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The map label for this gene is alaXM [H]

Identifier: 187927944

GI number: 187927944

Start: 901249

End: 901968

Strand: Reverse

Name: alaXM [H]

Synonym: Rpic_0849

Alternate gene names: 187927944

Gene position: 901968-901249 (Counterclockwise)

Preceding gene: 187927948

Following gene: 187927943

Centisome position: 22.88

GC content: 66.53

Gene sequence:

>720_bases
ATGCCAACGTTGAAACGTTTTGACGAAGACGCCTACCGCGCCACCTGCGACGCCACCGTCGTCGCCGTGCATTCGGAAGG
CATTGAGCTGGACGCCACGGTGTTCTACGCACGCAGCGGCGGCCAGGCTGGCGACACCGGCACACTCGTCCTGGCCGATG
GAACAACCATCAGGATTGCCGACACCGTCTACAGCCCGGATCGCCAAACCGTCCTGCATGTGCCCGCCGACAATGCTGGC
CTCACGCTGCTCGCGCCCGGCACGGCCGTCACGGCCACCATCGACTGGGACCGCCGCCACCGGCTGATGCGGCTGCATAC
GTGCCTGCACCTGCTGGGCTCGCTGATTCCGGTGCCCGTCACGGGCTGCGGTATCTCGCCAGACGCCGGCCGCATCGATT
TCGACCTGCCGGAATCAACGCTCGACCGCGACACGCTGACCACACAGCTCAACGAACTCATCGGCCGTAACACGCCGGTG
CGCATCGACCTGATCACCCCGGAAGAACTGGCCGCCCAGCCCGAACTCGTGCGCACCATCGGCGCGGCGCCGCCGGCGGG
CGCATCGGCCATCCGCATCATCCACATCGACGGCATCGACCGGCAGCCGTGCGGTGGCACCCACGTGCGCGCCACCGGCG
AAATCGGCCGCGTGATCGTCACCAAGATCGAAAAAAAGAGCCGCCAGAACCGACGCGTGGCCGTGGCGTTTGCAGATTAA

Upstream 100 bases:

>100_bases
GTACAGAAAGCGCCACGACAAAGTGTACAGGTACTGTACAGACGCAGTTCGGTAGGATCAAGGTCAGAATTCCGCCCCCA
GCGAACCCGGAGACGCCCCC

Downstream 100 bases:

>100_bases
GTGGCCACCATGAACAAGTCCGCCGCCCTCCCCATGCAACACGCCGACCACGCCGAACGCCGGGGCTTGCTGCTCGGCCT
CATCGGCGTGGCCATCTTTA

Product: Threonyl/alanyl tRNA synthetase SAD

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-M; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MPTLKRFDEDAYRATCDATVVAVHSEGIELDATVFYARSGGQAGDTGTLVLADGTTIRIADTVYSPDRQTVLHVPADNAG
LTLLAPGTAVTATIDWDRRHRLMRLHTCLHLLGSLIPVPVTGCGISPDAGRIDFDLPESTLDRDTLTTQLNELIGRNTPV
RIDLITPEELAAQPELVRTIGAAPPAGASAIRIIHIDGIDRQPCGGTHVRATGEIGRVIVTKIEKKSRQNRRVAVAFAD

Sequences:

>Translated_239_residues
MPTLKRFDEDAYRATCDATVVAVHSEGIELDATVFYARSGGQAGDTGTLVLADGTTIRIADTVYSPDRQTVLHVPADNAG
LTLLAPGTAVTATIDWDRRHRLMRLHTCLHLLGSLIPVPVTGCGISPDAGRIDFDLPESTLDRDTLTTQLNELIGRNTPV
RIDLITPEELAAQPELVRTIGAAPPAGASAIRIIHIDGIDRQPCGGTHVRATGEIGRVIVTKIEKKSRQNRRVAVAFAD
>Mature_238_residues
PTLKRFDEDAYRATCDATVVAVHSEGIELDATVFYARSGGQAGDTGTLVLADGTTIRIADTVYSPDRQTVLHVPADNAGL
TLLAPGTAVTATIDWDRRHRLMRLHTCLHLLGSLIPVPVTGCGISPDAGRIDFDLPESTLDRDTLTTQLNELIGRNTPVR
IDLITPEELAAQPELVRTIGAAPPAGASAIRIIHIDGIDRQPCGGTHVRATGEIGRVIVTKIEKKSRQNRRVAVAFAD

Specific function: Functions in trans to edit the amino acid moiety from incorrectly charged Ser-tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Homo sapiens, GI217416402, Length=209, Percent_Identity=28.2296650717703, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI13376886, Length=209, Percent_Identity=28.2296650717703, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1789048, Length=218, Percent_Identity=29.3577981651376, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 25626; Mature: 25495

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTLKRFDEDAYRATCDATVVAVHSEGIELDATVFYARSGGQAGDTGTLVLADGTTIRIA
CCCHHHCCCHHHEEECCEEEEEEECCCCEEEEEEEEEECCCCCCCCEEEEEECCCEEEEE
DTVYSPDRQTVLHVPADNAGLTLLAPGTAVTATIDWDRRHRLMRLHTCLHLLGSLIPVPV
EEEECCCCCEEEEECCCCCCEEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCE
TGCGISPDAGRIDFDLPESTLDRDTLTTQLNELIGRNTPVRIDLITPEELAAQPELVRTI
ECCCCCCCCCEEEECCCHHHCCHHHHHHHHHHHHCCCCCEEEEEECHHHHHCCHHHHHHH
GAAPPAGASAIRIIHIDGIDRQPCGGTHVRATGEIGRVIVTKIEKKSRQNRRVAVAFAD
CCCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCEEEEEEEHHHCCCCCEEEEEEEC
>Mature Secondary Structure 
PTLKRFDEDAYRATCDATVVAVHSEGIELDATVFYARSGGQAGDTGTLVLADGTTIRIA
CCHHHCCCHHHEEECCEEEEEEECCCCEEEEEEEEEECCCCCCCCEEEEEECCCEEEEE
DTVYSPDRQTVLHVPADNAGLTLLAPGTAVTATIDWDRRHRLMRLHTCLHLLGSLIPVPV
EEEECCCCCEEEEECCCCCCEEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCE
TGCGISPDAGRIDFDLPESTLDRDTLTTQLNELIGRNTPVRIDLITPEELAAQPELVRTI
ECCCCCCCCCEEEECCCHHHCCHHHHHHHHHHHHCCCCCEEEEEECHHHHHCCHHHHHHH
GAAPPAGASAIRIIHIDGIDRQPCGGTHVRATGEIGRVIVTKIEKKSRQNRRVAVAFAD
CCCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCEEEEEEEHHHCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]