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The map label for this gene is tilS [H]

Identifier: 187918640

GI number: 187918640

Start: 835973

End: 837292

Strand: Direct

Name: tilS [H]

Synonym: BH0788

Alternate gene names: 187918640

Gene position: 835973-837292 (Clockwise)

Preceding gene: 187918639

Following gene: 187918641

Centisome position: 90.64

GC content: 27.73

Gene sequence:

>1320_bases
TTGATGTTTTGGAATGATATAGTAACAAAAATAGAAAGTTTTTACTTTAAAAATTCTCTGCCCAAAGGAAAATCAATTGT
TGCTTTTTCTGGAGGTGCAGATTCTACTACTTTGCTATTGGGTTTAAAGGAGTATTTAAATAATAATATTGTTGCATTTT
ATTTTGCGCATTACATAAGACCGGAGTTTGAACAAAATTTGGAAATAGAACACATAAAAAAGTTTTGTAATTTTCATAAT
ATTACTTTTCAAATAAAGAGGTGTAGTGTTGATATAAAGAGAGAGTCTAGTCAGCTTGGTATGTCAGTTGAGGAGTTGGC
TAGAAAATATCGTTATAATGCTTTATTGGAAGCTCTTAAGGAAAATGAGGCTAGGTATATTGCTCTTGCTCATAATAAAA
ATGATCAATTTGAGACTTTAGTGATGAGATTTTTCCAGGGTTCGTTTTTGGATGGATTTTCTGGTATTCCAATTATCAAT
GGCAATATTATTAGACCTTTACTTGAGGTATCAAGAGAGGAGATTGAACAATTTCTTTCTTTAAATAATATTGTTTACTT
TGTTGATAGCACTAACCATGAAGATTTGTATTTAAGAAATAGAATTAGAAATAATCTTATGCCAGTTATCGGAAAAATTT
TTAAGGGATATGAAAGGGGTTTGGAAAGAATATCTGATTTTTCAAGTGATCTTGTAGATTATTTTGATAAAAAGGATTTT
CTCCCTTATAGTAAGGGTAATTATTATTATTCTTTTGATGCTATGACTTTTTTCAGTCTACCAAAATACTTGGTTTTTAG
AAGTATTTTTAAGATTTTAAATTTGGAAGGCATTGTGTCAAATTTATCATATGGAGCTCTAAATGAGATTTTTAGGGTAA
ATCCTGCTAATAAAAGAAGTAAAATTTTACTTAGAACCAATGAGTTTTTTTTAGAAAAGCGTCAAGATAAGGTTAACCTT
ATCTTTAGGAGAGATGAAGAGATGCATGAGCCTTTTGATTTTTTCTTAAGAGTTAATGAATATCATAGTTTATCTTTGGG
TAAGATTTTGTTAAAATATTTGGAGTGTGAGGATAATTCTATACCAACATTGAGATGTTGTTCTTATGAGTTTAGGCATA
GTTTTTTTAAAAATAAATTACAATCAAAGAGATTCTTTTCTAAGTTTGTAAGATATAATCCACTTTATTTAATGTTGTTA
GTGTTAAATGATAAGATAATTGGAATTATTGATTTAAATACTTTAAACTTAATGTGGAGTGATAAGAGCATTATTAAAAA
AATTAATATATCTTTGTTCGGAGGATTTTTAAAGGAATGA

Upstream 100 bases:

>100_bases
AGCGTATTAAAAGTGTATTTGAGTTTTTAAGTGAAGAGATATTAGGCATTGATGAGTCTAATTTTGAACATAAAGTTGCA
ACTATTAATTCTAGTAGTTT

Downstream 100 bases:

>100_bases
ATATTAACAATAATAATTTGAATAATAATGGTAAATCTAATAATAAAAAGAAAAATAAGAATTGGATTTTGATTCTTGTT
ATAGTCTTCTTAATTTTAGC

Product: tRNA(Ile)-lysidine synthetase TilS

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MMFWNDIVTKIESFYFKNSLPKGKSIVAFSGGADSTTLLLGLKEYLNNNIVAFYFAHYIRPEFEQNLEIEHIKKFCNFHN
ITFQIKRCSVDIKRESSQLGMSVEELARKYRYNALLEALKENEARYIALAHNKNDQFETLVMRFFQGSFLDGFSGIPIIN
GNIIRPLLEVSREEIEQFLSLNNIVYFVDSTNHEDLYLRNRIRNNLMPVIGKIFKGYERGLERISDFSSDLVDYFDKKDF
LPYSKGNYYYSFDAMTFFSLPKYLVFRSIFKILNLEGIVSNLSYGALNEIFRVNPANKRSKILLRTNEFFLEKRQDKVNL
IFRRDEEMHEPFDFFLRVNEYHSLSLGKILLKYLECEDNSIPTLRCCSYEFRHSFFKNKLQSKRFFSKFVRYNPLYLMLL
VLNDKIIGIIDLNTLNLMWSDKSIIKKINISLFGGFLKE

Sequences:

>Translated_439_residues
MMFWNDIVTKIESFYFKNSLPKGKSIVAFSGGADSTTLLLGLKEYLNNNIVAFYFAHYIRPEFEQNLEIEHIKKFCNFHN
ITFQIKRCSVDIKRESSQLGMSVEELARKYRYNALLEALKENEARYIALAHNKNDQFETLVMRFFQGSFLDGFSGIPIIN
GNIIRPLLEVSREEIEQFLSLNNIVYFVDSTNHEDLYLRNRIRNNLMPVIGKIFKGYERGLERISDFSSDLVDYFDKKDF
LPYSKGNYYYSFDAMTFFSLPKYLVFRSIFKILNLEGIVSNLSYGALNEIFRVNPANKRSKILLRTNEFFLEKRQDKVNL
IFRRDEEMHEPFDFFLRVNEYHSLSLGKILLKYLECEDNSIPTLRCCSYEFRHSFFKNKLQSKRFFSKFVRYNPLYLMLL
VLNDKIIGIIDLNTLNLMWSDKSIIKKINISLFGGFLKE
>Mature_439_residues
MMFWNDIVTKIESFYFKNSLPKGKSIVAFSGGADSTTLLLGLKEYLNNNIVAFYFAHYIRPEFEQNLEIEHIKKFCNFHN
ITFQIKRCSVDIKRESSQLGMSVEELARKYRYNALLEALKENEARYIALAHNKNDQFETLVMRFFQGSFLDGFSGIPIIN
GNIIRPLLEVSREEIEQFLSLNNIVYFVDSTNHEDLYLRNRIRNNLMPVIGKIFKGYERGLERISDFSSDLVDYFDKKDF
LPYSKGNYYYSFDAMTFFSLPKYLVFRSIFKILNLEGIVSNLSYGALNEIFRVNPANKRSKILLRTNEFFLEKRQDKVNL
IFRRDEEMHEPFDFFLRVNEYHSLSLGKILLKYLECEDNSIPTLRCCSYEFRHSFFKNKLQSKRFFSKFVRYNPLYLMLL
VLNDKIIGIIDLNTLNLMWSDKSIIKKINISLFGGFLKE

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=211, Percent_Identity=25.5924170616114, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 51924; Mature: 51924

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMFWNDIVTKIESFYFKNSLPKGKSIVAFSGGADSTTLLLGLKEYLNNNIVAFYFAHYIR
CCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEHHHHHHC
PEFEQNLEIEHIKKFCNFHNITFQIKRCSVDIKRESSQLGMSVEELARKYRYNALLEALK
CHHHCCCCHHHHHHHHCCCCEEEEEEEEEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHH
ENEARYIALAHNKNDQFETLVMRFFQGSFLDGFSGIPIINGNIIRPLLEVSREEIEQFLS
CCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHH
LNNIVYFVDSTNHEDLYLRNRIRNNLMPVIGKIFKGYERGLERISDFSSDLVDYFDKKDF
HCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LPYSKGNYYYSFDAMTFFSLPKYLVFRSIFKILNLEGIVSNLSYGALNEIFRVNPANKRS
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCC
KILLRTNEFFLEKRQDKVNLIFRRDEEMHEPFDFFLRVNEYHSLSLGKILLKYLECEDNS
EEEEEEHHHHHHHCCCCEEEEEECCHHHCCCHHHHEEECCCCCCHHHHHHHHHHHCCCCC
IPTLRCCSYEFRHSFFKNKLQSKRFFSKFVRYNPLYLMLLVLNDKIIGIIDLNTLNLMWS
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEECCCCEEEEEEECEEEEEEC
DKSIIKKINISLFGGFLKE
CHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMFWNDIVTKIESFYFKNSLPKGKSIVAFSGGADSTTLLLGLKEYLNNNIVAFYFAHYIR
CCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEHHHHHHC
PEFEQNLEIEHIKKFCNFHNITFQIKRCSVDIKRESSQLGMSVEELARKYRYNALLEALK
CHHHCCCCHHHHHHHHCCCCEEEEEEEEEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHH
ENEARYIALAHNKNDQFETLVMRFFQGSFLDGFSGIPIINGNIIRPLLEVSREEIEQFLS
CCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHH
LNNIVYFVDSTNHEDLYLRNRIRNNLMPVIGKIFKGYERGLERISDFSSDLVDYFDKKDF
HCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LPYSKGNYYYSFDAMTFFSLPKYLVFRSIFKILNLEGIVSNLSYGALNEIFRVNPANKRS
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCC
KILLRTNEFFLEKRQDKVNLIFRRDEEMHEPFDFFLRVNEYHSLSLGKILLKYLECEDNS
EEEEEEHHHHHHHCCCCEEEEEECCHHHCCCHHHHEEECCCCCCHHHHHHHHHHHCCCCC
IPTLRCCSYEFRHSFFKNKLQSKRFFSKFVRYNPLYLMLLVLNDKIIGIIDLNTLNLMWS
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEECCCCEEEEEEECEEEEEEC
DKSIIKKINISLFGGFLKE
CHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA