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The map label for this gene is radA [H]

Identifier: 187928334

GI number: 187928334

Start: 1333268

End: 1334638

Strand: Direct

Name: radA [H]

Synonym: Rpic_1245

Alternate gene names: 187928334

Gene position: 1333268-1334638 (Clockwise)

Preceding gene: 187928333

Following gene: 187928335

Centisome position: 33.82

GC content: 66.08

Gene sequence:

>1371_bases
TTGGCCAAGAGCAAGACGGTCTATACGTGCACCGAATGTGGTGGCACGGCACCGAAGTGGGCGGGGCAGTGCCCGAATTG
CCAGCAGTGGAACACGCTGGTGGAAACCGTCGCGCAGCCGACGTCCGGCGCCGGCGCGCGCTTCCAGTCGCTCGCCTCCA
GCGCGACGGTGCGCAAGCTGTCGGACATCGACGCGGTGGATGTGCCGCGCTTCTCCAGCGGCATCGACGAATTTGACCGC
GTACTGGGCGGTGGCCTGGTCAGCGGCGGTGTTGTGCTGATCGGCGGCGATCCCGGGATCGGCAAATCGACGCTGTTGCT
GCAGGCGCTGTCCAACCTGTCGGCCGATCGGCGGGTGTTGTACGTGAGCGGCGAGGAGTCCGGCTCGCAGATCGCGCTTC
GTGCGCGTCGCCTGGGCATCGAAAGCCCGAACCTTGCGCTGCTGGCGGAGATCCAGCTGGAGCGCATCCAGGCGACGATA
GAAACGGAAAAGCCGGAAGTCGTCGTCATCGATTCGATCCAGACGCTGTATTCCGAGGCGCTGACCTCGGCGCCAGGCTC
CGTGGCCCAGGTGCGGGAATGCGCGGCGCAGTTGACGCGCATTGCCAAGCGGCTGGATGTGACGACCATTCTCGTTGGCC
ATGTGACCAAGGAAGGCGCGCTGGCAGGCCCCCGTGTGCTCGAACACATTGTCGATACGGTGCTGTATTTCGAGGGCGAT
ACGCATTCCGCCTACCGGCTGGTGCGGGCGTTCAAGAACCGTTTCGGCGCCGTCAACGAGCTGGGCGTGTTTGCGATGAC
GGAGCGCGGCCTGCGTGGCGTGGCGAATCCGTCGGCGCTCTTCCTGTCGCAGCACGAACAGGTGGTGCCGGGCTCGTGCG
TGCTGGTGACGCAGGAGGGCACGCGGCCGCTGCTGGTGGAAATCCAGGCGCTGGTGGATACGGCCAACGTGCCGAACCCG
CGCCGCCTGGCGGTGGGCCTGGAGCAGAATCGGCTGGCGATGTTGCTGGCGGTGCTGCACCGCCACGCCGGCATTGCGTG
CTTTGACCAGGACGTGTTCCTGAACGCGGTGGGCGGCGTGAAGATTACCGAGCCCGCGGCGGACTTGGCGGTGCTGTTGG
CGATCCATTCGTCGATGCGCAACAAGGCGCTGCCGCGCGGGCTGGTCGTGTTTGGGGAGATCGGCCTTGCCGGGGAAATC
CGCCCGACGCCGCGCGGTCAGGAGCGGTTGAAGGAAGCGGCCAAGCTGGGCTTCTCGATTGCCGTGATCCCGAAATCCAA
CGCCCCGAAGCAGGCAATCGATGGGCTGGAGGTCATTGCCGTCGAGCGCATTGAACAGGCCATCGATCGGGTGCGGGCGC
TCGAGGCTTGA

Upstream 100 bases:

>100_bases
CAGAGGCCGGCGACCTGGGCAAGGCTGCCTGAGCGCCGCATCGTTAATACAAAGATCAAGCGGCGGTCGCCCATGCGCGG
CGCCGTAAGCAGGGGGTTGT

Downstream 100 bases:

>100_bases
ACGTTCAAAATTTCACTACGGTATCGCTCGTGCCATTTGAAAATGCGTGTGCCGTTGCGGTGCGATAATCCGCCAACTTT
TCTCCACGACTCCTCTGTAC

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MAKSKTVYTCTECGGTAPKWAGQCPNCQQWNTLVETVAQPTSGAGARFQSLASSATVRKLSDIDAVDVPRFSSGIDEFDR
VLGGGLVSGGVVLIGGDPGIGKSTLLLQALSNLSADRRVLYVSGEESGSQIALRARRLGIESPNLALLAEIQLERIQATI
ETEKPEVVVIDSIQTLYSEALTSAPGSVAQVRECAAQLTRIAKRLDVTTILVGHVTKEGALAGPRVLEHIVDTVLYFEGD
THSAYRLVRAFKNRFGAVNELGVFAMTERGLRGVANPSALFLSQHEQVVPGSCVLVTQEGTRPLLVEIQALVDTANVPNP
RRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLAIHSSMRNKALPRGLVVFGEIGLAGEI
RPTPRGQERLKEAAKLGFSIAVIPKSNAPKQAIDGLEVIAVERIEQAIDRVRALEA

Sequences:

>Translated_456_residues
MAKSKTVYTCTECGGTAPKWAGQCPNCQQWNTLVETVAQPTSGAGARFQSLASSATVRKLSDIDAVDVPRFSSGIDEFDR
VLGGGLVSGGVVLIGGDPGIGKSTLLLQALSNLSADRRVLYVSGEESGSQIALRARRLGIESPNLALLAEIQLERIQATI
ETEKPEVVVIDSIQTLYSEALTSAPGSVAQVRECAAQLTRIAKRLDVTTILVGHVTKEGALAGPRVLEHIVDTVLYFEGD
THSAYRLVRAFKNRFGAVNELGVFAMTERGLRGVANPSALFLSQHEQVVPGSCVLVTQEGTRPLLVEIQALVDTANVPNP
RRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLAIHSSMRNKALPRGLVVFGEIGLAGEI
RPTPRGQERLKEAAKLGFSIAVIPKSNAPKQAIDGLEVIAVERIEQAIDRVRALEA
>Mature_455_residues
AKSKTVYTCTECGGTAPKWAGQCPNCQQWNTLVETVAQPTSGAGARFQSLASSATVRKLSDIDAVDVPRFSSGIDEFDRV
LGGGLVSGGVVLIGGDPGIGKSTLLLQALSNLSADRRVLYVSGEESGSQIALRARRLGIESPNLALLAEIQLERIQATIE
TEKPEVVVIDSIQTLYSEALTSAPGSVAQVRECAAQLTRIAKRLDVTTILVGHVTKEGALAGPRVLEHIVDTVLYFEGDT
HSAYRLVRAFKNRFGAVNELGVFAMTERGLRGVANPSALFLSQHEQVVPGSCVLVTQEGTRPLLVEIQALVDTANVPNPR
RLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLAIHSSMRNKALPRGLVVFGEIGLAGEIR
PTPRGQERLKEAAKLGFSIAVIPKSNAPKQAIDGLEVIAVERIEQAIDRVRALEA

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=454, Percent_Identity=57.2687224669604, Blast_Score=528, Evalue=1e-151,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 48578; Mature: 48446

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSKTVYTCTECGGTAPKWAGQCPNCQQWNTLVETVAQPTSGAGARFQSLASSATVRKL
CCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
SDIDAVDVPRFSSGIDEFDRVLGGGLVSGGVVLIGGDPGIGKSTLLLQALSNLSADRRVL
HCCCCCCCCCHHCCHHHHHHHHCCCEEECCEEEECCCCCCCHHHHHHHHHHCCCCCCEEE
YVSGEESGSQIALRARRLGIESPNLALLAEIQLERIQATIETEKPEVVVIDSIQTLYSEA
EEECCCCCCHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHH
LTSAPGSVAQVRECAAQLTRIAKRLDVTTILVGHVTKEGALAGPRVLEHIVDTVLYFEGD
HHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHEEECCC
THSAYRLVRAFKNRFGAVNELGVFAMTERGLRGVANPSALFLSQHEQVVPGSCVLVTQEG
CHHHHHHHHHHHHHCCCHHHCCHHEEHHHCCCCCCCCHHHHHCCCCCCCCCCEEEEECCC
TRPLLVEIQALVDTANVPNPRRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGV
CCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEECCCHHEEECCCC
KITEPAADLAVLLAIHSSMRNKALPRGLVVFGEIGLAGEIRPTPRGQERLKEAAKLGFSI
EEECCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEE
AVIPKSNAPKQAIDGLEVIAVERIEQAIDRVRALEA
EEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKSKTVYTCTECGGTAPKWAGQCPNCQQWNTLVETVAQPTSGAGARFQSLASSATVRKL
CCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
SDIDAVDVPRFSSGIDEFDRVLGGGLVSGGVVLIGGDPGIGKSTLLLQALSNLSADRRVL
HCCCCCCCCCHHCCHHHHHHHHCCCEEECCEEEECCCCCCCHHHHHHHHHHCCCCCCEEE
YVSGEESGSQIALRARRLGIESPNLALLAEIQLERIQATIETEKPEVVVIDSIQTLYSEA
EEECCCCCCHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHH
LTSAPGSVAQVRECAAQLTRIAKRLDVTTILVGHVTKEGALAGPRVLEHIVDTVLYFEGD
HHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHEEECCC
THSAYRLVRAFKNRFGAVNELGVFAMTERGLRGVANPSALFLSQHEQVVPGSCVLVTQEG
CHHHHHHHHHHHHHCCCHHHCCHHEEHHHCCCCCCCCHHHHHCCCCCCCCCCEEEEECCC
TRPLLVEIQALVDTANVPNPRRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGV
CCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEECCCHHEEECCCC
KITEPAADLAVLLAIHSSMRNKALPRGLVVFGEIGLAGEIRPTPRGQERLKEAAKLGFSI
EEECCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEE
AVIPKSNAPKQAIDGLEVIAVERIEQAIDRVRALEA
EEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]