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The map label for this gene is pnp [H]

Identifier: 187918659

GI number: 187918659

Start: 862446

End: 864629

Strand: Direct

Name: pnp [H]

Synonym: BH0805

Alternate gene names: 187918659

Gene position: 862446-864629 (Clockwise)

Preceding gene: 187918658

Following gene: 187918660

Centisome position: 93.51

GC content: 35.67

Gene sequence:

>2184_bases
TTGGACTTAGAAAGTAATAAGGGGTTAATTTTGAGAAAAATTTTAAGATTGAAAGTTGGAAGGGAAGATTTAATTTTGGA
AACAGGATTATTGGCTAAGCAAGCAAATGGAGCGGTTCTTGCTACTTATGGTGGTTCTACTGTTCTTGCTACGGTCTGTT
GTTCAGATTCAGTTCGGGAAAATTTAGATTTTGTCCCTTTATCTGTTGAATATAATGAGAAGTATTATGCTGCTGGAAAA
ATTCCTGGTGGGTTTATTAAAAGAGAGGGTAAACCAAAAGATAAGGAAGTACTTGTTTCTAGATTAATAGATAGACCTAT
GAGGCCGCTTTTTGATAAGAGGTTTGGTAGAGAAATTCAAGTTGTTCCAACAACTTTGTCTACAGATCAGATGAATCCCC
CTGATATTGTTGGGATGAATGCTGCTTTTGCAGCCGTTTTTTTATCGGATATTCCGTTTAATGGTCCAATTGCAGCTGTT
AGGCTGGCTTATTTAAATAATGAGTTTATAGTAAATCCTTCTTTTGATGAGATACAAGATTCTATTCTAGATATTGTTGT
TGCAGGAAGTTTGGATGGTATTACAATGGTTGAAGGTGGTGCTAATGAGGTTAGCGAGGAAGTATTGCTTTCTGCTATAG
ATAAAGCTTATGAATACATTAAACAAATTTGTAATCTTCAGAAAGAATTTGTATCTATAATAGGTGAGAGAGAGAAATTA
CCACTTGCTTATGAAGAAGGAGTATTTGAATTTAAGGATGAACTTAAAAATTTAATTTACTCTGAACTTAAGGATGCTTG
TTTTGTTAAGGGCAAACTTAATAGAGATAAGGCTATAAAATTAGTTAAGCAGAAAGCTTATGAACATTTTTCTTCTATAA
GTCAGGTCAATGAAGATAATGAATTCCTTTTTTATAAAGCTTTTGATGATTTTGAGAGAGAAATTGTTAGAAAATCAATC
CTTGAGAATAATCTTAGAACCGATGGGCGTACTTCTACACAGATAAGAGATATTGTTGCTGAAGTTGATCTTTTAAAGAG
AACCCATGGTTCTTCTCTTTTTACAAGAGGTGAAACCCAGGCATTAGCCGTAACGACTTTAGGCACAAGTATTGATGAGC
AAATAATGGATGATATTGATGGTGATAAGCGTCTTAATTTTATGCTTCATTATAATTTCCCTCCGTTTTCTGTTGGCGAG
ACAGGTAGATTGATGACTGGCAGGCGTGAGGTTGGGCATGGGCATTTAGCTCAAAGGTCTTTGGAGGCCATGTTACCTAA
GAAAGATGATTTTCCATATACTATTAGAGTGGTATCTGAGATATTAGAATCAAATGGCTCATCATCAATGGCTACAGTAT
GTTCTGGGAGTATGTCTTTAATGGCTGCTGGGGTTCCTGTTAAGGAGCAGGTTGCAGGAATAGCTATGGGATTAATTAGT
GATGGCGATAAATATGTTGTCTTGAGCGATATTCTTGGAGAAGAAGATCATTTAGGTGATATGGATTTTAAGGTTGCAGG
AACTAAGAATGGGATTACTGGCTTTCAAATGGACATTAAGATTTCAAATGTTACAAAGCAATTGATGAAGGATGCTCTTG
AACAGGCACGAATTGGAAGAATGCATATTCTATCTATTATGGATTCTGTAATTTCAAGATCAAGAGACGATATATCTGTT
AATGCACCTAAGATTGTTCAGTTGCAAATTGATATTGATAAAATTTCTCTTGTTATTGGCTCTACTGGCAAGACAGTTAA
GGCAATTACGGATGAGTTTGAGGTTAGGGTGCAAATTGAGCAAGATGGTAGGATTACCCTTTTTGGAACTGATAGCTTAA
AGATGCAAAAAGCTAAGGCAAAGATAGAGAGTATTGTAAGAGAACCTAAAATTGGTGAGATTTATGACGGGATCGTTAAA
AAGATTAACAGTTTTGGAGCTTTCATTGAACTTACTCCTATTAAGGAAGGCTTTTTAAGCAACCGAGCAAGATCGAGGGA
TGATAGATATGGTGATATGAGGCATTCTAGATATGGCAGTGGCCGGCATTCTAGATATGGTAGGGATAATAGGAATACAT
TTGGTATGAATCCTCCAAGATTAGAGGAGGGCCAAATTGTGAAGGTCAAAATATCTGATATCGATAAGTTTGGCAAGATT
GAGCTTGAATTAGTTAGAGATTAA

Upstream 100 bases:

>100_bases
TCTAAGAGAGGTTTGTTAAAGTTGGTTGGTCAGAGAAGGAGTTTGTTAAGATATTATCAGAAAAAAAATTTGGAAGCTTA
CAGAACCTTAATAGCTAAAC

Downstream 100 bases:

>100_bases
TAAGTGTTATGAAATTTGTAAATTATATAAATAATATTTTTAAAAATAAGCTTGTTTTTATGAGCTTATTTTTCTTTTTC
TCATGTCTGACTAGTAGAGA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 727; Mature: 727

Protein sequence:

>727_residues
MDLESNKGLILRKILRLKVGREDLILETGLLAKQANGAVLATYGGSTVLATVCCSDSVRENLDFVPLSVEYNEKYYAAGK
IPGGFIKREGKPKDKEVLVSRLIDRPMRPLFDKRFGREIQVVPTTLSTDQMNPPDIVGMNAAFAAVFLSDIPFNGPIAAV
RLAYLNNEFIVNPSFDEIQDSILDIVVAGSLDGITMVEGGANEVSEEVLLSAIDKAYEYIKQICNLQKEFVSIIGEREKL
PLAYEEGVFEFKDELKNLIYSELKDACFVKGKLNRDKAIKLVKQKAYEHFSSISQVNEDNEFLFYKAFDDFEREIVRKSI
LENNLRTDGRTSTQIRDIVAEVDLLKRTHGSSLFTRGETQALAVTTLGTSIDEQIMDDIDGDKRLNFMLHYNFPPFSVGE
TGRLMTGRREVGHGHLAQRSLEAMLPKKDDFPYTIRVVSEILESNGSSSMATVCSGSMSLMAAGVPVKEQVAGIAMGLIS
DGDKYVVLSDILGEEDHLGDMDFKVAGTKNGITGFQMDIKISNVTKQLMKDALEQARIGRMHILSIMDSVISRSRDDISV
NAPKIVQLQIDIDKISLVIGSTGKTVKAITDEFEVRVQIEQDGRITLFGTDSLKMQKAKAKIESIVREPKIGEIYDGIVK
KINSFGAFIELTPIKEGFLSNRARSRDDRYGDMRHSRYGSGRHSRYGRDNRNTFGMNPPRLEEGQIVKVKISDIDKFGKI
ELELVRD

Sequences:

>Translated_727_residues
MDLESNKGLILRKILRLKVGREDLILETGLLAKQANGAVLATYGGSTVLATVCCSDSVRENLDFVPLSVEYNEKYYAAGK
IPGGFIKREGKPKDKEVLVSRLIDRPMRPLFDKRFGREIQVVPTTLSTDQMNPPDIVGMNAAFAAVFLSDIPFNGPIAAV
RLAYLNNEFIVNPSFDEIQDSILDIVVAGSLDGITMVEGGANEVSEEVLLSAIDKAYEYIKQICNLQKEFVSIIGEREKL
PLAYEEGVFEFKDELKNLIYSELKDACFVKGKLNRDKAIKLVKQKAYEHFSSISQVNEDNEFLFYKAFDDFEREIVRKSI
LENNLRTDGRTSTQIRDIVAEVDLLKRTHGSSLFTRGETQALAVTTLGTSIDEQIMDDIDGDKRLNFMLHYNFPPFSVGE
TGRLMTGRREVGHGHLAQRSLEAMLPKKDDFPYTIRVVSEILESNGSSSMATVCSGSMSLMAAGVPVKEQVAGIAMGLIS
DGDKYVVLSDILGEEDHLGDMDFKVAGTKNGITGFQMDIKISNVTKQLMKDALEQARIGRMHILSIMDSVISRSRDDISV
NAPKIVQLQIDIDKISLVIGSTGKTVKAITDEFEVRVQIEQDGRITLFGTDSLKMQKAKAKIESIVREPKIGEIYDGIVK
KINSFGAFIELTPIKEGFLSNRARSRDDRYGDMRHSRYGSGRHSRYGRDNRNTFGMNPPRLEEGQIVKVKISDIDKFGKI
ELELVRD
>Mature_727_residues
MDLESNKGLILRKILRLKVGREDLILETGLLAKQANGAVLATYGGSTVLATVCCSDSVRENLDFVPLSVEYNEKYYAAGK
IPGGFIKREGKPKDKEVLVSRLIDRPMRPLFDKRFGREIQVVPTTLSTDQMNPPDIVGMNAAFAAVFLSDIPFNGPIAAV
RLAYLNNEFIVNPSFDEIQDSILDIVVAGSLDGITMVEGGANEVSEEVLLSAIDKAYEYIKQICNLQKEFVSIIGEREKL
PLAYEEGVFEFKDELKNLIYSELKDACFVKGKLNRDKAIKLVKQKAYEHFSSISQVNEDNEFLFYKAFDDFEREIVRKSI
LENNLRTDGRTSTQIRDIVAEVDLLKRTHGSSLFTRGETQALAVTTLGTSIDEQIMDDIDGDKRLNFMLHYNFPPFSVGE
TGRLMTGRREVGHGHLAQRSLEAMLPKKDDFPYTIRVVSEILESNGSSSMATVCSGSMSLMAAGVPVKEQVAGIAMGLIS
DGDKYVVLSDILGEEDHLGDMDFKVAGTKNGITGFQMDIKISNVTKQLMKDALEQARIGRMHILSIMDSVISRSRDDISV
NAPKIVQLQIDIDKISLVIGSTGKTVKAITDEFEVRVQIEQDGRITLFGTDSLKMQKAKAKIESIVREPKIGEIYDGIVK
KINSFGAFIELTPIKEGFLSNRARSRDDRYGDMRHSRYGSGRHSRYGRDNRNTFGMNPPRLEEGQIVKVKISDIDKFGKI
ELELVRD

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=658, Percent_Identity=35.258358662614, Blast_Score=401, Evalue=1e-111,
Organism=Escherichia coli, GI145693187, Length=721, Percent_Identity=42.4410540915395, Blast_Score=565, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI115534063, Length=613, Percent_Identity=35.0734094616639, Blast_Score=355, Evalue=4e-98,
Organism=Drosophila melanogaster, GI281362905, Length=663, Percent_Identity=36.1990950226244, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24651641, Length=663, Percent_Identity=36.1990950226244, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24651643, Length=663, Percent_Identity=36.1990950226244, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI161079377, Length=610, Percent_Identity=36.7213114754098, Blast_Score=368, Evalue=1e-102,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 81084; Mature: 81084

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLESNKGLILRKILRLKVGREDLILETGLLAKQANGAVLATYGGSTVLATVCCSDSVRE
CCCCCCCCHHHHHHHHHHCCCHHHEEECCCHHCCCCCCEEEEECCCEEEEEECCCHHHHC
NLDFVPLSVEYNEKYYAAGKIPGGFIKREGKPKDKEVLVSRLIDRPMRPLFDKRFGREIQ
CCCEEEEEEEECCEEEEECCCCCHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCEEE
VVPTTLSTDQMNPPDIVGMNAAFAAVFLSDIPFNGPIAAVRLAYLNNEFIVNPSFDEIQD
EEEEEECCCCCCCCCEEECCHHHHHHHHHCCCCCCCHHHEEEEEECCEEEECCCHHHHHH
SILDIVVAGSLDGITMVEGGANEVSEEVLLSAIDKAYEYIKQICNLQKEFVSIIGEREKL
HHHHEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PLAYEEGVFEFKDELKNLIYSELKDACFVKGKLNRDKAIKLVKQKAYEHFSSISQVNEDN
CCHHHCCHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
EFLFYKAFDDFEREIVRKSILENNLRTDGRTSTQIRDIVAEVDLLKRTHGSSLFTRGETQ
CEEEEECHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCC
ALAVTTLGTSIDEQIMDDIDGDKRLNFMLHYNFPPFSVGETGRLMTGRREVGHGHLAQRS
EEEEEECCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCEECCCHHCCCCHHHHHH
LEAMLPKKDDFPYTIRVVSEILESNGSSSMATVCSGSMSLMAAGVPVKEQVAGIAMGLIS
HHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCHHEEECCCCHHHHHHHHHHHHCC
DGDKYVVLSDILGEEDHLGDMDFKVAGTKNGITGFQMDIKISNVTKQLMKDALEQARIGR
CCCCEEEEHHHCCCCCCCCCCCEEEECCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCH
MHILSIMDSVISRSRDDISVNAPKIVQLQIDIDKISLVIGSTGKTVKAITDEFEVRVQIE
HHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEHEEEEEEECCCCCCHHHCCCCEEEEEEEE
QDGRITLFGTDSLKMQKAKAKIESIVREPKIGEIYDGIVKKINSFGAFIELTPIKEGFLS
CCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHH
NRARSRDDRYGDMRHSRYGSGRHSRYGRDNRNTFGMNPPRLEEGQIVKVKISDIDKFGKI
CHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCE
ELELVRD
EEEEECC
>Mature Secondary Structure
MDLESNKGLILRKILRLKVGREDLILETGLLAKQANGAVLATYGGSTVLATVCCSDSVRE
CCCCCCCCHHHHHHHHHHCCCHHHEEECCCHHCCCCCCEEEEECCCEEEEEECCCHHHHC
NLDFVPLSVEYNEKYYAAGKIPGGFIKREGKPKDKEVLVSRLIDRPMRPLFDKRFGREIQ
CCCEEEEEEEECCEEEEECCCCCHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCEEE
VVPTTLSTDQMNPPDIVGMNAAFAAVFLSDIPFNGPIAAVRLAYLNNEFIVNPSFDEIQD
EEEEEECCCCCCCCCEEECCHHHHHHHHHCCCCCCCHHHEEEEEECCEEEECCCHHHHHH
SILDIVVAGSLDGITMVEGGANEVSEEVLLSAIDKAYEYIKQICNLQKEFVSIIGEREKL
HHHHEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PLAYEEGVFEFKDELKNLIYSELKDACFVKGKLNRDKAIKLVKQKAYEHFSSISQVNEDN
CCHHHCCHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
EFLFYKAFDDFEREIVRKSILENNLRTDGRTSTQIRDIVAEVDLLKRTHGSSLFTRGETQ
CEEEEECHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCC
ALAVTTLGTSIDEQIMDDIDGDKRLNFMLHYNFPPFSVGETGRLMTGRREVGHGHLAQRS
EEEEEECCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCEECCCHHCCCCHHHHHH
LEAMLPKKDDFPYTIRVVSEILESNGSSSMATVCSGSMSLMAAGVPVKEQVAGIAMGLIS
HHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCHHEEECCCCHHHHHHHHHHHHCC
DGDKYVVLSDILGEEDHLGDMDFKVAGTKNGITGFQMDIKISNVTKQLMKDALEQARIGR
CCCCEEEEHHHCCCCCCCCCCCEEEECCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCH
MHILSIMDSVISRSRDDISVNAPKIVQLQIDIDKISLVIGSTGKTVKAITDEFEVRVQIE
HHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEHEEEEEEECCCCCCHHHCCCCEEEEEEEE
QDGRITLFGTDSLKMQKAKAKIESIVREPKIGEIYDGIVKKINSFGAFIELTPIKEGFLS
CCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHH
NRARSRDDRYGDMRHSRYGSGRHSRYGRDNRNTFGMNPPRLEEGQIVKVKISDIDKFGKI
CHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCE
ELELVRD
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA