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The map label for this gene is rffA [H]

Identifier: 187735008

GI number: 187735008

Start: 591093

End: 592232

Strand: Reverse

Name: rffA [H]

Synonym: Amuc_0501

Alternate gene names: 187735008

Gene position: 592232-591093 (Counterclockwise)

Preceding gene: 187735009

Following gene: 187735007

Centisome position: 22.23

GC content: 57.98

Gene sequence:

>1140_bases
ATGGAGATTCCTTTCAACCAACCTCACCAGCACGGTCCCGAATTGGATTATATCCGGGACGCCATCCAGCGCGCCCATTT
ATGCGGAGACGGCTATTACACGAAAAAATGCCATGAACTGCTTGCGGAACGCACCGGCGCCGGAAAGGTTCTGCTGGTTC
ATTCCGGAACCGCCGCGCTGGAGATGGCGGCCCTGTTGATCGACTGCCGCCCGGGCGACGAGATTATCATGCCTTCCTAT
ACGTTCGTCTCCACGGCGAACGCGTTTGTGCTGCGCGGCGGCGTTCCTGTATTCGTGGATATCAGGCCGGATACGCAGAA
TATTGACGAGACGCTGATAGAAGCGGCCATCACGGACCGCACCCGCGCCATTTGCGTGGTTCATTACGCGGGCGTGGCTT
GTGAAATGGATGCAATCATGGAAATTGCCGAGAGGCATCGCCTGTATGTGATTGAAGACGCTGCCCAGGGCGTCGGCTCC
TGCTACAGAGGGCGTCGGCTCGGCTCCATCGGCCATTTGGGCTGTTACAGTTTCCACGAGACCAAGAATGTCATTTCCGG
AGAGGGAGGAGCCCTGCTGGTGAATGACCCCTCCCTGATGGAACGCGCGGAGATTATCCGGGAAAAAGGGACCAACCGTG
CCAGGTTTTTCCGCGGGCAGGTGGACAAATACACCTGGGTGGACGTAGGTTCCTCCTATCTGCCCGGAGAGATGATCGCC
GCTTATTTGTATGCCCAGCTGGAACACGGGCAGGAGATCAACGGGGAAAGGCTGAAGTGGTGGGATATGTACCACCGCGC
GCTGGCTCCATTGGAAGAACAGGGGGTGTTGCGGCGTCCGTGCATTCCGGAGCATTGCACGCATAACGCCCACATGTATT
ACGTGTTGCTCCGTTCCATGGAGGAGCGCACGCGCTTGATCAACAAGCTGGCGGAGCACGGGATTAAAGCGGTGTTCCAC
TACATTCCCCTCCATTCCTCCCCTGCCGGGATGAGATTCGGGCGTTCTGCCGGGGCCATGCCTCATACGGACCGTACCAG
CGATACGTTGCTGAGGCTTCCTTTGTATTACGATTTGGGGGAAGAAGGTTGCCGGGAGGTGTTGCGGGCCGGCTTCGGGC
TGGAGGGAGTTGAATCATGA

Upstream 100 bases:

>100_bases
CATAGTAGGGTCTCCCCGTGCGGAAGGCGGGGAACGCCTTCCGCACGGGATCGGCATGCCCGCGCCGGGCGTCCGTCATG
GGCCGGTCTTTTCTGAAGCC

Downstream 100 bases:

>100_bases
TGAAAATTTGCCTGGTCAACGTGTCTGATGTGGATGAGGCCATGCGCAGCCGCCTGCGCGTGTGGCGCAATTCCGAAAAG
ATCACCCCGTTTTTTTTGTT

Product: TDP-4-oxo-6-deoxy-D-glucose transaminase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MEIPFNQPHQHGPELDYIRDAIQRAHLCGDGYYTKKCHELLAERTGAGKVLLVHSGTAALEMAALLIDCRPGDEIIMPSY
TFVSTANAFVLRGGVPVFVDIRPDTQNIDETLIEAAITDRTRAICVVHYAGVACEMDAIMEIAERHRLYVIEDAAQGVGS
CYRGRRLGSIGHLGCYSFHETKNVISGEGGALLVNDPSLMERAEIIREKGTNRARFFRGQVDKYTWVDVGSSYLPGEMIA
AYLYAQLEHGQEINGERLKWWDMYHRALAPLEEQGVLRRPCIPEHCTHNAHMYYVLLRSMEERTRLINKLAEHGIKAVFH
YIPLHSSPAGMRFGRSAGAMPHTDRTSDTLLRLPLYYDLGEEGCREVLRAGFGLEGVES

Sequences:

>Translated_379_residues
MEIPFNQPHQHGPELDYIRDAIQRAHLCGDGYYTKKCHELLAERTGAGKVLLVHSGTAALEMAALLIDCRPGDEIIMPSY
TFVSTANAFVLRGGVPVFVDIRPDTQNIDETLIEAAITDRTRAICVVHYAGVACEMDAIMEIAERHRLYVIEDAAQGVGS
CYRGRRLGSIGHLGCYSFHETKNVISGEGGALLVNDPSLMERAEIIREKGTNRARFFRGQVDKYTWVDVGSSYLPGEMIA
AYLYAQLEHGQEINGERLKWWDMYHRALAPLEEQGVLRRPCIPEHCTHNAHMYYVLLRSMEERTRLINKLAEHGIKAVFH
YIPLHSSPAGMRFGRSAGAMPHTDRTSDTLLRLPLYYDLGEEGCREVLRAGFGLEGVES
>Mature_379_residues
MEIPFNQPHQHGPELDYIRDAIQRAHLCGDGYYTKKCHELLAERTGAGKVLLVHSGTAALEMAALLIDCRPGDEIIMPSY
TFVSTANAFVLRGGVPVFVDIRPDTQNIDETLIEAAITDRTRAICVVHYAGVACEMDAIMEIAERHRLYVIEDAAQGVGS
CYRGRRLGSIGHLGCYSFHETKNVISGEGGALLVNDPSLMERAEIIREKGTNRARFFRGQVDKYTWVDVGSSYLPGEMIA
AYLYAQLEHGQEINGERLKWWDMYHRALAPLEEQGVLRRPCIPEHCTHNAHMYYVLLRSMEERTRLINKLAEHGIKAVFH
YIPLHSSPAGMRFGRSAGAMPHTDRTSDTLLRLPLYYDLGEEGCREVLRAGFGLEGVES

Specific function: Involved in ECA elongation [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI2367285, Length=367, Percent_Identity=62.125340599455, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI145693159, Length=382, Percent_Identity=29.3193717277487, Blast_Score=151, Evalue=8e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR012749
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 42489; Mature: 42489

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIPFNQPHQHGPELDYIRDAIQRAHLCGDGYYTKKCHELLAERTGAGKVLLVHSGTAAL
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCHHHH
EMAALLIDCRPGDEIIMPSYTFVSTANAFVLRGGVPVFVDIRPDTQNIDETLIEAAITDR
HHHHHHEECCCCCCEECCCCHHHHCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHCC
TRAICVVHYAGVACEMDAIMEIAERHRLYVIEDAAQGVGSCYRGRRLGSIGHLGCYSFHE
CCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCHHH
TKNVISGEGGALLVNDPSLMERAEIIREKGTNRARFFRGQVDKYTWVDVGSSYLPGEMIA
HHHHCCCCCCEEEECCHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCCCCCHHHHH
AYLYAQLEHGQEINGERLKWWDMYHRALAPLEEQGVLRRPCIPEHCTHNAHMYYVLLRSM
HHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHCCCCHHHHHHHHHH
EERTRLINKLAEHGIKAVFHYIPLHSSPAGMRFGRSAGAMPHTDRTSDTLLRLPLYYDLG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHEECCC
EEGCREVLRAGFGLEGVES
HHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MEIPFNQPHQHGPELDYIRDAIQRAHLCGDGYYTKKCHELLAERTGAGKVLLVHSGTAAL
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCHHHH
EMAALLIDCRPGDEIIMPSYTFVSTANAFVLRGGVPVFVDIRPDTQNIDETLIEAAITDR
HHHHHHEECCCCCCEECCCCHHHHCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHCC
TRAICVVHYAGVACEMDAIMEIAERHRLYVIEDAAQGVGSCYRGRRLGSIGHLGCYSFHE
CCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCHHH
TKNVISGEGGALLVNDPSLMERAEIIREKGTNRARFFRGQVDKYTWVDVGSSYLPGEMIA
HHHHCCCCCCEEEECCHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCCCCCHHHHH
AYLYAQLEHGQEINGERLKWWDMYHRALAPLEEQGVLRRPCIPEHCTHNAHMYYVLLRSM
HHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHCCCCHHHHHHHHHH
EERTRLINKLAEHGIKAVFHYIPLHSSPAGMRFGRSAGAMPHTDRTSDTLLRLPLYYDLG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHEECCC
EEGCREVLRAGFGLEGVES
HHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 8366065 [H]