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The map label for this gene is gltX

Identifier: 187928115

GI number: 187928115

Start: 1081484

End: 1082881

Strand: Direct

Name: gltX

Synonym: Rpic_1022

Alternate gene names: 187928115

Gene position: 1081484-1082881 (Clockwise)

Preceding gene: 187928113

Following gene: 187928122

Centisome position: 27.43

GC content: 63.88

Gene sequence:

>1398_bases
ATGACACAGCGCGTCCGTACTCGTTTCGCTCCCAGCCCCACCGGCTTCATCCACCTCGGCAACATCCGCTCGGCACTCTA
TCCGTGGGCCTTCGCACGCAAGATGAAGGGAGACTTCATCCTGCGCATCGAAGACACCGACGTGGAGCGCTCCAGCCAGG
AAGCCGTCGACGTGATCCTGGAAGCGATGGATTGGCTCGACATGGACATCGACGAAGGCCCGTTCTACCAGATGCAGCGC
ATGGACCGGTACCGCGCCGTGGTTGCTCAGATGGTGCAGCAGGAACTCGCCTACCCGTGCTACATGAGCACCGAAGAACT
CGACGCCCTGCGCGAAGCGCAACGCGAGCGCGGCGAGAAGCCCCGCTACGACGGCACCTGGCGCCCCGCACCCGGCAAGA
TTCTGCCGCCGCCGCCGGCCGGCGTGCAGCCAGTGATTCGCTTCAAGAACCCCATCGGCGGGAGTGTCGTGTGGGACGAC
GCCGTGAAGGGCCGCATCGAAATCTCCAACGATGAACTCGACGATCTCGTGATCGCGCGCCCGGACGGCACGCCTACGTA
TAACTTCTGTGTGGTCGTCGACGACATGGATATGCAGATCACGCATGTGATCCGCGGCGACGACCACGTCAACAACACGC
CCCGGCAGATCAACATCCTGCGCGCGCTGGGCGGTACGCCGCCGGTCTACGCACACCTGCCGACTGTGCTAAACGAGCAG
GGCGAGAAGATGAGCAAGCGCCACGGTGCCATGGCTGTCACGGGCTATCGTGATGCGGGCTATCTGCCAGAAGCCATCGT
CAACTACCTCGCCCGTCTGGGCTGGGCGCATGGCGACGCCGAGATCTTTTCGCGCGAGCAGTTCATCGAGTGGTTCGACC
TCGAGCACCTGGGCAAGTCGCCGGCGCAATACAACCCTGAGAAGCTTGCCTGGTTGAACAACCACTACATCAAGCAGGCT
GACAATGTGCGCCTGGCGGGGCTGGTGAAACCGTTCATCGAGGCGCTGGGTGGCAAGGTGGATGGCGGCCCTGTGCTGGC
TGACGTGGTTGCGCTCGTGAAGGATCGCGCCAACACGCTGCGTGAAGTCGCCCAGACAGCGCTGCTGTTCTATCGCTCGG
ATCTGGCTGTCGAGCCGGAGCTCGCTGCGCAGCACCTGACCGACGACGTGCGTCCTGGCATTGCGGCGCTGGCTGAGAAG
CTCGGGGCGCTGCCCGAGTGGAAGCGCGAGGCCATCGGCGCAGTCTTCAAGGAAGTTCTGGCGGCCCACGGCTGGAAGAT
GCCCAAGCTGGCGATGCCGGTGCGCCTGCTGGTGGCAGGGCAACTGCAAACGCCGTCGATTGATGCGGTGCTGGAGTTGT
TCGGTCGCGACGTCGTTCTGCATCGTCTGGCGGCGTGA

Upstream 100 bases:

>100_bases
ACACGTGCAGGACGGTTGCTATAATTTCAGTTTTGATAGAGGCCGCTCAGGATCCGCGCTCAGGGTTCGATCACGCGGCC
TTATTCGTTCAGCCATTCCT

Downstream 100 bases:

>100_bases
CGTGTGCAGCGCAAAAATTTTTGCGAAAGGGTATTGCCAAGCGCCAAATACTTCCCCTATACTCTTGTTTTCGCTGCAAC
ACGAAGCGAAATGCAGTAAG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MTQRVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGDFILRIEDTDVERSSQEAVDVILEAMDWLDMDIDEGPFYQMQR
MDRYRAVVAQMVQQELAYPCYMSTEELDALREAQRERGEKPRYDGTWRPAPGKILPPPPAGVQPVIRFKNPIGGSVVWDD
AVKGRIEISNDELDDLVIARPDGTPTYNFCVVVDDMDMQITHVIRGDDHVNNTPRQINILRALGGTPPVYAHLPTVLNEQ
GEKMSKRHGAMAVTGYRDAGYLPEAIVNYLARLGWAHGDAEIFSREQFIEWFDLEHLGKSPAQYNPEKLAWLNNHYIKQA
DNVRLAGLVKPFIEALGGKVDGGPVLADVVALVKDRANTLREVAQTALLFYRSDLAVEPELAAQHLTDDVRPGIAALAEK
LGALPEWKREAIGAVFKEVLAAHGWKMPKLAMPVRLLVAGQLQTPSIDAVLELFGRDVVLHRLAA

Sequences:

>Translated_465_residues
MTQRVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGDFILRIEDTDVERSSQEAVDVILEAMDWLDMDIDEGPFYQMQR
MDRYRAVVAQMVQQELAYPCYMSTEELDALREAQRERGEKPRYDGTWRPAPGKILPPPPAGVQPVIRFKNPIGGSVVWDD
AVKGRIEISNDELDDLVIARPDGTPTYNFCVVVDDMDMQITHVIRGDDHVNNTPRQINILRALGGTPPVYAHLPTVLNEQ
GEKMSKRHGAMAVTGYRDAGYLPEAIVNYLARLGWAHGDAEIFSREQFIEWFDLEHLGKSPAQYNPEKLAWLNNHYIKQA
DNVRLAGLVKPFIEALGGKVDGGPVLADVVALVKDRANTLREVAQTALLFYRSDLAVEPELAAQHLTDDVRPGIAALAEK
LGALPEWKREAIGAVFKEVLAAHGWKMPKLAMPVRLLVAGQLQTPSIDAVLELFGRDVVLHRLAA
>Mature_464_residues
TQRVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGDFILRIEDTDVERSSQEAVDVILEAMDWLDMDIDEGPFYQMQRM
DRYRAVVAQMVQQELAYPCYMSTEELDALREAQRERGEKPRYDGTWRPAPGKILPPPPAGVQPVIRFKNPIGGSVVWDDA
VKGRIEISNDELDDLVIARPDGTPTYNFCVVVDDMDMQITHVIRGDDHVNNTPRQINILRALGGTPPVYAHLPTVLNEQG
EKMSKRHGAMAVTGYRDAGYLPEAIVNYLARLGWAHGDAEIFSREQFIEWFDLEHLGKSPAQYNPEKLAWLNNHYIKQAD
NVRLAGLVKPFIEALGGKVDGGPVLADVVALVKDRANTLREVAQTALLFYRSDLAVEPELAAQHLTDDVRPGIAALAEKL
GALPEWKREAIGAVFKEVLAAHGWKMPKLAMPVRLLVAGQLQTPSIDAVLELFGRDVVLHRLAA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=489, Percent_Identity=30.0613496932515, Blast_Score=194, Evalue=1e-49,
Organism=Escherichia coli, GI1788743, Length=460, Percent_Identity=48.4782608695652, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI226510925, Length=248, Percent_Identity=33.4677419354839, Blast_Score=125, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI71993394, Length=472, Percent_Identity=31.3559322033898, Blast_Score=201, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6324540, Length=475, Percent_Identity=29.8947368421053, Blast_Score=210, Evalue=5e-55,
Organism=Drosophila melanogaster, GI24665321, Length=491, Percent_Identity=30.7535641547862, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_RALPJ (B2U9R2)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001898602.1
- GeneID:   6289456
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_1022
- HOGENOM:   HBG628189
- OMA:   DAQAREW
- ProtClustDB:   PRK01406
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52040; Mature: 51909

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS00213 LIPOCALIN

Important sites: BINDING 246-246

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQRVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGDFILRIEDTDVERSSQEAVDVIL
CCCCHHHCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
EAMDWLDMDIDEGPFYQMQRMDRYRAVVAQMVQQELAYPCYMSTEELDALREAQRERGEK
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC
PRYDGTWRPAPGKILPPPPAGVQPVIRFKNPIGGSVVWDDAVKGRIEISNDELDDLVIAR
CCCCCCCCCCCCCCCCCCCCCCCHHHEECCCCCCCEEECCCCCCEEEECCCCCCCEEEEC
PDGTPTYNFCVVVDDMDMQITHVIRGDDHVNNTPRQINILRALGGTPPVYAHLPTVLNEQ
CCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
GEKMSKRHGAMAVTGYRDAGYLPEAIVNYLARLGWAHGDAEIFSREQFIEWFDLEHLGKS
HHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHCCC
PAQYNPEKLAWLNNHYIKQADNVRLAGLVKPFIEALGGKVDGGPVLADVVALVKDRANTL
CCCCCCHHHHHHCCHHHHCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
REVAQTALLFYRSDLAVEPELAAQHLTDDVRPGIAALAEKLGALPEWKREAIGAVFKEVL
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
AAHGWKMPKLAMPVRLLVAGQLQTPSIDAVLELFGRDVVLHRLAA
HHCCCCCCHHHCCHHHEEECCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQRVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGDFILRIEDTDVERSSQEAVDVIL
CCCHHHCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
EAMDWLDMDIDEGPFYQMQRMDRYRAVVAQMVQQELAYPCYMSTEELDALREAQRERGEK
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC
PRYDGTWRPAPGKILPPPPAGVQPVIRFKNPIGGSVVWDDAVKGRIEISNDELDDLVIAR
CCCCCCCCCCCCCCCCCCCCCCCHHHEECCCCCCCEEECCCCCCEEEECCCCCCCEEEEC
PDGTPTYNFCVVVDDMDMQITHVIRGDDHVNNTPRQINILRALGGTPPVYAHLPTVLNEQ
CCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
GEKMSKRHGAMAVTGYRDAGYLPEAIVNYLARLGWAHGDAEIFSREQFIEWFDLEHLGKS
HHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHCCC
PAQYNPEKLAWLNNHYIKQADNVRLAGLVKPFIEALGGKVDGGPVLADVVALVKDRANTL
CCCCCCHHHHHHCCHHHHCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
REVAQTALLFYRSDLAVEPELAAQHLTDDVRPGIAALAEKLGALPEWKREAIGAVFKEVL
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
AAHGWKMPKLAMPVRLLVAGQLQTPSIDAVLELFGRDVVLHRLAA
HHCCCCCCHHHCCHHHEEECCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA