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The map label for this gene is dnaB [H]

Identifier: 187928275

GI number: 187928275

Start: 1272614

End: 1274002

Strand: Direct

Name: dnaB [H]

Synonym: Rpic_1186

Alternate gene names: 187928275

Gene position: 1272614-1274002 (Clockwise)

Preceding gene: 187928274

Following gene: 187928276

Centisome position: 32.28

GC content: 60.84

Gene sequence:

>1389_bases
ATGAACGCGCCCTCCGATCCCCAGCTCGATTCCCTCAAGGTGCCCCCGCATTCCATCGAGGCCGAACAGTCGGTGCTCGG
CGGGTTGCTGTTGGATAACGCCGCCTGGGATCGCATTGCCGATTTCATCAACGAGCACGATTTCTACCGCTACGACCACC
GGCTGATCTTCCACAATATCGGCAAGTTGATCTCGCAGGCCAAGCCGGCTGACGTGATCACGGTGTACGAGCAACTGCAG
GCCGCTGGCAAGGCGGAAGAGGTGGGTGGCCTGGCGTACCTGAACGCGCTGGCCCAAAACACGCCGAGCGCCGCCAATAT
CCGTCGCTATGCGGAGATCGTTCGCGACCGCGGCGTGCTGCGCCAACTCGTGACGATCGCCGACGAGATTTCCGCTGGTG
CCTTCAACCCGCAGGGGCGCGACGTGCGCCAGTTGTTGGACGAGGCCGAATCCAAGGTGTTCGCGATTGCCGAAGAGGGC
TCGCGTGGGCAGAAGGGCTTCCTGGAAATTCAGCCGCTGCTGACGCAGGTGGTCGAGCGCATCGATGAGCTGTACCACCG
CGATAACCAGAGCGACATTACCGGTGTGCCGACGGGTTTCGTTGACCTCGATCGCATGACCAGCGGTATGCAGGGCGGCG
ACCTGATCATCGTGGCCGGTCGCCCATCGATGGGTAAGACGGCATTCTCCCTGAACATCGGCGAACACGTGGCGGTGGAG
CAGGGCCTGCCGGTGGCTGTGTTCTCAATGGAAATGGCGGGTACGCAGCTCGCCATGCGTATGCTGGGTTCGGTCGGTCG
ATTGGATCAGCACCGCCTGCGCACCGGCCGACTGCTCGACGAGGATTGGCCGCGCCTGACGCATGCCATCCAGAAGATGA
ACGACGCGCAGCTCTTCATCGACGAAACCCCTGCGTTGAACCCGATGGAGTTGCGTGCACGTTCGCGCCGGCTTGCACGC
CAGTGTGGACAGCTCGGCCTGATCGTCATCGACTACCTGCAGCTGATGTCGGGCTCGGGCAGCGGTGAGAACCGTGCGAC
CGAAATTTCGGAAATCTCGCGATCGCTCAAGGGTCTGGCGAAGGAACTCAACTGCCCCGTGATCGCATTGTCGCAGTTGA
ACCGAAGCTTGGAGCAGCGCCCGAACAAGCGTCCTGTGATGTCGGACTTGCGCGAATCCGGTGCTATCGAACAGGACGCC
GACGTGATCCTGTTCATCTATCGCGATCAGGTCTACAACCCCGATTCTCCGGACAAGGGTACGGCCGAAATCATCATTGG
TAAGCAGCGTAACGGCCCGATTGGGACCGTTCGCCTTACGTTCCTGGGTGAATACACGAAGTTCGACAACTTCACCGGGG
GCAATGCCTTCTTTGACAACGACACGTAA

Upstream 100 bases:

>100_bases
CGCGAGTCAAGAAGGCAGGGACGAAAGTCTCTGCCTTTTTTGTTGTCCTTTGTGTCAGGGGTTCAAATTGAAGACTGAGC
AGCGCCGTTATAATCCCGCC

Downstream 100 bases:

>100_bases
CGTGTAATTCGTGTCGGCCTGCCACAACGTGCCACGCATGTCGCGAGGCTGGTTCCGGTAAAATATTGCGTTTTAATGAC
AGCGCCGGCGGTGATCCCGT

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MNAPSDPQLDSLKVPPHSIEAEQSVLGGLLLDNAAWDRIADFINEHDFYRYDHRLIFHNIGKLISQAKPADVITVYEQLQ
AAGKAEEVGGLAYLNALAQNTPSAANIRRYAEIVRDRGVLRQLVTIADEISAGAFNPQGRDVRQLLDEAESKVFAIAEEG
SRGQKGFLEIQPLLTQVVERIDELYHRDNQSDITGVPTGFVDLDRMTSGMQGGDLIIVAGRPSMGKTAFSLNIGEHVAVE
QGLPVAVFSMEMAGTQLAMRMLGSVGRLDQHRLRTGRLLDEDWPRLTHAIQKMNDAQLFIDETPALNPMELRARSRRLAR
QCGQLGLIVIDYLQLMSGSGSGENRATEISEISRSLKGLAKELNCPVIALSQLNRSLEQRPNKRPVMSDLRESGAIEQDA
DVILFIYRDQVYNPDSPDKGTAEIIIGKQRNGPIGTVRLTFLGEYTKFDNFTGGNAFFDNDT

Sequences:

>Translated_462_residues
MNAPSDPQLDSLKVPPHSIEAEQSVLGGLLLDNAAWDRIADFINEHDFYRYDHRLIFHNIGKLISQAKPADVITVYEQLQ
AAGKAEEVGGLAYLNALAQNTPSAANIRRYAEIVRDRGVLRQLVTIADEISAGAFNPQGRDVRQLLDEAESKVFAIAEEG
SRGQKGFLEIQPLLTQVVERIDELYHRDNQSDITGVPTGFVDLDRMTSGMQGGDLIIVAGRPSMGKTAFSLNIGEHVAVE
QGLPVAVFSMEMAGTQLAMRMLGSVGRLDQHRLRTGRLLDEDWPRLTHAIQKMNDAQLFIDETPALNPMELRARSRRLAR
QCGQLGLIVIDYLQLMSGSGSGENRATEISEISRSLKGLAKELNCPVIALSQLNRSLEQRPNKRPVMSDLRESGAIEQDA
DVILFIYRDQVYNPDSPDKGTAEIIIGKQRNGPIGTVRLTFLGEYTKFDNFTGGNAFFDNDT
>Mature_462_residues
MNAPSDPQLDSLKVPPHSIEAEQSVLGGLLLDNAAWDRIADFINEHDFYRYDHRLIFHNIGKLISQAKPADVITVYEQLQ
AAGKAEEVGGLAYLNALAQNTPSAANIRRYAEIVRDRGVLRQLVTIADEISAGAFNPQGRDVRQLLDEAESKVFAIAEEG
SRGQKGFLEIQPLLTQVVERIDELYHRDNQSDITGVPTGFVDLDRMTSGMQGGDLIIVAGRPSMGKTAFSLNIGEHVAVE
QGLPVAVFSMEMAGTQLAMRMLGSVGRLDQHRLRTGRLLDEDWPRLTHAIQKMNDAQLFIDETPALNPMELRARSRRLAR
QCGQLGLIVIDYLQLMSGSGSGENRATEISEISRSLKGLAKELNCPVIALSQLNRSLEQRPNKRPVMSDLRESGAIEQDA
DVILFIYRDQVYNPDSPDKGTAEIIIGKQRNGPIGTVRLTFLGEYTKFDNFTGGNAFFDNDT

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=452, Percent_Identity=55.3097345132743, Blast_Score=499, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51070; Mature: 51070

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPSDPQLDSLKVPPHSIEAEQSVLGGLLLDNAAWDRIADFINEHDFYRYDHRLIFHNI
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
GKLISQAKPADVITVYEQLQAAGKAEEVGGLAYLNALAQNTPSAANIRRYAEIVRDRGVL
HHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RQLVTIADEISAGAFNPQGRDVRQLLDEAESKVFAIAEEGSRGQKGFLEIQPLLTQVVER
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHH
IDELYHRDNQSDITGVPTGFVDLDRMTSGMQGGDLIIVAGRPSMGKTAFSLNIGEHVAVE
HHHHHHCCCCCCCCCCCCCCEEHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCCHHHHH
QGLPVAVFSMEMAGTQLAMRMLGSVGRLDQHRLRTGRLLDEDWPRLTHAIQKMNDAQLFI
CCCCEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEE
DETPALNPMELRARSRRLARQCGQLGLIVIDYLQLMSGSGSGENRATEISEISRSLKGLA
ECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
KELNCPVIALSQLNRSLEQRPNKRPVMSDLRESGAIEQDADVILFIYRDQVYNPDSPDKG
HHCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCC
TAEIIIGKQRNGPIGTVRLTFLGEYTKFDNFTGGNAFFDNDT
CEEEEEECCCCCCEEEEEEEEEECHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNAPSDPQLDSLKVPPHSIEAEQSVLGGLLLDNAAWDRIADFINEHDFYRYDHRLIFHNI
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
GKLISQAKPADVITVYEQLQAAGKAEEVGGLAYLNALAQNTPSAANIRRYAEIVRDRGVL
HHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RQLVTIADEISAGAFNPQGRDVRQLLDEAESKVFAIAEEGSRGQKGFLEIQPLLTQVVER
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHH
IDELYHRDNQSDITGVPTGFVDLDRMTSGMQGGDLIIVAGRPSMGKTAFSLNIGEHVAVE
HHHHHHCCCCCCCCCCCCCCEEHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCCHHHHH
QGLPVAVFSMEMAGTQLAMRMLGSVGRLDQHRLRTGRLLDEDWPRLTHAIQKMNDAQLFI
CCCCEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEE
DETPALNPMELRARSRRLARQCGQLGLIVIDYLQLMSGSGSGENRATEISEISRSLKGLA
ECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
KELNCPVIALSQLNRSLEQRPNKRPVMSDLRESGAIEQDADVILFIYRDQVYNPDSPDKG
HHCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCC
TAEIIIGKQRNGPIGTVRLTFLGEYTKFDNFTGGNAFFDNDT
CEEEEEECCCCCCEEEEEEEEEECHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]