| Definition | Ralstonia pickettii 12J chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010682 |
| Length | 3,942,557 |
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The map label for this gene is ispG [H]
Identifier: 187928152
GI number: 187928152
Start: 1125040
End: 1126326
Strand: Direct
Name: ispG [H]
Synonym: Rpic_1059
Alternate gene names: 187928152
Gene position: 1125040-1126326 (Clockwise)
Preceding gene: 187928151
Following gene: 187928153
Centisome position: 28.54
GC content: 64.34
Gene sequence:
>1287_bases ATGGAAATCTCCACGCCTCTCGATTGCGCTCCAGCCTTGGTGGGCCCGCTGCCGCGCCGGAACTCGCGCGCCGCGCAAAT CCGCTGGGGTGATCGTGTCGTGCACGTCGGTGGCGACGCGCCGGTCTGCGTGCAATCGATGACGAATACCGACACTTCGG ACGCCATCGGTACCGCAATCCAGGTAAAGGAGCTGGCGCGTGCCGGCTCTGAGTTGGTGCGCATCACAGTGGATACCCCG GCCGCCGCGGCCGCGGTGCCTGCCATCCGCGAGCAGCTCGACCGCATGGGTGTGGATGTGCCGCTTGTCGGCGATTTCCA CTACAACGGCCACAAGCTGCTGCAGGACTATCCGGAGTGCGCGCAGGCGCTATCCAAGTACCGGATCAACCCCGGCAACG TCGGGCAGGGTGCCAAGCGCGACACGCAGTTCGCGCAGATGATCGAGATTGCTTGCCGCTATGACAAGCCGGTGCGTATC GGCGTGAACTGGGGCAGCCTCGATCAGGATCTGCTTGCGCGCATCATGGACGAGAACGCGCAACGTGCAACCCCCTGGGA TGCCCGCGCCGTGATGGTCGAGGCGCTGATCACCTCCGCGATCCAGTCCGCGCAAAAGGCTGAAGAACTGGGCTTGCCAG GCAGCCAGATCATCCTGTCGTGCAAGGTGTCGGCGGTGCAGGATCTGATTGCTGTGTACCGCGAGTTGGCGCGTCGCTGC CATTACGCGCTGCACCTGGGCCTGACCGAGGCCGGCATGGGGAGCAAGGGTATCGTCGCTTCCACGGCGGCGTTGTCGGT GCTGCTGCAGGAAGGCATCGGCGACACGATCCGCATTTCGCTCACGCCGGAACCGGGTGCGCCGCGCGAGAAGGAAGTCA TCGTCGCGCAAGAGATTCTGCAGACCATGGGCCTGCGCAATTTCACGCCCATGGTGATCGCGTGCCCGGGTTGCGGGCGC ACGACCAGCACGGTGTTCCAGGAACTGGCGGCGCGCATCCAGTCGTATTTGCGCGAGCAGATGCCGGTGTGGAAGGAGCA GTACCCCGGCGTCGAAGAGATGAATGTGGCCGTGATGGGCTGCATCGTCAACGGCCCCGGAGAGAGCAAGCACGCCAATA TCGGCATTTCGCTGCCAGGCACGGGCGAGTCGCCGGCGGCGCCGGTTTTCGTTGACGGCGTGAAGGTGAAAACACTGCGC GGCGAGCGCATCACAGAAGAATTCCAGGCGATCGTCGACGAGTACGTGCGGACGCATTACGGCGCACAGACTCCTGCGAC GGTCTAA
Upstream 100 bases:
>100_bases ATCTGCAGAAGTACGCCCGCAATCGCGTGGCACGGGCCACGTTGCCCTGAACCGTTTTGTAATTGGCGCGGTGCCTGTAG GCAGCGCGCCCTGTCTGATC
Downstream 100 bases:
>100_bases AGCGACATCAGGCTTCGGAAACCAAAGCCGAAGCACGAAACGACCGGCCGGCTCTGCATGAAGGTAGATGCGAGAAGTCG GCTTTCAACGTGACATCATG
Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
Products: NA
Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]
Number of amino acids: Translated: 428; Mature: 428
Protein sequence:
>428_residues MEISTPLDCAPALVGPLPRRNSRAAQIRWGDRVVHVGGDAPVCVQSMTNTDTSDAIGTAIQVKELARAGSELVRITVDTP AAAAAVPAIREQLDRMGVDVPLVGDFHYNGHKLLQDYPECAQALSKYRINPGNVGQGAKRDTQFAQMIEIACRYDKPVRI GVNWGSLDQDLLARIMDENAQRATPWDARAVMVEALITSAIQSAQKAEELGLPGSQIILSCKVSAVQDLIAVYRELARRC HYALHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVIVAQEILQTMGLRNFTPMVIACPGCGR TTSTVFQELAARIQSYLREQMPVWKEQYPGVEEMNVAVMGCIVNGPGESKHANIGISLPGTGESPAAPVFVDGVKVKTLR GERITEEFQAIVDEYVRTHYGAQTPATV
Sequences:
>Translated_428_residues MEISTPLDCAPALVGPLPRRNSRAAQIRWGDRVVHVGGDAPVCVQSMTNTDTSDAIGTAIQVKELARAGSELVRITVDTP AAAAAVPAIREQLDRMGVDVPLVGDFHYNGHKLLQDYPECAQALSKYRINPGNVGQGAKRDTQFAQMIEIACRYDKPVRI GVNWGSLDQDLLARIMDENAQRATPWDARAVMVEALITSAIQSAQKAEELGLPGSQIILSCKVSAVQDLIAVYRELARRC HYALHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVIVAQEILQTMGLRNFTPMVIACPGCGR TTSTVFQELAARIQSYLREQMPVWKEQYPGVEEMNVAVMGCIVNGPGESKHANIGISLPGTGESPAAPVFVDGVKVKTLR GERITEEFQAIVDEYVRTHYGAQTPATV >Mature_428_residues MEISTPLDCAPALVGPLPRRNSRAAQIRWGDRVVHVGGDAPVCVQSMTNTDTSDAIGTAIQVKELARAGSELVRITVDTP AAAAAVPAIREQLDRMGVDVPLVGDFHYNGHKLLQDYPECAQALSKYRINPGNVGQGAKRDTQFAQMIEIACRYDKPVRI GVNWGSLDQDLLARIMDENAQRATPWDARAVMVEALITSAIQSAQKAEELGLPGSQIILSCKVSAVQDLIAVYRELARRC HYALHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVIVAQEILQTMGLRNFTPMVIACPGCGR TTSTVFQELAARIQSYLREQMPVWKEQYPGVEEMNVAVMGCIVNGPGESKHANIGISLPGTGESPAAPVFVDGVKVKTLR GERITEEFQAIVDEYVRTHYGAQTPATV
Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]
COG id: COG0821
COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the ispG family [H]
Homologues:
Organism=Escherichia coli, GI1788863, Length=402, Percent_Identity=35.0746268656716, Blast_Score=187, Evalue=1e-48,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR016425 - InterPro: IPR004588 [H]
Pfam domain/function: PF04551 GcpE [H]
EC number: =1.17.7.1 [H]
Molecular weight: Translated: 46117; Mature: 46117
Theoretical pI: Translated: 5.71; Mature: 5.71
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.1 %Cys (Translated Protein) 2.8 %Met (Translated Protein) 4.9 %Cys+Met (Translated Protein) 2.1 %Cys (Mature Protein) 2.8 %Met (Mature Protein) 4.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MEISTPLDCAPALVGPLPRRNSRAAQIRWGDRVVHVGGDAPVCVQSMTNTDTSDAIGTAI CCCCCCCCCHHHHHCCCCCCCCCEEEEEECCEEEEECCCCCHHHHHHCCCCCHHHHHHHH QVKELARAGSELVRITVDTPAAAAAVPAIREQLDRMGVDVPLVGDFHYNGHKLLQDYPEC HHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH AQALSKYRINPGNVGQGAKRDTQFAQMIEIACRYDKPVRIGVNWGSLDQDLLARIMDENA HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCCCC QRATPWDARAVMVEALITSAIQSAQKAEELGLPGSQIILSCKVSAVQDLIAVYRELARRC CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHH HYALHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVIVAQEIL HHHHEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHH QTMGLRNFTPMVIACPGCGRTTSTVFQELAARIQSYLREQMPVWKEQYPGVEEMNVAVMG HHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHCCEEEEE CIVNGPGESKHANIGISLPGTGESPAAPVFVDGVKVKTLRGERITEEFQAIVDEYVRTHY EEECCCCCCCCCEEEEECCCCCCCCCCCEEECCEEEEEECCHHHHHHHHHHHHHHHHHHC GAQTPATV CCCCCCCC >Mature Secondary Structure MEISTPLDCAPALVGPLPRRNSRAAQIRWGDRVVHVGGDAPVCVQSMTNTDTSDAIGTAI CCCCCCCCCHHHHHCCCCCCCCCEEEEEECCEEEEECCCCCHHHHHHCCCCCHHHHHHHH QVKELARAGSELVRITVDTPAAAAAVPAIREQLDRMGVDVPLVGDFHYNGHKLLQDYPEC HHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH AQALSKYRINPGNVGQGAKRDTQFAQMIEIACRYDKPVRIGVNWGSLDQDLLARIMDENA HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCCCC QRATPWDARAVMVEALITSAIQSAQKAEELGLPGSQIILSCKVSAVQDLIAVYRELARRC CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHH HYALHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVIVAQEIL HHHHEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHH QTMGLRNFTPMVIACPGCGRTTSTVFQELAARIQSYLREQMPVWKEQYPGVEEMNVAVMG HHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHCCEEEEE CIVNGPGESKHANIGISLPGTGESPAAPVFVDGVKVKTLRGERITEEFQAIVDEYVRTHY EEECCCCCCCCCEEEEECCCCCCCCCCCEEECCEEEEEECCHHHHHHHHHHHHHHHHHHC GAQTPATV CCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11823852 [H]