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The map label for this gene is holB [H]

Identifier: 187928510

GI number: 187928510

Start: 1513409

End: 1514419

Strand: Reverse

Name: holB [H]

Synonym: Rpic_1424

Alternate gene names: 187928510

Gene position: 1514419-1513409 (Counterclockwise)

Preceding gene: 187928511

Following gene: 187928509

Centisome position: 38.41

GC content: 65.58

Gene sequence:

>1011_bases
ATGATGCTTTATCCTTGGCAATTCGCTGATTGGTCCCGCCTGCAAGCCATGCGGGCCAAGCTGCCGCACGCGATTCTGCT
CAACGGCCAGGCCGGCACCGGCAAGCGCGATTTCGCCATGCACTTCGCGCAAAGCCTGCTGTGCGATACGCCGGCGGCCG
ACGGCCAGCCCTGCGGCACATGCGCGGCCTGCAACTGGTTTGCGCAGGGCAACCACCCGGACTTCCACCTCGTGCGCCCT
GAAGCGATGGAAGACGCGCCCGAGACGGAGAAGGACGCCGACGGCAAGAAAAAGGCGCCGAGCAAGATCATCCGCATGGA
GCAGGTCCGTAACCTGATCGAAGGCGTGGGGGTCGGTACGCACCGGGCCGGGTTGCGGGTGGTGGTGCTGTATCCGCTCG
ACGCGTTGCAGACCGAAGGCGCCAACGCGTTGCTCAAGACGCTGGAAGAGCCGCCGCAGAACACCGTCTTCCTGCTGGTG
ACGGATCGTATCGACCGTATCCTGCCGACCATTCTGTCGCGCTGCAGGCAGTTTCCGCTGCAGCCGCCGCAGCCCGAGGC
CGCGCGCCAGTGGCTGGAGCGGCAAGGCGTGCCGCACGCGCAGGATCTGCTGGCCGAGTTCGGTAACGCGCCGCTGGCCG
CGCTGGCCGCCGCCGAGTCCGAAGACCGTCCGCTGCTGCAATTCCTGCTTGAACAGCTCGGCCAGGGCGCAAAGCTCGAT
GCACTGGCCACGGCCGACCATTTACAAAAATTGTCGGTCCCGGCCGTTCTCAGTACCATGCACCGATGGGTCTTTGACTT
GATGGCGGCACGCTTGGCGGGGCGTCCGCGCTACTTTCCGGCGCAGCGCGATGTGCTGCAACGTTGCGCGAATACCATTC
CGCTGGACCGCCTGGAACGTTTTGCGCGTGCCTTGCCGCAGCGGCGCCGCTCCGAGCAGCATCCGCTGGCCGCGCGGCTC
GTCATCGAAAGCCTTTTGCTGGATTACCGGCAGCTCTTTCCGTCTGCCTGA

Upstream 100 bases:

>100_bases
TCTGCTTGCAACGCTCTGATTTTGCAGGCAATTTCGGATACGGAAATAATGGTTCCACGGCAAGTTTTCGCATCAATAAT
CTGACGCAACCCATTGATTC

Downstream 100 bases:

>100_bases
ACCCGAACCCGCACCTGAACGTTTGCACTCTGTGGACACCCTGTGAGCACTGCACCTCTTCGTACGCCCGGCACTCCCGT
CACCCCTGGCACCCCCGCTG

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MMLYPWQFADWSRLQAMRAKLPHAILLNGQAGTGKRDFAMHFAQSLLCDTPAADGQPCGTCAACNWFAQGNHPDFHLVRP
EAMEDAPETEKDADGKKKAPSKIIRMEQVRNLIEGVGVGTHRAGLRVVVLYPLDALQTEGANALLKTLEEPPQNTVFLLV
TDRIDRILPTILSRCRQFPLQPPQPEAARQWLERQGVPHAQDLLAEFGNAPLAALAAAESEDRPLLQFLLEQLGQGAKLD
ALATADHLQKLSVPAVLSTMHRWVFDLMAARLAGRPRYFPAQRDVLQRCANTIPLDRLERFARALPQRRRSEQHPLAARL
VIESLLLDYRQLFPSA

Sequences:

>Translated_336_residues
MMLYPWQFADWSRLQAMRAKLPHAILLNGQAGTGKRDFAMHFAQSLLCDTPAADGQPCGTCAACNWFAQGNHPDFHLVRP
EAMEDAPETEKDADGKKKAPSKIIRMEQVRNLIEGVGVGTHRAGLRVVVLYPLDALQTEGANALLKTLEEPPQNTVFLLV
TDRIDRILPTILSRCRQFPLQPPQPEAARQWLERQGVPHAQDLLAEFGNAPLAALAAAESEDRPLLQFLLEQLGQGAKLD
ALATADHLQKLSVPAVLSTMHRWVFDLMAARLAGRPRYFPAQRDVLQRCANTIPLDRLERFARALPQRRRSEQHPLAARL
VIESLLLDYRQLFPSA
>Mature_336_residues
MMLYPWQFADWSRLQAMRAKLPHAILLNGQAGTGKRDFAMHFAQSLLCDTPAADGQPCGTCAACNWFAQGNHPDFHLVRP
EAMEDAPETEKDADGKKKAPSKIIRMEQVRNLIEGVGVGTHRAGLRVVVLYPLDALQTEGANALLKTLEEPPQNTVFLLV
TDRIDRILPTILSRCRQFPLQPPQPEAARQWLERQGVPHAQDLLAEFGNAPLAALAAAESEDRPLLQFLLEQLGQGAKLD
ALATADHLQKLSVPAVLSTMHRWVFDLMAARLAGRPRYFPAQRDVLQRCANTIPLDRLERFARALPQRRRSEQHPLAARL
VIESLLLDYRQLFPSA

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=175, Percent_Identity=33.7142857142857, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787341, Length=261, Percent_Identity=32.5670498084291, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37544; Mature: 37544

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLYPWQFADWSRLQAMRAKLPHAILLNGQAGTGKRDFAMHFAQSLLCDTPAADGQPCGT
CCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCH
CAACNWFAQGNHPDFHLVRPEAMEDAPETEKDADGKKKAPSKIIRMEQVRNLIEGVGVGT
HHHHHHCCCCCCCCEEEECCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
HRAGLRVVVLYPLDALQTEGANALLKTLEEPPQNTVFLLVTDRIDRILPTILSRCRQFPL
CCCCCEEEEEECCHHHHCCCHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCC
QPPQPEAARQWLERQGVPHAQDLLAEFGNAPLAALAAAESEDRPLLQFLLEQLGQGAKLD
CCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCH
ALATADHLQKLSVPAVLSTMHRWVFDLMAARLAGRPRYFPAQRDVLQRCANTIPLDRLER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHH
FARALPQRRRSEQHPLAARLVIESLLLDYRQLFPSA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMLYPWQFADWSRLQAMRAKLPHAILLNGQAGTGKRDFAMHFAQSLLCDTPAADGQPCGT
CCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCH
CAACNWFAQGNHPDFHLVRPEAMEDAPETEKDADGKKKAPSKIIRMEQVRNLIEGVGVGT
HHHHHHCCCCCCCCEEEECCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
HRAGLRVVVLYPLDALQTEGANALLKTLEEPPQNTVFLLVTDRIDRILPTILSRCRQFPL
CCCCCEEEEEECCHHHHCCCHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCC
QPPQPEAARQWLERQGVPHAQDLLAEFGNAPLAALAAAESEDRPLLQFLLEQLGQGAKLD
CCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCH
ALATADHLQKLSVPAVLSTMHRWVFDLMAARLAGRPRYFPAQRDVLQRCANTIPLDRLER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHH
FARALPQRRRSEQHPLAARLVIESLLLDYRQLFPSA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]