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The map label for this gene is citH [H]

Identifier: 187927930

GI number: 187927930

Start: 882201

End: 883520

Strand: Direct

Name: citH [H]

Synonym: Rpic_0835

Alternate gene names: 187927930

Gene position: 882201-883520 (Clockwise)

Preceding gene: 187927929

Following gene: 187927933

Centisome position: 22.38

GC content: 66.44

Gene sequence:

>1320_bases
ATGTCGAACCAAACCTCTGCGCCGTCGTACGCCGGCCGCGCCGATGCGCAGGCGACAGGCCACGCGCAACCGCTGTCACG
CACGCGCGCCATTGCCGCCATCACCATCGGCAACGGCCTCGAGTTCTATGACTTCGTGGTCTATAGCTTCTTCGCCACGC
TGATCGGCCGGCTGTACTTTCCGGTCGGCAACGCGACCGGCCAGTTGCTGATGTCGTTCGCCACATTTGGCGTGGGTTTC
CTGATGCGGCCGCTCGGCGGTTTGCTGATCGGCATGTACGCCGATCGGGCGGGCCGCAAGCCGGCGGTGGCGCTCACGTT
GTGGCTGATGGGTCTGTCGTCGCTGATCTTCGTCGTCACGCCCACTTACGCCCAGATCGGCATCCTCGCGCCCATCCTCG
TCGTGCTTGCGCGGCTTGTGCAGGGCTTTGCCATCGGTGGCGAGATGGGTGCTTCGACTGCGCTGCTGCTGGAATACGCT
GATGATCGTTCGCGCGGCTTCTATACGAGCTGGCAACCGTTCAGCCAGGGGCTGGCGGCGCTGTTCGGCGCGCTGGTCGG
CCTGCTGCTGAGCAACGTGCTGGCGCCTGGGGCGCTGGAGTCGTGGGGCTGGCGGTTGGCGTTCGTGATCGGCATTCTCG
TCATCCCGGTCGGCCTGATCATCCGCCGCCGCCTGGAGGAAACCGCCGCACCGCCCAGCACGCGCGAAGCCGACGCCACG
CTGCCGTTGCTGCGCGAACACCGTCGCACGCTGGTGGCGAGCATCCTGCTGATGATCGGGGTGGCGTCGTCCACGTACAT
CATCGTCTACTACCTCAGCAACTACGCTGTGAGCGTGCTGCACATGCCGCTGTCGCTGGGCATCTGGGCGGCGTGCATCG
CTGCGCTGGTGCAGGTGGTGCTGTCGCCGTTCGCGGGGCGGCTGGCCGACCGCGTGGGGCGCCGCAGGGTGGTGCTGTGG
TCGCGCGTTGCACTGCTGGCGATGATCTATCCGGCCTTTGCGCTCATCAACGTAGACCCATCGCTCGCCCGGTTGCTGAT
CGTGGTCGGCTGCCTGTCGGTGCCGATGTCGATGACTTCGCCGGCCTCGATGGTGCTCGTGAGCGAGGTGCTGCCGCAGC
GGCTGCGCGCCACGGGGCTGTCGATTGCCTACTGCGTCGCCATCGCCATCTTTGGCGGCTTTGCGCAGTTCTTCTCGACC
GAGCTGATCCAGCTCACAGGCAATGCCAACGCACCGGCTTTCTACGTGATCGGCTGCGGCCTCGTCTCGCTGATCGGGCT
GGCCATGGTGCCCGAGACGCTCGGCCGCCGCCTGTCGTAA

Upstream 100 bases:

>100_bases
TGCGCGTGAAGCCCAACGCAGCAGCCGTTCTGGCTGAAGAGGCAAGCGGGGTGTGAGCCCCGCCCGCCCGCAAAGACAAG
ACAAGTTTCGGAGACACCCC

Downstream 100 bases:

>100_bases
CGCTCAATCGACCCCAACGAAACCGCCGGTCTGGTGCGCCCACAGCCGTGCATACAGGCCGCCGTGTTCCAGCAACTCCG
CATGCGTGCCGCTCTCGACG

Product: major facilitator superfamily protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MSNQTSAPSYAGRADAQATGHAQPLSRTRAIAAITIGNGLEFYDFVVYSFFATLIGRLYFPVGNATGQLLMSFATFGVGF
LMRPLGGLLIGMYADRAGRKPAVALTLWLMGLSSLIFVVTPTYAQIGILAPILVVLARLVQGFAIGGEMGASTALLLEYA
DDRSRGFYTSWQPFSQGLAALFGALVGLLLSNVLAPGALESWGWRLAFVIGILVIPVGLIIRRRLEETAAPPSTREADAT
LPLLREHRRTLVASILLMIGVASSTYIIVYYLSNYAVSVLHMPLSLGIWAACIAALVQVVLSPFAGRLADRVGRRRVVLW
SRVALLAMIYPAFALINVDPSLARLLIVVGCLSVPMSMTSPASMVLVSEVLPQRLRATGLSIAYCVAIAIFGGFAQFFST
ELIQLTGNANAPAFYVIGCGLVSLIGLAMVPETLGRRLS

Sequences:

>Translated_439_residues
MSNQTSAPSYAGRADAQATGHAQPLSRTRAIAAITIGNGLEFYDFVVYSFFATLIGRLYFPVGNATGQLLMSFATFGVGF
LMRPLGGLLIGMYADRAGRKPAVALTLWLMGLSSLIFVVTPTYAQIGILAPILVVLARLVQGFAIGGEMGASTALLLEYA
DDRSRGFYTSWQPFSQGLAALFGALVGLLLSNVLAPGALESWGWRLAFVIGILVIPVGLIIRRRLEETAAPPSTREADAT
LPLLREHRRTLVASILLMIGVASSTYIIVYYLSNYAVSVLHMPLSLGIWAACIAALVQVVLSPFAGRLADRVGRRRVVLW
SRVALLAMIYPAFALINVDPSLARLLIVVGCLSVPMSMTSPASMVLVSEVLPQRLRATGLSIAYCVAIAIFGGFAQFFST
ELIQLTGNANAPAFYVIGCGLVSLIGLAMVPETLGRRLS
>Mature_438_residues
SNQTSAPSYAGRADAQATGHAQPLSRTRAIAAITIGNGLEFYDFVVYSFFATLIGRLYFPVGNATGQLLMSFATFGVGFL
MRPLGGLLIGMYADRAGRKPAVALTLWLMGLSSLIFVVTPTYAQIGILAPILVVLARLVQGFAIGGEMGASTALLLEYAD
DRSRGFYTSWQPFSQGLAALFGALVGLLLSNVLAPGALESWGWRLAFVIGILVIPVGLIIRRRLEETAAPPSTREADATL
PLLREHRRTLVASILLMIGVASSTYIIVYYLSNYAVSVLHMPLSLGIWAACIAALVQVVLSPFAGRLADRVGRRRVVLWS
RVALLAMIYPAFALINVDPSLARLLIVVGCLSVPMSMTSPASMVLVSEVLPQRLRATGLSIAYCVAIAIFGGFAQFFSTE
LIQLTGNANAPAFYVIGCGLVSLIGLAMVPETLGRRLS

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=431, Percent_Identity=32.0185614849188, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1788942, Length=430, Percent_Identity=33.953488372093, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1788292, Length=416, Percent_Identity=29.8076923076923, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1789941, Length=445, Percent_Identity=30.561797752809, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI87082231, Length=251, Percent_Identity=27.0916334661355, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46851; Mature: 46720

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQTSAPSYAGRADAQATGHAQPLSRTRAIAAITIGNGLEFYDFVVYSFFATLIGRLYF
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHC
PVGNATGQLLMSFATFGVGFLMRPLGGLLIGMYADRAGRKPAVALTLWLMGLSSLIFVVT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
PTYAQIGILAPILVVLARLVQGFAIGGEMGASTALLLEYADDRSRGFYTSWQPFSQGLAA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
LFGALVGLLLSNVLAPGALESWGWRLAFVIGILVIPVGLIIRRRLEETAAPPSTREADAT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
LPLLREHRRTLVASILLMIGVASSTYIIVYYLSNYAVSVLHMPLSLGIWAACIAALVQVV
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSPFAGRLADRVGRRRVVLWSRVALLAMIYPAFALINVDPSLARLLIVVGCLSVPMSMTS
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHCCCCCCC
PASMVLVSEVLPQRLRATGLSIAYCVAIAIFGGFAQFFSTELIQLTGNANAPAFYVIGCG
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEHHHH
LVSLIGLAMVPETLGRRLS
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SNQTSAPSYAGRADAQATGHAQPLSRTRAIAAITIGNGLEFYDFVVYSFFATLIGRLYF
CCCCCCCCCCCCCCCCCCCCCCHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHC
PVGNATGQLLMSFATFGVGFLMRPLGGLLIGMYADRAGRKPAVALTLWLMGLSSLIFVVT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
PTYAQIGILAPILVVLARLVQGFAIGGEMGASTALLLEYADDRSRGFYTSWQPFSQGLAA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
LFGALVGLLLSNVLAPGALESWGWRLAFVIGILVIPVGLIIRRRLEETAAPPSTREADAT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
LPLLREHRRTLVASILLMIGVASSTYIIVYYLSNYAVSVLHMPLSLGIWAACIAALVQVV
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSPFAGRLADRVGRRRVVLWSRVALLAMIYPAFALINVDPSLARLLIVVGCLSVPMSMTS
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHCCCCCCC
PASMVLVSEVLPQRLRATGLSIAYCVAIAIFGGFAQFFSTELIQLTGNANAPAFYVIGCG
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEHHHH
LVSLIGLAMVPETLGRRLS
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]