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The map label for this gene is hupB [H]

Identifier: 187736607

GI number: 187736607

Start: 2595940

End: 2597697

Strand: Direct

Name: hupB [H]

Synonym: Amuc_2129

Alternate gene names: 187736607

Gene position: 2595940-2597697 (Clockwise)

Preceding gene: 187736606

Following gene: 187736608

Centisome position: 97.44

GC content: 61.72

Gene sequence:

>1758_bases
ATGAGCAATTACACATCAGCGGACGTTCCGGAATCCAGCCGGGTGGTCATTGACCCGGTAACGCGCATCGAAGGCCACCT
GCGGGTGGAAATGGAGGCCGGGGACGGCGTCATCAAAAATGCCTGGACGTCCACCACGCAGTACCGTGGCATTGAGGTGA
TCGCCTGCAAGCGGGACCCCCGGGACGTTTGGGCTTTTGTGGAACGCATCTGCGGCGTCTGCACGGGTACGCACGCCATC
GCGGCCCTGGCTGCGGTGGAAGACGCGCTCCAATACCCGGTGCCCGTCCAGGCCCGCCTCATGCGAGACCTGGTCAGCGG
AGCCCTGGGCATCCAGGACCACGTCATCCATTTTTACCAGCTCCAGGCCATGGACTGGGTGGACGTGATGTCCGCCCTGA
AGGCGGACCCCGCGGAAACCGCCGCCATCGCCAAGTCCCTTTCCGACTGGCCCCTGTCCTCCGCCGCGTATTTCGCCGGA
GTGCAGAAGAAGCTGAAGGACTCCATCGCCTACGGGCAATATTCCATCTTTACGAACGGCTACTGGGGCCATCCGGCCTA
CAAGCTGCCGCCGGAGGTGAATCTGCTGGGCGTGGCGCATTATCTCCAGGCCCTGGTCTGGCAACGGGACATGATCAAAA
TCCACACCATCCTGGGCGGCAAGAACCCGCACCCGAACTTCCTGGTCGGCGGCATGGCCTGCGCCATCAACATGAACAAT
GACCAGGCCATCAACCAGTTTTCCCTGAGCTACCTGAAGCAGCTGGTGCAAACCTGCCACGATTTCATTCACAAAGTATA
TTATCCGGACATTGTAGCCATCGCCGGATTTTATAAGGACTACGCCCACATTGGCGCGTCCAACCCCAACTTTTTCTGCA
CTGGGGCTCCGTCTGAAATCAACACGGGCGCCCCCGCCGGCAAAGGCATGATCAAGCCCGGCGTGCTTCTGAATGGGGAT
TACAGGAACGTGCTTCCCTTTGACCAGGACAAGATCAGGGAATTCGTCACCAGCTCCTGGTACAGGTATACGGAAGGCAG
GGACGCGGGCCTGGCTCCGTATGACGGGGAGACGAATGCGGACTACAACGGCCCCAGGCCGCCCTACAAGTGGCTTTCCG
ACCACCCGCAGTACACCTGGGTGAAAGCCCCGCGCTATGACGGCCACGCGATGGCCGTGGGTCCGAACGCCCGCATGATG
ATTGGCTACGCCCAGGGGGTTCCCGCCATCGTGGAACGGGTGGATGCCGCCTGCGACAAGCTGGGCCTGCCGCGCGACAA
CGCCTTCATGAACTCCACGCTGGGCCGCGTATACGGCCGTGCGCTGGACGCCCTGATCAACGTGGACATGATGGTGGACC
AGCTCCAGGAAATGACGGACCGCATCAGGAACGGGGAAACCTCCACCTTCAACCCGGAAAAATGGGAACCGGAAACCTGG
CCCTCCTCCTGCAAGGGCGTGGGCTGGGTGGAAGCGCCCCGCGGCAGCCTGAGCCACTGGGTGCGGATTGAAAACGGCCA
GACGGTGAATTACCAGGCCGTAGTCCCCAGTACATGGAACAGTTCCGGACGGGACGCGGCGGGGCAGATGGGGCCATATG
AATATTCCCTGGCCCATACCGGGAAACACCCGCTGGTGGACCCCTCCCGCCCGGTGGAGCCCCTGCGCACCATTCACAGC
TATGACCCGTGCCAATCCTGCGCCGTCCATTTGTTTGACGAAGAAGGGGAGGCCATTCTGACCAGACAGGACCCGTGA

Upstream 100 bases:

>100_bases
AGGAGGAAGCAAACGTCTCCCTGCCCGCCTTCGGGGACGCTCCCGGCTCGCCCGACAAGGAAATTGAAGAATAGCCAGAA
GCCCTCACCACCAGATTACC

Downstream 100 bases:

>100_bases
AAACGACTCTTATCGATAACGACCTCACCCTGGCGGTGGAGGATGCCATTCCGGCCAGTCCGGTGTACGCTCCGGAAGAA
ATCCTGGCTCTGGCCGTGTG

Product: nickel-dependent hydrogenase large subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 585; Mature: 584

Protein sequence:

>585_residues
MSNYTSADVPESSRVVIDPVTRIEGHLRVEMEAGDGVIKNAWTSTTQYRGIEVIACKRDPRDVWAFVERICGVCTGTHAI
AALAAVEDALQYPVPVQARLMRDLVSGALGIQDHVIHFYQLQAMDWVDVMSALKADPAETAAIAKSLSDWPLSSAAYFAG
VQKKLKDSIAYGQYSIFTNGYWGHPAYKLPPEVNLLGVAHYLQALVWQRDMIKIHTILGGKNPHPNFLVGGMACAINMNN
DQAINQFSLSYLKQLVQTCHDFIHKVYYPDIVAIAGFYKDYAHIGASNPNFFCTGAPSEINTGAPAGKGMIKPGVLLNGD
YRNVLPFDQDKIREFVTSSWYRYTEGRDAGLAPYDGETNADYNGPRPPYKWLSDHPQYTWVKAPRYDGHAMAVGPNARMM
IGYAQGVPAIVERVDAACDKLGLPRDNAFMNSTLGRVYGRALDALINVDMMVDQLQEMTDRIRNGETSTFNPEKWEPETW
PSSCKGVGWVEAPRGSLSHWVRIENGQTVNYQAVVPSTWNSSGRDAAGQMGPYEYSLAHTGKHPLVDPSRPVEPLRTIHS
YDPCQSCAVHLFDEEGEAILTRQDP

Sequences:

>Translated_585_residues
MSNYTSADVPESSRVVIDPVTRIEGHLRVEMEAGDGVIKNAWTSTTQYRGIEVIACKRDPRDVWAFVERICGVCTGTHAI
AALAAVEDALQYPVPVQARLMRDLVSGALGIQDHVIHFYQLQAMDWVDVMSALKADPAETAAIAKSLSDWPLSSAAYFAG
VQKKLKDSIAYGQYSIFTNGYWGHPAYKLPPEVNLLGVAHYLQALVWQRDMIKIHTILGGKNPHPNFLVGGMACAINMNN
DQAINQFSLSYLKQLVQTCHDFIHKVYYPDIVAIAGFYKDYAHIGASNPNFFCTGAPSEINTGAPAGKGMIKPGVLLNGD
YRNVLPFDQDKIREFVTSSWYRYTEGRDAGLAPYDGETNADYNGPRPPYKWLSDHPQYTWVKAPRYDGHAMAVGPNARMM
IGYAQGVPAIVERVDAACDKLGLPRDNAFMNSTLGRVYGRALDALINVDMMVDQLQEMTDRIRNGETSTFNPEKWEPETW
PSSCKGVGWVEAPRGSLSHWVRIENGQTVNYQAVVPSTWNSSGRDAAGQMGPYEYSLAHTGKHPLVDPSRPVEPLRTIHS
YDPCQSCAVHLFDEEGEAILTRQDP
>Mature_584_residues
SNYTSADVPESSRVVIDPVTRIEGHLRVEMEAGDGVIKNAWTSTTQYRGIEVIACKRDPRDVWAFVERICGVCTGTHAIA
ALAAVEDALQYPVPVQARLMRDLVSGALGIQDHVIHFYQLQAMDWVDVMSALKADPAETAAIAKSLSDWPLSSAAYFAGV
QKKLKDSIAYGQYSIFTNGYWGHPAYKLPPEVNLLGVAHYLQALVWQRDMIKIHTILGGKNPHPNFLVGGMACAINMNND
QAINQFSLSYLKQLVQTCHDFIHKVYYPDIVAIAGFYKDYAHIGASNPNFFCTGAPSEINTGAPAGKGMIKPGVLLNGDY
RNVLPFDQDKIREFVTSSWYRYTEGRDAGLAPYDGETNADYNGPRPPYKWLSDHPQYTWVKAPRYDGHAMAVGPNARMMI
GYAQGVPAIVERVDAACDKLGLPRDNAFMNSTLGRVYGRALDALINVDMMVDQLQEMTDRIRNGETSTFNPEKWEPETWP
SSCKGVGWVEAPRGSLSHWVRIENGQTVNYQAVVPSTWNSSGRDAAGQMGPYEYSLAHTGKHPLVDPSRPVEPLRTIHSY
DPCQSCAVHLFDEEGEAILTRQDP

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=584, Percent_Identity=45.7191780821918, Blast_Score=533, Evalue=1e-153,
Organism=Escherichia coli, GI1789368, Length=582, Percent_Identity=42.9553264604811, Blast_Score=457, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 64824; Mature: 64693

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNYTSADVPESSRVVIDPVTRIEGHLRVEMEAGDGVIKNAWTSTTQYRGIEVIACKRDP
CCCCCCCCCCCCCCEEECCHHHCCCEEEEEEECCCCHHHHCCCCCCCCCCEEEEEECCCH
RDVWAFVERICGVCTGTHAIAALAAVEDALQYPVPVQARLMRDLVSGALGIQDHVIHFYQ
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHEEHHH
LQAMDWVDVMSALKADPAETAAIAKSLSDWPLSSAAYFAGVQKKLKDSIAYGQYSIFTNG
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECC
YWGHPAYKLPPEVNLLGVAHYLQALVWQRDMIKIHTILGGKNPHPNFLVGGMACAINMNN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEEEEEEEEECCC
DQAINQFSLSYLKQLVQTCHDFIHKVYYPDIVAIAGFYKDYAHIGASNPNFFCTGAPSEI
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCC
NTGAPAGKGMIKPGVLLNGDYRNVLPFDQDKIREFVTSSWYRYTEGRDAGLAPYDGETNA
CCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCC
DYNGPRPPYKWLSDHPQYTWVKAPRYDGHAMAVGPNARMMIGYAQGVPAIVERVDAACDK
CCCCCCCCHHHHCCCCCEEEEECCCCCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHH
LGLPRDNAFMNSTLGRVYGRALDALINVDMMVDQLQEMTDRIRNGETSTFNPEKWEPETW
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PSSCKGVGWVEAPRGSLSHWVRIENGQTVNYQAVVPSTWNSSGRDAAGQMGPYEYSLAHT
CCCCCCCCCEECCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEHCCC
GKHPLVDPSRPVEPLRTIHSYDPCQSCAVHLFDEEGEAILTRQDP
CCCCCCCCCCCHHHHHHHHCCCCHHHHHEEEECCCCCEEEECCCC
>Mature Secondary Structure 
SNYTSADVPESSRVVIDPVTRIEGHLRVEMEAGDGVIKNAWTSTTQYRGIEVIACKRDP
CCCCCCCCCCCCCEEECCHHHCCCEEEEEEECCCCHHHHCCCCCCCCCCEEEEEECCCH
RDVWAFVERICGVCTGTHAIAALAAVEDALQYPVPVQARLMRDLVSGALGIQDHVIHFYQ
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHEEHHH
LQAMDWVDVMSALKADPAETAAIAKSLSDWPLSSAAYFAGVQKKLKDSIAYGQYSIFTNG
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECC
YWGHPAYKLPPEVNLLGVAHYLQALVWQRDMIKIHTILGGKNPHPNFLVGGMACAINMNN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEEEEEEEEECCC
DQAINQFSLSYLKQLVQTCHDFIHKVYYPDIVAIAGFYKDYAHIGASNPNFFCTGAPSEI
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCC
NTGAPAGKGMIKPGVLLNGDYRNVLPFDQDKIREFVTSSWYRYTEGRDAGLAPYDGETNA
CCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCC
DYNGPRPPYKWLSDHPQYTWVKAPRYDGHAMAVGPNARMMIGYAQGVPAIVERVDAACDK
CCCCCCCCHHHHCCCCCEEEEECCCCCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHH
LGLPRDNAFMNSTLGRVYGRALDALINVDMMVDQLQEMTDRIRNGETSTFNPEKWEPETW
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PSSCKGVGWVEAPRGSLSHWVRIENGQTVNYQAVVPSTWNSSGRDAAGQMGPYEYSLAHT
CCCCCCCCCEECCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEHCCC
GKHPLVDPSRPVEPLRTIHSYDPCQSCAVHLFDEEGEAILTRQDP
CCCCCCCCCCCHHHHHHHHCCCCHHHHHEEEECCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3054886; 12597275; 8163174 [H]