Click here to switch to the map view.

The map label for this gene is pncA [H]

Identifier: 187928532

GI number: 187928532

Start: 1534710

End: 1535351

Strand: Reverse

Name: pncA [H]

Synonym: Rpic_1446

Alternate gene names: 187928532

Gene position: 1535351-1534710 (Counterclockwise)

Preceding gene: 187928533

Following gene: 187928531

Centisome position: 38.94

GC content: 64.17

Gene sequence:

>642_bases
ATGCAACTGACGTCCACCGATTGCCTGTTGATCATCGACGTGCAGAACGATTTTCTGCCCGGCGGTGCACTGGCCGTGCC
GGACGGCGACCAGGTCATCCCGGTCGTCAACCTGCTCGCCAAGGCATTCGGGGCCGTTGGTCGTACTGTGCTGACGCAGG
ACTGGCACCCGAGCGAGCACGTCTCGTTCGCGGCCAACCATCCGGGCACGCAGCCGTTTGGCACGATCGATTTGCCGTAC
GGGCAGCAAGTGCTGTGGCCGGTCCACTGCGTGCAGCACAGCAAAGGTGCCGCGCTGGCCGATGGGCTGAATGTGCCGCA
TGCCCAGTTGATCGTCCGCAAGGGCTATCACCCGCACATCGACAGCTATTCCGCATTCTTTGAAGCGGATCGCAAGACGC
CGACCGGCCTGCTCGGCTACCTGCGCGAGCTTGGGGTCGAGCGCGTGTTCTGCGTTGGCCTCGCCACGGATTTTTGCGTG
GCGTGGTCGGCGCTGGATGCGCGTGCGGCGGGGCTGGAAGTGGTCGTCATCGAGGATGCCTGTCGCGCGATCGACCTCAA
CGGTTCGCTGGCGCAGGCGTGGCAACGCATGACGGATGCGGGCATCGCGCGGTTGCAGTCGCACACAGTCTTGAACGGAT
AA

Upstream 100 bases:

>100_bases
GTGGAAGGTTGCCCCGCTGCTGCAGAAGCTGGCCGATGAAGGCAAGGGCTTTAATGGCTGAATGAGCAGCTAATCAATCG
ACTGACGCGACTGAACCACC

Downstream 100 bases:

>100_bases
CGGAACACATCGGGCGCTGCCGGAGCCGCGCCCGCCACCAGATACGAGGAGCAACACCATGACCGAAGCAGTCATCGTTT
CCACCGCACGTACCGGCCTG

Product: Nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MQLTSTDCLLIIDVQNDFLPGGALAVPDGDQVIPVVNLLAKAFGAVGRTVLTQDWHPSEHVSFAANHPGTQPFGTIDLPY
GQQVLWPVHCVQHSKGAALADGLNVPHAQLIVRKGYHPHIDSYSAFFEADRKTPTGLLGYLRELGVERVFCVGLATDFCV
AWSALDARAAGLEVVVIEDACRAIDLNGSLAQAWQRMTDAGIARLQSHTVLNG

Sequences:

>Translated_213_residues
MQLTSTDCLLIIDVQNDFLPGGALAVPDGDQVIPVVNLLAKAFGAVGRTVLTQDWHPSEHVSFAANHPGTQPFGTIDLPY
GQQVLWPVHCVQHSKGAALADGLNVPHAQLIVRKGYHPHIDSYSAFFEADRKTPTGLLGYLRELGVERVFCVGLATDFCV
AWSALDARAAGLEVVVIEDACRAIDLNGSLAQAWQRMTDAGIARLQSHTVLNG
>Mature_213_residues
MQLTSTDCLLIIDVQNDFLPGGALAVPDGDQVIPVVNLLAKAFGAVGRTVLTQDWHPSEHVSFAANHPGTQPFGTIDLPY
GQQVLWPVHCVQHSKGAALADGLNVPHAQLIVRKGYHPHIDSYSAFFEADRKTPTGLLGYLRELGVERVFCVGLATDFCV
AWSALDARAAGLEVVVIEDACRAIDLNGSLAQAWQRMTDAGIARLQSHTVLNG

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=195, Percent_Identity=42.5641025641026, Blast_Score=152, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI193206735, Length=231, Percent_Identity=28.5714285714286, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17543130, Length=230, Percent_Identity=28.695652173913, Blast_Score=91, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71999339, Length=198, Percent_Identity=31.3131313131313, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321401, Length=190, Percent_Identity=35.7894736842105, Blast_Score=120, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24648247, Length=201, Percent_Identity=35.8208955223881, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22888; Mature: 22888

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLTSTDCLLIIDVQNDFLPGGALAVPDGDQVIPVVNLLAKAFGAVGRTVLTQDWHPSEH
CCCCCCCEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCHHHEECCCCCCCC
VSFAANHPGTQPFGTIDLPYGQQVLWPVHCVQHSKGAALADGLNVPHAQLIVRKGYHPHI
EEEECCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCEECCCCCCCHHHHHEECCCCCCC
DSYSAFFEADRKTPTGLLGYLRELGVERVFCVGLATDFCVAWSALDARAAGLEVVVIEDA
CCHHHHHHCCCCCHHHHHHHHHHHCCHHEEEHHHHHHHHHHHHHHHHHHCCEEEEEEECC
CRAIDLNGSLAQAWQRMTDAGIARLQSHTVLNG
CCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQLTSTDCLLIIDVQNDFLPGGALAVPDGDQVIPVVNLLAKAFGAVGRTVLTQDWHPSEH
CCCCCCCEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCHHHEECCCCCCCC
VSFAANHPGTQPFGTIDLPYGQQVLWPVHCVQHSKGAALADGLNVPHAQLIVRKGYHPHI
EEEECCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCEECCCCCCCHHHHHEECCCCCCC
DSYSAFFEADRKTPTGLLGYLRELGVERVFCVGLATDFCVAWSALDARAAGLEVVVIEDA
CCHHHHHHCCCCCHHHHHHHHHHHCCHHEEEHHHHHHHHHHHHHHHHHHCCEEEEEEECC
CRAIDLNGSLAQAWQRMTDAGIARLQSHTVLNG
CCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]