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The map label for this gene is pdhB [H]

Identifier: 187929026

GI number: 187929026

Start: 2052360

End: 2054033

Strand: Reverse

Name: pdhB [H]

Synonym: Rpic_1946

Alternate gene names: 187929026

Gene position: 2054033-2052360 (Counterclockwise)

Preceding gene: 187929027

Following gene: 187929025

Centisome position: 52.1

GC content: 65.59

Gene sequence:

>1674_bases
ATGAGTCAAGTCGTAGAAATCAAGGTGCCGGACATCGGCGACTACAAAGACGTCCCGGTGATCGAAGTGCTGGTCAAGGC
GGGCGATACCGTCAACGCCGAAGATTCGCTGGTTACGCTGGAATCGGACAAGGCGACCATGGACGTGCCCTCGCCCAAGA
GCGGCGTCGTCAAGGAAGTGAAGATCAAGGTGGGTGACACCGTGTCGGAAGGCTCGCTGGTGCTGTTGCTCGAAGAGCAG
GGCGCTGCTGCCGCACCGGCCCCGGCACCTCAAGCTGCGCCCGCACCGGCTCCGGCCGCTGCTGCACCGGCGCCCGCACC
CGCCGCGCAAGCTCCGGCCCCTGCGCCGGCTGCCGCTGGCGGCGGCACGGTTGAAGTGAAGGTGCCGGACATCGGCGACT
ACACCGACGTGCCCGTCATCGAAATCAGCGTGAAGGTGGGCGACAGGGTGGAAGCCGAGCAGTCGCTGATCACGCTGGAA
TCGGACAAGGCCACGATGGACGTGCCGTCGCCGGCTGCCGGCACGGTCAAGGAAATCCGCGTGAAGGTGGGCGATGCCGT
GTCGCAAGGCACGCTGATCGTCGTCCTGGAAGGTGCTGGCGGCGCTGCTGCTGCGCCAGCACCCGCACAAGCGCCGGTGT
CCGCACCGGCTGCCGCTGCGCCGAGCCCGGCCCCTGCGGCTGCGCCCGCAGTTGCGTCGACCGCAGCGCCCGCTACCTAC
ACCGCTGACACGGTCGGTACGGTTGGCAAGGCTGCTCACGCCAGCCCCTCGGTGCGCAAGTACGCGCGCGAGCTGGGCGT
CAACGTGAATCTGGTCGGGGGCACGGGTCCGAAGAACCGCATCACGCAGGAAGACGTGCAGCGCTACGTCAAGGGCGTGA
TGAGCGGCCAGGCTGCGGCGCCGGGCAAGGCTGCCGCGGGTGCACCGGCCGGTGGCGGTGAGTTGAATCTGCTGCCGTGG
CCGAAGGTGGACTTCACCAAGTTCGGTCCGGTCGATCCGAAGCCGCTGTCGCGTATCAAGAAGATTTCCGGCGCGAACCT
GCACCGCAACTGGGTCATGATTCCGCACGTCACCAACAACGACGAGGCGGACATCACCGAACTGGAAGCCTTCCGCGTGC
AGATGAACAAGGACCACGAAAAGGCCGGCGTGAAGTTCACGATGCTGGCATTCGTGATCAAGGCAGTCGTGGGCGCGCTG
AAGAAATTCCCGACCTTCAACGCGAGCCTGGACGGCGACAACCTGGTCTTCAAGCAGTACTTCCACGTCGGCTTCGCGGC
CGATACGCCCAACGGGCTCGTGGTGCCGGTGATCCGCGATGCGGACAAGAAGGGTCTGATCGACATCGCCAAGGAGATGG
CGGACCTGTCGAAGGCTGCCCGCGAAGGCAAGCTCAAGCCGGACCAGATGCAGGGGGGCTGCTTCTCGATCTCGTCACTG
GGCGGCATCGGCGGCACGCATTTCACGCCGATCATCAACGCACCGGAAGTGGCCATCCTTGGCTTGTCGCGTGGTTATCA
GAAGCCCGTTTGGGACGGCAAGCAGTTCGTGCCGCGCCTGACGCTGCCGCTGTCGCTGTCGTACGATCACCGCGTGATCG
ACGGCGCCGAGGCTGCACGCTTCAACGCGTATCTGGCCAGCGTGCTGGCGGACTTCCGCCGCGTCAGCCTGTAA

Upstream 100 bases:

>100_bases
CTTTCCGCAACGTGCATTTGCCTGCACGATGCGGTAGCCTGCGGGCACCGCGTGTCCGCCGCCGGATGCTCTCGCCGCCA
GACCCTCTGGAGACAGAAGA

Downstream 100 bases:

>100_bases
GCCCACGCGGGCGCGGCACGCATCTGGCTTCGGGCCTGTGCGTGCCGGCCCGGTTGGGTTTGCAGCGGTCGGCGGACTGG
TCAGTGGATTGATGGCGAGG

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MSQVVEIKVPDIGDYKDVPVIEVLVKAGDTVNAEDSLVTLESDKATMDVPSPKSGVVKEVKIKVGDTVSEGSLVLLLEEQ
GAAAAPAPAPQAAPAPAPAAAAPAPAPAAQAPAPAPAAAGGGTVEVKVPDIGDYTDVPVIEISVKVGDRVEAEQSLITLE
SDKATMDVPSPAAGTVKEIRVKVGDAVSQGTLIVVLEGAGGAAAAPAPAQAPVSAPAAAAPSPAPAAAPAVASTAAPATY
TADTVGTVGKAAHASPSVRKYARELGVNVNLVGGTGPKNRITQEDVQRYVKGVMSGQAAAPGKAAAGAPAGGGELNLLPW
PKVDFTKFGPVDPKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEAFRVQMNKDHEKAGVKFTMLAFVIKAVVGAL
KKFPTFNASLDGDNLVFKQYFHVGFAADTPNGLVVPVIRDADKKGLIDIAKEMADLSKAAREGKLKPDQMQGGCFSISSL
GGIGGTHFTPIINAPEVAILGLSRGYQKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLASVLADFRRVSL

Sequences:

>Translated_557_residues
MSQVVEIKVPDIGDYKDVPVIEVLVKAGDTVNAEDSLVTLESDKATMDVPSPKSGVVKEVKIKVGDTVSEGSLVLLLEEQ
GAAAAPAPAPQAAPAPAPAAAAPAPAPAAQAPAPAPAAAGGGTVEVKVPDIGDYTDVPVIEISVKVGDRVEAEQSLITLE
SDKATMDVPSPAAGTVKEIRVKVGDAVSQGTLIVVLEGAGGAAAAPAPAQAPVSAPAAAAPSPAPAAAPAVASTAAPATY
TADTVGTVGKAAHASPSVRKYARELGVNVNLVGGTGPKNRITQEDVQRYVKGVMSGQAAAPGKAAAGAPAGGGELNLLPW
PKVDFTKFGPVDPKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEAFRVQMNKDHEKAGVKFTMLAFVIKAVVGAL
KKFPTFNASLDGDNLVFKQYFHVGFAADTPNGLVVPVIRDADKKGLIDIAKEMADLSKAAREGKLKPDQMQGGCFSISSL
GGIGGTHFTPIINAPEVAILGLSRGYQKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLASVLADFRRVSL
>Mature_556_residues
SQVVEIKVPDIGDYKDVPVIEVLVKAGDTVNAEDSLVTLESDKATMDVPSPKSGVVKEVKIKVGDTVSEGSLVLLLEEQG
AAAAPAPAPQAAPAPAPAAAAPAPAPAAQAPAPAPAAAGGGTVEVKVPDIGDYTDVPVIEISVKVGDRVEAEQSLITLES
DKATMDVPSPAAGTVKEIRVKVGDAVSQGTLIVVLEGAGGAAAAPAPAQAPVSAPAAAAPSPAPAAAPAVASTAAPATYT
ADTVGTVGKAAHASPSVRKYARELGVNVNLVGGTGPKNRITQEDVQRYVKGVMSGQAAAPGKAAAGAPAGGGELNLLPWP
KVDFTKFGPVDPKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEAFRVQMNKDHEKAGVKFTMLAFVIKAVVGALK
KFPTFNASLDGDNLVFKQYFHVGFAADTPNGLVVPVIRDADKKGLIDIAKEMADLSKAAREGKLKPDQMQGGCFSISSLG
GIGGTHFTPIINAPEVAILGLSRGYQKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLASVLADFRRVSL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=447, Percent_Identity=29.9776286353468, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI31711992, Length=433, Percent_Identity=31.4087759815243, Blast_Score=139, Evalue=1e-32,
Organism=Homo sapiens, GI203098816, Length=475, Percent_Identity=27.5789473684211, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI19923748, Length=222, Percent_Identity=32.4324324324324, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI203098753, Length=438, Percent_Identity=27.6255707762557, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI260898739, Length=153, Percent_Identity=35.9477124183007, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1786305, Length=556, Percent_Identity=51.0791366906475, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI1786946, Length=437, Percent_Identity=30.4347826086957, Blast_Score=175, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17537937, Length=427, Percent_Identity=28.5714285714286, Blast_Score=161, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17560088, Length=437, Percent_Identity=30.4347826086957, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI25146366, Length=200, Percent_Identity=38.5, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17538894, Length=307, Percent_Identity=30.2931596091205, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320352, Length=441, Percent_Identity=29.0249433106576, Blast_Score=170, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6324258, Length=431, Percent_Identity=28.3062645011601, Blast_Score=123, Evalue=7e-29,
Organism=Drosophila melanogaster, GI18859875, Length=438, Percent_Identity=31.0502283105023, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24645909, Length=207, Percent_Identity=34.7826086956522, Blast_Score=125, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24582497, Length=222, Percent_Identity=32.8828828828829, Blast_Score=125, Evalue=1e-28,
Organism=Drosophila melanogaster, GI20129315, Length=222, Percent_Identity=32.8828828828829, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 57312; Mature: 57181

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVVEIKVPDIGDYKDVPVIEVLVKAGDTVNAEDSLVTLESDKATMDVPSPKSGVVKEV
CCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCEECCCCCCCCCEEEE
KIKVGDTVSEGSLVLLLEEQGAAAAPAPAPQAAPAPAPAAAAPAPAPAAQAPAPAPAAAG
EEEECCEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGTVEVKVPDIGDYTDVPVIEISVKVGDRVEAEQSLITLESDKATMDVPSPAAGTVKEIR
CCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCEECCCCCCCCCHHHHH
VKVGDAVSQGTLIVVLEGAGGAAAAPAPAQAPVSAPAAAAPSPAPAAAPAVASTAAPATY
HHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEE
TADTVGTVGKAAHASPSVRKYARELGVNVNLVGGTGPKNRITQEDVQRYVKGVMSGQAAA
ECCCHHHCCCHHCCCHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCC
PGKAAAGAPAGGGELNLLPWPKVDFTKFGPVDPKPLSRIKKISGANLHRNWVMIPHVTNN
CCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCEECCEEEEEEECCC
DEADITELEAFRVQMNKDHEKAGVKFTMLAFVIKAVVGALKKFPTFNASLDGDNLVFKQY
CCCCHHHHHHHHEECCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEH
FHVGFAADTPNGLVVPVIRDADKKGLIDIAKEMADLSKAAREGKLKPDQMQGGCFSISSL
HHEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCC
GGIGGTHFTPIINAPEVAILGLSRGYQKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAAR
CCCCCCCCCEECCCCCEEEEECCCCCCCCCCCCHHHCCEEEEEEEECCCCEEECCCHHHH
FNAYLASVLADFRRVSL
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQVVEIKVPDIGDYKDVPVIEVLVKAGDTVNAEDSLVTLESDKATMDVPSPKSGVVKEV
CCEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCEECCCCCCCCCEEEE
KIKVGDTVSEGSLVLLLEEQGAAAAPAPAPQAAPAPAPAAAAPAPAPAAQAPAPAPAAAG
EEEECCEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGTVEVKVPDIGDYTDVPVIEISVKVGDRVEAEQSLITLESDKATMDVPSPAAGTVKEIR
CCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCEECCCCCCCCCHHHHH
VKVGDAVSQGTLIVVLEGAGGAAAAPAPAQAPVSAPAAAAPSPAPAAAPAVASTAAPATY
HHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEE
TADTVGTVGKAAHASPSVRKYARELGVNVNLVGGTGPKNRITQEDVQRYVKGVMSGQAAA
ECCCHHHCCCHHCCCHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCC
PGKAAAGAPAGGGELNLLPWPKVDFTKFGPVDPKPLSRIKKISGANLHRNWVMIPHVTNN
CCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCEECCEEEEEEECCC
DEADITELEAFRVQMNKDHEKAGVKFTMLAFVIKAVVGALKKFPTFNASLDGDNLVFKQY
CCCCHHHHHHHHEECCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEH
FHVGFAADTPNGLVVPVIRDADKKGLIDIAKEMADLSKAAREGKLKPDQMQGGCFSISSL
HHEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCC
GGIGGTHFTPIINAPEVAILGLSRGYQKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAAR
CCCCCCCCCEECCCCCEEEEECCCCCCCCCCCCHHHCCEEEEEEEECCCCEEECCCHHHH
FNAYLASVLADFRRVSL
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]