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The map label for this gene is rph [H]

Identifier: 187734903

GI number: 187734903

Start: 477078

End: 477890

Strand: Reverse

Name: rph [H]

Synonym: Amuc_0395

Alternate gene names: 187734903

Gene position: 477890-477078 (Counterclockwise)

Preceding gene: 187734905

Following gene: 187734902

Centisome position: 17.94

GC content: 58.79

Gene sequence:

>813_bases
ATGACGGCATCCGTCATGATTCCCCGCATGGAACGTCAGGATAAACGACTTGTGGACCAGTTGCGTCCGATCAGCTTTGA
AACGGGTATCGCCCCGAATGCCACGGCTTCCGTGCTGGTGACTTTCGGACGCACCAAGGTGATTTGCGCCGTTACGATTG
AGGAGGATGTACCCCGCTGGATGAAGGTTCAGCGTGTGGAGGGAGGATGGCTGACGGCGGAGTATTCCATGCTTCCCTAT
TCCACGCTTGACCGTAAACGCCGGGATATTACGGCGGGGAAACTGGACGGACGTTCCAGCGAGATCCAGCGTCTGATCGG
CCGTTCCCTGAGGGCGGCCGTGGATCTGGGCAAGATCGGCCAGCGCACGATTTGGGTGGATTGCGACGTTTTACAGGCTG
ATGGCGGTACCCGCACCGCTTCCATTACCGGGGCTTCCGTAGCTCTGGCCATTGCCGTGAACAAGCTTGTCGCGGAAGGA
AAGCTGGCGGAGTCTCCCATGAAGAGGCTGGTTTCCGCCGTATCCGTGGGAATACTGGAGGGAGAGGCTCTGCTGGATCT
CTGCTATGTGGAGGATAAGGATGCGGAAGTGGATATGAATCTGGTGATGACCGACCAGGGAGAGTTTGTGGAGGTGCAGG
GGTCCGGTGAAGAGGCCGTGTTCACGGCGGATCAGATGAACCGGATGCTGGAATTAGGCCGCAAGGGGCTGGAAGAGATC
GCGGAACTCCAGCGGCAGGTGATTGCCGAGGAGGACAGGCCGTCCGCCGGAGCTTTGGAAGGGTTGAGTTCCTTTTTCGG
CAGCGGCAGGTAG

Upstream 100 bases:

>100_bases
AGGGCCACCACGTCAATGACGGCCACACGGGTGCGAGGGTGTTTCATGGGAGGGATATTAGCGGTTTGGAACGGGAATTC
CATCTTTCCATTTGAAAAAG

Downstream 100 bases:

>100_bases
GATTTTTCCCCATGGCCCGGTATTTTCCGGGCCTTTTGCAGGGGGGCTGCGGCAGAGTTTTCACTCCTTGCAGGACCAAG
ATTTTTATGAGAATATGCTT

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MTASVMIPRMERQDKRLVDQLRPISFETGIAPNATASVLVTFGRTKVICAVTIEEDVPRWMKVQRVEGGWLTAEYSMLPY
STLDRKRRDITAGKLDGRSSEIQRLIGRSLRAAVDLGKIGQRTIWVDCDVLQADGGTRTASITGASVALAIAVNKLVAEG
KLAESPMKRLVSAVSVGILEGEALLDLCYVEDKDAEVDMNLVMTDQGEFVEVQGSGEEAVFTADQMNRMLELGRKGLEEI
AELQRQVIAEEDRPSAGALEGLSSFFGSGR

Sequences:

>Translated_270_residues
MTASVMIPRMERQDKRLVDQLRPISFETGIAPNATASVLVTFGRTKVICAVTIEEDVPRWMKVQRVEGGWLTAEYSMLPY
STLDRKRRDITAGKLDGRSSEIQRLIGRSLRAAVDLGKIGQRTIWVDCDVLQADGGTRTASITGASVALAIAVNKLVAEG
KLAESPMKRLVSAVSVGILEGEALLDLCYVEDKDAEVDMNLVMTDQGEFVEVQGSGEEAVFTADQMNRMLELGRKGLEEI
AELQRQVIAEEDRPSAGALEGLSSFFGSGR
>Mature_269_residues
TASVMIPRMERQDKRLVDQLRPISFETGIAPNATASVLVTFGRTKVICAVTIEEDVPRWMKVQRVEGGWLTAEYSMLPYS
TLDRKRRDITAGKLDGRSSEIQRLIGRSLRAAVDLGKIGQRTIWVDCDVLQADGGTRTASITGASVALAIAVNKLVAEGK
LAESPMKRLVSAVSVGILEGEALLDLCYVEDKDAEVDMNLVMTDQGEFVEVQGSGEEAVFTADQMNRMLELGRKGLEEIA
ELQRQVIAEEDRPSAGALEGLSSFFGSGR

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Homo sapiens, GI9506689, Length=246, Percent_Identity=26.4227642276423, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=57.8199052132701, Blast_Score=231, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI71981632, Length=183, Percent_Identity=29.5081967213115, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 29437; Mature: 29306

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASVMIPRMERQDKRLVDQLRPISFETGIAPNATASVLVTFGRTKVICAVTIEEDVPRW
CCCCEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCEEEEEEEECCCCCHH
MKVQRVEGGWLTAEYSMLPYSTLDRKRRDITAGKLDGRSSEIQRLIGRSLRAAVDLGKIG
HHHEECCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
QRTIWVDCDVLQADGGTRTASITGASVALAIAVNKLVAEGKLAESPMKRLVSAVSVGILE
CEEEEEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
GEALLDLCYVEDKDAEVDMNLVMTDQGEFVEVQGSGEEAVFTADQMNRMLELGRKGLEEI
CHHHEEEEEECCCCCCEEEEEEEECCCCEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHH
AELQRQVIAEEDRPSAGALEGLSSFFGSGR
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TASVMIPRMERQDKRLVDQLRPISFETGIAPNATASVLVTFGRTKVICAVTIEEDVPRW
CCCEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCEEEEEEEECCCCCHH
MKVQRVEGGWLTAEYSMLPYSTLDRKRRDITAGKLDGRSSEIQRLIGRSLRAAVDLGKIG
HHHEECCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
QRTIWVDCDVLQADGGTRTASITGASVALAIAVNKLVAEGKLAESPMKRLVSAVSVGILE
CEEEEEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
GEALLDLCYVEDKDAEVDMNLVMTDQGEFVEVQGSGEEAVFTADQMNRMLELGRKGLEEI
CHHHEEEEEECCCCCCEEEEEEEECCCCEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHH
AELQRQVIAEEDRPSAGALEGLSSFFGSGR
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA