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The map label for this gene is iscA [H]

Identifier: 187927981

GI number: 187927981

Start: 940473

End: 940793

Strand: Direct

Name: iscA [H]

Synonym: Rpic_0886

Alternate gene names: 187927981

Gene position: 940473-940793 (Clockwise)

Preceding gene: 187927980

Following gene: 187927982

Centisome position: 23.85

GC content: 60.75

Gene sequence:

>321_bases
ATGATCACCCTGACCGACAAGGCCGCGCAGCACGTTTCCCGCTACCTGACCCGCCGTGGCAAGGGCGTGGGCCTGCGCGT
GGGCGTGAAGACCACGGGCTGTTCGGGCCTGGCCTACAAGCTCGAATATGCAGATGAGATTGCCACCGAAGACCAGGTCT
TCGAATCCAACGGCGTCAAGGTGATCGTCGATCCGAAGAGCCTGCCGTACATCGACGGCACTGAGTTGGACTACGCACGC
GAGGGGCTGAACGAAGGGTTCCGCTTCAATAATCCAAACGTGAAGGACGAGTGCGGCTGCGGTGAATCGTTCCGCGTGTG
A

Upstream 100 bases:

>100_bases
CCGAAGACGCCATCAAGGCGGCCGTGGCCGACTACAAGGAAAAGCACGGTTCGGCCGAGCAGAAGGCTGCTTGAGCCGGT
TTGGATAGAAAGGACGCAGC

Downstream 100 bases:

>100_bases
GCGGGGCACATTGAAGCAGCCGATTGTCGGTTTCGAGCGGGACGAGGAGGGCCATTGGGTCGCCCGTCTCGCTTGCGGAC
ATGGGCAGCATATGCGCCAC

Product: iron-sulfur cluster assembly protein IscA

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 106; Mature: 106

Protein sequence:

>106_residues
MITLTDKAAQHVSRYLTRRGKGVGLRVGVKTTGCSGLAYKLEYADEIATEDQVFESNGVKVIVDPKSLPYIDGTELDYAR
EGLNEGFRFNNPNVKDECGCGESFRV

Sequences:

>Translated_106_residues
MITLTDKAAQHVSRYLTRRGKGVGLRVGVKTTGCSGLAYKLEYADEIATEDQVFESNGVKVIVDPKSLPYIDGTELDYAR
EGLNEGFRFNNPNVKDECGCGESFRV
>Mature_106_residues
MITLTDKAAQHVSRYLTRRGKGVGLRVGVKTTGCSGLAYKLEYADEIATEDQVFESNGVKVIVDPKSLPYIDGTELDYAR
EGLNEGFRFNNPNVKDECGCGESFRV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=105, Percent_Identity=41.9047619047619, Blast_Score=95, Evalue=1e-20,
Organism=Homo sapiens, GI160420328, Length=107, Percent_Identity=36.4485981308411, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI1788877, Length=105, Percent_Identity=66.6666666666667, Blast_Score=152, Evalue=3e-39,
Organism=Escherichia coli, GI1787974, Length=105, Percent_Identity=47.6190476190476, Blast_Score=114, Evalue=1e-27,
Organism=Escherichia coli, GI1786351, Length=108, Percent_Identity=40.7407407407407, Blast_Score=75, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25146411, Length=105, Percent_Identity=40, Blast_Score=95, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI193209025, Length=105, Percent_Identity=40, Blast_Score=95, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71998452, Length=106, Percent_Identity=33.9622641509434, Blast_Score=63, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323001, Length=106, Percent_Identity=44.3396226415094, Blast_Score=94, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24642263, Length=105, Percent_Identity=42.8571428571429, Blast_Score=93, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24649697, Length=86, Percent_Identity=38.3720930232558, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 11680; Mature: 11680

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLTDKAAQHVSRYLTRRGKGVGLRVGVKTTGCSGLAYKLEYADEIATEDQVFESNGVK
CEEECHHHHHHHHHHHHHCCCCCEEEEECEECCCCCEEEEEECHHHHCCCCCEECCCCCE
VIVDPKSLPYIDGTELDYAREGLNEGFRFNNPNVKDECGCGESFRV
EEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MITLTDKAAQHVSRYLTRRGKGVGLRVGVKTTGCSGLAYKLEYADEIATEDQVFESNGVK
CEEECHHHHHHHHHHHHHCCCCCEEEEECEECCCCCEEEEEECHHHHCCCCCEECCCCCE
VIVDPKSLPYIDGTELDYAREGLNEGFRFNNPNVKDECGCGESFRV
EEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]