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The map label for this gene is mdeA [H]

Identifier: 187929071

GI number: 187929071

Start: 2101103

End: 2102485

Strand: Direct

Name: mdeA [H]

Synonym: Rpic_1991

Alternate gene names: 187929071

Gene position: 2101103-2102485 (Clockwise)

Preceding gene: 187929066

Following gene: 187929072

Centisome position: 53.29

GC content: 66.96

Gene sequence:

>1383_bases
ATGCCGCGTTCAAACCTGTTCCCGCGCCCTTTCCGGGTGCCTGAATCCATGTCCAAAGCCCGCTTCGACACGCTGGCCCT
GCATGCCGGCGCCGCGCCCGACCCGGCCACCGGCGCACGCGCCACGCCGATTCACCTGAGCACGTCCTTTGTGTTCCGCG
ACAGCGAACATGCGGCGTCATTGTTCAATATGGAACGCGCGGGCCACGTCTACTCGCGCATCTCCAACCCGACCGTTGCC
GTGTTCGAAGAGCGCATGGCCGCGCTGGAAAACGGCGTAGGCGCCATCGCCACGGCTTCGGGGCAGGCGGCGCTGCATCT
GGCCATCGTCACGCTGATGGGTGCCGGCTCGCATATCGTCGCGTCCTCGGCGCTGTATGGCGGCTCGCACAACTTGCTGC
ACTACACGCTGCGCCGCTTCGGTATCGAGACAACCTTCGTCAAACCCGGCGACATCGATGGCTGGCGCGCTGCCATCCGC
CCGAACACGCGGCTGCTGTTTGGCGAGACGCTCGGCAACCCGGGGCTGGATGTGCTCGACATCCCGACCATTGCATCGAT
TGCGCATGACGCGGGCGTGCCCCTGCTGGTCGACTCGACCTTCACCACGCCTTGGCTGCTGCAGGCGTTCGCGCATGGCG
CAGATCTCGTCTATCACTCGGCCACCAAGTTCCTGGGCGGGCATGGCACGACCATCGGCGGCGTGCTTGTCGACGGGGGC
ACATTCGACTACGTGGCCGCCGGTAAGCACCCCGAACTGACCGAGCCGTACGCGGGCTTTCACGACATGGTGTTTGCCGA
GGAGAGCACCGTCGCGCCCTTCCTGCTGCGAGCGCGCCGCGAGGGCCTGCGCGACTTCGGCGCCTGCATGAACCCGATGG
CCGCATGGCAACTGCTGCAAGGCATCGAGACACTGCCGCTGCGCATGGCGCGACATGTGGACAACGCCCGCCGCATCGTC
GCCTTCCTGGCGAGCCACCCAATGGTGGCGTCGGTCGCCTACCCGGAGCTGGAATCGCACCCCGATCACGCACTGGCCAA
GCGCCTGCTGCCGCGTGGCAGCGGCGCGGTGTTCAGCTTCGATCTGCGCGGTGATCGGCGTGCCGGCCAGCAGTTCATCG
AATCGCTCGGACTGTTCTCGCACCTTGCCAACGTGGGCGATGCGCGCTCGCTGGTCATCCACCCCGCGTCGACAACGCAC
TTCCGCATGGACGCCGACGCGCTGGCCGCAGCCGGCATCAGCGAAGGCACGATCCGCCTCTCCATCGGCCTTGAAGACGC
CGACGACCTGATCGACGATCTCAAGCGCGGCCTGAAAGCTGCCGAGCGCGCGATGTCGGCCGTGCCGGGCAAAGCCGCCG
TCAAAGCAACGGGAGCCCGGTGA

Upstream 100 bases:

>100_bases
CGGATCGCTTCGACAAGCATGCAGCGCAGAAAAAACCTGTCAAAAACCGCGCCCGCTCGGCGTTTGCCCGGAATGCGCTG
CAACAGGGAGCAGTGCTAGC

Downstream 100 bases:

>100_bases
TGGAATGGACCGTACAAAGCGAGCGCGCCTACGCCTACACCGGCGGCAAACCGTTCAACGCCGAACTGCCCTGCGTCGTC
TTCGTGCATGGCGCGCAGAA

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MPRSNLFPRPFRVPESMSKARFDTLALHAGAAPDPATGARATPIHLSTSFVFRDSEHAASLFNMERAGHVYSRISNPTVA
VFEERMAALENGVGAIATASGQAALHLAIVTLMGAGSHIVASSALYGGSHNLLHYTLRRFGIETTFVKPGDIDGWRAAIR
PNTRLLFGETLGNPGLDVLDIPTIASIAHDAGVPLLVDSTFTTPWLLQAFAHGADLVYHSATKFLGGHGTTIGGVLVDGG
TFDYVAAGKHPELTEPYAGFHDMVFAEESTVAPFLLRARREGLRDFGACMNPMAAWQLLQGIETLPLRMARHVDNARRIV
AFLASHPMVASVAYPELESHPDHALAKRLLPRGSGAVFSFDLRGDRRAGQQFIESLGLFSHLANVGDARSLVIHPASTTH
FRMDADALAAAGISEGTIRLSIGLEDADDLIDDLKRGLKAAERAMSAVPGKAAVKATGAR

Sequences:

>Translated_460_residues
MPRSNLFPRPFRVPESMSKARFDTLALHAGAAPDPATGARATPIHLSTSFVFRDSEHAASLFNMERAGHVYSRISNPTVA
VFEERMAALENGVGAIATASGQAALHLAIVTLMGAGSHIVASSALYGGSHNLLHYTLRRFGIETTFVKPGDIDGWRAAIR
PNTRLLFGETLGNPGLDVLDIPTIASIAHDAGVPLLVDSTFTTPWLLQAFAHGADLVYHSATKFLGGHGTTIGGVLVDGG
TFDYVAAGKHPELTEPYAGFHDMVFAEESTVAPFLLRARREGLRDFGACMNPMAAWQLLQGIETLPLRMARHVDNARRIV
AFLASHPMVASVAYPELESHPDHALAKRLLPRGSGAVFSFDLRGDRRAGQQFIESLGLFSHLANVGDARSLVIHPASTTH
FRMDADALAAAGISEGTIRLSIGLEDADDLIDDLKRGLKAAERAMSAVPGKAAVKATGAR
>Mature_459_residues
PRSNLFPRPFRVPESMSKARFDTLALHAGAAPDPATGARATPIHLSTSFVFRDSEHAASLFNMERAGHVYSRISNPTVAV
FEERMAALENGVGAIATASGQAALHLAIVTLMGAGSHIVASSALYGGSHNLLHYTLRRFGIETTFVKPGDIDGWRAAIRP
NTRLLFGETLGNPGLDVLDIPTIASIAHDAGVPLLVDSTFTTPWLLQAFAHGADLVYHSATKFLGGHGTTIGGVLVDGGT
FDYVAAGKHPELTEPYAGFHDMVFAEESTVAPFLLRARREGLRDFGACMNPMAAWQLLQGIETLPLRMARHVDNARRIVA
FLASHPMVASVAYPELESHPDHALAKRLLPRGSGAVFSFDLRGDRRAGQQFIESLGLFSHLANVGDARSLVIHPASTTHF
RMDADALAAAGISEGTIRLSIGLEDADDLIDDLKRGLKAAERAMSAVPGKAAVKATGAR

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=426, Percent_Identity=34.2723004694836, Blast_Score=186, Evalue=6e-47,
Organism=Homo sapiens, GI299473759, Length=426, Percent_Identity=31.4553990610329, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI25453487, Length=425, Percent_Identity=30.1176470588235, Blast_Score=135, Evalue=9e-32,
Organism=Escherichia coli, GI1790375, Length=420, Percent_Identity=34.7619047619048, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1789383, Length=436, Percent_Identity=26.605504587156, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71980708, Length=434, Percent_Identity=30.184331797235, Blast_Score=201, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI17538055, Length=425, Percent_Identity=30.5882352941176, Blast_Score=179, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17560056, Length=424, Percent_Identity=30.8962264150943, Blast_Score=174, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6323333, Length=436, Percent_Identity=42.8899082568807, Blast_Score=308, Evalue=9e-85,
Organism=Saccharomyces cerevisiae, GI6319307, Length=428, Percent_Identity=33.1775700934579, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321170, Length=356, Percent_Identity=26.9662921348315, Blast_Score=126, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6321254, Length=450, Percent_Identity=27.5555555555556, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321904, Length=429, Percent_Identity=27.7389277389277, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19922554, Length=427, Percent_Identity=32.3185011709602, Blast_Score=220, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 49085; Mature: 48954

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRSNLFPRPFRVPESMSKARFDTLALHAGAAPDPATGARATPIHLSTSFVFRDSEHAAS
CCCCCCCCCCCCCCHHHHHHHHHHHEEECCCCCCCCCCCCCCEEEEEEEEEEECCHHHHH
LFNMERAGHVYSRISNPTVAVFEERMAALENGVGAIATASGQAALHLAIVTLMGAGSHIV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCHHH
ASSALYGGSHNLLHYTLRRFGIETTFVKPGDIDGWRAAIRPNTRLLFGETLGNPGLDVLD
HHHHHCCCCCHHHHHHHHHHCCCEEEECCCCCCCCEEEECCCCEEEEECCCCCCCCCEEE
IPTIASIAHDAGVPLLVDSTFTTPWLLQAFAHGADLVYHSATKFLGGHGTTIGGVLVDGG
CCHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCEECEEEECCC
TFDYVAAGKHPELTEPYAGFHDMVFAEESTVAPFLLRARREGLRDFGACMNPMAAWQLLQ
CEEEEECCCCCCCCCCCCCHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
GIETLPLRMARHVDNARRIVAFLASHPMVASVAYPELESHPDHALAKRLLPRGSGAVFSF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCEEEE
DLRGDRRAGQQFIESLGLFSHLANVGDARSLVIHPASTTHFRMDADALAAAGISEGTIRL
ECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECHHHHHHCCCCCCEEEE
SIGLEDADDLIDDLKRGLKAAERAMSAVPGKAAVKATGAR
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
>Mature Secondary Structure 
PRSNLFPRPFRVPESMSKARFDTLALHAGAAPDPATGARATPIHLSTSFVFRDSEHAAS
CCCCCCCCCCCCCHHHHHHHHHHHEEECCCCCCCCCCCCCCEEEEEEEEEEECCHHHHH
LFNMERAGHVYSRISNPTVAVFEERMAALENGVGAIATASGQAALHLAIVTLMGAGSHIV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCHHH
ASSALYGGSHNLLHYTLRRFGIETTFVKPGDIDGWRAAIRPNTRLLFGETLGNPGLDVLD
HHHHHCCCCCHHHHHHHHHHCCCEEEECCCCCCCCEEEECCCCEEEEECCCCCCCCCEEE
IPTIASIAHDAGVPLLVDSTFTTPWLLQAFAHGADLVYHSATKFLGGHGTTIGGVLVDGG
CCHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCEECEEEECCC
TFDYVAAGKHPELTEPYAGFHDMVFAEESTVAPFLLRARREGLRDFGACMNPMAAWQLLQ
CEEEEECCCCCCCCCCCCCHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
GIETLPLRMARHVDNARRIVAFLASHPMVASVAYPELESHPDHALAKRLLPRGSGAVFSF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCEEEE
DLRGDRRAGQQFIESLGLFSHLANVGDARSLVIHPASTTHFRMDADALAAAGISEGTIRL
ECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECHHHHHHCCCCCCEEEE
SIGLEDADDLIDDLKRGLKAAERAMSAVPGKAAVKATGAR
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]