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The map label for this gene is hpcB [H]

Identifier: 187929037

GI number: 187929037

Start: 2067103

End: 2067972

Strand: Reverse

Name: hpcB [H]

Synonym: Rpic_1957

Alternate gene names: 187929037

Gene position: 2067972-2067103 (Counterclockwise)

Preceding gene: 187929038

Following gene: 187929036

Centisome position: 52.45

GC content: 61.26

Gene sequence:

>870_bases
ATGGGCAAGCTGGCCTTGGCTGCGAAGATCACCCACGTGCCGTCGATGTACCTGTCGGAGCTGCCGGGCAAGCATCATGG
CTGCCGCGAGGCGGCCATCGAGGGCCACCGCATCATCGGACAGCGTTGCCGCGATATGGGCGTGGACACCATCGTCGTGT
CCGACGTGCACTGGCTCGTCAACGCCGGTTACCACGTCAATTGCAACGCGCGCTTCGAAGGCGTCTATACGAGCAACGAG
CTGCCGCATTTCATCAAGGACATGCGATATGCGTATCCCGGCAATCCGGCGCTGGGCCGATTGATTGCAGAGACGGCAAC
GGCGCGCGGCGTGTTCACCCGTGCGCACGAGATCAACAGCCTCGAACTCGAATACGGCACGCTTGTGCCGATGCGGTACA
TGAACAGCGAACAGCACTTCAAGGTCGTGTCGATTGCCGGGTGGTGTGCGTGGCACACACTGGAAGAAAGCCACCGCTTC
GGCGCAGCGCTGCTCGAGGCCATCGAGGCCAGCGACAGCACCGTCGCATTTCTCGCCAGCGGATCGCTCTCGCACCGTTT
CAACGACAACGGCAGCCCCGAAGAATCCATTCACCAGATCAGCCGCGAGTTCTTCAGGCAGGTCGATTTGCGCGTGGTCG
AGCTATGGAAGCAGGGTGACTGGAAGACCTTCTGCGCGATGCTGCCCGAGTACGCATCGCTGTGTGAAGGCGAGGGCGGC
ATGCACGACACCGCCATGCTGCTCGGCCTGCTTGGTTGGGATGCCTACGACAAAGGGGTCGAGATCGTCACCGAATATTT
CGCCAGTTCCGGCACGGGCCAGATCAACGCGATCTTTCCGTTGCCCGGACGTGATCAAGTTCAAGCCTGA

Upstream 100 bases:

>100_bases
AAATCAAGAACGTCTGCATCTCAATGGGCAGTCACCCTATCCCGCGCTGGGGCGTTTGACGCGCGGCCGACACACCACAA
CAAGGGCGGAAGGAGACACC

Downstream 100 bases:

>100_bases
GGAGATCGACATGCCGCATGTCATCGTCGAATACACCGACAACCTGGGCGATGACGCCCGCATCCCGCAATTGCTGCAGG
CGATGAACGAGACGCTGATG

Product: 3,4-dihydroxyphenylacetate 2,3-dioxygenase

Products: NA

Alternate protein names: Homoprotocatechuate 2,3-dioxygenase; HPC dioxygenase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MGKLALAAKITHVPSMYLSELPGKHHGCREAAIEGHRIIGQRCRDMGVDTIVVSDVHWLVNAGYHVNCNARFEGVYTSNE
LPHFIKDMRYAYPGNPALGRLIAETATARGVFTRAHEINSLELEYGTLVPMRYMNSEQHFKVVSIAGWCAWHTLEESHRF
GAALLEAIEASDSTVAFLASGSLSHRFNDNGSPEESIHQISREFFRQVDLRVVELWKQGDWKTFCAMLPEYASLCEGEGG
MHDTAMLLGLLGWDAYDKGVEIVTEYFASSGTGQINAIFPLPGRDQVQA

Sequences:

>Translated_289_residues
MGKLALAAKITHVPSMYLSELPGKHHGCREAAIEGHRIIGQRCRDMGVDTIVVSDVHWLVNAGYHVNCNARFEGVYTSNE
LPHFIKDMRYAYPGNPALGRLIAETATARGVFTRAHEINSLELEYGTLVPMRYMNSEQHFKVVSIAGWCAWHTLEESHRF
GAALLEAIEASDSTVAFLASGSLSHRFNDNGSPEESIHQISREFFRQVDLRVVELWKQGDWKTFCAMLPEYASLCEGEGG
MHDTAMLLGLLGWDAYDKGVEIVTEYFASSGTGQINAIFPLPGRDQVQA
>Mature_288_residues
GKLALAAKITHVPSMYLSELPGKHHGCREAAIEGHRIIGQRCRDMGVDTIVVSDVHWLVNAGYHVNCNARFEGVYTSNEL
PHFIKDMRYAYPGNPALGRLIAETATARGVFTRAHEINSLELEYGTLVPMRYMNSEQHFKVVSIAGWCAWHTLEESHRFG
AALLEAIEASDSTVAFLASGSLSHRFNDNGSPEESIHQISREFFRQVDLRVVELWKQGDWKTFCAMLPEYASLCEGEGGM
HDTAMLLGLLGWDAYDKGVEIVTEYFASSGTGQINAIFPLPGRDQVQA

Specific function: Transforms homoprotocatechuic acid (HPC) into 5- carboxymethyl-2-hydroxy-muconic semialdehyde (CHMS) [H]

COG id: COG3384

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011984
- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.15 [H]

Molecular weight: Translated: 32118; Mature: 31987

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLALAAKITHVPSMYLSELPGKHHGCREAAIEGHRIIGQRCRDMGVDTIVVSDVHWLV
CCCHHHHHHHHHCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHH
NAGYHVNCNARFEGVYTSNELPHFIKDMRYAYPGNPALGRLIAETATARGVFTRAHEINS
CCCEEEECCEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
LELEYGTLVPMRYMNSEQHFKVVSIAGWCAWHTLEESHRFGAALLEAIEASDSTVAFLAS
EEEECCCCCHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
GSLSHRFNDNGSPEESIHQISREFFRQVDLRVVELWKQGDWKTFCAMLPEYASLCEGEGG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
MHDTAMLLGLLGWDAYDKGVEIVTEYFASSGTGQINAIFPLPGRDQVQA
CHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure 
GKLALAAKITHVPSMYLSELPGKHHGCREAAIEGHRIIGQRCRDMGVDTIVVSDVHWLV
CCHHHHHHHHHCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHH
NAGYHVNCNARFEGVYTSNELPHFIKDMRYAYPGNPALGRLIAETATARGVFTRAHEINS
CCCEEEECCEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
LELEYGTLVPMRYMNSEQHFKVVSIAGWCAWHTLEESHRFGAALLEAIEASDSTVAFLAS
EEEECCCCCHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
GSLSHRFNDNGSPEESIHQISREFFRQVDLRVVELWKQGDWKTFCAMLPEYASLCEGEGG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
MHDTAMLLGLLGWDAYDKGVEIVTEYFASSGTGQINAIFPLPGRDQVQA
CHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2261999 [H]