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The map label for this gene is recF [H]

Identifier: 187735102

GI number: 187735102

Start: 705696

End: 706751

Strand: Direct

Name: recF [H]

Synonym: Amuc_0596

Alternate gene names: 187735102

Gene position: 705696-706751 (Clockwise)

Preceding gene: 187735101

Following gene: 187735104

Centisome position: 26.49

GC content: 63.16

Gene sequence:

>1056_bases
ATGATCTCCAGGCTGAAATTAATGGACTTCCGTTGCTACGGAAGCTTCTCCTGGCAGATTCCGCAGCAGGGGGCCATCAT
CCTGGGAAACAACGCCCGGGGAAAAACCAGCCTGCTGGAGGCCGTTTGCTTCCTGCTGCGCCTGCAATCCCCGCGCACGG
CCCGGACCGGGCCGCTGGTCTCCCACGGAAAACAATCCTTCGGCATCCGCGGAGAACTTCCGGGGCAAATCCGGCGCATC
CTGTGGGCGCCGGACGCTCCGGACCTCCGCGTCAACGGAGAGCCTCGTAAAGACCAGCGCAGCTACCTGGCGGACAGCTA
TCCCGTGGTATGGATGGGGAATGACGACCTTTCCCTGGTGCAGGCCGGCGCGGACGCCCGCCGGAAATACATGGACTTTC
TGGGCAGCCAGTGGCATCCAGGCTACAGACTGGCTCTGTTCAGCTACCGCCGGGCGCTGAAAACGCGCAATTACCTGCTC
AAGCACAGGCACCGGGACAAACTCCAGCTGGACGCCTACACGCGACAGCTGGCCCTGCACGGCACGGAACTGAGAAACCT
GCGCGCCAATCTGCTGGCGCTTCTGGCGCCCCATATCGCCCTGGCCTACCGCAATATCGGCGGAAGGCAGGAACAGGTCT
CCATCGCTTACCGTGCCTCGGAGGAAGGAGACCTGTATGAACGGCTGTGCGCCTCCATGGACCGGGATATCCGGTACGGA
CAAACCCAGAACGGCCCCCACCGGGATGACTTGGACATCACGCTGAACGGCAGAAACGCCGCTCAATTCGCCTCCGAAGG
GCAGCAGCGCACCACCGCCATCTCCATGAAGCTGGCGCAGTCCTCCCTCCTGACGGAAGAAACGGGACATACGCCCATCC
ACCTGATTGACGATGTCTTCGGGGAGCTGGACCCCACGCGCCGGATAGCCTTTCTGCAGTCGCTTCCAGCGGACGCGCAG
AGCCTCATCACCACCACCCATCTGGACTGGCTGCACAATGCTCCGTGCCCCCTGCCAGCCTTCCGGCTGGAGGATGGCGC
CCTTGCCCCCCTCTGA

Upstream 100 bases:

>100_bases
GAACTCAACGCCAAAGGAAAAGATGCATACCAGCACCTGACGGACAAAATCATGGACGCCATCCGCTCCCTCCCTGCGGA
ATAACCCCTTTCCCGGCCCC

Downstream 100 bases:

>100_bases
AAAAGCGCGGACAGAAACAACGAACAGGCGTCCGCCTGCGGCATTTCCGGGGCGCTATTCCTTCACCCATACGTTCAGGA
TTTCCACAATATACATCTTA

Product: DNA replication and repair protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MISRLKLMDFRCYGSFSWQIPQQGAIILGNNARGKTSLLEAVCFLLRLQSPRTARTGPLVSHGKQSFGIRGELPGQIRRI
LWAPDAPDLRVNGEPRKDQRSYLADSYPVVWMGNDDLSLVQAGADARRKYMDFLGSQWHPGYRLALFSYRRALKTRNYLL
KHRHRDKLQLDAYTRQLALHGTELRNLRANLLALLAPHIALAYRNIGGRQEQVSIAYRASEEGDLYERLCASMDRDIRYG
QTQNGPHRDDLDITLNGRNAAQFASEGQQRTTAISMKLAQSSLLTEETGHTPIHLIDDVFGELDPTRRIAFLQSLPADAQ
SLITTTHLDWLHNAPCPLPAFRLEDGALAPL

Sequences:

>Translated_351_residues
MISRLKLMDFRCYGSFSWQIPQQGAIILGNNARGKTSLLEAVCFLLRLQSPRTARTGPLVSHGKQSFGIRGELPGQIRRI
LWAPDAPDLRVNGEPRKDQRSYLADSYPVVWMGNDDLSLVQAGADARRKYMDFLGSQWHPGYRLALFSYRRALKTRNYLL
KHRHRDKLQLDAYTRQLALHGTELRNLRANLLALLAPHIALAYRNIGGRQEQVSIAYRASEEGDLYERLCASMDRDIRYG
QTQNGPHRDDLDITLNGRNAAQFASEGQQRTTAISMKLAQSSLLTEETGHTPIHLIDDVFGELDPTRRIAFLQSLPADAQ
SLITTTHLDWLHNAPCPLPAFRLEDGALAPL
>Mature_351_residues
MISRLKLMDFRCYGSFSWQIPQQGAIILGNNARGKTSLLEAVCFLLRLQSPRTARTGPLVSHGKQSFGIRGELPGQIRRI
LWAPDAPDLRVNGEPRKDQRSYLADSYPVVWMGNDDLSLVQAGADARRKYMDFLGSQWHPGYRLALFSYRRALKTRNYLL
KHRHRDKLQLDAYTRQLALHGTELRNLRANLLALLAPHIALAYRNIGGRQEQVSIAYRASEEGDLYERLCASMDRDIRYG
QTQNGPHRDDLDITLNGRNAAQFASEGQQRTTAISMKLAQSSLLTEETGHTPIHLIDDVFGELDPTRRIAFLQSLPADAQ
SLITTTHLDWLHNAPCPLPAFRLEDGALAPL

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=316, Percent_Identity=27.2151898734177, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 39607; Mature: 39607

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISRLKLMDFRCYGSFSWQIPQQGAIILGNNARGKTSLLEAVCFLLRLQSPRTARTGPLV
CCCCEEEEEEEEECCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCH
SHGKQSFGIRGELPGQIRRILWAPDAPDLRVNGEPRKDQRSYLADSYPVVWMGNDDLSLV
HCCCHHCCCCCCCCHHHHHEEECCCCCCEEECCCCCHHHHHHHHCCCCEEEECCCCHHHH
QAGADARRKYMDFLGSQWHPGYRLALFSYRRALKTRNYLLKHRHRDKLQLDAYTRQLALH
HCCHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
GTELRNLRANLLALLAPHIALAYRNIGGRQEQVSIAYRASEEGDLYERLCASMDRDIRYG
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCC
QTQNGPHRDDLDITLNGRNAAQFASEGQQRTTAISMKLAQSSLLTEETGHTPIHLIDDVF
CCCCCCCCCCCEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
GELDPTRRIAFLQSLPADAQSLITTTHLDWLHNAPCPLPAFRLEDGALAPL
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCC
>Mature Secondary Structure
MISRLKLMDFRCYGSFSWQIPQQGAIILGNNARGKTSLLEAVCFLLRLQSPRTARTGPLV
CCCCEEEEEEEEECCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCH
SHGKQSFGIRGELPGQIRRILWAPDAPDLRVNGEPRKDQRSYLADSYPVVWMGNDDLSLV
HCCCHHCCCCCCCCHHHHHEEECCCCCCEEECCCCCHHHHHHHHCCCCEEEECCCCHHHH
QAGADARRKYMDFLGSQWHPGYRLALFSYRRALKTRNYLLKHRHRDKLQLDAYTRQLALH
HCCHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
GTELRNLRANLLALLAPHIALAYRNIGGRQEQVSIAYRASEEGDLYERLCASMDRDIRYG
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCC
QTQNGPHRDDLDITLNGRNAAQFASEGQQRTTAISMKLAQSSLLTEETGHTPIHLIDDVF
CCCCCCCCCCCEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
GELDPTRRIAFLQSLPADAQSLITTTHLDWLHNAPCPLPAFRLEDGALAPL
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]