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The map label for this gene is exoA [H]

Identifier: 187927152

GI number: 187927152

Start: 53778

End: 54563

Strand: Direct

Name: exoA [H]

Synonym: Rpic_0042

Alternate gene names: 187927152

Gene position: 53778-54563 (Clockwise)

Preceding gene: 187927150

Following gene: 187927153

Centisome position: 1.36

GC content: 61.58

Gene sequence:

>786_bases
ATGTTACGCATCATTTCCGCCAACCTCAACGGCGTTCGCTCCGCTGCCAGCAAAGGCTTTTTTGATTGGATGGGCAAGCA
GGACGCCGATTTCGTCTGCGTGCAGGAACTGAAATGCGCGCAAGACGACATGACCCCGGAATTTCTGGCGCCGCACGGCT
ACCACGGCGTGTTCCAGCACGCGGTAAAGAAAGGCTACAGCGGCGCGGGCCTGTACACGCGCCACAAGCCCGACGAAGTC
ATCGTCGGCTTCGATAACGGCGAGTTCGATGCCGAAGGCCGCTACGTCGAGGCCCGCTACGGCAAGCTGTCGGTCATCTC
GGTCTACGTGCCGTCGGGCTCCAGCGGCGAAGAGCGTCAGCAGGCCAAGTTCCGCTTCATGGACCTGTTCATGGAGCACC
TCAAGGACCTGCGCCACGAAAAGGGCCGCGAGGTCGTGCTGTGCGGCGACGTCAACATCGTCCACAAGGAAATCGACATC
AAGAACTGGAAGGGCAACCAGAAGAACTCCGGCTGCCTGCCCGAAGAGCGCGCGTGGCTGACCAAGCTCTTCGATGAGGT
CGGCTACGTGGACGTGTTCCGCACGCTCGACCAGCGCCCCGAGCAGTACACGTGGTGGAGCAACCGCGGCCAGGCATACG
CAAAGAACGTCGGGTGGCGTATCGATTACCAGATCGCCACGCCGGGCATTGCCGCCACGGCACGCCGCGCGTCGATCTTC
AAGGACATCAAGTTCAGCGACCACGCGCCGCTGACGATCGATTACGACACGAAGCCGCACTGGTAA

Upstream 100 bases:

>100_bases
ATGACAGCACGCCGGCCTCCACCGCAAAGCGGATGAAGGATTGGCGCAACTCGCTGTTTTGGCTCATCTTTTTTTCTCTG
GTCCGCAAAAAAGTCCCGCC

Downstream 100 bases:

>100_bases
GCGGCACTAAAAGCACTTCATAGCGCTGCCGCGGAAATGTCTTCGTCAGCCGCTACAATGCAAACCTCACGCGCCGGCAT
CGCTTGCCGGCGTTGTCTTT

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MLRIISANLNGVRSAASKGFFDWMGKQDADFVCVQELKCAQDDMTPEFLAPHGYHGVFQHAVKKGYSGAGLYTRHKPDEV
IVGFDNGEFDAEGRYVEARYGKLSVISVYVPSGSSGEERQQAKFRFMDLFMEHLKDLRHEKGREVVLCGDVNIVHKEIDI
KNWKGNQKNSGCLPEERAWLTKLFDEVGYVDVFRTLDQRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGIAATARRASIF
KDIKFSDHAPLTIDYDTKPHW

Sequences:

>Translated_261_residues
MLRIISANLNGVRSAASKGFFDWMGKQDADFVCVQELKCAQDDMTPEFLAPHGYHGVFQHAVKKGYSGAGLYTRHKPDEV
IVGFDNGEFDAEGRYVEARYGKLSVISVYVPSGSSGEERQQAKFRFMDLFMEHLKDLRHEKGREVVLCGDVNIVHKEIDI
KNWKGNQKNSGCLPEERAWLTKLFDEVGYVDVFRTLDQRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGIAATARRASIF
KDIKFSDHAPLTIDYDTKPHW
>Mature_261_residues
MLRIISANLNGVRSAASKGFFDWMGKQDADFVCVQELKCAQDDMTPEFLAPHGYHGVFQHAVKKGYSGAGLYTRHKPDEV
IVGFDNGEFDAEGRYVEARYGKLSVISVYVPSGSSGEERQQAKFRFMDLFMEHLKDLRHEKGREVVLCGDVNIVHKEIDI
KNWKGNQKNSGCLPEERAWLTKLFDEVGYVDVFRTLDQRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGIAATARRASIF
KDIKFSDHAPLTIDYDTKPHW

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=255, Percent_Identity=35.6862745098039, Blast_Score=164, Evalue=6e-41,
Organism=Homo sapiens, GI18375503, Length=255, Percent_Identity=35.6862745098039, Blast_Score=164, Evalue=6e-41,
Organism=Homo sapiens, GI18375501, Length=255, Percent_Identity=35.6862745098039, Blast_Score=164, Evalue=6e-41,
Organism=Escherichia coli, GI1788046, Length=269, Percent_Identity=32.3420074349442, Blast_Score=98, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71989536, Length=259, Percent_Identity=30.1158301158301, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI221330655, Length=253, Percent_Identity=35.9683794466403, Blast_Score=150, Evalue=6e-37,
Organism=Drosophila melanogaster, GI17136678, Length=253, Percent_Identity=35.9683794466403, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29863; Mature: 29863

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIISANLNGVRSAASKGFFDWMGKQDADFVCVQELKCAQDDMTPEFLAPHGYHGVFQH
CEEEEECCCCHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHCCCCCHHHCCCCCCHHHHHH
AVKKGYSGAGLYTRHKPDEVIVGFDNGEFDAEGRYVEARYGKLSVISVYVPSGSSGEERQ
HHHCCCCCCCCEECCCCCEEEEECCCCCCCCCCCEEEEECCCEEEEEEEECCCCCCHHHH
QAKFRFMDLFMEHLKDLRHEKGREVVLCGDVNIVHKEIDIKNWKGNQKNSGCLPEERAWL
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCCCHHHHHH
TKLFDEVGYVDVFRTLDQRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGIAATARRASIF
HHHHHHCCHHHHHHHHHCCCCCCEEECCCCCEEHHCCCEEEEEEEECCCHHHHHHHHHHH
KDIKFSDHAPLTIDYDTKPHW
HHCCCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MLRIISANLNGVRSAASKGFFDWMGKQDADFVCVQELKCAQDDMTPEFLAPHGYHGVFQH
CEEEEECCCCHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHCCCCCHHHCCCCCCHHHHHH
AVKKGYSGAGLYTRHKPDEVIVGFDNGEFDAEGRYVEARYGKLSVISVYVPSGSSGEERQ
HHHCCCCCCCCEECCCCCEEEEECCCCCCCCCCCEEEEECCCEEEEEEEECCCCCCHHHH
QAKFRFMDLFMEHLKDLRHEKGREVVLCGDVNIVHKEIDIKNWKGNQKNSGCLPEERAWL
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCCCHHHHHH
TKLFDEVGYVDVFRTLDQRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGIAATARRASIF
HHHHHHCCHHHHHHHHHCCCCCCEEECCCCCEEHHCCCEEEEEEEECCCHHHHHHHHHHH
KDIKFSDHAPLTIDYDTKPHW
HHCCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]