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The map label for this gene is nepI [H]

Identifier: 187928492

GI number: 187928492

Start: 1495534

End: 1496745

Strand: Reverse

Name: nepI [H]

Synonym: Rpic_1406

Alternate gene names: 187928492

Gene position: 1496745-1495534 (Counterclockwise)

Preceding gene: 187928494

Following gene: 187928491

Centisome position: 37.96

GC content: 67.49

Gene sequence:

>1212_bases
ATGAATCCCGGAATCTCCACTGCCGCGTCAATCGCGGCCCCGCGTGAACCGGCCTGGGGTGCTGTCTTTGCAATGGCACT
GGGCGTGTTCGGGCTGGTCACGGCCGAATTCCTGCCTGCAAGCCTGCTGACGCCCATGGCGGAAAGCCTGGGCGTGACCG
AAGGCGTGGCAGGGCAGGCCGTCACGGCCACGGCAACGGTTGCGCTGGTCACCAGCTTGTTGACCGCCGCTGTCACGCGG
TCCATCGACCGGCGGCGCGTGCTGCTCGCATTCTCGGTGCTGCTTGTGGCATCCAATCTTGCGGTGGCGTTTGCAACCAG
CCTCACGACGATCCTGATCGGCCGCGTTGTGCTTGGCATTGCACTGGGCGGCTTCTGGACCATGGCGACCGCCACCGCAA
TGCGGCTCGTGCCACCGGCGTTGGTGCCGCGGGCGCTATCGATCATCTTCAGCGGCGTGGCCGTCGCCACGATTGCCGCC
GCGCCGCTGGGCAGCTACTTCGGCCACCTGATCGGCTGGCGCAATGTGTTCCTGGCCTCAGCGGGACTGGGAGTGATGGC
GTTTGTGTCGCAGGTCTCGACGCTGCCGTCGATGGCGCCAAGTGGCACAACGCGCCTGCGCACGCTGATCGACGTGCTGC
GCCGTCCGAGCGTGGGCATCGGGATGTTCGCGACGATCCTGGTGTTCACCGGGCACTTTGCGTTCTTCACGTACCTGCGG
CCGTTCCTGGAGCAGGTGGCGGGCGTTGGCGTGAACGGGCTGTCGGCCATCCTGTTGGGCTACGGCATTGCCAACTTCGT
CGGCACATCGTTGGCCGGCCGCGTGCTGGAACACCGGCTGCGCCCAATGCTGATCGCAATGCCCGCGTTGATGGTCGTGC
TGGGCATCGCGCTCGTGGCGCTGGGCCGCGCACCGGTCATCGACGCTGTGCTGATCGCGCTGTGGGGCATGGCGTTTGGC
GGCGTGCCGGTGGCGTGGTCGACATGGGTGACGCGCACCGTGCCCGATGAAGCCGAAAGCGCGGGTGGCCTGATTGTTGC
AGCCGTACAGCTCGCGATCGCCACCGGCGCGGCAGCCGGTGGCTTGGTGTTCGACGCGAACGGCGCAGCTGGTGTGTTCA
TTGGCGCCGCCGTGGTGCTGGCCACTGCCGTCGCGACGATCGTGACGGGCGTGCCGAAGCGCGTCGAGGTGGTGCCGGCG
ATGGCCGAGTGA

Upstream 100 bases:

>100_bases
GATCAACAAGGTCGGAGTGTATGCCATAGACCGTCCGGCTATCGGGCCCTACCATGCGCCCTTATTGGTATTTTCCCTGA
TGTTTTCGACGGAACGACGC

Downstream 100 bases:

>100_bases
ATTGGCGAGAATGGCGAGATGCGCGCTGCGAGGCTGGCCGGACGATGTCCCGCACCACTCAGCGCATGCGTCTATATTTC
TCATAATTTGCATTATGAAA

Product: major facilitator superfamily protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MNPGISTAASIAAPREPAWGAVFAMALGVFGLVTAEFLPASLLTPMAESLGVTEGVAGQAVTATATVALVTSLLTAAVTR
SIDRRRVLLAFSVLLVASNLAVAFATSLTTILIGRVVLGIALGGFWTMATATAMRLVPPALVPRALSIIFSGVAVATIAA
APLGSYFGHLIGWRNVFLASAGLGVMAFVSQVSTLPSMAPSGTTRLRTLIDVLRRPSVGIGMFATILVFTGHFAFFTYLR
PFLEQVAGVGVNGLSAILLGYGIANFVGTSLAGRVLEHRLRPMLIAMPALMVVLGIALVALGRAPVIDAVLIALWGMAFG
GVPVAWSTWVTRTVPDEAESAGGLIVAAVQLAIATGAAAGGLVFDANGAAGVFIGAAVVLATAVATIVTGVPKRVEVVPA
MAE

Sequences:

>Translated_403_residues
MNPGISTAASIAAPREPAWGAVFAMALGVFGLVTAEFLPASLLTPMAESLGVTEGVAGQAVTATATVALVTSLLTAAVTR
SIDRRRVLLAFSVLLVASNLAVAFATSLTTILIGRVVLGIALGGFWTMATATAMRLVPPALVPRALSIIFSGVAVATIAA
APLGSYFGHLIGWRNVFLASAGLGVMAFVSQVSTLPSMAPSGTTRLRTLIDVLRRPSVGIGMFATILVFTGHFAFFTYLR
PFLEQVAGVGVNGLSAILLGYGIANFVGTSLAGRVLEHRLRPMLIAMPALMVVLGIALVALGRAPVIDAVLIALWGMAFG
GVPVAWSTWVTRTVPDEAESAGGLIVAAVQLAIATGAAAGGLVFDANGAAGVFIGAAVVLATAVATIVTGVPKRVEVVPA
MAE
>Mature_403_residues
MNPGISTAASIAAPREPAWGAVFAMALGVFGLVTAEFLPASLLTPMAESLGVTEGVAGQAVTATATVALVTSLLTAAVTR
SIDRRRVLLAFSVLLVASNLAVAFATSLTTILIGRVVLGIALGGFWTMATATAMRLVPPALVPRALSIIFSGVAVATIAA
APLGSYFGHLIGWRNVFLASAGLGVMAFVSQVSTLPSMAPSGTTRLRTLIDVLRRPSVGIGMFATILVFTGHFAFFTYLR
PFLEQVAGVGVNGLSAILLGYGIANFVGTSLAGRVLEHRLRPMLIAMPALMVVLGIALVALGRAPVIDAVLIALWGMAFG
GVPVAWSTWVTRTVPDEAESAGGLIVAAVQLAIATGAAAGGLVFDANGAAGVFIGAAVVLATAVATIVTGVPKRVEVVPA
MAE

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and especially inosine. Involved in the resistance to 6-mercaptopurine [H]

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. NepI (TC 2.A.1.2.26) subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693198, Length=353, Percent_Identity=47.3087818696884, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI1787808, Length=239, Percent_Identity=30.5439330543933, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787947, Length=307, Percent_Identity=29.3159609120521, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1786595, Length=265, Percent_Identity=27.5471698113208, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 40911; Mature: 40911

Theoretical pI: Translated: 11.37; Mature: 11.37

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCC
>Mature Secondary Structure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CEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA