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The map label for this gene is purA [H]

Identifier: 187736603

GI number: 187736603

Start: 2590141

End: 2591412

Strand: Direct

Name: purA [H]

Synonym: Amuc_2125

Alternate gene names: 187736603

Gene position: 2590141-2591412 (Clockwise)

Preceding gene: 187736602

Following gene: 187736605

Centisome position: 97.22

GC content: 59.51

Gene sequence:

>1272_bases
ATGAATACCATTATTATCGGCTCCCAATGGGGCGACGAAGGCAAGGGCAAAATGATCGATTTCCTGACGGAATCCGCCGA
CGTGGTGGCACGCGGCCAGGGCGGCAACAACGCCGGCCATACTGTAATTGCCAATGGAAAGAAATATATTCTCCACCTGG
TTCCCTCCGGCATCCTGTGGCCGGACAAGCTTTGCGTCATCGGTAATGGTGTGGTGCTGGACCCCATCGGCCTGGTGGAG
GAAATCAATGAACTCCGCGGCCAGGGCGTGACCATTACAAAAGACAACCTTCTTATCTCCGACCGCGCCCACGTGGTCCT
TCCCTTCCACAAGGAAATGGACGCCGCCCAGGAATCCGCCCTCGGCAAAAAAGCCATCGGCACCACCAAGCGCGGCATCG
GCCCCACTTACGCGGACAAGGCGCGCCGCATCGGCGTGCGCATGGCGGATATGAGGGATCCCGCGATTTTTGACGAAGTG
CTGCGCCGCCGCATCAGGATGGCGAATGCGGAAATGGAACGCATGGGCCTTCCCGCTATGGATGAAGAAAAAATGGTGGC
GGAAGTAAGCGCCGCCGCGGATGTGCTGCGCCCCCACATCACCAACACCATTCCGGTTATGCACGAAGCCGTGGCCTCCG
GAAAAAGCATTCTCTTTGAAGGGGCCCAGGGAGCTTACCTGGATGTGGACTTCGGCACCTATCCGTTCGTCACCTCCTCC
AACACCTCTTCCGCTGGCGCCTGCACCGGCACGGGTGTTCCTCCGCACAAAATTGACCGCATCATCGGCGTCTGCAAGGC
CTACACCACCCGCGTGGGCGCAGGCCCCTTCGTCACGGAGGATGAAGACATCTCCAACCATCTGCACAGCATGGGCCGCG
AATTCGGCGCCACCACGGGCCGCCCCCGCCGTTGCGGTTGGGCTGACGGCGTGCTGCTGCGCTTCTCCGCCATGTTCAAC
GGTTTTGATGAAATGGCCATGACCAACCTGGACGGCTACGACAAGTGCCCGGAAATTAAAATCTGCACGGGTTACGATCT
GGACGGGGAAATCCTGGCTTATCCGCCCGCCACAGTGGATGAATGGGAACGCTGCAAGCCCGTGTATGAAACGGTGCCCG
GCTGGATGCAGGACATTTCCTCCTGCCGTTCCTGGGAAGAGATTCCGGAACAGGCCAAGAAATTCGTCAAACGCATGAGC
GAGCTCATCGGCTGCCCCGTCACAACCGTGGGCGTAGGCCCCGACCGCGAACAGACGATTGCCGTGCAATAA

Upstream 100 bases:

>100_bases
CCGGGCTTCCCGCCACTGGTCAGGAAAACTTAACAATTCCGTAAGATTGTCTTGCCATAAGGCAGGAATCTCATTAAGGT
CTCCGAGTTCCATTTTTATC

Downstream 100 bases:

>100_bases
ACCTCGGCAGTTCTCGTTATTATTTCCTTCAAAGCAGTTCCGCATGCATGGCGGAACTGCTTTTTTATTGTCAGCTAATA
AAGGCCGAACACACGTTTCA

Product: Adenylosuccinate synthase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MNTIIIGSQWGDEGKGKMIDFLTESADVVARGQGGNNAGHTVIANGKKYILHLVPSGILWPDKLCVIGNGVVLDPIGLVE
EINELRGQGVTITKDNLLISDRAHVVLPFHKEMDAAQESALGKKAIGTTKRGIGPTYADKARRIGVRMADMRDPAIFDEV
LRRRIRMANAEMERMGLPAMDEEKMVAEVSAAADVLRPHITNTIPVMHEAVASGKSILFEGAQGAYLDVDFGTYPFVTSS
NTSSAGACTGTGVPPHKIDRIIGVCKAYTTRVGAGPFVTEDEDISNHLHSMGREFGATTGRPRRCGWADGVLLRFSAMFN
GFDEMAMTNLDGYDKCPEIKICTGYDLDGEILAYPPATVDEWERCKPVYETVPGWMQDISSCRSWEEIPEQAKKFVKRMS
ELIGCPVTTVGVGPDREQTIAVQ

Sequences:

>Translated_423_residues
MNTIIIGSQWGDEGKGKMIDFLTESADVVARGQGGNNAGHTVIANGKKYILHLVPSGILWPDKLCVIGNGVVLDPIGLVE
EINELRGQGVTITKDNLLISDRAHVVLPFHKEMDAAQESALGKKAIGTTKRGIGPTYADKARRIGVRMADMRDPAIFDEV
LRRRIRMANAEMERMGLPAMDEEKMVAEVSAAADVLRPHITNTIPVMHEAVASGKSILFEGAQGAYLDVDFGTYPFVTSS
NTSSAGACTGTGVPPHKIDRIIGVCKAYTTRVGAGPFVTEDEDISNHLHSMGREFGATTGRPRRCGWADGVLLRFSAMFN
GFDEMAMTNLDGYDKCPEIKICTGYDLDGEILAYPPATVDEWERCKPVYETVPGWMQDISSCRSWEEIPEQAKKFVKRMS
ELIGCPVTTVGVGPDREQTIAVQ
>Mature_423_residues
MNTIIIGSQWGDEGKGKMIDFLTESADVVARGQGGNNAGHTVIANGKKYILHLVPSGILWPDKLCVIGNGVVLDPIGLVE
EINELRGQGVTITKDNLLISDRAHVVLPFHKEMDAAQESALGKKAIGTTKRGIGPTYADKARRIGVRMADMRDPAIFDEV
LRRRIRMANAEMERMGLPAMDEEKMVAEVSAAADVLRPHITNTIPVMHEAVASGKSILFEGAQGAYLDVDFGTYPFVTSS
NTSSAGACTGTGVPPHKIDRIIGVCKAYTTRVGAGPFVTEDEDISNHLHSMGREFGATTGRPRRCGWADGVLLRFSAMFN
GFDEMAMTNLDGYDKCPEIKICTGYDLDGEILAYPPATVDEWERCKPVYETVPGWMQDISSCRSWEEIPEQAKKFVKRMS
ELIGCPVTTVGVGPDREQTIAVQ

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=425, Percent_Identity=44.7058823529412, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI34577063, Length=427, Percent_Identity=44.9648711943794, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI40316944, Length=395, Percent_Identity=43.7974683544304, Blast_Score=328, Evalue=6e-90,
Organism=Escherichia coli, GI1790620, Length=425, Percent_Identity=42.8235294117647, Blast_Score=375, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI25146555, Length=430, Percent_Identity=44.8837209302326, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25146553, Length=430, Percent_Identity=44.8837209302326, Blast_Score=362, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324109, Length=428, Percent_Identity=42.2897196261682, Blast_Score=365, Evalue=1e-102,
Organism=Drosophila melanogaster, GI21356033, Length=425, Percent_Identity=44.2352941176471, Blast_Score=363, Evalue=1e-100,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46048; Mature: 46048

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTIIIGSQWGDEGKGKMIDFLTESADVVARGQGGNNAGHTVIANGKKYILHLVPSGILW
CCEEEEECCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCEEEECCCEEEEEECCCCCCC
PDKLCVIGNGVVLDPIGLVEEINELRGQGVTITKDNLLISDRAHVVLPFHKEMDAAQESA
CCCEEEECCCEEECCHHHHHHHHHHCCCCEEEECCCEEEECCCEEEEECHHHHHHHHHHH
LGKKAIGTTKRGIGPTYADKARRIGVRMADMRDPAIFDEVLRRRIRMANAEMERMGLPAM
CCHHHCCCCCCCCCCCHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHCCHHHHCCCCCC
DEEKMVAEVSAAADVLRPHITNTIPVMHEAVASGKSILFEGAQGAYLDVDFGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCEEECC
NTSSAGACTGTGVPPHKIDRIIGVCKAYTTRVGAGPFVTEDEDISNHLHSMGREFGATTG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
RPRRCGWADGVLLRFSAMFNGFDEMAMTNLDGYDKCPEIKICTGYDLDGEILAYPPATVD
CCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCC
EWERCKPVYETVPGWMQDISSCRSWEEIPEQAKKFVKRMSELIGCPVTTVGVGPDREQTI
HHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEE
AVQ
ECC
>Mature Secondary Structure
MNTIIIGSQWGDEGKGKMIDFLTESADVVARGQGGNNAGHTVIANGKKYILHLVPSGILW
CCEEEEECCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCEEEECCCEEEEEECCCCCCC
PDKLCVIGNGVVLDPIGLVEEINELRGQGVTITKDNLLISDRAHVVLPFHKEMDAAQESA
CCCEEEECCCEEECCHHHHHHHHHHCCCCEEEECCCEEEECCCEEEEECHHHHHHHHHHH
LGKKAIGTTKRGIGPTYADKARRIGVRMADMRDPAIFDEVLRRRIRMANAEMERMGLPAM
CCHHHCCCCCCCCCCCHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHCCHHHHCCCCCC
DEEKMVAEVSAAADVLRPHITNTIPVMHEAVASGKSILFEGAQGAYLDVDFGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCEEECC
NTSSAGACTGTGVPPHKIDRIIGVCKAYTTRVGAGPFVTEDEDISNHLHSMGREFGATTG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
RPRRCGWADGVLLRFSAMFNGFDEMAMTNLDGYDKCPEIKICTGYDLDGEILAYPPATVD
CCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCC
EWERCKPVYETVPGWMQDISSCRSWEEIPEQAKKFVKRMSELIGCPVTTVGVGPDREQTI
HHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEE
AVQ
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA