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The map label for this gene is cobD [H]

Identifier: 187927904

GI number: 187927904

Start: 853615

End: 854556

Strand: Direct

Name: cobD [H]

Synonym: Rpic_0809

Alternate gene names: 187927904

Gene position: 853615-854556 (Clockwise)

Preceding gene: 187927903

Following gene: 187927908

Centisome position: 21.65

GC content: 62.1

Gene sequence:

>942_bases
ATGACTTTCTTCTCCGTACTCTGCGCGCTGATTCTCGAACAGTTCCGTGCGCTCAGCCGGGACAACCCGATCTACGACGT
CGTGCGCGCACTGGCTGCGCGGGCAGAAGAATGGTTCGACACCGGCCTTCGTCGCGATGCCGTCCTCGCTTGGTGCGTGG
TCACGCTGCCGGCCGTGGTGGTCGTGGCACTGGTGCACTACCTGCTGTCGTCGATCAGTCTTGGGCTGGCGTTCCTGTGG
AACGTCCTGATGGTTTACCTGACGCTCGGCTTCCGCCAGTTCAGTCACTACTTCACCGATATCCATGAGGCACTGCGCAG
CGATGACGTTGTCACTGCGCGCCTGCTGCTCAACGAATGGTCGGGGCAGGACACGACCGACATGCCGGTCAATGAGATCG
TCCGCCATACCCTGGAGTGCGCGATTGTTGCGGCGCATCGTCATGTGTTCGGTGTGTTCTTCTGGTTCCTGGTGCCGATC
GGGCCGGCGGGTGTGGTGCTATACCGTGGCGCTGAATACCTCGCTCGCCACTGGAGCGATACGTCGACGGAGCGTAGCCC
CGCGCTAGGTCTCTTTGCCCGTCAGGCGTTTTTCATCATCGACTACGTCCCTGCGCGTCTCACTGCGATCGGCTTTTCCA
TCGTCGGCAACTTCGAAGATGCCGTGTACGCCTGGCGCAACCGCGCTGCCAAGTGGAATGACGAAGTGAATGGAATCCTA
TTGGCGGCCGGCGGTGGCGCGCTCGGCGTGCGTCTTGGCACGCCCATTCCGCAACGCGATTCGGCCGATAGCATTGCCGA
CGAGGATGGCGCCTATCCCATGGAAGTGGGGCAGGAGCCGAACGTGCGTACCTTGCAGTCAGCTGTGGGGCTGGTGTGGC
GCGCCGTGGTGTTGTGGATGCTGCTGCTGGCGATGCTGTCGATTGCCGTGTGGCTCGGCTGA

Upstream 100 bases:

>100_bases
GCGAATATGGGCACCCTGTGCGCTGTTGCGCGCACGCTGTGTGCTATCGTCTTGCACATCGCATTCACGGTGTGATCCAC
GCCTTCGCAACGCCCTTCCA

Downstream 100 bases:

>100_bases
TGCGGCACGACGCTTAGTCGCCGAACGATCAATCACGCCGCATCCTCGCGGTTGTTGCCGCAGCGCCAGCACTGGGCGAA
CTGCGGCTCGTGACTCTCTC

Product: CobD/CbiB family protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MTFFSVLCALILEQFRALSRDNPIYDVVRALAARAEEWFDTGLRRDAVLAWCVVTLPAVVVVALVHYLLSSISLGLAFLW
NVLMVYLTLGFRQFSHYFTDIHEALRSDDVVTARLLLNEWSGQDTTDMPVNEIVRHTLECAIVAAHRHVFGVFFWFLVPI
GPAGVVLYRGAEYLARHWSDTSTERSPALGLFARQAFFIIDYVPARLTAIGFSIVGNFEDAVYAWRNRAAKWNDEVNGIL
LAAGGGALGVRLGTPIPQRDSADSIADEDGAYPMEVGQEPNVRTLQSAVGLVWRAVVLWMLLLAMLSIAVWLG

Sequences:

>Translated_313_residues
MTFFSVLCALILEQFRALSRDNPIYDVVRALAARAEEWFDTGLRRDAVLAWCVVTLPAVVVVALVHYLLSSISLGLAFLW
NVLMVYLTLGFRQFSHYFTDIHEALRSDDVVTARLLLNEWSGQDTTDMPVNEIVRHTLECAIVAAHRHVFGVFFWFLVPI
GPAGVVLYRGAEYLARHWSDTSTERSPALGLFARQAFFIIDYVPARLTAIGFSIVGNFEDAVYAWRNRAAKWNDEVNGIL
LAAGGGALGVRLGTPIPQRDSADSIADEDGAYPMEVGQEPNVRTLQSAVGLVWRAVVLWMLLLAMLSIAVWLG
>Mature_312_residues
TFFSVLCALILEQFRALSRDNPIYDVVRALAARAEEWFDTGLRRDAVLAWCVVTLPAVVVVALVHYLLSSISLGLAFLWN
VLMVYLTLGFRQFSHYFTDIHEALRSDDVVTARLLLNEWSGQDTTDMPVNEIVRHTLECAIVAAHRHVFGVFFWFLVPIG
PAGVVLYRGAEYLARHWSDTSTERSPALGLFARQAFFIIDYVPARLTAIGFSIVGNFEDAVYAWRNRAAKWNDEVNGILL
AAGGGALGVRLGTPIPQRDSADSIADEDGAYPMEVGQEPNVRTLQSAVGLVWRAVVLWMLLLAMLSIAVWLG

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 34816; Mature: 34684

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00496 PII_GLNB_UMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFFSVLCALILEQFRALSRDNPIYDVVRALAARAEEWFDTGLRRDAVLAWCVVTLPAVV
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VVALVHYLLSSISLGLAFLWNVLMVYLTLGFRQFSHYFTDIHEALRSDDVVTARLLLNEW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
SGQDTTDMPVNEIVRHTLECAIVAAHRHVFGVFFWFLVPIGPAGVVLYRGAEYLARHWSD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHCCC
TSTERSPALGLFARQAFFIIDYVPARLTAIGFSIVGNFEDAVYAWRNRAAKWNDEVNGIL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCEEE
LAAGGGALGVRLGTPIPQRDSADSIADEDGAYPMEVGQEPNVRTLQSAVGLVWRAVVLWM
EEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LLLAMLSIAVWLG
HHHHHHHHHHHCC
>Mature Secondary Structure 
TFFSVLCALILEQFRALSRDNPIYDVVRALAARAEEWFDTGLRRDAVLAWCVVTLPAVV
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VVALVHYLLSSISLGLAFLWNVLMVYLTLGFRQFSHYFTDIHEALRSDDVVTARLLLNEW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
SGQDTTDMPVNEIVRHTLECAIVAAHRHVFGVFFWFLVPIGPAGVVLYRGAEYLARHWSD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHCCC
TSTERSPALGLFARQAFFIIDYVPARLTAIGFSIVGNFEDAVYAWRNRAAKWNDEVNGIL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCEEE
LAAGGGALGVRLGTPIPQRDSADSIADEDGAYPMEVGQEPNVRTLQSAVGLVWRAVVLWM
EEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LLLAMLSIAVWLG
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA