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The map label for this gene is phoB [H]

Identifier: 187928034

GI number: 187928034

Start: 993930

End: 994643

Strand: Direct

Name: phoB [H]

Synonym: Rpic_0939

Alternate gene names: 187928034

Gene position: 993930-994643 (Clockwise)

Preceding gene: 187928033

Following gene: 187928035

Centisome position: 25.21

GC content: 63.45

Gene sequence:

>714_bases
ATGGCGATACGAATTGCCTCGGTGGAAGACAACCAGGCGCAATCGGATTTGATCCGCGAGATCCTGATTTCGTCTGGATA
CGAATGCGAGTGTTTCAGTAACGGGGATGCCTTCGTCAAGGCGCTGCGGGATCGCACGTTCGACCTGCTGCTGCTCGACT
GGCAGTTGCCCGATATCAGCGGCATCCAGCTTGTGAGCTGGGTGCGGCAGACGGTCGGCCCGGCGCTGCCGGTGCTGTTT
CTGACCAACCGTTCGCTCGAAGACGACGTGGTGCGTGGGCTGGCTGCCGGCGCCGACGATTACGTCGTCAAGCCGGTGCG
CCGCGCCGAGCTGGTGGCGCGCGTCGGTGCGCTGCTGCGTCGTGCCGCGCCGCGCACGAGCGAATCGCTCGAACGCATTC
GCGTCGGCCCGTACGCAGTGGACCCCGTCGGGCGCGTGCTGACGTTGAATGGCTCGCAGGTTGAGCTTTCGCCGCGCGAG
TTCGATCTTGGGCTGTACCTGTTCCGCAATGTCGGCAAGCTGGTGCCGCGTGAGCTGATCGAGCAGGCAGTGTGGGGGCG
CAGCATCGGCCCGGATTCGCGCACGCTTGCCACCCATATCTCCAAGCTGCGGCTCAAACTCGATCTCAATCAGAAGAATG
GCGTGCGGCTGGTCTCGGTGTACTCGCACGGCTATCGGCTCGAAACCACGCAGGAAGATGGTGAAGCAAGCTGA

Upstream 100 bases:

>100_bases
TACGATGCGCCGGTCCGGGACCCCGATCACAACGACGCACGGAGTGAGCCGGCATTTCGCATAGCGGTGCCGGACCCGAA
ACGGGGAAAGGGCAATCCAG

Downstream 100 bases:

>100_bases
CGCAATCTGGGGTGGTTTGATTGGGCGCGCCGCGTTTGTCGGCGCGTTCGTTCTTTTTGGCGGCGTGTGGCAGGCGGCGA
TGGCTCAGCCTTCCGGCGCT

Product: winged helix family two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MAIRIASVEDNQAQSDLIREILISSGYECECFSNGDAFVKALRDRTFDLLLLDWQLPDISGIQLVSWVRQTVGPALPVLF
LTNRSLEDDVVRGLAAGADDYVVKPVRRAELVARVGALLRRAAPRTSESLERIRVGPYAVDPVGRVLTLNGSQVELSPRE
FDLGLYLFRNVGKLVPRELIEQAVWGRSIGPDSRTLATHISKLRLKLDLNQKNGVRLVSVYSHGYRLETTQEDGEAS

Sequences:

>Translated_237_residues
MAIRIASVEDNQAQSDLIREILISSGYECECFSNGDAFVKALRDRTFDLLLLDWQLPDISGIQLVSWVRQTVGPALPVLF
LTNRSLEDDVVRGLAAGADDYVVKPVRRAELVARVGALLRRAAPRTSESLERIRVGPYAVDPVGRVLTLNGSQVELSPRE
FDLGLYLFRNVGKLVPRELIEQAVWGRSIGPDSRTLATHISKLRLKLDLNQKNGVRLVSVYSHGYRLETTQEDGEAS
>Mature_236_residues
AIRIASVEDNQAQSDLIREILISSGYECECFSNGDAFVKALRDRTFDLLLLDWQLPDISGIQLVSWVRQTVGPALPVLFL
TNRSLEDDVVRGLAAGADDYVVKPVRRAELVARVGALLRRAAPRTSESLERIRVGPYAVDPVGRVLTLNGSQVELSPREF
DLGLYLFRNVGKLVPRELIEQAVWGRSIGPDSRTLATHISKLRLKLDLNQKNGVRLVSVYSHGYRLETTQEDGEAS

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=231, Percent_Identity=35.0649350649351, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1786784, Length=226, Percent_Identity=33.1858407079646, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1790860, Length=202, Percent_Identity=37.6237623762376, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1789402, Length=205, Percent_Identity=35.1219512195122, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI87082012, Length=207, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1790552, Length=210, Percent_Identity=31.9047619047619, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786911, Length=224, Percent_Identity=35.2678571428571, Blast_Score=97, Evalue=6e-22,
Organism=Escherichia coli, GI1788394, Length=225, Percent_Identity=32, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787229, Length=236, Percent_Identity=30.5084745762712, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790863, Length=238, Percent_Identity=28.9915966386555, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI2367329, Length=209, Percent_Identity=34.9282296650718, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787375, Length=227, Percent_Identity=29.5154185022026, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1789809, Length=228, Percent_Identity=25.8771929824561, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI145693140, Length=230, Percent_Identity=27.8260869565217, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1790299, Length=108, Percent_Identity=34.2592592592593, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26354; Mature: 26223

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRIASVEDNQAQSDLIREILISSGYECECFSNGDAFVKALRDRTFDLLLLDWQLPDIS
CEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCEEEEEEEECCCCCC
GIQLVSWVRQTVGPALPVLFLTNRSLEDDVVRGLAAGADDYVVKPVRRAELVARVGALLR
HHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHCHHHHHHHHHHHHHHHH
RAAPRTSESLERIRVGPYAVDPVGRVLTLNGSQVELSPREFDLGLYLFRNVGKLVPRELI
HCCCCCHHHHHHHCCCCEEECCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
EQAVWGRSIGPDSRTLATHISKLRLKLDLNQKNGVRLVSVYSHGYRLETTQEDGEAS
HHHHCCCCCCCCCHHHHHHHHHHEEEEECCCCCCEEEEEEECCCEEEEECCCCCCCC
>Mature Secondary Structure 
AIRIASVEDNQAQSDLIREILISSGYECECFSNGDAFVKALRDRTFDLLLLDWQLPDIS
EEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCEEEEEEEECCCCCC
GIQLVSWVRQTVGPALPVLFLTNRSLEDDVVRGLAAGADDYVVKPVRRAELVARVGALLR
HHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHCHHHHHHHHHHHHHHHH
RAAPRTSESLERIRVGPYAVDPVGRVLTLNGSQVELSPREFDLGLYLFRNVGKLVPRELI
HCCCCCHHHHHHHCCCCEEECCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
EQAVWGRSIGPDSRTLATHISKLRLKLDLNQKNGVRLVSVYSHGYRLETTQEDGEAS
HHHHCCCCCCCCCHHHHHHHHHHEEEEECCCCCCEEEEEEECCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]