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The map label for this gene is truA

Identifier: 187917895

GI number: 187917895

Start: 11746

End: 12483

Strand: Direct

Name: truA

Synonym: BH0012

Alternate gene names: 187917895

Gene position: 11746-12483 (Clockwise)

Preceding gene: 187917894

Following gene: 187917896

Centisome position: 1.27

GC content: 27.24

Gene sequence:

>738_bases
ATGAAAAAAATACTTGCAGAAATAGCATATGATGGTTCGCTATACTATGGATTTCAAATCCAACCAACAAAACCAACAAT
TCAAGGAGAAATTGAAAAGGCATTAGAGAAAATAAGTAAAACAAAGGTTAAAGTCCATTCAGCAGGCAGAACAGACAAAG
GAGTTCATGCAAGAGGACAAATAATATCTTTTTATATAAGAATAAATATTAAACTTAAAAATCTAAAGACAGCAATAAAT
TCTCTCTTAAGAAAAGATATTAGAATAATAAAATTAAAATATGTAGCAGATGAATTTCAACCTAGGTTTAATGCCAAGAG
AAGAAAGTATAGCTATTACATACTCAACAATGAAAACCATTACCCTTGGGAAGGGTATCAAGCTTATTATGTAAAGAAAA
AACTAAATATTAACAGACTAAATGAAATGGCTAAAATGCTAATTGGAATACACGATTTTACTACCTTTTCATGCATAAAA
GATCAGACAAACTCAAAATTAAAAAAAATTTACTTTGCTAGATTTAAGAAAAAAAATAAGCTTATAATCTTTGAAATCAT
AGGCTCCTCATTTTTATGGAAAATGGTAAGATCAATAGTAGGGACAATAATTGATATAGAGATAAAAAATGAACCTGTTT
ACACTTTTAAAAAAATTTTAAACTCAAAAAACAGAAAATTTACAAGAACAACAGCGCCTGCAAAAGCTTTATTTTTAGAT
AAGGTGTTTTATGAATAA

Upstream 100 bases:

>100_bases
CGGAATGGGAAAATCATCAAAAGAAAAACCTTCAATACTTTTGGATAAAATAAAAGAAGTTTATTTTAAAATCTCAAACG
AAATAAAAACTTTCAAATAA

Downstream 100 bases:

>100_bases
AAGCAAACTGCTTAAAAAATTGATACTTCTCAAAATACTTGAAAATAATATATCGGGAAATATTTATAGCAAACAAGAAG
AGCTTAAAAAACTCAAAAAT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MKKILAEIAYDGSLYYGFQIQPTKPTIQGEIEKALEKISKTKVKVHSAGRTDKGVHARGQIISFYIRINIKLKNLKTAIN
SLLRKDIRIIKLKYVADEFQPRFNAKRRKYSYYILNNENHYPWEGYQAYYVKKKLNINRLNEMAKMLIGIHDFTTFSCIK
DQTNSKLKKIYFARFKKKNKLIIFEIIGSSFLWKMVRSIVGTIIDIEIKNEPVYTFKKILNSKNRKFTRTTAPAKALFLD
KVFYE

Sequences:

>Translated_245_residues
MKKILAEIAYDGSLYYGFQIQPTKPTIQGEIEKALEKISKTKVKVHSAGRTDKGVHARGQIISFYIRINIKLKNLKTAIN
SLLRKDIRIIKLKYVADEFQPRFNAKRRKYSYYILNNENHYPWEGYQAYYVKKKLNINRLNEMAKMLIGIHDFTTFSCIK
DQTNSKLKKIYFARFKKKNKLIIFEIIGSSFLWKMVRSIVGTIIDIEIKNEPVYTFKKILNSKNRKFTRTTAPAKALFLD
KVFYE
>Mature_245_residues
MKKILAEIAYDGSLYYGFQIQPTKPTIQGEIEKALEKISKTKVKVHSAGRTDKGVHARGQIISFYIRINIKLKNLKTAIN
SLLRKDIRIIKLKYVADEFQPRFNAKRRKYSYYILNNENHYPWEGYQAYYVKKKLNINRLNEMAKMLIGIHDFTTFSCIK
DQTNSKLKKIYFARFKKKNKLIIFEIIGSSFLWKMVRSIVGTIIDIEIKNEPVYTFKKILNSKNRKFTRTTAPAKALFLD
KVFYE

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI199559785, Length=262, Percent_Identity=27.0992366412214, Blast_Score=71, Evalue=8e-13,
Organism=Escherichia coli, GI1788657, Length=242, Percent_Identity=31.404958677686, Blast_Score=142, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71985046, Length=264, Percent_Identity=26.1363636363636, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6321112, Length=228, Percent_Identity=28.9473684210526, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI281362146, Length=259, Percent_Identity=24.7104247104247, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648455, Length=259, Percent_Identity=24.7104247104247, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_BORHD (B2S1K1)

Other databases:

- EMBL:   CP000048
- RefSeq:   YP_001883458.1
- EnsemblBacteria:   EBBORT00000006537
- GeneID:   6277178
- GenomeReviews:   CP000048_GR
- KEGG:   bhr:BH0012
- GeneTree:   EBGT00050000007466
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   BHER314723:BH0012-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28871; Mature: 28871

Theoretical pI: Translated: 10.68; Mature: 10.68

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILAEIAYDGSLYYGFQIQPTKPTIQGEIEKALEKISKTKVKVHSAGRTDKGVHARGQ
CHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCE
IISFYIRINIKLKNLKTAINSLLRKDIRIIKLKYVADEFQPRFNAKRRKYSYYILNNENH
EEEEEEEEEEEEHHHHHHHHHHHHCCHHEEEEEEHHHHHCCCCCCCCEEEEEEEEECCCC
YPWEGYQAYYVKKKLNINRLNEMAKMLIGIHDFTTFSCIKDQTNSKLKKIYFARFKKKNK
CCCCCEEEEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
LIIFEIIGSSFLWKMVRSIVGTIIDIEIKNEPVYTFKKILNSKNRKFTRTTAPAKALFLD
EEEEEECCCHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHCCCCCEEEECCCCHHHHHHH
KVFYE
HHHCC
>Mature Secondary Structure
MKKILAEIAYDGSLYYGFQIQPTKPTIQGEIEKALEKISKTKVKVHSAGRTDKGVHARGQ
CHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCE
IISFYIRINIKLKNLKTAINSLLRKDIRIIKLKYVADEFQPRFNAKRRKYSYYILNNENH
EEEEEEEEEEEEHHHHHHHHHHHHCCHHEEEEEEHHHHHCCCCCCCCEEEEEEEEECCCC
YPWEGYQAYYVKKKLNINRLNEMAKMLIGIHDFTTFSCIKDQTNSKLKKIYFARFKKKNK
CCCCCEEEEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
LIIFEIIGSSFLWKMVRSIVGTIIDIEIKNEPVYTFKKILNSKNRKFTRTTAPAKALFLD
EEEEEECCCHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHCCCCCEEEECCCCHHHHHHH
KVFYE
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA