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The map label for this gene is leuC [H]

Identifier: 187928468

GI number: 187928468

Start: 1477901

End: 1479343

Strand: Direct

Name: leuC [H]

Synonym: Rpic_1381

Alternate gene names: 187928468

Gene position: 1477901-1479343 (Clockwise)

Preceding gene: 187928467

Following gene: 187928469

Centisome position: 37.49

GC content: 65.07

Gene sequence:

>1443_bases
ATGACACCTCAGACCTTGTTCGCAAAGCTGTGGCAACGCCACGCCGTGCGTGATACCGACGATGGCCACACGCTGCTTTA
CATCGACCTGCATCTGGTCAATGAAGTGACCAGCCCGCAGGCGTTCGAAGGACTGCGCATCAGCCGGTTGCACCCGTGGC
GGCCCGCCTCAGTGCTGGCCACCGAAGACCATAACGTGCCGACCGCCAACCGCGCCGCCGGCATTACCGATGACATCGCG
CGGCGTCAGGTCGAGGCGCTGCGCAGCAACTGCGCGCGCTTCGGCATTACCGAATACCCGATGCAGGACGCGCGTCAGGG
CATCCTGCACGTGATCGGGCCGGAGCAGGGGGCCACGCTGCCAGGCATGACGATCGTCTGCGGCGATTCGCATACCACCA
CGCACGGCGCATTCGCCACGCTGGCATTCGGTATCGGGACGTCCGAAATCGAACACGTGCTGGCCACGCAATGCCTGATC
GTCAAAAAGATGAAGACGATGCGCGTGCTGGTGGAGGGCGACCTGTCGCCCGGCGTGACTTCCAAGGACGTGGCGCTTGC
GCTGATCGGCCGTATCGGCACCGCGGGCGCCACCGGGTATGCCATCGAATTTGCCGGCTCGGCCATCTCTGCGATGAGCA
TGGATGCACGCATGACCCTGTGCAACATGGTCATCGAAGCCGGAGCACGCTGCGGCATGGTCGCCGTGGATGACAAAACC
ATCAACTACCTTCGCGGGCGGGCGCGTGCGCCGCAGGGCGCGCAATGGGACGCCGCGCAGGCGTACTGGCGCACGCTGCG
CAGCGATGAAGGGGCGCAGTTCGATCGGACTATCGCACTCGACGCGACCACGATTCGCCCACACGTCACCTGGGGCACCT
CGCCCGAGATGGTGGTCACCATCGACGGCCGTGTGCCCGACCCGGCCGAGGAAAGCGACCCCATCAAGCGGCAAGGCATC
ACCCGGGCGCTTACCTACATGGGACTCGAGCCGGGCACACCGCTGCGCGACATCGCACTCGACAAGATTTTCATCGGCTC
GTGCACGAATGCACGTATCGAAGATCTGCGTGCCGCGGCGCGTGTGGTGGCCGGCAAGCACGTTGCGGCCAACATCGTGC
AGGCGCTTGTGGTGCCGGGCTCCGGACTGGTCAAACGCCAGGCCGAGGCCGAAGGGCTCGACCGCATCTTCGTCGATGCC
GGTTTTGAATGGCGCGACCCGGGGTGTTCGATGTGCCTGGGCATGAACGACGACCGCCTGCAGCCGGGCGAGCGCTGCGC
GTCAACGTCCAATCGCAATTTTGAAGGGCGGCAGGGCGCTGGCGGGCGCACCCATCTGGTCAGCCCGCCAATGGCGGCTG
CCGCAGCAATCGCGGGGCATTTCGTTGCCGTCAGCGAACTTGGCGCCGATGTTTTGGGGAGGAACAACGATGCAGGCTTT
TGA

Upstream 100 bases:

>100_bases
TGGGTGAGCACACCGACGAGGTGCTGCACGCGCTCGGACTCGACGCTGCCGCGCTCGACAGCCTGCGCGCCGAAGGCATC
ATCTGACAGCGGGATCGCAC

Downstream 100 bases:

>100_bases
TTCGATTGACGGACTGGTGGTGCCGCTTGACCGCGCCAATATCGACACCGACGCCATCCTGCCAAAGCAATACCTGAAGA
TGATCGGCAAGACCGGTTTT

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MTPQTLFAKLWQRHAVRDTDDGHTLLYIDLHLVNEVTSPQAFEGLRISRLHPWRPASVLATEDHNVPTANRAAGITDDIA
RRQVEALRSNCARFGITEYPMQDARQGILHVIGPEQGATLPGMTIVCGDSHTTTHGAFATLAFGIGTSEIEHVLATQCLI
VKKMKTMRVLVEGDLSPGVTSKDVALALIGRIGTAGATGYAIEFAGSAISAMSMDARMTLCNMVIEAGARCGMVAVDDKT
INYLRGRARAPQGAQWDAAQAYWRTLRSDEGAQFDRTIALDATTIRPHVTWGTSPEMVVTIDGRVPDPAEESDPIKRQGI
TRALTYMGLEPGTPLRDIALDKIFIGSCTNARIEDLRAAARVVAGKHVAANIVQALVVPGSGLVKRQAEAEGLDRIFVDA
GFEWRDPGCSMCLGMNDDRLQPGERCASTSNRNFEGRQGAGGRTHLVSPPMAAAAAIAGHFVAVSELGADVLGRNNDAGF

Sequences:

>Translated_480_residues
MTPQTLFAKLWQRHAVRDTDDGHTLLYIDLHLVNEVTSPQAFEGLRISRLHPWRPASVLATEDHNVPTANRAAGITDDIA
RRQVEALRSNCARFGITEYPMQDARQGILHVIGPEQGATLPGMTIVCGDSHTTTHGAFATLAFGIGTSEIEHVLATQCLI
VKKMKTMRVLVEGDLSPGVTSKDVALALIGRIGTAGATGYAIEFAGSAISAMSMDARMTLCNMVIEAGARCGMVAVDDKT
INYLRGRARAPQGAQWDAAQAYWRTLRSDEGAQFDRTIALDATTIRPHVTWGTSPEMVVTIDGRVPDPAEESDPIKRQGI
TRALTYMGLEPGTPLRDIALDKIFIGSCTNARIEDLRAAARVVAGKHVAANIVQALVVPGSGLVKRQAEAEGLDRIFVDA
GFEWRDPGCSMCLGMNDDRLQPGERCASTSNRNFEGRQGAGGRTHLVSPPMAAAAAIAGHFVAVSELGADVLGRNNDAGF
>Mature_479_residues
TPQTLFAKLWQRHAVRDTDDGHTLLYIDLHLVNEVTSPQAFEGLRISRLHPWRPASVLATEDHNVPTANRAAGITDDIAR
RQVEALRSNCARFGITEYPMQDARQGILHVIGPEQGATLPGMTIVCGDSHTTTHGAFATLAFGIGTSEIEHVLATQCLIV
KKMKTMRVLVEGDLSPGVTSKDVALALIGRIGTAGATGYAIEFAGSAISAMSMDARMTLCNMVIEAGARCGMVAVDDKTI
NYLRGRARAPQGAQWDAAQAYWRTLRSDEGAQFDRTIALDATTIRPHVTWGTSPEMVVTIDGRVPDPAEESDPIKRQGIT
RALTYMGLEPGTPLRDIALDKIFIGSCTNARIEDLRAAARVVAGKHVAANIVQALVVPGSGLVKRQAEAEGLDRIFVDAG
FEWRDPGCSMCLGMNDDRLQPGERCASTSNRNFEGRQGAGGRTHLVSPPMAAAAAIAGHFVAVSELGADVLGRNNDAGF

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=389, Percent_Identity=27.7634961439589, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI41352693, Length=401, Percent_Identity=26.6832917705736, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI8659555, Length=462, Percent_Identity=25.3246753246753, Blast_Score=105, Evalue=8e-23,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=59.4827586206897, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=27.4238227146814, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI87081781, Length=357, Percent_Identity=26.0504201680672, Blast_Score=89, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI25149337, Length=392, Percent_Identity=27.8061224489796, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI32564738, Length=392, Percent_Identity=27.8061224489796, Blast_Score=125, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI25149342, Length=305, Percent_Identity=28.5245901639344, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17568399, Length=470, Percent_Identity=25.1063829787234, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=61.1464968152866, Blast_Score=582, Evalue=1e-167,
Organism=Saccharomyces cerevisiae, GI6320440, Length=415, Percent_Identity=25.5421686746988, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6323335, Length=390, Percent_Identity=27.6923076923077, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6322261, Length=382, Percent_Identity=27.7486910994764, Blast_Score=123, Evalue=5e-29,
Organism=Drosophila melanogaster, GI161076999, Length=391, Percent_Identity=27.6214833759591, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI281365315, Length=391, Percent_Identity=27.6214833759591, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17864292, Length=391, Percent_Identity=27.6214833759591, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI28571643, Length=391, Percent_Identity=27.6214833759591, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24645686, Length=489, Percent_Identity=25.5623721881391, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI17137564, Length=386, Percent_Identity=25.9067357512953, Blast_Score=99, Evalue=8e-21,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 51457; Mature: 51326

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPQTLFAKLWQRHAVRDTDDGHTLLYIDLHLVNEVTSPQAFEGLRISRLHPWRPASVLA
CCHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEHHCCCCCHHHCCCEEECCCCCCCCEEEE
TEDHNVPTANRAAGITDDIARRQVEALRSNCARFGITEYPMQDARQGILHVIGPEQGATL
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCEEEEECCCCCCCC
PGMTIVCGDSHTTTHGAFATLAFGIGTSEIEHVLATQCLIVKKMKTMRVLVEGDLSPGVT
CCEEEEECCCCCCCCHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC
SKDVALALIGRIGTAGATGYAIEFAGSAISAMSMDARMTLCNMVIEAGARCGMVAVDDKT
CHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHH
INYLRGRARAPQGAQWDAAQAYWRTLRSDEGAQFDRTIALDATTIRPHVTWGTSPEMVVT
HHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCEEEECCCCCEEEE
IDGRVPDPAEESDPIKRQGITRALTYMGLEPGTPLRDIALDKIFIGSCTNARIEDLRAAA
ECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCEEEECCCCCHHHHHHHHHH
RVVAGKHVAANIVQALVVPGSGLVKRQAEAEGLDRIFVDAGFEWRDPGCSMCLGMNDDRL
HHHHCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCCCCC
QPGERCASTSNRNFEGRQGAGGRTHLVSPPMAAAAAIAGHFVAVSELGADVLGRNNDAGF
CCHHHHHCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCHHHHCCCCCCCC
>Mature Secondary Structure 
TPQTLFAKLWQRHAVRDTDDGHTLLYIDLHLVNEVTSPQAFEGLRISRLHPWRPASVLA
CHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEHHCCCCCHHHCCCEEECCCCCCCCEEEE
TEDHNVPTANRAAGITDDIARRQVEALRSNCARFGITEYPMQDARQGILHVIGPEQGATL
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCEEEEECCCCCCCC
PGMTIVCGDSHTTTHGAFATLAFGIGTSEIEHVLATQCLIVKKMKTMRVLVEGDLSPGVT
CCEEEEECCCCCCCCHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC
SKDVALALIGRIGTAGATGYAIEFAGSAISAMSMDARMTLCNMVIEAGARCGMVAVDDKT
CHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHH
INYLRGRARAPQGAQWDAAQAYWRTLRSDEGAQFDRTIALDATTIRPHVTWGTSPEMVVT
HHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCEEEECCCCCEEEE
IDGRVPDPAEESDPIKRQGITRALTYMGLEPGTPLRDIALDKIFIGSCTNARIEDLRAAA
ECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCEEEECCCCCHHHHHHHHHH
RVVAGKHVAANIVQALVVPGSGLVKRQAEAEGLDRIFVDAGFEWRDPGCSMCLGMNDDRL
HHHHCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCCCCC
QPGERCASTSNRNFEGRQGAGGRTHLVSPPMAAAAAIAGHFVAVSELGADVLGRNNDAGF
CCHHHHHCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA