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The map label for this gene is rng [H]

Identifier: 187735048

GI number: 187735048

Start: 638613

End: 640160

Strand: Direct

Name: rng [H]

Synonym: Amuc_0541

Alternate gene names: 187735048

Gene position: 638613-640160 (Clockwise)

Preceding gene: 187735047

Following gene: 187735049

Centisome position: 23.97

GC content: 56.91

Gene sequence:

>1548_bases
ATGGAAGGCTCCACTATCATCATCAATGCCGAAAAGCTCGAACGCCGCGTAGCCCTTCTGGAAAACGGCGTATTGGAAGA
ATACACCATCGAGCGCGAAGGCGAAGACAACATCGTAGGCGGCATTTTCAAAGGCCGTGTCAAGAACATCGAACCGGGCC
TCAAAGCCATGTTCGTGGACATCGGGCAGGAAAAAAACGCCTTTCTGCACTTCTGGGACGCGCTTCCCGCCGCACTGGAC
TCCAGCCTGGAAGAAATCGAACGCGAAGGCCGTCCGAAAAAACAGCAGCGCAAAATCACGTCCAAAGACATCCCGGACAT
CTATCCCATCGGCTCTGAAATCATGGTGCAGGTCACCAAAGGACCGATCGGCACCAAGGGTCCCCGTGTCACAACGAACG
TATCCCTGGCGGGACGCTACCTGGTCCTGATGCCTTTTACGGACCAGTTCGGCATCTCCCGTAAAATTGAAGACCCCAAA
GAGCGCCTGCGCCTGCGCCAGATCATGCAGAAACTGAATGTTCCGGACGGAATGGGCATCATCATGCGCACGGTGGCCCA
GGGCCAGCGCTACCGCCACTTTGTCCGCGACCTCGCCATGCTGCTGGAACAATGGTACAGCGTGGAAGAAAAGAAGGAGG
CCAATCCGGCCCCCGTCTGCGTGTACAAGGAGCCGGACCTGATTGAACGCACGGCACGGGATTTCCTTACGGACACCGTA
GACAACATCATCTGCGACAACGCGGAAACCACGGAACACATGCGCGCCATTGCCGGAAAAATCTCGCGCCGCGCCAAGCG
CCACATCCAGCACTTCCCCGTGCGCACGCCTATCTTTGAAGAACTGGGAATTGAAAAACAAATCAATGAAGCTTTCCAGC
GCCAGGTGCTCCTGCCCTGCGGAGGCTACCTGGTGATTGACGAAACGGAGGCGCTGATCGCCATTGACGTCAACACCGGA
CGCAACAAGGGCACCAAGGATCTGGACAAGACCATTCTGGATACCAATCTGGAAGCCGCCTATGAAATCGCCCGCCAGCT
TCGCCTGCGCAACATCGGCGGTCTGGTGGTGGTGGACTTTATCGACATGCGCCACAGGAAGGACCAGCAGGCCGTTTATA
AGGCCATGAAGGACCGCCTGAAACGGGACAAGGCAAAAACCCAGGTACTGCAGATCACCCCCATCGGCCTGATGGAAATG
ACGCGCCAGCGGCTCAACGAGAGCCTCAGGGAATCCGTTCATGATCCGTGCCCGTACTGCGGAGGCAGGGGCCGCGTCAA
ATCCGTGATGACCATGAGCGTGGAAATTCAGCGGCAAATTTACGCCTGCATCCAGAAATACCGGGATACCATCGGAGACA
TGGTCATCGTCGTCAACTCGGAAGTGCTCTCCCGCTTCAAGAATGAAGACGCCAGCCTGCTCATGGAACTGGAACGCCAG
CACAGCGGAAGGCTGATTTTCCGGGGCGACCACAACCAGCACCGCGAATCCTTCGCCATTTACGACGCCCGATACGACAA
CAAACCCCTCTACCAGTCCGGGCAATAG

Upstream 100 bases:

>100_bases
ACATTACGGGCAATAGCACACCCTAGCTTATTCTCACCCCGTGTTTACAAAAATGCTTAAAAAAATCAAACGGGCCTTCG
GCTTCGGATCCTGCGATCCC

Downstream 100 bases:

>100_bases
CCTGAAAATCAAATCCGGCTTTCCGGGCCGGGCGATACCCTCGCCCGGCCTTTTTTACGCCGCCATGAACAACATTCCCA
CTTCCCCCAACGACCCCGTC

Product: ribonuclease, Rne/Rng family

Products: NA

Alternate protein names: RNase G; Cytoplasmic axial filament protein [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MEGSTIIINAEKLERRVALLENGVLEEYTIEREGEDNIVGGIFKGRVKNIEPGLKAMFVDIGQEKNAFLHFWDALPAALD
SSLEEIEREGRPKKQQRKITSKDIPDIYPIGSEIMVQVTKGPIGTKGPRVTTNVSLAGRYLVLMPFTDQFGISRKIEDPK
ERLRLRQIMQKLNVPDGMGIIMRTVAQGQRYRHFVRDLAMLLEQWYSVEEKKEANPAPVCVYKEPDLIERTARDFLTDTV
DNIICDNAETTEHMRAIAGKISRRAKRHIQHFPVRTPIFEELGIEKQINEAFQRQVLLPCGGYLVIDETEALIAIDVNTG
RNKGTKDLDKTILDTNLEAAYEIARQLRLRNIGGLVVVDFIDMRHRKDQQAVYKAMKDRLKRDKAKTQVLQITPIGLMEM
TRQRLNESLRESVHDPCPYCGGRGRVKSVMTMSVEIQRQIYACIQKYRDTIGDMVIVVNSEVLSRFKNEDASLLMELERQ
HSGRLIFRGDHNQHRESFAIYDARYDNKPLYQSGQ

Sequences:

>Translated_515_residues
MEGSTIIINAEKLERRVALLENGVLEEYTIEREGEDNIVGGIFKGRVKNIEPGLKAMFVDIGQEKNAFLHFWDALPAALD
SSLEEIEREGRPKKQQRKITSKDIPDIYPIGSEIMVQVTKGPIGTKGPRVTTNVSLAGRYLVLMPFTDQFGISRKIEDPK
ERLRLRQIMQKLNVPDGMGIIMRTVAQGQRYRHFVRDLAMLLEQWYSVEEKKEANPAPVCVYKEPDLIERTARDFLTDTV
DNIICDNAETTEHMRAIAGKISRRAKRHIQHFPVRTPIFEELGIEKQINEAFQRQVLLPCGGYLVIDETEALIAIDVNTG
RNKGTKDLDKTILDTNLEAAYEIARQLRLRNIGGLVVVDFIDMRHRKDQQAVYKAMKDRLKRDKAKTQVLQITPIGLMEM
TRQRLNESLRESVHDPCPYCGGRGRVKSVMTMSVEIQRQIYACIQKYRDTIGDMVIVVNSEVLSRFKNEDASLLMELERQ
HSGRLIFRGDHNQHRESFAIYDARYDNKPLYQSGQ
>Mature_515_residues
MEGSTIIINAEKLERRVALLENGVLEEYTIEREGEDNIVGGIFKGRVKNIEPGLKAMFVDIGQEKNAFLHFWDALPAALD
SSLEEIEREGRPKKQQRKITSKDIPDIYPIGSEIMVQVTKGPIGTKGPRVTTNVSLAGRYLVLMPFTDQFGISRKIEDPK
ERLRLRQIMQKLNVPDGMGIIMRTVAQGQRYRHFVRDLAMLLEQWYSVEEKKEANPAPVCVYKEPDLIERTARDFLTDTV
DNIICDNAETTEHMRAIAGKISRRAKRHIQHFPVRTPIFEELGIEKQINEAFQRQVLLPCGGYLVIDETEALIAIDVNTG
RNKGTKDLDKTILDTNLEAAYEIARQLRLRNIGGLVVVDFIDMRHRKDQQAVYKAMKDRLKRDKAKTQVLQITPIGLMEM
TRQRLNESLRESVHDPCPYCGGRGRVKSVMTMSVEIQRQIYACIQKYRDTIGDMVIVVNSEVLSRFKNEDASLLMELERQ
HSGRLIFRGDHNQHRESFAIYDARYDNKPLYQSGQ

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=473, Percent_Identity=38.0549682875264, Blast_Score=307, Evalue=9e-85,
Organism=Escherichia coli, GI1787325, Length=481, Percent_Identity=38.045738045738, Blast_Score=306, Evalue=2e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: 3.1.4.- [C]

Molecular weight: Translated: 59070; Mature: 59070

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGSTIIINAEKLERRVALLENGVLEEYTIEREGEDNIVGGIFKGRVKNIEPGLKAMFVD
CCCCEEEEEHHHHHHHHHHHHCCCHHHEEECCCCCCCEECHHHHCCCCCCCCCHHHHHEE
IGQEKNAFLHFWDALPAALDSSLEEIEREGRPKKQQRKITSKDIPDIYPIGSEIMVQVTK
CCCCCCHHEEHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEEC
GPIGTKGPRVTTNVSLAGRYLVLMPFTDQFGISRKIEDPKERLRLRQIMQKLNVPDGMGI
CCCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
IMRTVAQGQRYRHFVRDLAMLLEQWYSVEEKKEANPAPVCVYKEPDLIERTARDFLTDTV
HHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHH
DNIICDNAETTEHMRAIAGKISRRAKRHIQHFPVRTPIFEELGIEKQINEAFQRQVLLPC
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHCEEEECC
GGYLVIDETEALIAIDVNTGRNKGTKDLDKTILDTNLEAAYEIARQLRLRNIGGLVVVDF
CCEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEH
IDMRHRKDQQAVYKAMKDRLKRDKAKTQVLQITPIGLMEMTRQRLNESLRESVHDPCPYC
HHHHCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCC
GGRGRVKSVMTMSVEIQRQIYACIQKYRDTIGDMVIVVNSEVLSRFKNEDASLLMELERQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHCCCHHHHHHHHHHH
HSGRLIFRGDHNQHRESFAIYDARYDNKPLYQSGQ
HCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MEGSTIIINAEKLERRVALLENGVLEEYTIEREGEDNIVGGIFKGRVKNIEPGLKAMFVD
CCCCEEEEEHHHHHHHHHHHHCCCHHHEEECCCCCCCEECHHHHCCCCCCCCCHHHHHEE
IGQEKNAFLHFWDALPAALDSSLEEIEREGRPKKQQRKITSKDIPDIYPIGSEIMVQVTK
CCCCCCHHEEHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEEC
GPIGTKGPRVTTNVSLAGRYLVLMPFTDQFGISRKIEDPKERLRLRQIMQKLNVPDGMGI
CCCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
IMRTVAQGQRYRHFVRDLAMLLEQWYSVEEKKEANPAPVCVYKEPDLIERTARDFLTDTV
HHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHH
DNIICDNAETTEHMRAIAGKISRRAKRHIQHFPVRTPIFEELGIEKQINEAFQRQVLLPC
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHCEEEECC
GGYLVIDETEALIAIDVNTGRNKGTKDLDKTILDTNLEAAYEIARQLRLRNIGGLVVVDF
CCEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEH
IDMRHRKDQQAVYKAMKDRLKRDKAKTQVLQITPIGLMEMTRQRLNESLRESVHDPCPYC
HHHHCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCC
GGRGRVKSVMTMSVEIQRQIYACIQKYRDTIGDMVIVVNSEVLSRFKNEDASLLMELERQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHCCCHHHHHHHHHHH
HSGRLIFRGDHNQHRESFAIYDARYDNKPLYQSGQ
HCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]