGTGGAAGGTACGCTGCCTCGCGGCTGCACGGCCAAGGACATCGTGCTCGCCAT
CATCGGCAAGATCGGTACGGCCGGTGGCACGGGCTACGCCATGGAATTCGGCGGCTCGGCCATCCGCGCGCTGTCGATGG
AAGGCCGCATGACGGTGTGCAACATGGCAATCGAGGCAGGCGCCCGCGCCGGCATCGTCGCCGTGGACGATGTCACGCTG
GAGTACATCAAGGGCCGTCCGTTCGCGCCGCAAGGCGTGGAGTGGGAGCAGGCCGTCGCCTACTGGCGCACGCTGCATTC
CGACGAGGGTGCGCACTTCGACCAGGTGATCGAACTGCGTGCTGAAGACATTCGCCCGCAGGTGAGCTGGGGGACATCGC
CGGAAATGGTGGTCAGCATTGAAGACCGCGTGCCGGATCCCGACAAAGAGAAAGACCCGAACAAGCGCAACGCCATGGAG
CGCGCGCTCGAGTACATGGGCCTGCAGCCGAACGTCGCCATCAGCGACATCAACATCGACAAGGTGTTCATCGGCTCGTG
CACCAACAGCCGCATCGAAGACATGCGCGCCGCGGCGTGGGTCGTGCAGAAGCTGGGCAAGCGCATTGCGTCGAACGTGA
AGCTGGCGATGGTCGTGCCGGGCTCGGGCCTCGTGAAGGAGCAGGCCGAGCGCGAAGGGCTCGACAAGATCTTCAAGGCG
GCGGGCTTTGAGTGGCGCGAGCCGGGTTGCTCGATGTGTCTCGCCATGAACGCTGACCGCCTCGAGCCGGGCGAACGCTG
CGCGTCCACGTCCAACCGCAACTTCGAAGGCCGTCAGGGCGCGGGCGGACGCACCCACCTGGTAAGCCCGGCGATGGCCG
CTGCGGCCGCGCTGGAAGGCCACTTCGTCGACGTGCGCAAGCTCGCTTGA

Upstream 100 bases:

>100_bases
CGGTGCCCGATGCACCGGGTGGCATAATCGATCACTCCCCCACGAAACTGCTCGCGCAACGGCCCTGATTTCAGATGGCG
CGTTGTGTACGGATCGCATC

Downstream 100 bases:

>100_bases
GCGGCAGGATAGAGAGAGCAATCATGGAACAATTCACCATCCACACCGGCCTCGTGGCGCCGCTCGACCGCGAGAATGTC
GACACCGACGCCATCATCCC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MAKTLYDKLWDDHVVHTEEDGTTVLYIDRQLLHEVTSPQAFEGLKLAERPVWRISANLAVSDHNVPTTDRSHGIADPVSK
LQVDTLDANCDSYGITQFKMNDKRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLT
KKSKNMLVKVEGTLPRGCTAKDIVLAIIGKIGTAGGTGYAMEFGGSAIRALSMEGRMTVCNMAIEAGARAGIVAVDDVTL
EYIKGRPFAPQGVEWEQAVAYWRTLHSDEGAHFDQVIELRAEDIRPQVSWGTSPEMVVSIEDRVPDPDKEKDPNKRNAME
RALEYMGLQPNVAISDINIDKVFIGSCTNSRIEDMRAAAWVVQKLGKRIASNVKLAMVVPGSGLVKEQAEREGLDKIFKA
AGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAALEGHFVDVRKLA

Sequences:

>Translated_469_residues
MAKTLYDKLWDDHVVHTEEDGTTVLYIDRQLLHEVTSPQAFEGLKLAERPVWRISANLAVSDHNVPTTDRSHGIADPVSK
LQVDTLDANCDSYGITQFKMNDKRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLT
KKSKNMLVKVEGTLPRGCTAKDIVLAIIGKIGTAGGTGYAMEFGGSAIRALSMEGRMTVCNMAIEAGARAGIVAVDDVTL
EYIKGRPFAPQGVEWEQAVAYWRTLHSDEGAHFDQVIELRAEDIRPQVSWGTSPEMVVSIEDRVPDPDKEKDPNKRNAME
RALEYMGLQPNVAISDINIDKVFIGSCTNSRIEDMRAAAWVVQKLGKRIASNVKLAMVVPGSGLVKEQAEREGLDKIFKA
AGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAALEGHFVDVRKLA
>Mature_468_residues
AKTLYDKLWDDHVVHTEEDGTTVLYIDRQLLHEVTSPQAFEGLKLAERPVWRISANLAVSDHNVPTTDRSHGIADPVSKL
QVDTLDANCDSYGITQFKMNDKRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLTK
KSKNMLVKVEGTLPRGCTAKDIVLAIIGKIGTAGGTGYAMEFGGSAIRALSMEGRMTVCNMAIEAGARAGIVAVDDVTLE
YIKGRPFAPQGVEWEQAVAYWRTLHSDEGAHFDQVIELRAEDIRPQVSWGTSPEMVVSIEDRVPDPDKEKDPNKRNAMER
ALEYMGLQPNVAISDINIDKVFIGSCTNSRIEDMRAAAWVVQKLGKRIASNVKLAMVVPGSGLVKEQAEREGLDKIFKAA
GFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAALEGHFVDVRKLA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=363, Percent_Identity=28.099173553719, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI41352693, Length=368, Percent_Identity=27.445652173913, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI8659555, Length=402, Percent_Identity=27.1144278606965, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1786259, Length=468, Percent_Identity=63.034188034188, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI1787531, Length=367, Percent_Identity=28.6103542234332, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25149337, Length=365, Percent_Identity=31.5068493150685, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32564738, Length=378, Percent_Identity=31.2169312169312, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI25149342, Length=303, Percent_Identity=31.023102310231, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17568399, Length=400, Percent_Identity=27, Blast_Score=104, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=63.9830508474576, Blast_Score=612, Evalue=1e-176,
Organism=Saccharomyces cerevisiae, GI6320440, Length=355, Percent_Identity=27.0422535211268, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6322261, Length=477, Percent_Identity=27.2536687631027, Blast_Score=130, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6323335, Length=364, Percent_Identity=28.021978021978, Blast_Score=130, Evalue=6e-31,
Organism=Drosophila melanogaster, GI281365315, Length=394, Percent_Identity=26.6497461928934, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI17864292, Length=394, Percent_Identity=26.6497461928934, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI161076999, Length=394, Percent_Identity=26.6497461928934, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI28571643, Length=400, Percent_Identity=27.25, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24645686, Length=381, Percent_Identity=27.0341207349081, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17137564, Length=381, Percent_Identity=25.4593175853018, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50841; Mature: 50710

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTLYDKLWDDHVVHTEEDGTTVLYIDRQLLHEVTSPQAFEGLKLAERPVWRISANLAV
CCCHHHHHHCCCCEEEECCCCCEEEEECHHHHHHHCCCCHHCCCHHHCCCEEEEEEEEEE
SDHNVPTTDRSHGIADPVSKLQVDTLDANCDSYGITQFKMNDKRQGIVHVIGPEQGATLP
ECCCCCCCCCCCCCCCCHHHEEEEEECCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCC
GMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLTKKSKNMLVKVEGTLPRGCTA
CEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCH
KDIVLAIIGKIGTAGGTGYAMEFGGSAIRALSMEGRMTVCNMAIEAGARAGIVAVDDVTL
HHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCEEEEHHHHHCCCCCCEEEECCHHH
EYIKGRPFAPQGVEWEQAVAYWRTLHSDEGAHFDQVIELRAEDIRPQVSWGTSPEMVVSI
HHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEE
EDRVPDPDKEKDPNKRNAMERALEYMGLQPNVAISDINIDKVFIGSCTNSRIEDMRAAAW
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHH
VVQKLGKRIASNVKLAMVVPGSGLVKEQAEREGLDKIFKAAGFEWREPGCSMCLAMNADR
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCC
LEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAALEGHFVDVRKLA
CCCHHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCEEEHEECC
>Mature Secondary Structure 
AKTLYDKLWDDHVVHTEEDGTTVLYIDRQLLHEVTSPQAFEGLKLAERPVWRISANLAV
CCHHHHHHCCCCEEEECCCCCEEEEECHHHHHHHCCCCHHCCCHHHCCCEEEEEEEEEE
SDHNVPTTDRSHGIADPVSKLQVDTLDANCDSYGITQFKMNDKRQGIVHVIGPEQGATLP
ECCCCCCCCCCCCCCCCHHHEEEEEECCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCC
GMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLTKKSKNMLVKVEGTLPRGCTA
CEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCH
KDIVLAIIGKIGTAGGTGYAMEFGGSAIRALSMEGRMTVCNMAIEAGARAGIVAVDDVTL
HHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCEEEEHHHHHCCCCCCEEEECCHHH
EYIKGRPFAPQGVEWEQAVAYWRTLHSDEGAHFDQVIELRAEDIRPQVSWGTSPEMVVSI
HHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEE
EDRVPDPDKEKDPNKRNAMERALEYMGLQPNVAISDINIDKVFIGSCTNSRIEDMRAAAW
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHH
VVQKLGKRIASNVKLAMVVPGSGLVKEQAEREGLDKIFKAAGFEWREPGCSMCLAMNADR
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCC
LEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAALEGHFVDVRKLA
CCCHHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCEEEHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]