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The map label for this gene is rnhA [H]

Identifier: 187929120

GI number: 187929120

Start: 2151391

End: 2151837

Strand: Direct

Name: rnhA [H]

Synonym: Rpic_2041

Alternate gene names: 187929120

Gene position: 2151391-2151837 (Clockwise)

Preceding gene: 187929119

Following gene: 187929125

Centisome position: 54.57

GC content: 62.86

Gene sequence:

>447_bases
ATGCAGGAAGTCACCGTTTATTCCGATGGCGCGTGCAAGGGCAACCCCGGCCTTGGTGGCTGGGGCACCGTGCTCGTCTC
GGGCAGCCACGAAAAAGAACTCTTTGGCGGCGAAACGCTAACCACCAACAACCGCATGGAGCTGATGGCCGTGATCGAAG
CATTCCGCGCGCTGAAAAGGCCATGCCGGGTGCAGGTTTATACCGATTCGCAGTACGTCCAGAAAGGCATCAGCGAATGG
TTGGCCGGCTGGAAGGCGCGCGGGTGGAAGACGGCCGACAAGAAGCCGGTCAAGAACGACGACCTCTGGCGCACGCTCGA
CGAACTGGTCGCCGGACATGAGGTGAGCTGGCATTGGGTCAAGGGCCATGCCGGCCACCCCGGCAACGAGCGCGCCGATG
CGCTGGCCAACAAAGGCGTCGAGATGGCGCGCCAGGCGAAAGCCTGA

Upstream 100 bases:

>100_bases
GGGATCACATTCGACCAAGGCGCCATCGGCCACGGCGCGTGACGGCAAATCGGGCGCGTGAGCGCGACCCTTCCACTCAC
TGATCACCCATCGTTTCTCA

Downstream 100 bases:

>100_bases
CGCAGCCGGGCGCACGCTCACCTGCCCGCGCAGCGCTGCGTCGGGATCTGCGGCGCATTCGCGGCGGTGGTCGTGACGTA
CTGCGGCTTGCCATCCTGCG

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MQEVTVYSDGACKGNPGLGGWGTVLVSGSHEKELFGGETLTTNNRMELMAVIEAFRALKRPCRVQVYTDSQYVQKGISEW
LAGWKARGWKTADKKPVKNDDLWRTLDELVAGHEVSWHWVKGHAGHPGNERADALANKGVEMARQAKA

Sequences:

>Translated_148_residues
MQEVTVYSDGACKGNPGLGGWGTVLVSGSHEKELFGGETLTTNNRMELMAVIEAFRALKRPCRVQVYTDSQYVQKGISEW
LAGWKARGWKTADKKPVKNDDLWRTLDELVAGHEVSWHWVKGHAGHPGNERADALANKGVEMARQAKA
>Mature_148_residues
MQEVTVYSDGACKGNPGLGGWGTVLVSGSHEKELFGGETLTTNNRMELMAVIEAFRALKRPCRVQVYTDSQYVQKGISEW
LAGWKARGWKTADKKPVKNDDLWRTLDELVAGHEVSWHWVKGHAGHPGNERADALANKGVEMARQAKA

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=151, Percent_Identity=36.4238410596026, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1786408, Length=136, Percent_Identity=58.0882352941177, Blast_Score=172, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI115532542, Length=142, Percent_Identity=40.1408450704225, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17137476, Length=152, Percent_Identity=34.8684210526316, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI45552509, Length=152, Percent_Identity=34.8684210526316, Blast_Score=87, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 16377; Mature: 16377

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEVTVYSDGACKGNPGLGGWGTVLVSGSHEKELFGGETLTTNNRMELMAVIEAFRALKR
CCCEEEEECCCCCCCCCCCCCEEEEECCCCCCHHCCCCEECCCCHHHHHHHHHHHHHHCC
PCRVQVYTDSQYVQKGISEWLAGWKARGWKTADKKPVKNDDLWRTLDELVAGHEVSWHWV
CCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEE
KGHAGHPGNERADALANKGVEMARQAKA
ECCCCCCCCHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure
MQEVTVYSDGACKGNPGLGGWGTVLVSGSHEKELFGGETLTTNNRMELMAVIEAFRALKR
CCCEEEEECCCCCCCCCCCCCEEEEECCCCCCHHCCCCEECCCCHHHHHHHHHHHHHHCC
PCRVQVYTDSQYVQKGISEWLAGWKARGWKTADKKPVKNDDLWRTLDELVAGHEVSWHWV
CCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEE
KGHAGHPGNERADALANKGVEMARQAKA
ECCCCCCCCHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]