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The map label for this gene is miaA

Identifier: 187918674

GI number: 187918674

Start: 880295

End: 881197

Strand: Direct

Name: miaA

Synonym: BH0821

Alternate gene names: 187918674

Gene position: 880295-881197 (Clockwise)

Preceding gene: 187918673

Following gene: 187918676

Centisome position: 95.44

GC content: 31.67

Gene sequence:

>903_bases
TTGAAGAGAAATAAAATAGTTTTTATATTTGGTCCTACGGCTGTAGGTAAGAGTGAGCTTTTACTCAATTTTCCAAAAGG
TGTAGCTGAAATAATTAATGTCGATTCTGTTCAAGTTTATAAGGAGTTCGATATTGCGTCTTGTAAGCCCAGCATTGAAT
TGAGAGCCCATGTAAAACATCATTTAGTTGATTTTTTAGAACCGATTGAAGAGTATAATCTTGGAATATTTTATAAGGAA
GCATGCGAAATAATAGAGAATTTAAGAGTGCAAAAGAAGCTTCCTGTATTTGTAGGTGGGTCGGCTTTTTATTTTAAGCA
TTTAAAGTATGGATTGCCCAGTACACCGCCTGTTTCTTCTGAAATAAGACTTCATATAAATAGTCTTTTTACTACAAGGG
GGAAGAATTATCTCTTAGAGGAACTTAAGAGAGTAGACTTTGAAAGATATGAGTCAATTAGTAAAAATGATATTTATAGG
ATTAAAAGATCGCTTGAAGTTTATTATCAAACAGGTATTCCAATTAGTCAATTTTTAAAAAGAGGGCAGATGCTTGCAAA
TGTTTTAGCTATTGGCTTGAGAAGGCCTATGGAAGAAATGAGGTCTAGGATAATATCTAGGGTCAAGAATATGATTGATT
GTGGATTGCTTGAAGAGATTAAGAGCTTATTAGGGAAAGGATACAATGAGACAACCCCAGCTTTTAAAGGAATAGGGTAT
CGTGAATTTTTGTTGTGGAAGAGTAGACCTTATTCTATGTTAAATGATATAATAAATTTAATAGTTAAGAATTCATTTTT
ATATGTAAAAAGGCAGATGACTTTTTTTGATAAAATTCCTAATGTTTTGTGGTTTCATCCAGATGATGACTTAAAAGACA
TTTTGGACTTAATTTTTGTTTAA

Upstream 100 bases:

>100_bases
ATTATTGATCAAATTTCTTTAGCAGAGCATACTATTTCTGATGAGAGAGTAGTAAGCCAAGCTTTTAATGATATTTTAAC
AGATCGATTTACGTATTCAA

Downstream 100 bases:

>100_bases
TAAGGAGATATGAGGCGTGCCTTGTGGAAGAAAAAGAAAATTACAAAAAATATCTACTCATAAGCGAAAGAAAAAGAGAA
GAAAGAATAGACATAAAAAG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKRNKIVFIFGPTAVGKSELLLNFPKGVAEIINVDSVQVYKEFDIASCKPSIELRAHVKHHLVDFLEPIEEYNLGIFYKE
ACEIIENLRVQKKLPVFVGGSAFYFKHLKYGLPSTPPVSSEIRLHINSLFTTRGKNYLLEELKRVDFERYESISKNDIYR
IKRSLEVYYQTGIPISQFLKRGQMLANVLAIGLRRPMEEMRSRIISRVKNMIDCGLLEEIKSLLGKGYNETTPAFKGIGY
REFLLWKSRPYSMLNDIINLIVKNSFLYVKRQMTFFDKIPNVLWFHPDDDLKDILDLIFV

Sequences:

>Translated_300_residues
MKRNKIVFIFGPTAVGKSELLLNFPKGVAEIINVDSVQVYKEFDIASCKPSIELRAHVKHHLVDFLEPIEEYNLGIFYKE
ACEIIENLRVQKKLPVFVGGSAFYFKHLKYGLPSTPPVSSEIRLHINSLFTTRGKNYLLEELKRVDFERYESISKNDIYR
IKRSLEVYYQTGIPISQFLKRGQMLANVLAIGLRRPMEEMRSRIISRVKNMIDCGLLEEIKSLLGKGYNETTPAFKGIGY
REFLLWKSRPYSMLNDIINLIVKNSFLYVKRQMTFFDKIPNVLWFHPDDDLKDILDLIFV
>Mature_300_residues
MKRNKIVFIFGPTAVGKSELLLNFPKGVAEIINVDSVQVYKEFDIASCKPSIELRAHVKHHLVDFLEPIEEYNLGIFYKE
ACEIIENLRVQKKLPVFVGGSAFYFKHLKYGLPSTPPVSSEIRLHINSLFTTRGKNYLLEELKRVDFERYESISKNDIYR
IKRSLEVYYQTGIPISQFLKRGQMLANVLAIGLRRPMEEMRSRIISRVKNMIDCGLLEEIKSLLGKGYNETTPAFKGIGY
REFLLWKSRPYSMLNDIINLIVKNSFLYVKRQMTFFDKIPNVLWFHPDDDLKDILDLIFV

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=265, Percent_Identity=28.3018867924528, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI1790613, Length=305, Percent_Identity=27.5409836065574, Blast_Score=126, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25144712, Length=278, Percent_Identity=28.7769784172662, Blast_Score=103, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6324848, Length=294, Percent_Identity=28.5714285714286, Blast_Score=110, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24649830, Length=266, Percent_Identity=29.6992481203008, Blast_Score=110, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_BORHD (B2S1G5)

Other databases:

- EMBL:   CP000048
- RefSeq:   YP_001884241.1
- EnsemblBacteria:   EBBORT00000006415
- GeneID:   6276577
- GenomeReviews:   CP000048_GR
- KEGG:   bhr:BH0821
- GeneTree:   EBGT00050000007306
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK14729
- BioCyc:   BHER314723:BH0821-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34971; Mature: 34971

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNKIVFIFGPTAVGKSELLLNFPKGVAEIINVDSVQVYKEFDIASCKPSIELRAHVKH
CCCCCEEEEECCCCCCCCHHHEECCHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHH
HLVDFLEPIEEYNLGIFYKEACEIIENLRVQKKLPVFVGGSAFYFKHLKYGLPSTPPVSS
HHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHCCCCCCCCCCC
EIRLHINSLFTTRGKNYLLEELKRVDFERYESISKNDIYRIKRSLEVYYQTGIPISQFLK
HHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHH
RGQMLANVLAIGLRRPMEEMRSRIISRVKNMIDCGLLEEIKSLLGKGYNETTPAFKGIGY
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHCCCH
REFLLWKSRPYSMLNDIINLIVKNSFLYVKRQMTFFDKIPNVLWFHPDDDLKDILDLIFV
HHHHEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCC
>Mature Secondary Structure
MKRNKIVFIFGPTAVGKSELLLNFPKGVAEIINVDSVQVYKEFDIASCKPSIELRAHVKH
CCCCCEEEEECCCCCCCCHHHEECCHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHH
HLVDFLEPIEEYNLGIFYKEACEIIENLRVQKKLPVFVGGSAFYFKHLKYGLPSTPPVSS
HHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHCCCCCCCCCCC
EIRLHINSLFTTRGKNYLLEELKRVDFERYESISKNDIYRIKRSLEVYYQTGIPISQFLK
HHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHH
RGQMLANVLAIGLRRPMEEMRSRIISRVKNMIDCGLLEEIKSLLGKGYNETTPAFKGIGY
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHCCCH
REFLLWKSRPYSMLNDIINLIVKNSFLYVKRQMTFFDKIPNVLWFHPDDDLKDILDLIFV
HHHHEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA