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The map label for this gene is mazG [H]

Identifier: 187735077

GI number: 187735077

Start: 669333

End: 670142

Strand: Direct

Name: mazG [H]

Synonym: Amuc_0571

Alternate gene names: 187735077

Gene position: 669333-670142 (Clockwise)

Preceding gene: 187735073

Following gene: 187735079

Centisome position: 25.12

GC content: 56.17

Gene sequence:

>810_bases
ATGAACGACACTGAAATGATCGAATGCCGCGAGCCTGCCCTTCAAATGCAGCGCCTCATCGCCATCATGAAACGTCTGCG
CGCGCCCCACGGCTGCCCCTGGGACGCGGAGCAAACCCACCATTCCCTGATTTCCAATATGATCGAGGAAGCCTATGAAG
TCGTGGACACCATTCAGCGGAATGACTGGACGCAGCTGAGGGAAGAATTGGGCGATGTTTTGCTTCAAGTGGTTTTTCAT
GCGGAGATTGCCCAGGAAGCAGGGCGTTTCGATTTCAATGACGTGGCTGCGGAAGTAAGCGAAAAACTCGTCCGCCGCCA
TCCCCACGTATTTGCCCAGTCCAAGGCGGACACGACGGATGCCGTATTAGCTCAGTGGGACAAGATCAAACGCCGGGAAA
AGGGGGCGGAAACAACCCCATACCTGCATGGAACAGGCAAGGGGCTGCCGCCCATGCTCCAAGCATGGAAGCTCCAGAAA
AAAGCCGCCAAAGTAGGATTTGACTGGGCGGACGCCCAAGGCGCCCTTGACAAGGTGAAGGAAGAAACCGCGGAATGCGG
GGAAATTCTTTCCGCACCGGAGGAAGACCCCCGCGTCGCGGAAGAATTGGGGGATCTTCTGTTTTCCGTAGTCAACCTGT
GCAGGAAAAAAGGAATCGACCCGGAAACGGCCATGGCCGGAGCAAACAGAAAATTTGAGCGGCGTTTCAACGAAATGGAA
CGGCTGCTTGCCAAAGACGGTCTTTCCCTGGAAGAAGCTTCTGCGGAGGCCATGGAGGCACGCTGGCAGCAGGCAAAATC
CGCCCGGTAA

Upstream 100 bases:

>100_bases
CGTGTGAACTTCCATGGAGGCGAGTGTAAGGAAGAAGCGTTTCGGGTAAAGAGGAATTTTTTCTAGTCATCCGCCCTTGC
GCGATTAGTATGGCTCCGTC

Downstream 100 bases:

>100_bases
CGGAATCCGGGGTCAGGCCATCATGTCCATGTTCGGGTCTTTCCAGGCCGGATCATATCCCTTGGCATAGCTGAACACAT
GCTTGTGCATGTATTCGAAG

Product: MazG family protein

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MNDTEMIECREPALQMQRLIAIMKRLRAPHGCPWDAEQTHHSLISNMIEEAYEVVDTIQRNDWTQLREELGDVLLQVVFH
AEIAQEAGRFDFNDVAAEVSEKLVRRHPHVFAQSKADTTDAVLAQWDKIKRREKGAETTPYLHGTGKGLPPMLQAWKLQK
KAAKVGFDWADAQGALDKVKEETAECGEILSAPEEDPRVAEELGDLLFSVVNLCRKKGIDPETAMAGANRKFERRFNEME
RLLAKDGLSLEEASAEAMEARWQQAKSAR

Sequences:

>Translated_269_residues
MNDTEMIECREPALQMQRLIAIMKRLRAPHGCPWDAEQTHHSLISNMIEEAYEVVDTIQRNDWTQLREELGDVLLQVVFH
AEIAQEAGRFDFNDVAAEVSEKLVRRHPHVFAQSKADTTDAVLAQWDKIKRREKGAETTPYLHGTGKGLPPMLQAWKLQK
KAAKVGFDWADAQGALDKVKEETAECGEILSAPEEDPRVAEELGDLLFSVVNLCRKKGIDPETAMAGANRKFERRFNEME
RLLAKDGLSLEEASAEAMEARWQQAKSAR
>Mature_269_residues
MNDTEMIECREPALQMQRLIAIMKRLRAPHGCPWDAEQTHHSLISNMIEEAYEVVDTIQRNDWTQLREELGDVLLQVVFH
AEIAQEAGRFDFNDVAAEVSEKLVRRHPHVFAQSKADTTDAVLAQWDKIKRREKGAETTPYLHGTGKGLPPMLQAWKLQK
KAAKVGFDWADAQGALDKVKEETAECGEILSAPEEDPRVAEELGDLLFSVVNLCRKKGIDPETAMAGANRKFERRFNEME
RLLAKDGLSLEEASAEAMEARWQQAKSAR

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=255, Percent_Identity=49.0196078431373, Blast_Score=241, Evalue=4e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 30436; Mature: 30436

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDTEMIECREPALQMQRLIAIMKRLRAPHGCPWDAEQTHHSLISNMIEEAYEVVDTIQR
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
NDWTQLREELGDVLLQVVFHAEIAQEAGRFDFNDVAAEVSEKLVRRHPHVFAQSKADTTD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCHHH
AVLAQWDKIKRREKGAETTPYLHGTGKGLPPMLQAWKLQKKAAKVGFDWADAQGALDKVK
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EETAECGEILSAPEEDPRVAEELGDLLFSVVNLCRKKGIDPETAMAGANRKFERRFNEME
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHH
RLLAKDGLSLEEASAEAMEARWQQAKSAR
HHHHHCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNDTEMIECREPALQMQRLIAIMKRLRAPHGCPWDAEQTHHSLISNMIEEAYEVVDTIQR
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
NDWTQLREELGDVLLQVVFHAEIAQEAGRFDFNDVAAEVSEKLVRRHPHVFAQSKADTTD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCHHH
AVLAQWDKIKRREKGAETTPYLHGTGKGLPPMLQAWKLQKKAAKVGFDWADAQGALDKVK
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EETAECGEILSAPEEDPRVAEELGDLLFSVVNLCRKKGIDPETAMAGANRKFERRFNEME
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHH
RLLAKDGLSLEEASAEAMEARWQQAKSAR
HHHHHCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]