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The map label for this gene is livJ [C]

Identifier: 187928540

GI number: 187928540

Start: 1544966

End: 1546222

Strand: Reverse

Name: livJ [C]

Synonym: Rpic_1454

Alternate gene names: 187928540

Gene position: 1546222-1544966 (Counterclockwise)

Preceding gene: 187928541

Following gene: 187928539

Centisome position: 39.22

GC content: 59.67

Gene sequence:

>1257_bases
ATGACGAAGTTCCGTCCGTTGGTTGTGGCTGTTGCATGTGTTGCGGCAATCGGTGGGGCAGCTCTGCCCACTGCAGCGTC
CGCCGCCGAAACCGTGAAGATCGCCTGGATCGATCCGTTGTCCGGCCTGATGGGCGCGCTGGGTCAGAACCAGTTGCGCA
GCTGGCAGTACATTGCCGATCTCGCCACGCAGAAGAACTGGGCCGGCGACGGCACGAAGTTCGAAGTGGTGGGCTTCGAC
AACAAGCTCTCGCCGCAGGAAAGCCTGACGGCGCTCAAGCAGGTGGCTGATCAGGGCATCCACTACATCGTGCAGGGCAA
CGGCTCGAGCGTGGGCATGGCGCTGGAAGACGCCGTGGCCAAGTACAACGAGCGTAATCCGGGTAAGGAAATCGTCTACC
TGAACTACGCTGCGGTGGATCCGGACATGACGAACAGCAAGTGCAATTACTGGCACTTCCGCCTGGACGCCAACTCCGAC
ATGAAGATGGAAGCGCTGACCAGCTTCCTGGCGAAAGATCCGAAGGTCAAGAAGGTCTACCTGATCAACCAGAACTATTC
GTTCGGCCATCAGGTCGCGCGTGCGGCCAAGGAATACCTCAAGCGCAAGCGTCCAGACATCGAGATCGTGGGTGAAGACC
TGCACCCGCTGGCGCAGGTGAAGGACTTCTCGCCGTACGTCTCGAAGATCAAGTCGTCGGGTGCCGACACCGTCATCACC
GGCAACTGGGGCAGCGACCTGGCACTGCTCATCAAAGCCGGCAAGGATGCAGGCCTGAACGCGAACTTCTATACGTACTA
CGCCTCGACCACGGGCGTTCCGACAGCGATGGGCTCCGCAGGGGCAGATCATGTGAAATACGTGGGCTACTGGAACGTCA
ACAACGACGGCTACAAGGGCGCCGATATCGTCGAGGGCTACAAGAAGAAGTACAACGACGATTACTACCTCATGGCGTCG
TACACCGGCATCGCCATGCTGGCCAAGGCCATCAAGCAGACGAAGTCGGCCGAGCCTGCCAAGGTGGCCAAGGCCTTCGA
AGGCATGAAGGTCGACAGCCTGAACGGCACTTTCGAGATGCGCGCGTCTGACCACCAGGGCCAGCAGCCGCTCTACATCG
CCACCTGGGAAAAAACCAACGGCAAGAACGTCAAGTTCGACCAGGAAAACACCGGCTACGGCTGGAAGACCGATGCGGTG
CTCGACCAGTACGTCGCGTCGCAGCCGACTTCCTGCCAGATGAAGCGTCCACAGTAA

Upstream 100 bases:

>100_bases
CCCGGGCGCGTTGCAACCTGGAGACGCAAGCAGCCGCACGCCCACCAGAGGCATGCGGAGAGTGACTCGCCGGCGGCTCC
ACCACATGAAGGAGACACGC

Downstream 100 bases:

>100_bases
GGCGGCGGGTGGTGTGCCGAAGGGCCGTCCGATGCTATCGTGCGGCGATGTTTTAAACGGAGTCCATGGGCCAGCGCATT
GATGCGCTCGCCCGTGCTCC

Product: putative Leu/Ile/Val-binding signal peptide protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MTKFRPLVVAVACVAAIGGAALPTAASAAETVKIAWIDPLSGLMGALGQNQLRSWQYIADLATQKNWAGDGTKFEVVGFD
NKLSPQESLTALKQVADQGIHYIVQGNGSSVGMALEDAVAKYNERNPGKEIVYLNYAAVDPDMTNSKCNYWHFRLDANSD
MKMEALTSFLAKDPKVKKVYLINQNYSFGHQVARAAKEYLKRKRPDIEIVGEDLHPLAQVKDFSPYVSKIKSSGADTVIT
GNWGSDLALLIKAGKDAGLNANFYTYYASTTGVPTAMGSAGADHVKYVGYWNVNNDGYKGADIVEGYKKKYNDDYYLMAS
YTGIAMLAKAIKQTKSAEPAKVAKAFEGMKVDSLNGTFEMRASDHQGQQPLYIATWEKTNGKNVKFDQENTGYGWKTDAV
LDQYVASQPTSCQMKRPQ

Sequences:

>Translated_418_residues
MTKFRPLVVAVACVAAIGGAALPTAASAAETVKIAWIDPLSGLMGALGQNQLRSWQYIADLATQKNWAGDGTKFEVVGFD
NKLSPQESLTALKQVADQGIHYIVQGNGSSVGMALEDAVAKYNERNPGKEIVYLNYAAVDPDMTNSKCNYWHFRLDANSD
MKMEALTSFLAKDPKVKKVYLINQNYSFGHQVARAAKEYLKRKRPDIEIVGEDLHPLAQVKDFSPYVSKIKSSGADTVIT
GNWGSDLALLIKAGKDAGLNANFYTYYASTTGVPTAMGSAGADHVKYVGYWNVNNDGYKGADIVEGYKKKYNDDYYLMAS
YTGIAMLAKAIKQTKSAEPAKVAKAFEGMKVDSLNGTFEMRASDHQGQQPLYIATWEKTNGKNVKFDQENTGYGWKTDAV
LDQYVASQPTSCQMKRPQ
>Mature_417_residues
TKFRPLVVAVACVAAIGGAALPTAASAAETVKIAWIDPLSGLMGALGQNQLRSWQYIADLATQKNWAGDGTKFEVVGFDN
KLSPQESLTALKQVADQGIHYIVQGNGSSVGMALEDAVAKYNERNPGKEIVYLNYAAVDPDMTNSKCNYWHFRLDANSDM
KMEALTSFLAKDPKVKKVYLINQNYSFGHQVARAAKEYLKRKRPDIEIVGEDLHPLAQVKDFSPYVSKIKSSGADTVITG
NWGSDLALLIKAGKDAGLNANFYTYYASTTGVPTAMGSAGADHVKYVGYWNVNNDGYKGADIVEGYKKKYNDDYYLMASY
TGIAMLAKAIKQTKSAEPAKVAKAFEGMKVDSLNGTFEMRASDHQGQQPLYIATWEKTNGKNVKFDQENTGYGWKTDAVL
DQYVASQPTSCQMKRPQ

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=348, Percent_Identity=21.8390804597701, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000709 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 45788; Mature: 45657

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS00995 TCP1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFRPLVVAVACVAAIGGAALPTAASAAETVKIAWIDPLSGLMGALGQNQLRSWQYIAD
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCHHHHHHHCCHHHHHHHHHHHH
LATQKNWAGDGTKFEVVGFDNKLSPQESLTALKQVADQGIHYIVQGNGSSVGMALEDAVA
HHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHH
KYNERNPGKEIVYLNYAAVDPDMTNSKCNYWHFRLDANSDMKMEALTSFLAKDPKVKKVY
HHCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEE
LINQNYSFGHQVARAAKEYLKRKRPDIEIVGEDLHPLAQVKDFSPYVSKIKSSGADTVIT
EEECCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCHHHHHHHCCCCCEEEE
GNWGSDLALLIKAGKDAGLNANFYTYYASTTGVPTAMGSAGADHVKYVGYWNVNNDGYKG
CCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCC
ADIVEGYKKKYNDDYYLMASYTGIAMLAKAIKQTKSAEPAKVAKAFEGMKVDSLNGTFEM
HHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCEEEE
RASDHQGQQPLYIATWEKTNGKNVKFDQENTGYGWKTDAVLDQYVASQPTSCQMKRPQ
ECCCCCCCCCEEEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TKFRPLVVAVACVAAIGGAALPTAASAAETVKIAWIDPLSGLMGALGQNQLRSWQYIAD
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCHHHHHHHCCHHHHHHHHHHHH
LATQKNWAGDGTKFEVVGFDNKLSPQESLTALKQVADQGIHYIVQGNGSSVGMALEDAVA
HHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHH
KYNERNPGKEIVYLNYAAVDPDMTNSKCNYWHFRLDANSDMKMEALTSFLAKDPKVKKVY
HHCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEE
LINQNYSFGHQVARAAKEYLKRKRPDIEIVGEDLHPLAQVKDFSPYVSKIKSSGADTVIT
EEECCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCHHHHHHHCCCCCEEEE
GNWGSDLALLIKAGKDAGLNANFYTYYASTTGVPTAMGSAGADHVKYVGYWNVNNDGYKG
CCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCC
ADIVEGYKKKYNDDYYLMASYTGIAMLAKAIKQTKSAEPAKVAKAFEGMKVDSLNGTFEM
HHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCEEEE
RASDHQGQQPLYIATWEKTNGKNVKFDQENTGYGWKTDAVLDQYVASQPTSCQMKRPQ
ECCCCCCCCCEEEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA