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The map label for this gene is yhcA [H]

Identifier: 187928327

GI number: 187928327

Start: 1326084

End: 1327541

Strand: Direct

Name: yhcA [H]

Synonym: Rpic_1238

Alternate gene names: 187928327

Gene position: 1326084-1327541 (Clockwise)

Preceding gene: 187928326

Following gene: 187928329

Centisome position: 33.64

GC content: 65.09

Gene sequence:

>1458_bases
ATGACGACATTCACGCCCACCCTGGAGGCGCTGCGCGCGCGTTACGGCGAGCGCTTCAAATGGCTCGTTCTCTTCACGCT
GATGGTGGGTGCGGTGTCGTCCGTCATCTCCGCCACGATCGTGAACGTGGCGATTCCCGACCTAAGCCGGCATTTCGTGC
TCGGGCAGGAACGCGCGCAGTGGGTATCGGCCAGCTTCATGGTGGCGATGACGTTGTCGATGCTGCTGACACCGTGGCTG
CTGCTGCGTTTCGGGCTGCGTCGCACGTTTATCGGCGCGTTGCTGCTGCTGGGCGTCGGCGGGCTGGCGGGTGGCCTGTC
GCCCAATTACGAAGTAATGATCGCCATGCGCGTCGTGGAAGGGATTGCGGCCGGCATCATGCAGCCGCTGCCCAACATCC
TGATCCTGCGCGTGTTTCCCGAGCGCGAGCAGGGCAAGGCGTTCGGCCTGTTCGGCTTTGGTGTTGTGCTCGCGCCGGCC
GTGGGCCCGAGCGTGGGCGGTTTCCTGGTGGAATTGTTCGGCTGGCGCTCGATCTTCTTCGTGGTGATGCCGTTCACGCT
GATCGGCTGGGCGATGGCGCGGCGCTTCATGGCCATCAACTCGTCGATGGCAGGCGAGCCCAAGCCGCTCGATTGGCGTG
GCCTGCTGCTAATCGGCGGTGCGACCGTCACGCTGCTCAACGGACTGGTCGCGCTGCATGGCGATGCTGCGCACGGCATA
GTGCTGATGCTCGTGTCGGGCGTGTGCCTGGCGGGCTTCGTGTTCTGGCAGCGGCGGGTGGAGTCGCCGTTGCTGAACCT
GCGGCTGTTCAGCTACCGGCAGTTTGCGATGGGGGCAGTCGTGGCTTTCATCTACGGGGCAGGGCTGTACGGTTCGACGT
ACCTTGTGCCCGTGTATATGCAGGTGGCGCTGGCCTATGCGCCGTCGCGCGCCGGGCTGGTGCTGCTGCCGGCCGGCCTC
GTGCTGGCCGTGACGATCATGCTGGCGGGGCGCCTGACCAATCGCATCGAGCCTTTCCGACTGGTGTCGTTCGGGCTGGC
GGCCCTGGCGCTGTCGTTTTTCCTGATGGCCACCAACACCCGGGCAACGAGCTATCTGCTGCTGATCGCCATCGCCGTCA
TCGGGCGGGTTGGGCTCGGTTTCGTGCTGCCATCGCTGAGCCTTGGCGCCATGCGCGGTGTTGATTTCACGTTGATCCCC
CAAGGCTCCAGCGCCGTCAATTTCCTGCGCCAATTAGGCGGGGCGATCGGTGTGTCGGCTACGGGCATTTTTCTGCAATG
GCGGCTCGCCACGCGGGGTATCGAAACGGTGCACGGTGCGGCAGTTGACCCCGAAGCTCGCATCCTGGCCTTTGACGAGA
CCTTCGTCTTTCTCGGGACGCTGTGCGCACTGGCCGTGCTGGCTGCATGGCGCATGCGCCGCCGCGAGCTGCCGTCCGCC
ATTCCTGCAGCGCAATAA

Upstream 100 bases:

>100_bases
CCTCGGCTGACGTGTCTTGCCCGGCCGGCGCTGGAGGAGGCGCCGGCCGGGTTTGCTCTGCCTGCACCCTGGTCGTCTCA
CACATCACGACAACGAGACC

Downstream 100 bases:

>100_bases
AAAACCGGGCACGGGGCCCGGTTCTTCGTTGGCGATTCGACGATCAGGTCGTTGCGCCGCTCTTCAGTTCTTCGACGAGG
TCGACGTACTTTTGCTTGGC

Product: EmrB/QacA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MTTFTPTLEALRARYGERFKWLVLFTLMVGAVSSVISATIVNVAIPDLSRHFVLGQERAQWVSASFMVAMTLSMLLTPWL
LLRFGLRRTFIGALLLLGVGGLAGGLSPNYEVMIAMRVVEGIAAGIMQPLPNILILRVFPEREQGKAFGLFGFGVVLAPA
VGPSVGGFLVELFGWRSIFFVVMPFTLIGWAMARRFMAINSSMAGEPKPLDWRGLLLIGGATVTLLNGLVALHGDAAHGI
VLMLVSGVCLAGFVFWQRRVESPLLNLRLFSYRQFAMGAVVAFIYGAGLYGSTYLVPVYMQVALAYAPSRAGLVLLPAGL
VLAVTIMLAGRLTNRIEPFRLVSFGLAALALSFFLMATNTRATSYLLLIAIAVIGRVGLGFVLPSLSLGAMRGVDFTLIP
QGSSAVNFLRQLGGAIGVSATGIFLQWRLATRGIETVHGAAVDPEARILAFDETFVFLGTLCALAVLAAWRMRRRELPSA
IPAAQ

Sequences:

>Translated_485_residues
MTTFTPTLEALRARYGERFKWLVLFTLMVGAVSSVISATIVNVAIPDLSRHFVLGQERAQWVSASFMVAMTLSMLLTPWL
LLRFGLRRTFIGALLLLGVGGLAGGLSPNYEVMIAMRVVEGIAAGIMQPLPNILILRVFPEREQGKAFGLFGFGVVLAPA
VGPSVGGFLVELFGWRSIFFVVMPFTLIGWAMARRFMAINSSMAGEPKPLDWRGLLLIGGATVTLLNGLVALHGDAAHGI
VLMLVSGVCLAGFVFWQRRVESPLLNLRLFSYRQFAMGAVVAFIYGAGLYGSTYLVPVYMQVALAYAPSRAGLVLLPAGL
VLAVTIMLAGRLTNRIEPFRLVSFGLAALALSFFLMATNTRATSYLLLIAIAVIGRVGLGFVLPSLSLGAMRGVDFTLIP
QGSSAVNFLRQLGGAIGVSATGIFLQWRLATRGIETVHGAAVDPEARILAFDETFVFLGTLCALAVLAAWRMRRRELPSA
IPAAQ
>Mature_484_residues
TTFTPTLEALRARYGERFKWLVLFTLMVGAVSSVISATIVNVAIPDLSRHFVLGQERAQWVSASFMVAMTLSMLLTPWLL
LRFGLRRTFIGALLLLGVGGLAGGLSPNYEVMIAMRVVEGIAAGIMQPLPNILILRVFPEREQGKAFGLFGFGVVLAPAV
GPSVGGFLVELFGWRSIFFVVMPFTLIGWAMARRFMAINSSMAGEPKPLDWRGLLLIGGATVTLLNGLVALHGDAAHGIV
LMLVSGVCLAGFVFWQRRVESPLLNLRLFSYRQFAMGAVVAFIYGAGLYGSTYLVPVYMQVALAYAPSRAGLVLLPAGLV
LAVTIMLAGRLTNRIEPFRLVSFGLAALALSFFLMATNTRATSYLLLIAIAVIGRVGLGFVLPSLSLGAMRGVDFTLIPQ
GSSAVNFLRQLGGAIGVSATGIFLQWRLATRGIETVHGAAVDPEARILAFDETFVFLGTLCALAVLAAWRMRRRELPSAI
PAAQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=421, Percent_Identity=28.2660332541568, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1789042, Length=429, Percent_Identity=27.2727272727273, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1790195, Length=291, Percent_Identity=29.2096219931272, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI87081983, Length=351, Percent_Identity=25.6410256410256, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788392, Length=419, Percent_Identity=25.5369928400955, Blast_Score=86, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323735, Length=324, Percent_Identity=24.3827160493827, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52158; Mature: 52027

Theoretical pI: Translated: 11.33; Mature: 11.33

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFTPTLEALRARYGERFKWLVLFTLMVGAVSSVISATIVNVAIPDLSRHFVLGQERAQ
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHH
WVSASFMVAMTLSMLLTPWLLLRFGLRRTFIGALLLLGVGGLAGGLSPNYEVMIAMRVVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCHHEEHHHHHHH
GIAAGIMQPLPNILILRVFPEREQGKAFGLFGFGVVLAPAVGPSVGGFLVELFGWRSIFF
HHHHHHHHCCCCEEEEEECCCCCCCCEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
VVMPFTLIGWAMARRFMAINSSMAGEPKPLDWRGLLLIGGATVTLLNGLVALHGDAAHGI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCHHHH
VLMLVSGVCLAGFVFWQRRVESPLLNLRLFSYRQFAMGAVVAFIYGAGLYGSTYLVPVYM
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
QVALAYAPSRAGLVLLPAGLVLAVTIMLAGRLTNRIEPFRLVSFGLAALALSFFLMATNT
HHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
RATSYLLLIAIAVIGRVGLGFVLPSLSLGAMRGVDFTLIPQGSSAVNFLRQLGGAIGVSA
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEECCCCHHHHHHHHHCCCCCCCE
TGIFLQWRLATRGIETVHGAAVDPEARILAFDETFVFLGTLCALAVLAAWRMRRRELPSA
EEEEEEEEHHHCCHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IPAAQ
CCCCC
>Mature Secondary Structure 
TTFTPTLEALRARYGERFKWLVLFTLMVGAVSSVISATIVNVAIPDLSRHFVLGQERAQ
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHH
WVSASFMVAMTLSMLLTPWLLLRFGLRRTFIGALLLLGVGGLAGGLSPNYEVMIAMRVVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCHHEEHHHHHHH
GIAAGIMQPLPNILILRVFPEREQGKAFGLFGFGVVLAPAVGPSVGGFLVELFGWRSIFF
HHHHHHHHCCCCEEEEEECCCCCCCCEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
VVMPFTLIGWAMARRFMAINSSMAGEPKPLDWRGLLLIGGATVTLLNGLVALHGDAAHGI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCHHHH
VLMLVSGVCLAGFVFWQRRVESPLLNLRLFSYRQFAMGAVVAFIYGAGLYGSTYLVPVYM
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
QVALAYAPSRAGLVLLPAGLVLAVTIMLAGRLTNRIEPFRLVSFGLAALALSFFLMATNT
HHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
RATSYLLLIAIAVIGRVGLGFVLPSLSLGAMRGVDFTLIPQGSSAVNFLRQLGGAIGVSA
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEECCCCHHHHHHHHHCCCCCCCE
TGIFLQWRLATRGIETVHGAAVDPEARILAFDETFVFLGTLCALAVLAAWRMRRRELPSA
EEEEEEEEHHHCCHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IPAAQ
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]